• 한국농림기상학회지
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• 제16권4호
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• pp.368-383
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• 2014

기후변화는 곤충의 밀도와 분포, 몸의 형태와 개체의 크기 등 생물학적인 형질 변화, 생식 및 유전적 특성, 그리고 멸종 등에 영향을 미칠 것으로 예상되고 있다. 기후변화의 영향을 예측하여 피해를 줄이기 위해서는 분산되어 있는 곤충 종별 기본적인 생물학적/생태학적 정보들을 종합하여 검토할 필요가 있다. 따라서 본 연구는 기후 및 환경의 변화에 대한 곤충, 특히 해충의 발생 변화 예측에 필요한 생물학적 정보를 정리하여 이를 활용한 미래 피해 예측을 위한 기초 자료를 제공하고자 수행하였다. 또한 국내외 문헌을 비교 분석하여 국내에서 기후변화 연구를 수행하는데 있어 제한 요인들을 확인하고 향후 필요한 연구소요를 제시하고자 하였다. 국내의 연구들은 단기 모니터링 자료를 이용하여 환경 요인과의 관계를 분석하는 수준에 그치고 있는 반면, 국외 연구들은 장기 모니터링 자료를 이용한 분포 변화 분석이나 기 개발된 생물 종의 파라미터를 이용한 발생 및 분포 변화 예측 그리고 곤충 군집의 구성 변화를 모니터링하는 등 다양한 내용을 주제로 기후변화의 영향을 연구하고 있었다. 결론적으로 기후변화에 대응하기 위해서는 체계적인 모니터링 기술 개발을 통해 곤충의 계절발생, 분포, 월동 특성 및 유전적 구조 변화에 대한 연구가 필수적이며, 주요 해충 및 잠재적인 해충에 대한 기후변화의 영향을 예측하기 위해 곤충 개체군 모델의 개발 역시 중요한 부분이 될 것이다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.10021-10025
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• 2014

Background: The study aimed to describe the age distribution of breast cancer diagnosis among Chinese females for comparison with the United States and the European Union, and provide evidence for the screening target population in China. Materials and Methods: Median age was estimated from hospital databases from 7 tertiary hospitals in China. Population-based data in China, United States and European Union was extracted from the National Central Cancer Registry, SEER program and GLOBOCAN 2008, respectively. Age-standardized distribution of breast cancer at diagnosis in the 3 areas was estimated based on the World Standard Population 2000. Results: The median age of breast cancer at diagnosis was around 50 in China, nearly 10 years earlier than United States and European Union. The diagnosis age in China did not vary between subgroups of calendar year, region and pathological characteristics. With adjustment for population structure, median age of breast cancer at diagnosis was 50~54 in China, but 55~59 in United States and European Union. Conclusions: The median diagnosis age of female breast cancer is much earlier in China than in the United States and the European Union pointing to racial differences in genetics and lifestyle. Screening programs should start at an earlier age for Chinese women and age disparities between Chinese and Western women warrant further studies.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6391-6395
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• 2012

Background: Ovarian cancer is ranked as the fifth most common cause of cancer death in women. In Malaysia, it is the fourth most common cancer in females. CA125 has been the tumor marker of choice in ovarian cancer but its diagnostic specificity in early stages is only 50%. Hence, there is a critical need to identify an alternative tumor marker that is capable of detecting detect ovarian cancer at an early stage. HE4 is a new tumor marker proposed for the early diagnosis of ovarian cancer and disease recurrence. Currently, none of the normal ranges of HE4 quoted in the literature are based on data for a multiethnic Asian population. Therefore, the aim of this study was to determine reference intervals for HE4 in an Asian population presenting in University Malaya Medical Centre, a tertiary reference hospital. Materials and Methods: 300 healthy women were recruited comprising 150 premenopausal and 150 postmenopausal women, aged from 20-76 years. All women were subjected to a pelvic ultrasonograph and were confirmed to be free from ovarian pathology on recruitment. Serum HE4 levels were determined by chemiluminescent microparticle immunoassay (CMIA, Abbott Architect). The reference intervals were determined following CLSI guidelines (C28-A2) using a non-parametric method. Results: The upper limits of the $95^{th}$ percentile reference interval (90%CI) for all the women collectively were 64.6 pmol/L, and 58.4 pmol/L for premenopausal) and 69.0 pmol/L for postmenopausal. The concentration of HE4 was noted to increase with age especially in women who were more than 50 years old. We also noted that our proposed reference limit was lower compared to the level given by manufacturer Abbott Architect HE4 kit insert (58.4 vs 70 pmol/L for premenopausal group and 69.0 vs 140 pmol/L in the postmenopausal group). The study also showed a significant difference in HE4 concentrations between ethnic groups (Malays and Indians). The levels of HE4 in Indians appeared higher than in Malays (p<0.05), while no significant differences were noted between the Malays and Chinese ethnic groups. Conclusions: More data are needed to establish a reference interval that will better represent the multiethnic Malaysian population. Probably a larger sampling size of equal representation of the Malay, Chinese, Indians as well as the other native ethnic communities will give us a greater confidence on whether genetics plays a role in reference interval determination.

