• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.93.2-93.2
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• 2003

To get a better insight into the biophysical mechanism of action of chlorhexidine digluconate, we examined the effect of chlorhexidine digluconate on the thickness of outer membranes isolated from cultured Porphyromonas gingivalis using energy transfer between the membrane surface fluorescent probe (l-anilinonaphthalene-8-sulfonic acid) and the hydrophobic fluorescent probe [1,3-di(l-pyrenyl)propane]. 1-Anilinonaphthalene-8-sulfonic acid quenches the monomer fluorescence of 1,3-di(1-pyrenyl)propane. (omitted)

• IMMUNE NETWORK
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• 제9권5호
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• pp.203-207
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• 2009

Background: The FimA of Porphyromonas gingivalis is a crucial pathogenic component of the bacteria and has been implicated as a target for vaccine development against the periodontal diseases. Methods: In this study, the purified fimbriae (FimA subunit polymers) protein was used for immunization in their native form and B hybridoma clones producing antibodies specific to FimA were established. Results: The monoclonal antibodies prepared from selected two clones, designated #123 (IgG2b/ kappa) and #265 (IgG1/kappa), displayed different patterns of binding activity against the cognate antigen. Both antibodies reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the monoclonal antibodies showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. Conclusion: These results suggest that the monoclonal antibodies could be used as vaccine material against the periodontal diseases through passive immunization.

• Preventive Nutrition and Food Science
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• 제7권2호
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• pp.215-218
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• 2002

A methanol extract of Physalis angulata exhibited in vitro antibarterial activity against oral pathogens such including Streptococcus mutans and Porphyromonas gingivalis. The methanol extract of Physalis angulata was further fractionated with ethyl acetate, n-butanol and water, in which the ethyl acetate fraction exclusively showed antibacterial activity. An active antibacterial compound from the ethyl acetate fraction was purified to a single compound using silica gel column chromatography and identified as oleanolic acid by $^{13}$ C-NMR, $^1$H-NMR and EI-MS. MIC of oleanolic acid against S. mutants and p. gingivalis were determined to be 50 and 25 ug/mL, respectively. The Antibacterial activity of oleanolic acid from Physalis angulata suggested that it has potential as an anticarcinogenic and antiperiodontic ingredients in various foods and oral care products.

• International Journal of Clinical Preventive Dentistry
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• 제14권4호
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• pp.216-221
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• 2018

Objective: In this study, we tried to investigate the antimicrobial activity of natural herbaceous plant-derived essential oils against oral disease-causing bacterial strains and establish the scientific evidences to apply them to the dentifrice, mouthwash, and other uses. Methods: We used the disk diffusion method with 23 types of 100% natural essential oils to verify the antimicrobial effect against Streptococcus mutans, Porphyromonas gingivalis, and Lactobacillus rhamnosus. After adhering to the surface of the plate culture medium, $20{\mu}l$ of the essential oil was prepared, and the diameter of the transparent ring was measured after incubation for 24 hours. All the experiments were repeated three times. Results: Among 23 types of oils, 13 were effective against all three strains: myrrh, ginger, basil, carrot seed, tea tree, patchouli, ylang ylang, cypress, lemongrass, cinnamon, peppermint, lavender, and eucalryptus. Seventeen oils were effective against S. mutans, and myrrh, basil, and carrot seed showed high antimicrobial activity. Eighteen oils were effective against P. gingivalis, and tea tree, carrot seed, and cinnamons showed high antimicrobial activity. Sixteen oils were effective against L. rhamnosus, and carrot seed and peppermint cinnamon showed high antimicrobial activity. Conclusion: Consequently, 13 essential oils showed the antimicrobial activity against three bacterial strains, which indicates these essential oils might be used as the effective materials to suppress the growth of oral-disease inducing microorganisms. Moreover, essential oils that have been analyzed activities in this study will be mixed up within the constant range and analyzed their antimicrobial effects to examine the synergistic activity among them.

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2005년도 제45회 종합학술대회 연제초록
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• pp.153-153
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• 2005

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2005년도 제45회 종합학술대회 연제초록
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• pp.91-91
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• 2005

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2002년도 제42회 종합학술대회 연제초록
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• pp.107-107
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• 2002

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 2002년도 제42회 종합학술대회 연제초록
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• pp.98-98
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• 2002

• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 연합학술대회
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• pp.282.1-282.1
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• 2003

• International Journal of Oral Biology
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• 제44권4호
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• pp.152-159
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• 2019

The oral care probiotic strain Weissella cibaria CMU (oraCMU) inhibits volatile sulphur compounds associated with halitosis, presumably by inhibiting the growth of associated oral pathogens. In the present study, we investigated whether oraCMU inhibits the production of these compounds by suppressing the expression of mgl. This gene encodes L-methionine-α-deamino-γ-mercaptomethane-lyase (METase) and is involved in the production of methyl mercaptan (CH₃SH) by Porphyromonas gingivalis. Therefore, we specifically investigated the effects of oraCMU on the growth, CH₃SH production, METase activity, and mgl expression of P. gingivalis. The minimum inhibitory concentrations of cell-free supernatant and secreted proteins from oraCMU were 125 mg/mL and 800 ㎍/mL, respectively. At sub-minimum inhibitory concentration levels, these metabolites inhibited CH₃SH production, but they also reduced P. gingivalis viability. Only heat-killed oraCMU decreased CH₃SH production without affecting P. gingivalis viability. Heat-killed oraCMU also inhibited METase activity toward L-methionine and mgl mRNA expression (p < 0.05). In summary, we demonstrated the inhibition of volatile sulphur compounds via the antimicrobial action of oraCMU and, for the first time, the inhibition of such compounds by heat-killed oraCMU, which occurred at the molecular level.