• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.6
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• pp.446-451
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• 2008

The growth of Porphyridium cruentum and its porphyran polysaccharide production were measured as functions of light intensity, temperature, light quality (fluorescent, blue, red, and green) and nitrate concentration. The optimum light intensity, temperature, and nitrate concentration for the growth of Porphyridium cruentum and for its polysaccharide production were 1,400 lx, $25^{\circ}C$, and 0.03%, respectively. The maximum cell concentration and polysaccharide content under the optimum conditions were 1.95 and 0.23 mg/mL, respectively. Light quality did not influence growth or polysaccharide production. The best results for growth and polysaccharide production were obtained using fluorescent light.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.149-152
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• 2003

This experiment was carried out to obtain the optimal liquid culture conditions for the mycelial growth and the polysaccharide production of Tricholoma matsutake. For the mycelial growth and polysaccharide production, the synthetic medium was optimized with containing glucose 40 g/L, yeast extract 30 g/L, $KH_2PO_4$ 1.5 g/L and $MgSO_4.7H_2O$ 1 g/L. The effects of agitation and aeration were investigated for the cell growth and the polysaccharide production in batch culture. The biomass and polysaccharide concentrations were 21.87 g/L at 150 rpm and 8.86 g/L at 300 rpm, respectively. And the biomass concentration and the polysaccharide production were 20.85 g/L at 0.5 vvm and 8.83 g/L at 1.5 vvm, respectively.

• KSBB Journal
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• v.22 no.1
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• pp.1-6
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• 2007

The optimum liquid culture conditions were investigated for cell growth and polysaccharide production from liquid culture of Lentinus edodes. In flask culture, the optimal medium compositions for the polysaccharide production contained glucose 60 g/L, yeast extract 10 g/L, $KH_2PO_4$ 2.0 g/L, and $MgSO_4{\cdot}7H_2O$ 1.0 g/L. The maximum mycelial growth and polysaccharide production were 11.01 g/L and 1.64 g/L, respectively. In bioreactor, through the variation of aeration in order to increase mycelial growth and polysaccharide production, the maximum mycelial growth and polysaccharide production were 55.9 g/L at 8th day and 7.34 g/L at 7th day of cultivation with 1.5 vvm, respectively.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.368-375
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• 2005

Batch cultures were carried out to optimize the exo-polysaccharide production by liquid cultures of Pleurotus ferulae. Among the various carbon sources, when $5\%$ of glucose was used, the maximum mycelial growth and exo-polysaccharide concentration reached were 8.78 g/l and 3.59 g/l, respectively. Yeast extract and polypeptone were identified as the most suitable nitrogen sources. In particular, when a mixture of $1\%$ of polypeptone and $0.8\%$ of yeast extract was used, 9.52 g/l of mycelial growth and 4.09 g/l of exo-polysaccharide were obtained. In the case of mineral sources, K$_2$HPO$_4$ and MgSO$_4$$\codt$7H$_2$0 were found to be the best mineral sources for mycelial growth and exo-polysaccharide production. Under the optimized culture conditions, the agitation speed and aeration were investigated for mycelial growth and exo­polysaccharide production in a jar fermentor. The maximum mycelial growth and exo-polysaccharide concentration at 1.5 vvm and 200 rpm obtained were 13.2 g/l and 4.95 g/l, respectively, after 10 days of culture, which were $76\%$ and $79\%$ higher than those of the basal medium. The specific growth rate was decreased with the increase of mycelial growth. However, the specific production rate of the exo-polysaccharide was proportionally increased with the specific growth rate. The proposed model profiles showed good agreement with the experimental results for the mycelial growth and exo-polysaccharide production. The specific production rate using the optimized medium was higher than that of basal medium.

