• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.339-346
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• 2007

Pollen germination viability is an essential factor to produce seeds from pollination and fertilization, which are required to maintain plant generation. In this study, we tried to identify the effect of boric acid on pollen germination and tube grouch in non-transgenic and transgenic plants expressing monoclonal antibodies (anti-colorectal cancer mAb CO17-1A, anti-breast cancer mAb BR55, and anti-rabies virus mAb57). The pollen of non-transgenic plant was treated with different concentration of boric acid (0, 5, 10, 15, 20, $40{\mu}g/mL$) in germination buffer to investigate its effect on in vitro pollen germination. At $20{\mu}g/mL$ of boric acid, tile pollen germination rate was the highest (49.5%) compared to other concentrations. In general, the germination rate significantly increased 3-10 folds in boric acid ($20{\mu}g/mL$) treated group in non-transgenic and transgenic plants. Also, the pollen tube length increased in boric acid ($20{\mu}g/mL$) treated groups. In the treated group, the pollen tube length increased until 3 h boric acid treatment and decreased after the 3 h, indicating that the 3 h is the most appropriate incubation time period. Western blot analysis showed that the mAb transgene expression was more stable in leaf than pollen in transgenic plants. This study suggested that $20{\mu}g/mL$ of boric acid is ideal concentration to induce in vitro pollen germination of transgenic plants expressing therapeutic monoclonal antibodies, indicating stable pollination and fertilization in transgenic plants.

• Korean Journal of Food Science and Technology
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• v.29 no.5
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• pp.869-875
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• 1997

The chemical components of Korean pollens and pollen extracts were analysed to provide fundamental data for pollen processing and products development. The contents of crude protein, crude lipid, crude ash, crude fiber and carbohydrate of pollens were $14.0{\sim}22.3%,\;3.0{\sim}8.8%,\;2.2{\sim}3.3%,\;0{\sim}37.5%,\;and\;31.9{\sim}64.4%$ respectively, showing the specially high crude fiber content (37.5%) in Pine pollen. Almost all the carbohydrate, lipid, protein and ash components except fiber were extracted by water and n-hexane. Free sugars identified in water extracts $(10\;^{\circ}Bx)$ of pollens were fructose and glucose as major component and maltose as minor component. The proportions of three free sugars to total soluble solid content of Mixed, Acorn, and Pine water extracts were 73.6%, 85.4% and 47.7% respectively. Potassium and phosphorus content in pollens and pollen water extracts were high, but Ca, Na, Mg, Fe and Zn were not major mineral components. The essential amino acids such as leucine, phenylalanine, methionine, lysine, valine, isoleucine and threonine were contained richly in pollens and those were almost completely extracted by water. Vitamin $B_1$ and C were not detected, but only small amount of vitamin $B_2$ was detected in the pollens and pollen extracts tested. The essential fatty acids such as linoleic acid and linolenic acid were contained richly in pollens. The proportion of unsaturated fatty acids to total fatty acid content in Mixed, Acorn and Darae pollen except Pine was higher than 50%.

• Korean Journal of Environmental Biology
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• v.24 no.4
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• pp.368-371
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• 2006

The complex analysis of male reproductive system of vines and fruit-tyees growing in zone of the Armenian Nuclear Power Plant (ANPP) is realized. The obtained data demonstrate that the homogeneous pollen with high fertility and optimal morphometric parameters in all variants is formed. The obtained results demonstrate that there is no one-sided effect of ANPP on the development and formation of investigated male generative system of vines and fruit trees. Thus, on the base of obtained results we can't confirm its deleterious effect on the environment.

