• Journal of Species Research
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• 제4권2호
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• pp.159-168
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• 2015

Although airborne pollen is invisible to the eye, it has been known as a major source to respiratory allergic reactions. For this reason, airborne pollen is monitoring in many countries to predict pollen concentration based on locality and season. However, the morphological characteristics of airborne pollen and their potential tendency as an allergen are still obscure. In the present study, we selected 52 airborne pollen samples based on previously reported data and investigated their detail pollen characteristics using LM and SEM. Major airborne pollen in Korea has sorted in 19 families (most angiosperms except four gymnosperm families), and all pollen grains are small to medium in size ($P=17.34-49.86{\mu}m$) apart from the bisaccate pollen grains of Pinaceae ($P=46.49-106.20{\mu}m$). The aperture number and shape vary from sulcate to polyporate. While the inaperture pollen has found only in gymnosperm (Cupressaceae and Taxaceae), triporate or polyporate is common pollen type in angiosperm. The sexine ornamentations could divide into several types, but the most sculpturing types are inconspicuous like psilate, rugulate and granulate. Reticulate pollen grains as a semitectum have occurred the species of genera Platanus and Fraxinus only. To estimate the possible relationships between pollen features and allergen, the results are discussed in botanical context.

• Journal of Plant Biology
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• 제30권4호
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• pp.267-276
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• 1987

A pollen analytical study was carried out on the accumulated humus samples collected from 27 sites under the different plant communities of Mt. Seolag. These pollen spectra were then compared with the actual vegetation. The aboreal pollen(AP) and non-abnoreal pollen(NAP) were 1 Family 25 Genus and 5 Family 5 Genus, respectively. Among the aboreal pollen identified, the pollen of Pinus, Quercus, Betula and Acer were appeared in abundance and the coniferous pollen of Pinus was occupied at least 20%-65% through all the sites studied. The pollens of Abies, Betula, accer, Quercus, Carpinus, Corylus, Fraxinus, Styrax, Prunus and Ericaceae were in accordance with the present vegetation but the other pollens were inconsistent with the actual vegetaton. It suggests that it was caused by the difference between Wehseolag and Namseolag of subalpine zone in Mt. Seolag was 200 m by pollen spectra. Quercus was distributed at the lower part of Namseolag and the subalpine boreal elements were increased with increasing altitudes. The pollen spectra of each site was put together into 5 groups of pollen assemblage by cluster analysis and the community coefficient (CC) was over 60% between all sites studied.

• The Plant Pathology Journal
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• 제38권3호
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• pp.220-228
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• 2022

Pollen is a vector for viral transmission. Pollenmediated viruses cause serious economic losses in the fruit industry. Despite the commercial importance of pollen-associated viruses, the diversity of such viruses is yet to be fully explored. In this study, we performed metatranscriptomic analyses using RNA sequencing to investigate the viral diversity in imported apple and kiwifruit pollen. We identified 665 virus-associated contigs, which corresponded to four different virus species. We identified one virus, the apple stem grooving virus, from pear pollen and three viruses, including citrus leaf blotch virus, cucumber mosaic virus, and lychnis mottle virus in kiwifruit pollen. The assembled viral genome sequences were analyzed to determine phylogenetic relationships. These findings will expand our knowledge of the virosphere in fruit pollen and lead to appropriate management of international pollen trade. However, the pathogenic mechanisms of pollen-associated viruses in fruit trees should be further investigated.

• 식물분류학회지
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• 제53권1호
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• pp.9-13
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• 2023

Menyanthes trifoliata L., a heterostyly plant, is an endangered species in Korea. Floral morphology has been published for M. trifoliata, but few works have concentrated on pollen dimorphism differences accompanied by floral dimorphism. Here, we conducted a comparative morphological study of two morphs to investigate a dimorphism system with Korean populations. Pollen morphological characteristics were observed with a light microscope and a scanning electron microscope. For the pin type, pollen size is 36.01 ± 2.01 ㎛, whereas for the thrum type it is 41.28 ± 2.58 ㎛ in terms of the equatorial diameter. The two morphs have a small apocolpium field at 5.62 ± 0.30 for the pin type and 6.24 ± 0.70 for the thrum type. The configuration of the aperture was tricolpate with a striate ridge in the two morphs. However, they have different pollen shapes and sizes, stigma shapes, and apocolpium sizes. M. trifoliata only has different pollen sizes and shapes between European populations and Korean populations. Nevertheless, Korean populations also show pollen dimorphism correlated with their floral dimorphism.

• Plant Resources
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• 제8권2호
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• pp.87-90
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• 2005

In tobacco, in vitro pollination has been successfully applied to overcome interspecific incompatibility. The use of this technique will make it possible to introduce DNA into pollen tubes just before fertilization. In this study, we showed improvement of the efficiency of in vitro self-pollination and introduction of foreign genes into pollen tubes by the method of polycation. A plasmid harbouring the GUS gene was introduced into pollen grains and pollen tubes, which had incubated on pollen germination medium(PGM), by polyornithine method. Transient expression of the GUS in pollen grains and pollen tubes that were treated with 0, 2, 5 and $10{\mu}g/m\ell$ DNA was observed. In results, combination of the techniques of polyornithine and in vitro pollination was efficient new technique for genetic transformation through fertilization processes.

