• Biomedical Science Letters
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• v.25 no.4
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• pp.398-406
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• 2019

Cancer immunotherapy using gene-modified tumor cells is safe and customized cancer treatment method. In this study, we made gene-modified tumor cells by transferring costimulatory molecules, 4-1BBL and OX40L, into tumor cells using lentivirus vector, and identified anti-cancer effect of gene-modified tumor cells in CT26 mouse colorectal tumor model. We construct pLVX-puro-4-1BBL, -OX40L vector for lentivirus production and optimized the transfection efficiency and transduction efficiency. The transfection efficiency is maximal at DNA:cationic polymer = 1:0.5 and DNA 2 ㎍ for lentivirus production. Then, the lentiviral including 4-1BBL and OX40L was used to deliver CT26 mouse tumor cells to establish optimal delivery conditions according to the amount of virus. The transduction efficiency is maximal at 500 μL volume of lentiviral stock without change in cell shape or growth rate. CT26-4-1BBL, CT26-OX40L significantly inhibited the tumor growth compare with CT26-WT or CT26-β-gal cell line. These data showed the possibility the use of genetically modified tumor cells with costimulatory molecule as cancer immunotherapy agent.

• Structural Engineering and Mechanics
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• v.39 no.2
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• pp.207-221
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• 2011

This paper aims to study the behavior of short reinforced concrete columns confined with external glass Fiber Reinforced Polymers (GFRP) sheets under eccentric loads. The experimental part of the study was achieved by testing 9 specimens under eccentric compression. Three eccentricity ratios corresponding to e/t = 0, 0.10, 0.50 in one direction of the column were used. Specimens were divided into three groups. The first group was the control one without confinement. The second group was fully wrapped with GFRP laminates before loading. The third group was wrapped under loading after reaching 75% of failure loads of the control specimens. The third group was investigated in order to represent the practical case of strengthening a loaded column with FRP laminates. All specimens were loaded until failure. The results show that GFRP laminates enhances both failure load and ductility response of eccentrically loaded column. Moreover, the study also illustrates the effect of confinement on the first crack load, lateral deformation, strain in reinforcement and failure pattern. Based on the analysis of the experimental results, a simple model has been proposed to predict the improvement of load carrying capacity under different eccentricity ratios. The predicted equation takes into consideration the eccentricity to cross section depth ratio, the ultimate strength of GFRP, the thickness of wrapping laminate, and the time of wrapping (before loading and under loading). A good correlation was obtained between experimental and analytical results.

• YAKHAK HOEJI
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• v.44 no.4
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• pp.293-299
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• 2000

Biodegradable microspheres made from poly-lactide-co-glycolide polymers have been considered as a new delivery system for single-dose vaccine. Purified tetanus toxoid (TT) was encapsulated in poly-lactide(PLA) and poly-lactide-co-glycolide (PLGA) microparticles using a solvent evaporation method in a multiple emulsion system (water-in oil-in water). The morphology of 77-loaded microparticles was spherical and the suface of them was smooth. The particle size was in a range of 2-10. Protein loading efficiency was 68-97.8%. PLGA (85:15) microparticle showed the highest efficiency. Protein release pattern was influenced by polymer molecular weight and composition. The release rate of PLA(Mw 100,000) microsphere was higher than any other microspheres. In consequence of the hydrolysis of PLGA(50:50) microspheres, environmental pH decreased from 7.4 to 5.0. The PLA, PLGA (75:25) and PLGA (85:15) microshperes showed no significant pH change. The antigenicity or n in microshperes was assayed by indirect sandwich ELISA using equine polyclonal tetanus antitoxin for capture antibody and human polyclonal tetanus antitoxin for primary antibody. The antigenicity of TT in PLA (Mw 100,000), PLGA(50:50, Mw 100,000) and PLGA (75:25, Mw 73,300) after 30 days incubation showed 54, 40.9 and 76.7%, respectively.

• Journal of electromagnetic engineering and science
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• v.14 no.3
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• pp.293-298
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• 2014

Large spaceborne antennas should be lightweight, a factor related to the development costs of launch vehicles. In order to overcome this drawback, a feasibility study of a new carbon fiber reinforced polymer (CFRP) named M55J/RS3 is carried out for a synthetic aperture radar (SAR) reflector antenna. In particular, the high resolution of detected images is taken into consideration. To validate the electrical performance, a test of the CFRP specimen is fabricated, and the transmission/reflection coefficients are measured using a standard X-band waveguide. Finally, the effective complex permittivity and effective electrical conductivity are derived from the obtained measured data. By applying the derived conductivity to the simulation of the radiation pattern, antenna gain, and beamwidth-instead of relying on the assumption of a perfect electric conductor-variations in electrical performance are also investigated and discussed.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.13 no.4
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• pp.68-74
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• 1999

Ths paper does acoustic detection of partial discharge using acoustic sensor in polymer. Time sequential rreasurement of acoustic emission characteristic obtained acoustic sensor deal with statistics process. and 5 characteristic quantities were introduced into this paper. Resulting fann analysis of $\psi$-AEA-n pattern (phase-acoustic emission amplitude-pulse number) and AE quantities ,it can know useful statistics quantities that AE average inception amplitude TEX>$(\overline{AEA_{inc}})$ and AE average maximum amplitude TEX>$(\overline{AEA_{max}})$ make diagnosis of the middle stage of deterioration, AE pulse number and AE average maximum phase $(\overline{\theta{max}})$ make diagnosis of the last stage of deterioration. it obtained that these AE quantities are useful for dias,mosis deterioration form experiment results.esults.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2011.11a
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• pp.44-44
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• 2011