• Applied Microscopy
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• v.32 no.4
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• pp.345-360
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• 2002

Pollen morphology of four genera, 22 species of Korean Crassulaceae has been examined with light and scanning electron microscopy. The presence/absence of the aperture margo and granules was useful characters to clarify the generic boundaries, and the shapes of the streate were significant to delineate taxonomic relationships within the genus Sedum. The pollen grains of Korean Crassulaceae were small and medium in size. Hylotelephium viviparum has the smallest pollen grains, and H. viridescens the largest. The pollen grains are tricolporate, circular in polar view and were prolate to prolate-spheroidal in equatorial shape. Korean Crassulaceae species have striate grains. The close pollen morphological relationships among Hylotelephium, Meterostachys and Orostachys species were concordant to the results based on the previous molecular and morphological studies. The results of the clustering analysis using pollen characters support the segregation of Sedum sensu lato which has been proposed by the previous authors. Within the genus Sedum, S. kamtschatium is closely related to S. middendorffianum with granules on the surface of the pollen grains. Sedum polytrichoides, a member of the Sedum, was clustered to the species of the Meterostachys, Hylotelephium and Orostachys, and needs to reexamine the taxonomic status. O. malacophyllus collected at Chungdong-Jin was significantly differ from the same species from Gampo in pollen and morphological characters.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.129-129
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• 2017

The first key point to the successful pollination and fertilization in plants is the pollen pistil interaction, referring to the cellular and molecular levels, which mainly play active roles in limiting gene flow among maize populations and between maize and teosinte. This study was carried out to identify proteins and investigate the mechanism of gametophytic factors using protein analysis. W22 (ga1); which didn't carry a gametophytic factor and W22 (Ga1), a near iso-genic line were used for the proteome investigation. SDS-PAGE was executed to investigate proteins in the pollen and pistil of W22 (ga1) and W22 (Ga1). A total of 44 differentially expressed proteins were identified in the pollen and pistil on SDS-PAGE using LTQ-FTICR MS. Among the 44 proteins, a total of 24 proteins were identified in the pollen of W22 (ga1) and W22 (Ga1) whereas 20 differentially expressed proteins were identified from the pistil of W22 (Ga) and W22 (Ga1). However, in pollen, 2 proteins were identified only in the W22 (ga1) and 12 proteins only in the W22 (Ga1) whereas 10 proteins were confirmed from the both of W22 (ga1) and W22 (Ga1). In contrary, 10 proteins were appeared only in the pistil of W22 (ga1) and 7 proteins from W22 (Ga1) while 3 proteins confirmed in the both of W22 (ga1) and W22 (Ga1). Moreover, the identified proteins were generally involved in hydrolase activity, nucleic acid binding and nucleotide binding. These results help to reveal the mechanism of gametophytic factors and provide a valuable clue for the pollen and pistil research in maize. In addition, it might provide a comprehensive insight on the proteins that were involved in the regulation of pollen-pistil interaction.

• Molecules and Cells
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• v.38 no.6
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• pp.506-517
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• 2015

Arabidopsis Shaggy-like protein kinases (ASKs) are Arabidopsis thaliana homologs of glycogen synthase kinase 3/SHAGGY-like kinases (GSK3/SGG), which are comprised of 10 genes with diverse functions. To dissect the function of $ASK{\beta}$ (AtSK32), $ASK{\beta}$ antisense transgenic plants were generated, revealing the effects of $ASK{\beta}$ down-regulation in Arabidopsis. Suppression of $ASK{\beta}$ expression specifically interfered with pollen development and fertility without altering the plants' vegetative phenotypes, which differed from the phenotypes reported for Arabidopsis plants defective in other ASK members. The strength of these phenotypes showed an inverse correlation with the expression levels of $ASK{\beta}$ and its co-expressed genes. In the aborted pollen of $ASK{\beta}$ antisense plants, loss of nuclei and shrunken cytoplasm began to appear at the bicellular stage of microgametogenesis. The in silico analysis of promoter and the expression characteristics implicate $ASK{\beta}$ is associated with the expression of genes known to be involved in sperm cell differentiation. We speculate that $ASK{\beta}$ indirectly affects the transcription of its co-expressed genes through the phosphorylation of its target proteins during late pollen development.