• Animal Bioscience
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• 제34권7호
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• pp.1123-1133
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• 2021

Objective: Shandong indigenous pig breeds are important Chinese pig resources. Their progressive population decline in recent decades has attracted attention towards their conservation. Conservation genetics of these indigenous breeds are essential for developing a conservation and utilization scheme. Methods: A high-density single nucleotide polymorphism (HD-SNP) chip-based comparative analysis of genetic characteristics was performed for seven Shandong indigenous pig breeds in the context of five Western commercial breeds. Results: The results showed that Shandong indigenous pig breeds varied greatly in genetic diversity, effective population size, inbreeding level, and genetic distance with the Western commercial breeds. Specifically, Laiwu and Dapulian displayed low genetic diversity, and had a genetically distant relationship with the Western commercial breeds (average F statistics [F_ST] value of 0.3226 and 0.2666, respectively). Contrastingly, the other five breeds (Yantai, Licha, Yimeng, Wulain, and Heigai) displayed high genetic diversity within breed and had some extent of mixture pattern with the Western commercial breeds, especially Duroc and Landrace (F_ST values from 0.1043 to 0.2536). Furthermore, intensive gene flow was discovered among the seven Shandong indigenous breeds, particularly Wulian, Licha, and Heigai, as indicated by the large cluster formed in the principal component analysis scatterplot and small population differentiation (average of 0.1253) among them. Conclusion: Our study advances the understanding of genetic characteristics of Shandong indigenous breeds and provides essential information for developing an appropriate conservation and utilization scheme for these breeds.

• 한국환경생태학회지
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• 제36권3호
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• pp.257-276
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• 2022

희귀식물과 특산식물의 보전연구는 국가적 수준에서 식물다양성 유지를 위해 우선되어야 한다. 식물다양성 보전의 필요성에 따라 이들 분류군이 대상이 되는 다양한 연구가 보고되었다. 이러한 시점에서 지금까지의 연구결과를 비교하고 평가할 필요성이 증가하였다. 그리고 수집된 자료를 분석한 결과로부터 국내 희귀식물과 특산식물의 효과적인 보전을 위해 필요한 연구항목과 연구의 방향을 재검토할 필요가 있다. 우리는 희귀식물 및 특산식물이 연구의 대상이 되는 전 세계적인 연구동향을 수집하였다. 그리고 특별히 한국에서의 연구동향을 수집하였다. 희귀 및 특산식물에 대한 연구 분야를 6개의 분야로 구분하였고 그 중 보전생태학의 분야는 '종 특성연구', '개체군 연구' 및 '생물학적 상호작용'의 세부항목으로 구분하였다. 수집된 연구를 종합해 볼 때 보전생태학적 연구는 권역별 그리고 국가별로 큰 차이가 있었다. 보전생태학 연구는 권역별로는 유럽과 북아메리카에서 활발하게 진행되었고 국가별로는 일본과 미국에서 비율이 높았다. 반면에 한국은 기초생태학 연구의 비율이 가장 높았고 보전유전학, 복원생태학 순이었다. 한국에서 보전생태학 연구비율은 세계적인 수준에 비해 낮은 것으로 나타났다. 뿐만 아니라 희귀식물과 특산식물 중에서 환경부 지정 멸종위기식물과 같은 특정 분류군에 대한 연구가 주를 이루었다. 특히, 우리나라에서는 보전유전학 및 복원생태학 연구의 비율이 높았다. 세계적인 연구동향, 특히 보전생태학적 연구를 선도하는 유럽 및 북아메리카와 비교해 볼 때 우리나라에서 희귀 및 특산식물의 효과적인 보전을 위해서는 종 특성에 대한 정보 확보, 개체군 구조와 동태 그리고 식물과 식물, 동물과 식물과의 상호작용을 포함하는 보전생태학적인 연구의 비율을 높이는 접근이 필요함을 제안하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.624-627
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• 2008

An enrichment library of GATA-repeats from genomic DNA was constructed in this study to isolate and characterize microsatellite loci in Tsaiya duck (Anas platyrhynchos). Thirty-three microsatellite markers were developed and used to detect polymorphisms in 30 Tsaiya ducks. A total of 177 alleles were observed and all loci except APT022 were polymorphic. The number of alleles ranged from 2 to 9 with an average of 5.5 per microsatellite locus. The observed and expected heterozygosity of these polymorphic markers ranged from 0.07 to 0.93 with an average number of 0.60 and 0.10 to 0.86 with an average number of 0.61, respectively. Among the polymorphic markers, the observed heterozygosities of 23 loci were higher than 0.50 (69.70%). The polymorphism information content (PIC) in the 32 loci ranged from 0.09 to 0.83 with an average of 0.57. Seven of the 33 duck microsatellite loci had orthologs in the chicken genome, but only APT004 had a similar core repeat to chickens. These microsatellite markers will be useful in constructing a genetic linkage map for the duck and a comparative mapping with the chicken can also provide a valuable tool for studies related to biodiversity and population genetics in this duck species.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.20-20
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• 2003