본 연구에서는 직물조직의 분석과정을 자동화할 수 있는 전용 하드웨어 및 소프트웨어를 개발하였다. 직물조직의 분석은 직물 설계 및 품질관리 단계에서 매우 중요한 공정이나 현재까지도 확대경과 분해침에 의존하여 수작업으로 이루어지고 있는 것이 실정이라 자동화가 절실히 요구되는 분야라고 할 수 있다. 최근 컴퓨터 기술의 발달로 섬유산업 분야에서도 자동화 관련 연구가 많이 이루어지고 있으며 직물 분석 과정에 대한 연구도 여러 차례 시도된 바 있다. 여기에는 주로 디지털 영상으로부터 특징을 찾아내고 분석하는 이미지 프로세싱 기법이 쓰였는데 이는 재현성, 정확성, 속도 등에서 육안에 의한 방법과는 비교할 수 없는 장점을 가지고 있다. 그러나 기존의 연구들은 카메라의 한계로 인해 주로 저해상도의 이미지를 가지고 작업을 하거나, 이미지 프로세싱 또는 인공 신경망을 단독으로 적용하는 등의 한계를 가지고 있었다. 본 연구에서는 이러한 문제점을 극복하기 위해 초고해상도 직물 이미지를 획득한 뒤 이로부터 직물의 교차점을 인식하는데 필요한 파라미터를 추출하고 이를 인공 신경망에 학습시켜 직물 설계에 필요한 조직도를 생성하는 시스템을 개발하고자 하였다. 이를 위해 먼저 컴퓨터로 컨트롤이 가능한 직물 분석 전용 초정밀 XY 스테이지의 하드웨어를 설계 제작 하였으며 각종 이미지 분석 및 하드웨어 컨트롤에 필요한 전용 소프트웨어를 개발하였으며 그 결과 각종 직물의 조직을 매우 정확히 인식할 수 있게 되었다. 향후 이미지 획득 과정의 보완, 새로운 파라미터의 정의 및 신경망의 반복 학습을 통해 본 시스템이 보다 보완된다면 직물 분석 작업의 자동화를 통한 제품 개발과 생산에 소요되는 시간을 단축 및 품질 관리 공정의 자동화에 기여할 수 있을 것으로 기대된다.

• Proceedings of the KIEE Conference
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• 2007.11a
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• pp.100-101
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• 2007

Polymer thick film capacitors were successfully fabricated by using ink-jet printing and screen printing technology. First, a bottom electrode was patterned by ink-jet printing of a nano-sized silver ink. Next, a dielectric layer was formed by the screen printing, then a top electrode was pattern by ink-jet printing of a nano-sized silver ink. The printed area of the dielectric layers were changed into $2{\times}2m^2$and $4{\times}2m^2$, and also the area of the electrodes were patterned with $1{\times}1mm^2$ and $1{\times}3mm^2$. The thickness of the printed dielectric layer was ranged from 1.1 to $1.4{\mu}m$. The analysis of capacitances verified that the capacitances was proportional to the area of the printed electrode. The capacitances of the fabricated capacitors resulted in one third of the calculated capacitances.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.24 no.10
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• pp.790-793
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• 2011

In this study, the surface modification for a silicon(Si) mold using $CHF_3$ inductively coupled plasma(ICP). The conditions under that plasma was treated a input ICP power 600 W, an operating gas pressure of 10 mTorr and plasma exposure time of 30 sec. The Si mold surface became hydrophobic after plasma treatment in order to $CF_x$(X= 1,2,3) polymer. However, as the de-molding process repeated, it was investigated that the contact angle of Si surface was decreased. So, we attempted to investigate the degradation mechanism of the accurate pattern transfer with increasing the count of the de-molding process using scanning electron microscope (SEM), contact angle, and x-ray photoelectron spectroscopy (XPS) analysis of Si mold surface.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2005.10a
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• pp.698-701
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• 2005

We have investigated experimentally a nano patterning using electron beam lithography for the nickel stamper fabrication. Recently, DVD and Blu-ray disk(BD) have nano-scale patterns in order to increase the storage density. Specially, BD has 100nm-scale patterns which are generally fabricated by electron beam lithography. In this paper, we found optimum condition of electron-beam lithography for 100nm-scale patterning. We controlled various conditions of EHP(acceleration voltage), beam current, dose and aperture size in order to obtain optimum conditions. We used 100nm-thick PMMA layer on a silicon wafer as photoresist. We found that EHP was the most dominant factor in electron-beam lithography.

• Computers and Concrete
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• v.16 no.2
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• pp.245-260
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• 2015

In this study, nominal moment-axial load interaction diagrams, moment-curvature relationships, and ductility of rectangular hybrid beam-column concrete sections are analyzed using the modified Hognestad concrete model. The hybrid columns are primarily reinforced with steel bars with additional Glass Fiber Reinforced Polymer (GFRP) control bars. Parameters investigated include amount, pattern, location, and material properties of concrete, steel, and GFRP. The study was implemented using a user defined comprehensive $MATLAB^{(R)}$ simulation model to find an efficient hybrid section design maximizing strength and ductility. Generating lower bond stresses than steel bars at the concrete interface, auxiliary GFRP bars minimize damage in the concrete core of beam-column sections. Their usage prevents excessive yielding of the core longitudinal bars during frequent moderate cyclic deformations, which leads to significant damage in the foundations of bridges or beam-column spliced sections where repair is difficult and expensive. Analytical results from this study shows that hybrid steel-GFRP composite concrete sections where GFRP is used as auxiliary bars show adequate ductility with a significant increase in strength. Results also compare different design parameters reaching a number of design recommendations for the proposed hybrid section.