• Mycobiology
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• v.31 no.4
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• pp.205-208
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• 2003

The production of polysaccharide according to various developmental stages(mycelium growth, primordium appearance, and fruiting-body formation) in the edible mushroom Grifola frondosa was studied. The cap of the mature mushroom showed the highest amount of polysacchride. Mycelial growth and polysaccharide synthesis were optimal at pH 5 and $20^{\circ}C$. Polysaccharide synthesis was maximal after 12 days of cultivation, whereas maximum mycelial growth was shown after 18 days. Mannose, cellobiose and starch increased the level of polysaccharide as well as growth in submerged culture. Glucose and sucrose appeared to be good substrates for fruiting of Grifola frondosa.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.231-234
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• 2000

The polysaccharide has been known to have an antitumor activity, which were extracted from the fruiting bodies, mycelia, and culture broths of Agaricus blazei. For the cell growth and the polysaccharide production, the optimal medium contained 8% glucose, 2% yeast extract, 0.1% $MgSO_4{\cdot}7H_2O$, 0.2% $KH_2PO_4$, 0.2% $CaCl_2{\cdot}2H_2O$ and 0.2% $MnSO_4{\cdot}5H_2O$. When 0.2% of glutamic acid was added at 4day, the cell concentration was 13.5 g/L and the polysaccharide production was 9.9 g/L, respectively.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.289-293
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• 2003

Schizophyllum commune belongs to Basidiomycetes is able to produce an extracellular polysaccharide, beta-glucan, which has been well known as an effective immune stimulator. In other to obtain the optimum conditions for the polysaccharide production, Schizophyllum commune was cultivated in different cultivation media containing various carbon and nitrogen sources. For the cell growth and the polysaccharide production, the optimum synthetic medium was developed with YMP medium an a basal midium. The flask culture conditions for the polysaccharide production were $27^{\circ}C$ and 150rpm with the initial pH 5.5.

• Microbiology and Biotechnology Letters
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• v.26 no.2
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• pp.130-136
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• 1998

An Immunostimulating polysaccharide was produced from the suspension culture of Angelica gigas H4, plant cells. In order to enhance the polysaccharide production by the A. gigas cell culture, medium composition and physical conditions were optimized. Schenk and Hildebrandt (SH) medium was selected as an optimal basal medium for the growth of A. gigas. The maximum cell and polysaccharide concentration obtained in SH medium were 15.8 g DCW/l and 0.85 g polysaccharide/l, respectively, at $25^{\circ}C$ under dark condition. For the enhanced polysaccharide production, a polysaccharide production medium (PPM) was established by modifying Gamborg B5 medium with optimized carbon sources, growth regulators, organic and inorganic elements. Optimal initial pH and temperature were 6.0-6.6 and $20^{\circ}C$, respectively, and the dark condition was better than the light condition. The maximum polysaccharide concentration of 1.5 g/l could be obtained through the optimization of the medium composition and physical conditions.

• Korean Journal of Food Science and Technology
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• v.19 no.5
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• pp.414-419
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• 1987

Cultural conditions for the polysaccharide production by Pseudomonas elodea ATCC 31461, a thermogellable polysaccharide producer, were investigated. Optimum condition for polysaccharide production were: temperature; $30^{\circ}C$, pH; 6.5, glucose; 25 g/L. nitrogen source; peptone, C/N ratio; 5.0. Maximum production and yield at this condition were ca. 32 g/L and 128%, viscosity of 1% polysaccharide solution was 837 mPas at 35/sec.. Consistency index and flow behaviour index were 2570 mPas and 0.66

• KSBB Journal
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• v.10 no.2
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• pp.176-182
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• 1995

The environmental and physiological factors affecting the production of exopolysaccharide (Methylan) and Poly-${\beta}$-hydroxybutyrate(PHB) by Methylobacterium organophilum were investigated. The maximum PHB content was obtained at $38^{\circ}C$ whereas maximum polysaccharide concentration was $3.54g/\ell$ at $30^{\circ}C$. Optimum pH was pH 7-8 for PHB production and pH 6-7 for polysaccharide production, respectively. Under the condition of $Mo^{2+}, Mg^{2+} or Mn^{2+}$ limitation with nitrogenlimitation, the PHB accumulation was increased, whereas the polysaccharide production was decreased as compared with that of solenitrogenlimitation. Under the condition of sole K+ limitation, cell growth was significantly inhibited and no polysaccharide was produced. However, the PHB content was as high as 60% of dry cell weight. Effect of C/N ratios (methanol/ammonium) in the feeding solution was examined for the simultaneous production of polysaccharide and PHB. The higher ratio of C/N showed the lower cell growth, higher content of PHB in cells, and higher yield of polysaccharide.