We described the isolation, characterization and inheritance of twenty-seven microsatellite loci from olive flounder, Paralichthys olivaceus. All loci were found to be polymorphic, and had between five and 22 alleles with observed heterozygosity ranging from 0.161 to 1.0 in 31 individuals examined. Allele segregation patterns of all loci in controlled crosses of flounder were studied to assess the inheritability. Allele of all but 3 loci were segregated according to Mendelian transmission. However, 3 loci had a possibility of scoring errors of heterozygous individuals caused by unreproducible PCR amplification of a particular allele. 24 microsatellite loci are likely to be useful for studies of genome mapping, mating systems and population genetics in this species.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2521-2523
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• 2013

Background: Papillary thyroid cancer or papillary thyroid carcinoma (PTC) is the most common thyroid cancer. The fact that it occasionally occurs in women aged 30-40 years old suggests that genetic alterations are involved its genesis. Recently, activator mutations in BRAF gene have been relatively frequently discovered. Materials and Methods: In this study, we tested 63 DNA samples from PTC patients to identify the V600E mutation frequency in the Ahvaz population. DNA was isolated from formalin fixed paraffin-embedded (FFPE) PTC tumor tissues. Genotyping was performed by PCR-RFLP and confirmed by direct DNA sequencing of a subset of PCR products. PCR-RFLP data were reported as genotype frequencies and percentages. Results: Forty nine out of 63 patients (77.8%) had a mutated heterozygote form while 14 (22.2%) showed normal genotype but none demonstrated a mutant homozygote genotype. The frequency of V600E mutation was significantly high in PTC patients. Conclusions: These findings support involvement of V600E mutations in PTC occurrence in Iran. Assessment of correlations between BRAF V600E mutations and papillary thyroid cancer progression needs to be performed.

• Asian-Australasian Journal of Animal Sciences
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• 제24권5호
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• pp.603-609
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• 2011

Telomeres are special structures at the ends of eukaryotic chromosomes. Vertebrate telomeres consist of tandem repeats of conserved TTAGGG sequence and associated proteins. Birds are interesting models for molecular studies on aging and cellular senescence because of their slow aging rates and longer life spans for their body size. In this longitudinal study, we explored the possibility of using telomeres as an age-marker to predict age in Single Comb White Leghorn layer chickens. We quantified the relative amount of telomeric DNA in isolated peripheral blood lymphocytes by the Quantitative Fluorescence in situ Hybridization technique on interphase nuclei (IQ FISH) using telomere-specific DNA probes. We found that the amount of telomeric DNA (ATD) reduced significantly with an increase in chronological age of the chicken. Especially, the telomere shortening rates are greatly increased in growing individuals compared to laying and old-aged individuals. Therefore, using the ATD values obtained by IQ FISH we established the possibility of age prediction in chickens based on the telomere theory of aging. By regression analysis of the ATD values at each age interval, we formulated an equation to predict the age of chickens. In conclusion, the telomeric DNA values by IQ FISH analyses can be used as an effective age-marker in predicting the chronological age of chickens. The study has implications in the breeding and population genetics of poultry, especially the reproductive potential.

• 한국자원식물학회지
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• 제32권6호
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• pp.644-668
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• 2019

Dysphania ambrosioides (L.) Mosyakin & Clemants which belongs to Chenopodiaceae/Amaranthaceae sensu in APG system has been known as a useful plant in various fields as well as an invasive species spreading all over the world. To understand its phylogenetic relationship with neighbour species, we completed chloroplast genome of D. ambrosioides collected in Korea. Its length is 151,689 bp consisting of four sub-regions: 83,421 bp of large single copy (LSC) and 18,062 bp of small single copy (SSC) regions are separated by 25,103 bp of inverted repeat (IR) regions. 128 genes (84 protein-coding genes, eight rRNAs, and 36 tRNAs) were annotated. The overall GC content of the chloroplast genome is 36.9% and those in the LSC, SSC and IR regions are 34.9%, 30.3%, and 42.7%, respectively. Distribution of simple sequence repeats are similar to those of the other two Dysphania chloroplasts; however, different features can be utilized for population genetics. Nucleotide diversity of Dysphania chloroplast genomes 18 genes including two ribosomal RNAs contains high nucleotide diversity peaks, which may be genus or species-specific manner. Phylogenetic tree presents that D. ambrosioides occupied a basal position in genus Dysphania and phylogenetic relation of tribe level is presented clearly with complete chloroplast genomes.