• 제목/요약/키워드: polarizing light microscope

검색결과 26건 처리시간 0.032초

아르곤 레이저 광감각법의 법랑질 우식증 조기탐지 효과에 관한 연구 (A STUDY ON THE EARLY DETECTION OF ENAMEL CARIES BY THE LUMINESCENCE EXCITED BY ARGON LASER)

  • 이난영;이창섭;이상호
    • 대한소아치과학회지
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    • 제24권1호
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    • pp.313-324
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    • 1997
  • The aim of the present study was to describe an safe and convenient method for the early detection of enamel caries using laser fluorescence. Fluorescence from natually carious lesion of human teeth illuminated by an argon laser(488nm) was observed and photographed using barrier filter. Intact enamel was found to fluorescence with a yellowish light. Whereas, incipient caries lesions in the enamel were dearly visible as dark areas in contrast to the fluorescence surroundings. For evaluation of accuracy of this method, lesion depth measured by the laser fluorescence in light microscope was compared with that polarizing microscope. The results from the present study can be summarized as follows : 1. Enamel caries of smooth surface was observed as pale white spot and undefined outline in ordinary light. Whereas, lesion was clearly visible as dark spot in laser fluorescence. 2. There was no difference between ordinary light view and laser fluorescence in occlusal surface and interproximal surface. 3. There was no significant difference between the lesion depth observed by laser fluorescence with light microscope and polarizing microscope. Apparent correlation exists between two groups.

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초기우식병소에 대한 레이저 fluorescence의 광학적 탐지감도 (OPTICAL SENSITIVITY OF LASER FLUORESCENCE FOR INCIPIENT CARIES DETECTION)

  • 김효석;김왕근;이창섭;이상호
    • 대한소아치과학회지
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    • 제26권1호
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    • pp.109-118
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    • 1999
  • The aim of this study was to evaluate the optical density of laser fluorescence for detection of incipient caries. Prepared and polished bovine enamel specimens were demineralized in a STPP solution for varying periods of time between 3 hrs. and 60 hrs. with an area of sound enamel retained on each specimen. The randomized specimens were analyzed for optical density of enamel demineralization using laser fluorescence. The specimens were sectioned and examined lesion depth by polarizing light microscope. Results were analyzed statistically with SAS program. The results from this study can be summarized as follows: 1. Optical density measured by laser fluorescence and lesion depth measured by polarizing light microscope was increased as demineralization time was increased(p<0.001). 2. Between optical density measured by laser fluorescence and lesion depth measured by polarizing light microscope was correlated highly(${\gamma}{\geq}0.74956$, p<0.001). 3. Regressive equation was obtained in this study as follows. Y=[X-0.260851]/0.000271(R-square:0.5618, p<0.001) (X:DENSITY, Y:DEPTH) In summary, optical density measured by laser fluorescence would be within the range of possibility to quantitatively presume demineralization amount of incipient caries lesion

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광중합형 및 자가중합형 교정용 전색제의 치아우식예방 및 진행억제효과에 관한 실험적 연구 (The effects of fluoride releasing orthodontic sealants on the prevention and the progressive inhibition of enamel demiheralization in vitro)

  • 채승원;;윤영주;김광원
    • 대한치과교정학회지
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    • 제27권6호
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    • pp.979-995
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    • 1997
  • 고정성 교정장치 주변에 발생되는 백색반점은 임상적으로 쉽게 눈에 띄는 문제점을 가지고 있으므로 잔치제거 후 심니적으로 만족스럽지 못한 결과를 야기하는데, 이는 곧 교정치료의 실패를 의미한다. 이미 많은 연구를 통해 고정성 교정장치가 구강내 세균환경을 변화시켜 세균집단의 증식을 용이하게 함으로써 고정성 장치 부착 후, 법랑질 탈회의 발생빈도가 증가된다는 사실이 보고 되어 왔으며 장기간이 소요되는 교정치료의 특성으로 인해 발생되는 브라켓 또는 교정용 밴드 주변의 법랑질 탈회나 치아우식증을 예방 또는 억제시키기 위한 연구가 함께 진행되어 왔다. 이는 환자에 대한 구강위생 교육이나 치아관리 노력에도 불구하고 발생될 수 있는데 이를 예방하기 위한 방법의 하나로 불소가 유리되는 교정용 전색제를 사용할 수 있다. 본 연구는 불소가 유리되는 광중합형 및 자가중합형 교정용 전색제의 치아우식 예방 및 진행억제효과를 규명하기 위해 다음과 같이 각각 7개의 편광현미경군(A군-G군)과 주사전자현미경군(A'군-G'군)으로 분류하였고 [1. A & A'군 STPP 인공우식용액에 담그지 않은 정상치아군, 2. B & B'군 : STPP 인공우식용액에 담그고 $37^{\circ}C$의 항온용기 에 72시간 보관한 비처치군,3. C & C'군 : STPP 인공우식용액에 담그고 $37^{\circ}C$ 의 항온용기에 72시간 보관한 자가중합형 교정용 전색제군, 4. D &D'군 : STPP 인공우식용액에 담그고 $37^{\circ}C$의 항온용기에 72시간 보관한 광중합형 교정용 전색제군, 5. E & E'군 표면에 아무처리 없이 STPP 인공우식용액에 담그고 $37^{\circ}C$의 항온용기에 72시간 보관한 후, 이를 다시 아무런 처치없이 STPP 인공우식용액에 담그고 $37^{\circ}C$의 항온용기에 72시간 보관한 비처치군, 6. F & F'군 : 표면에 아무런 처치없이 STPP 인공우식용액에 담그고 $37^{\circ}C$의 항온용기에 72시간 보관한 후, 자가중합형 교정용 전색제를 도포하고 이를 다시 STPP 인공우식용액에 담그고 $37^{\circ}C$의 항온용기에 72시간 보관한 자가중합형 교정용 전색제, 7. G & G'군 :표면에 아무런 처치없이 STPP 인공우식용액에 담그고 $37^{\circ}C$ 의 항온용기에 72시간 보관한 후, 광중합형 교정용 전색제를 도포하고 이를 다시 STPP 인공우식용액에 담그고 $37^{\circ}C$ 의 항온용기에 72시간 보관한 광중합형 교정용 전색제군], 이들을 편광현미경과 주사전자현미경을 이용해 조직의 변성 여부를 검색하여 다음과 같은 결과를 얻었다. 1. 편광현미경적 연구에서 인공우식병 소의 깊이는 $A군(5.08{\mu}m),\;B군(47.82{\mu}m,\;C군(8.42{\mu}m),\;D군(7.20{\mu}m),\;E군(85.41{\mu}m),\;F군(60.38{\mu}m),\;G군(60.13{\mu}m)$,이었다. 2. 인공우식 병소의 깊이에 있어서, B군은 A군,C군,D군과 비교하여 통계학적으로 유의성 있는 차이를 보였고(p<0.05), E군은 F군,G군과 비교하여 통계학적으로 유의성 있는 차이를 보였다(p<0.05). 3. 광중합형 및 자가중합형 교정용 전색제는 법랑질탈회 예방효과가 있었다. 4. 광중합형 및 자가중합형 교정용 전색제는 법랑질탈회 진행억제효과가 있었다. 5. 광중합형 및 자가중합형 교정용 전색제 표본에 대한 주사전자현미경적 연구에서 인공우식용액의 시간 경과에 따른 영향은 없었다. 6. 광중합형 교정용 전색제 표본과 자가중합형 교정용 전색제 표본 사이의 차이는 편광현미경과 주사전자현미경으로 구별할 수 없었다.

