International Journal of Oral Biology

  • 제44권4호
  • /
  • Pages.152-159
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  • 2019
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  • 1226-7155(pISSN)
  • /
  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Weissella cibaria CMU suppresses mgl gene expression and enzyme activity associated with bad breath

  • Kim, Hyun-Jin (Institute of Biomaterial Implant, Department of Oral Anatomy, School of Dentistry, Wonkwang University) ;
  • Yeu, Ji-Eun (Research Institute, OraPharm Inc) ;
  • Lee, Dong-Suk (School of Nursing, Kangwon National University) ;
  • Kang, Mi-Sun (Research Institute, OraPharm Inc)
  • 투고 : 2019.09.23
  • 심사 : 2019.11.20
  • 발행 : 2019.12.31
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초록

The oral care probiotic strain Weissella cibaria CMU (oraCMU) inhibits volatile sulphur compounds associated with halitosis, presumably by inhibiting the growth of associated oral pathogens. In the present study, we investigated whether oraCMU inhibits the production of these compounds by suppressing the expression of mgl. This gene encodes L-methionine-α-deamino-γ-mercaptomethane-lyase (METase) and is involved in the production of methyl mercaptan (CH3SH) by Porphyromonas gingivalis. Therefore, we specifically investigated the effects of oraCMU on the growth, CH3SH production, METase activity, and mgl expression of P. gingivalis. The minimum inhibitory concentrations of cell-free supernatant and secreted proteins from oraCMU were 125 mg/mL and 800 ㎍/mL, respectively. At sub-minimum inhibitory concentration levels, these metabolites inhibited CH3SH production, but they also reduced P. gingivalis viability. Only heat-killed oraCMU decreased CH3SH production without affecting P. gingivalis viability. Heat-killed oraCMU also inhibited METase activity toward L-methionine and mgl mRNA expression (p < 0.05). In summary, we demonstrated the inhibition of volatile sulphur compounds via the antimicrobial action of oraCMU and, for the first time, the inhibition of such compounds by heat-killed oraCMU, which occurred at the molecular level.

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참고문헌

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