• Journal of Plant Biotechnology
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• 제4권4호
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• pp.151-154
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• 2002

Conditions for lily pollen growth in vitro and transformation were optimized. Active pollen tube development was achieved effectively in a medium containing 7% sucrose with pH adjusted to 5.7 at the temperature of 27$^{\circ}C$ for about 16-24 hours. Pollen growth was little impaired by the presence of kanamycin at concentration up to 100 mg/L. Pollen rains near the beginning of germination stage were more reliable for Agrobacterium-mediated GUS DNA transformation via vacuum infiltration lasted for 20-40 minutes. GUS DNA integration and its expression in fully developed pollen tubes could be confirmed by Southern blot hybridization, RT-PCR and histochemical staining.

• Journal of Plant Biology
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• 제9권1_2호
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• pp.17-21
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• 1966

The mutual relationships of boron, Ca and sucrose were studied in relation to in vitro pollen growth and pollen bursting, by using conventient pollen from Crinum asiaticum for experiment. Crinum pollen are paticularly sensitive to Ca. Addition of very small amount of boron to cultural media was apparently synergistic to the action of sucrose and Ca in pollen germination and tube elongation. This action was extended to a higher level of boron concentraton. Combined application of boron, Ca and sucrose always gave the better results in pollen growth and protection against pollen bursting much more than when used singly. This indicated that there is a direct relationship between better growth of pollen and increased rigidity of pollen cell wall. A higher level of Ca concentration tended to increase bursting rate of pollen grains and decrease that of pollen tubes, while boron always depressed the rate of bursting. This was considered due to increased failure in pollen germination at high level of Ca that favors pollen tube elongation. The fact that Ca show an antagonistic effect on the suppressive action of high level of boron in pollen growth and shows different effect in response to pollen bursting from boron, suggested mode of Ca and boron action in the presence of sucrose is quiate different, although to increase in rigidity of pollen cell wall by them is in common nature. It was postulated therefore that Ca acts on pectins of pollen cell wall largely as "non-metabolic" and boron as "metabolic" promoter is pollen growth and protecting pollen bursting, since boron and Ca have common nature in strengthening the pollen cell wall but act differently.but act differently.

• 식물조직배양학회지
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• 제22권1호
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• pp.13-17
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• 1995

작약의 화분배양에 의한 반수체 식물을 유기시킬 목적으로 화분배양을 실시하여 배양중 소포자의 변화를 관찰하고 캘러스 및 배발생에 미치는 저온전처리(5$^{\circ}C$/10 days)의 효과와 배양화분의 생존율을 조사하였다. 작약의 화분배양시 캘러스 및 배발생에는 저온전처리의 효과가 다소 인정되었으나 약배양의 효과에는 미치지 못하였다. 화분를 첨가한 고체배지에 이식했을 때 구형배가 형성되었다. 화분의 생존율에는 저온전처리 및 배지조성간 의 영향이 미치지 않았으며 배양기간이 지남에 따라 화분의 생존율도 저하되었다.

• Journal of Plant Biology
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• 제39권4호
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• pp.273-278
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• 1996

Arabidopsis mutants deficient in flavonoid and sinapate ester (tt4 and fah1-7, respectively) were evaluated in vivo and in vitro to study the possible role of flavonoid compounds in pollen tube growth. In vivo, we investigated pollen tube growth in the pistils of the mutants and wild type(Ler). The growth of pollen tubes was significantly different among the three genotypes. In the fal1-7 pistils, the tubes grew to a greater length relative to those of the wild type or tt4. To examine in vitro pollen tube growth, a solid medium was devised for pollen germination and subsequent growth. In vitro, the identical result was obtained; fahl-7 pollens developed the longest tubes and elongated most rapidly. Therefore, the growth response of pollen tubes to phenolic compounds was examined by adding quercetin or sinapate ester in various concentrations to the media. Quercetin enhanced both germination rate and tube growth in the pollens of the mutants and the wild type, especially in tt4. In contrast, sinapate ester inhibits pollen germination and pollen tube growth in three genotypes. These results suggest that flavonoids and related phenolic compounds have physiological role in the plant reproductive system.

• 식물조직배양학회지
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• 제27권5호
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• pp.411-413
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• 2000

The ability of microspores or young pollen grains (male gametophytes) to undergo developmetal switch to embryogenic (sporophytic) pathway exemplifies the concept of totipotency as applied to haploid posmeiotic cells. As a first step pollen is devoid of positional information provided in situ by the intact anther - by isolation and cultivation in vitro in artificial media. This is inevitably accompanied by some degree of stress response in microspore/pollen. It has been shown in both monocots and dicots that intentional stress treatment (mostly starvation or heat shock) greatly stimulates embryo induction rate. Using transgenic sHSP antisense Nicotiana tabacum we show that expression of small heat shock proteins is an integral part of successful embryo and later haploid plant production from pollen grains. Our recently published data show that sHSP chaperone function is optimal in the absence of ATP.