• Journal of Microbiology and Biotechnology
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• 제22권1호
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• pp.58-68
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• 2012

An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwha Island in the Korean West Sea. A prominent effect on BHAP production was obtained by adding 2% maltose, 1% sodium citrate, 0.8% NaCl, and 0.6% sodium carbonate to the culturing medium. The optimal medium for BHAP production contained (g/l) SBM, 15; casein, 10; $K_2HPO_4$, 2; $KH_2PO_4$, 2; maltose, 20; sodium citrate, 10; $MgSO_4$, 0.06; NaCl, 8; and $Na_2CO_3$, 6. A protease yield of approximately 56,000 U/ml was achieved using the optimized medium, which is an increase of approximately 5.5-fold compared with the previous optimization (10,050 U/ml). The BHAP was homogenously purified 34-fold with an overall recovery of 34% and a specific activity of 223,090 U/mg protein using adsorption with Diaion HPA75, hydrophobic interaction chromatography (HIC) on Phenyl-Sepharose, and ion-exchange chromatography on a DEAE- and CM-Sepharose column. The purified BHAP was determined a homogeneous by SDS-PAGE, with an apparent molecular mass of 28 kDa, and it showed extreme stability towards organic solvents, SDS, and oxidizing agents. The $K_m$ and $k_{cat}$ values were 78.7 ${\mu}M$ and $217.4s^{-1}$ for N-succinyl-Ala-Ala-Pro-Phe-pNA at $37^{\circ}C$ and pH 9, respectively. The inhibition profile exhibited by PMSF suggested that the protease from B. horikoshii belongs to the family of serine proteases. The BHAP, which showed high stability against SDS and $H_2O_2$, has significance for industrial application, such as additives in detergent and feed industries.

• 한국수산과학회지
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• 제13권1호
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• pp.33-44
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• 1980

부착 생물에 관한 연구는 선박, 해수 이용시설, 수산 양식업 둥의 방제대책과 관련하여 부착 생물의 종류, 부착 량, 성장도 및 계절적 변화 둥의 연구가 중요한 문제하고 생각한다. 특히 양식시설이 밀집한 진해만 해역의 굴 양식 시설에 부착하는 대부분의 생물들이 Ascidicea, Crustacea, Cirripedia, Bivalvia, Bryozoa, Polychaeta, marine algae 등이며, 이들 가운데 피해가 심한 다모환충류에 관하여 분류학적인 연구를 시도하였던 바 수하연의 양식 굴 사이에서 많은 양의 갯지렁이류를 얻었기에 보고 한다. 수하연에 서식하는 갯지렁이류는 제2부착성(Kazihara, 1964)의 갯지렁이류로서 왕관 모양의 큰 아가미 혹은 몸앞 부분에 실모양의 감각 촉수를 가지고 굴에 피해를 입히는 짧은수술갯지렁이 Loimia medusa, 미끈꽃갯지렁이 Myxicola infundibulum와 제1부착성으로서 굴 패각, 양식 시설 등에 석회질의 집을 형성시켜 피해를 입히는 우산관덮개꽃갯지렁이 Hydroides ezoensis, 둥근구멍환덮개꽃갯지렁이 Dexiospira alveolatus 등의 7과 12속 13종을 보고한다. 이 가운데 새날개갯지렁이 Chaetopterus variopedatus와 둥근구멍관덮개꽃갯지렁이 Dexiospira alveolatus는 한국 미기록종이다.

• Animal Systematics, Evolution and Diversity
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• 제26권3호
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• pp.279-293
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• 2010

Three new species of Hemicyclops, H. parilis, H. nasutus and H. membranatus are described from Korean coasts. Hemicyclops parilis n. sp. is closely similar to H. gomsoensis Ho and Kim, but distinguishable from the latter species by having the different shape of genital double somite and spermatophore. Hemicyclops nasutus n. sp. is distinguishable from congeners by having five setae on the first segment of antennule and the ratio 4.26 : 1 of the length to width of caudal ramus. Hemicyclops membranatus n. sp. is characterized by the possession of the membranous fringe along margins of cephalothorax and a claw on the third antennal segment.

• Animal Systematics, Evolution and Diversity
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• 제32권4호
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• pp.266-271
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• 2016

Two new records of scolecid polychaetes, Praxillella pacifica Berkeley, 1929 and Naineris dendritica (Kinberg, 1867), collected from Korean waters are reported here with descriptions and illustrations. Praxillella pacifica can be distinguished from its congeners by the number of asetigerous segments on the posterior region of the body and features of the anal cone and neuropodial spines on setigers 2 and 3. Naineris dendritica is distinguishable from its relatives by the morphology of thoracic neuropodial lobes and the thoracic uncini at the inferior position. In the present paper, keys to Praxillella and Naineris species from Korean waters were also provided.