• The Korean Journal of Quaternary Research
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• v.24 no.1
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• pp.25-33
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• 2010

Holocene vegetation and climate changes were assumed on the basis of pollen records from Wonpyeong Trench II-3 of the Osong archaeological site, Cheongju, Chungbuk Province, Korea. An organic matter beared in coarse sediments appeared to be low throughout the succession. Although an occurrence of pollen grains is not high, some dominant and principal taxa may indicate vegetation changes response to climate changes in central inland area of the Korean Peninsula. The age determination can be estimated with indirect way by comparing with previous age-controlled pollen studies. It is assumed that the former last glacial conifer forests had been changed into open mixed conifers and deciduous broadleaved forest during the early Holocene period. Warmer and more humid climate conditions, during the mid-Holocene, might have allowed the hardwoods including deciduous- and evergreen-broadleaved trees, and warm-preferring pine tree to flourish. Subsequently, the former forests were replaced by mixed of conifer and deciduous broadleaved forest owing to deterioration of climate conditions during the late Holocene. Human activity is also detected by agricultural indicators, such as buckwheat and large pollen grains comparable to corn, in upper most pollen profile. During this time, the forests in studied area were primarily affected by human disturbance rather than natural environment.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.43 no.4
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• pp.250-253
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• 1998

Somaclonal variation was observed in the field on doubled haploid plants derived from single pollen of a rice cultivar "Hwaseongbyeo". The variations of seven quantitative traits including plant height and one qualitative trait (pubescence) in 436 lines ($R_2$ generation) were analyzed. The number of lines which fell beyond the boundaries of the 95% confidence intervals of the check variety, Hwaseongbyeo was checked for each quantitative trait, and of those fertility showed the highest variation frequency (85.6%), followed by plant height (77.5%), flag leaf length (66.5%), grains per panicle (42.2%), days to heading (34.5%), panicle length (30.7%) and panicles per hill (22.7%). And the variations of quantitative traits except days to flowering appeared to move in the negative direction compared to "Hwaseongbyeo". Variability within lines was also observed for quantitative and qualitative traits. Twenty-nine $R_2$ lines (7%) segregated for pubescence and 130 $R_2$ lines (30%) showed variation with regard to fertility. This suggests that mutations usually occur before diploidization. Twenty-nine $R_2$ lines representing a wide spectrum of variation were chosen for RAPD analysis. The number of lines showing DNA polymorphism compared to Hwaseongbyeo ranged 0 from to 10 according to the primer used and this seems to indicate that specific loci have highly mutable genomic site.utable genomic site.

• The Korean Journal of Ecology
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• v.28 no.3
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• pp.121-127
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• 2005

To reveal vegetation history and environmental changes in Mt. Jiri, sediment cores were collected from Wangdeungjae moor of Mt. Jiri. Overall dry matter accumulation rates and sedimentation rates by $^{14}C-dating$ were 0.027 $kg{\cdot}m^{-2}{\cdot}yr^{-1}$ and 0.184 mm/yr since 1250 ($760{\pm}40$ yrs BP, 14 cm in depth). There are three pollen zones; the first zone is below 14 cm depth where Quercus dominated, the second zone is from 14 cm to 6 cm depth where Gramineae increased and Quercus and Salix dominated and the third zone is from 6 cm depth to the top where Pinus and Quercus dominated. Total pollen concentration gradually increased from bottom to the top of sediment core, which implies wet, anaerobic and cool condition during covered period by the core. Calcium and magnesium contents had increased since 14 cm depth, with peaks at 13 and 20 cm depths. This indicates that groundwater had recently become relatively more important than surface water as water source of Wangdeungjae moor Exotic plant or Chenopodiacea pollen was less than 1%. There was little variation in total N and P contents along the length of the core. These results support that Wangdeungjae moor has been little affected by anthropogenic activities. Also, nutrients and heavy metal contents indicate the baseline condition of Wangdeungjae moor.

• Journal of Korea Foresty Energy
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• v.22 no.1
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• pp.30-36
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• 2003

2kg of pollen extracted with EtOH(98%), concentrated, and fractionated with a series of hexane, CH$_2$Cl$_2$ EtOAc and $H_2O$ on a separately funnel. Each fraction was freeze dried to give dark-brown powder and EtOAc soluble portion of the powder was chromatographed on a Sephadex LH-20 column using a series of aqueous methanol as eluents. Spectrometric analysis such as NMR and FAB-MS including TLC were performed to characterize the structures of isolated compounds. Pollen contained a small amount of flavonol derivatives such as quercetin-3-O-$\beta$-D-glucopyranoside and kaempferol-3-O-$\beta$-D-rutinoside in addition to a small amount of flavanonol compound such as aromadendrin-5-methyl ether and acid compound such as.