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Effect of Culture Conditions on Production of Polysaccharides and Growth Rate of Porphyridium cruentum

Porphyridium cruentum의 성장 및 당질 생산에 미치는 배양 조건의 영향

  • Joo, Dong-Sik (Dept. of Foodservice Industry, Hanzhong University) ;
  • Choi, Soon-Yeong (East Coastal Marine Bioresources Research Center, Kangnung National University)
  • 주동식 (한중대학교 외식산업학과) ;
  • 조순영 (강릉대학교 동해안해양생물자원연구센터)
  • Published : 2008.12.31

Abstract

The growth of Porphyridium cruentum and its porphyran polysaccharide production were measured as functions of light intensity, temperature, light quality (fluorescent, blue, red, and green) and nitrate concentration. The optimum light intensity, temperature, and nitrate concentration for the growth of Porphyridium cruentum and for its polysaccharide production were 1,400 lx, $25^{\circ}C$, and 0.03%, respectively. The maximum cell concentration and polysaccharide content under the optimum conditions were 1.95 and 0.23 mg/mL, respectively. Light quality did not influence growth or polysaccharide production. The best results for growth and polysaccharide production were obtained using fluorescent light.

Keywords

References

  1. Arad, S.G., G. Keristovesky, B. Simon, Z. Barak and S. Geresh. 1993. Biodegradation of the sulfated poly- saccharide of Porphyridium by soil bacteria. Phytochem., 32, 287-290 https://doi.org/10.1016/S0031-9422(00)94982-3
  2. Arad, S.G., O. Friedman and A. Rotem. 1988. Effect of nitrogen on polysaccharide production in a Por- phyridium sp. App. Environ. Microbiol., 54, 2411- 2414
  3. Arnaud, M.F., G. Roland and P. Jérémy. 2003. Benefits and limitations of modeling for optimization of Porphyridium cruentum cultures in an annular photobioreactor. J. Biotech., 103, 153-163 https://doi.org/10.1016/S0168-1656(03)00100-7
  4. Bermejo Roman, R., J.M. Alvarez-Pez, F.G. Fernandez and E. Molina Grima. 2002. Recovery of pure B-phycoerythrin from the microalga Porphyridium cruentum. J. Biotech., 93, 73-85 https://doi.org/10.1016/S0168-1656(01)00385-6
  5. Cohen, Z. and S. Cohen. 1991. Preparation of eicosapen- taenoic acid (EPA) concentrate from Porphyridium cruentum. JAOCS, 68, 16-19 https://doi.org/10.1007/BF02660301
  6. Dubois, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers and F. Smith. 1958. Colorimetric method for determina- tion of sugar and related substances. Aal. Chem., 28, 350-356
  7. Geresh, S., N. Lupescu and S. Arad. 1992. Fractionation and partial chracterization of the sulfated poly- saccharide of Porphyridium sp. Phytochem., 31, 4184-4186
  8. Jones, R.E., H.L. Speer and W. Kury. 1963. Studies on the growth of the red alga Porphyridium cruentum. Physiol. Plant, 16, 636-643 https://doi.org/10.1111/j.1399-3054.1963.tb08342.x
  9. Joo, D.S. and S.Y. Cho. 2007. Physiochemical properties of carrageenan hydrolysates by organic acids. J. Korean Soc. Food Sci. Nutr., 36, 262-268 https://doi.org/10.3746/jkfn.2007.36.3.262
  10. Joo, D.S., M.G. Cho, R. Buchholz and E.H. Lee. 1998. Growth and fatty acid composition with growth conditions for Spirulina platensis. J. Korean Fish. Soc., 31, 409-416
  11. Jorge, B. and R.P. Marco. 2004. Bioprocess intensifica- tion:a potential aqueous two-phase for the primary recovery of B-phycoerythrin from Porphyridium cruentum. J. Chrom., 807, 33-38 https://doi.org/10.1016/j.jchromb.2004.01.028
  12. Lupescu, N., S. Arad, S. Geresh, M.A. Bernstein and R. Glaser. 1991. Structure of some sulfated sugars isolated after acid hydrolysis of the extracellular polysaccharide of Porphyridium sp., a unicellular red alga. Carbohy. Res., 210, 349-352 https://doi.org/10.1016/0008-6215(91)80136-B
  13. Osumi, Y., M. Kawai, H. Amano and H. Noda. 1997. Purification and characterization of porphyran- decomposing enzymes from Arthrobacter sp. S-22. Nippon Suisan Gakkaishi, 63, 757-764 https://doi.org/10.2331/suisan.63.757
  14. Percival, E. and R.A.J. Foyle. 1989. The extracellular polysaccharides of Porphyridium cruentum and Porphyridium aeruginium. Carbohy. Res., 72, 165- 172 https://doi.org/10.1016/S0008-6215(00)83932-4
  15. ichmond, A. 1986. Microalgae of economic potential. In Handbook of Microalgal Mass Culture. Richmond, A., ed. CRC Press, Florida, USA., 199-243
  16. You, T. and S.M. Barnett. 2004. Effect of light quality on production of extracellular polysaccharides and growth rate of Porphyridium cruentum. Biochem. Eng. J., 19, 251-258 https://doi.org/10.1016/j.bej.2004.02.004

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