• Animal Systematics, Evolution and Diversity
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• nspc3호
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• pp.29-38
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• 1992

본 연구는 1980년 7월부터 1991년 11월까지 서해안의 15개 지소에서 채집된 염주발갯지렁이과(Syllidae) 표본들을 동정 분류한 것으로써, 그 결과 5속 8종이 얻어졌다. 이들 중, Exogone gemmifera, Syllis amica 2종은 한국 미기록종이며, 이들에 관하여는 기재하고 그림을 그렸다.

• 자연과학논문집
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• 제10권1호
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• pp.49-56
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• 1998

1995년 10월부터 1996년 9월까지 인천 화수 부두 주변 해역의 저서생물상을 조사하였다. 다모류가 모든 정점에서 4계절 모두 우점하였다. 그 다음으로 연체동물과 갑각류가 많이 출현하였으며 극피동물은 닻해삼 1종(Protankyra bidentata)만이 출현하였다. 정점 1에서는 오염지표종으로 알려진 Capitella capitata가 적은 수나마 출현하여 다른 정점에 비하여 오염이 다소 진행된 것으로 생각되며, 정점2와 3에서는 Heteromastus sp.가 다량 출현하여, 유기물 유입이 많은 곳으로 추정된다.

• Animal Systematics, Evolution and Diversity
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• 제36권3호
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• pp.268-273
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• 2020

A spionid polychaete, Boccardiella hamata (Webster, 1879) has been found from mud in crevices between the shells of oysters and adherent substrates in South Korea. The sequences of mitochondrial DNA (mtDNA) cytochrome c oxidase subunit 1 (CO1), 16S ribosomal DNA (16S), and the nuclear 18S ribosomal DNA (18S) from Korean individuals of Boccardiella hamata were determined in the present study. The molecular analysis based on the 18S rRNA gene sequences showed clear separation among the spionid polychaete species, and the sequences of Korean and Japanese individuals are completely identical. The morphological diagnosis and photographs of B. hamata are also provided.

• Animal Systematics, Evolution and Diversity
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• 제36권3호
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• pp.258-263
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• 2020

The aim of this study is to report monotypic species, Nonparahalosydna pleiolepis(Marenzeller, 1879) for the first time from Korean waters with its DNA barcoding data. We collected individuals of the species from the subtidal zone of southern coast of Korea through scuba diving. To estimate DNA barcoding gap, the pairwise genetic distances were calculated between N. pleiolepis and its congeners (Halosydna brevisetosa Kinberg, 1856 and Lepidonotus squamatus (Linnaeus, 1758)) based on the cytochrome c oxidase subunit I gene (COI). Inter-specific genetic distances ranged from 18.7% to 24.6%, while intra-specific genetic distance within N. pleiolepis ranged from 0.3% to 0.5%. The maximum intra-specific genetic distance among the three species was 1.4%. The morphological diagnosis of N. pleiolepis with a taxonomic note on the species were also provided.

• Animal Systematics, Evolution and Diversity
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• 제35권3호
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• pp.156-159
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• 2019

The phyllodicd polychaete species, Nereiphylla hera Kato and Mawatari, 1999 is reported from the intertidal habitats of the eastern coast of South Korea. We determined the DNA barcoding region of the mitochondrial cytochrome c oxidase subunit I (COI) of N. hera and compared nucleotide variation with its congeners. The intra-specific genetic distance between the three COI sequences of N. hera was ranged from 0 to 0.4%. The inter-specific distances between N. hera and other Nereiphylla species ranged from 18.8 to 22.3%. In this study, we reported the first COI barcodes of N. hera with the morphologcial diagnosis and the photographs. These results would be helpful to understand taxonomy of Nereiphylla.

• Animal Systematics, Evolution and Diversity
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• 제38권3호
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• pp.144-147
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• 2022

The polychaete Scolelepis (Scolelepis) sagittaria was originally described from Japanese waters and subsequently reported from Korean waters. In this study, we determined for the first time the mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA (16S rDNA), and nuclear 18S ribosomal DNA (18S rDNA) sequences of Korean specimens of S. (S.) sagittaria. We also assessed intraspecific variation in the shape of the prostomium of this species based on an examination of 247 individuals. All materials were collected from intertidal sandy beaches of the Korea Strait. The molecular data and morphological observations reported herein will contribute to gaining a better understanding of the taxonomic relationships among members of the genus Scolelepis.