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시료 채취 조건 및 검사방법에 따른 지하수내 섬유상 물질 검출 양상에 관한 연구 (Effect of Sampling and Analytical Methods on the Fibrous Materials from the Ground Water)

  • 김지용;김정란;정해관;임현술;백남원
    • 한국산업보건학회지
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    • 제7권2호
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    • pp.209-222
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    • 1997
  • Authors surveyed the ground water near the waste disposed from a fiberglass production factory to confirm the presence of glassfiber in the water and to determine the effect of sampling conditions and storage on the recovery of fibrous materials in the ground water. Sample was collected at every 4 hours for 48 hours consecutively. After finishing the 48 hours sample, water sampling was done from each tap after repeated turning on and off the water for 30 seconds at each time. Sample was collected in the two 1.5 liter polyethylene bottle after vigorously shaking the bottle with the same water several times with the flowing tap water. At each paired sample, one bottle was stored stand still at room temperature, and the other sample was filtered immediately after sampling. Water was filtered on the Mixed Cellulose Ester filter with negative pressure. Each sample was divided into upper and lower layer. The other bottle was stored at room temperature standstill for 7 days and filtered in the same fashion as the other pair of sample did. Each MCE filter was divided into 4 pieces and one piece was treated with acetone to make it transparent. Each prepared sample was observed by two researchers under the light and polarizing microscopy, scanning electron microscopy and energy dispersive X-ra microanalysis. Fibers were classified by the morphology and polarizing pattern under the polarizing microscope, and count was done. 1. There was a significant fluctuation in number of the fibers, but there was no specific demonstrable pattern. 2. Non-polarizing fibers frequently disappeared after 7 days's storage. But cluster of fibers were found at the wall of the same container by scratching technique. 3. Polarizing fibers were usually found in between the filter and the manicure pasted area. Possible explanations for this phenomenon will be that either these fibers are very light or have electronic polarity. Hence, these fibers are not able to be attached on the surface of slide glass. 4. Under the scanning electron microscopic examination, the fibers which are not refractive under the light microscopy were identified as glassfiber. Other fibers which is refractive under the polarizing microscopy were identified as magnesium silicate fibers. It is strongly suggested that development of standardized method of sample collection and measurement of fibrous material in the water is needed.

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Synthesis and Properties of Liquid Crystalline Y-shaped Molecules Containing 1,3,4-Oxadiazole

  • Kwon, Eun-Kyoung;Choi, E-Joon
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2007년도 7th International Meeting on Information Display 제7권1호
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    • pp.542-544
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    • 2007
  • New liquid crystalline Y-shaped molecules containing 1,3,4-oxadizoles have been synthesized with variation of terminal groups (R = H, $OCH_3$ or $OC_8H_{17}$). The structures of obtained compounds were identified by FT/IR and NMR spectrometry, and their thermal and liquid crystalline properties were investigated by DSC and polarizing optical microscope.

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Photoresponsive Behavior of Liquid-Crystalline Networks

  • Yu, Yanlei;Ikeda, Tomiki;Nakano, Makoto
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2002년도 International Meeting on Information Display
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    • pp.34-37
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    • 2002
  • Freestanding azobenzene-containing liquidcrystalline network (LCN) films. with macroscopic uniaxial molecular alignment were prepared by insitu photopolymerization. By polarizing microscopy, fiber-like structures aligned in one direction were observed. Furthermore, with a confocal laser scanning microscope (CLSM), it was confirmed that the fiber-like structures were formed even in the bulk of the LCNs. Upon UV light irradiation to cause trans-cis photoisomerization of the azobenzene molecules, the LCNfilms underwent a significant and anisotropic bending toward the irradiation direction of UV light. When the bent LCNfilms were exposed to Vis light, unbending of the LCN films immediately took place and the initial flat LCN films were restored. This bending and unbending behavior of the LCN films could be repeated just by changing the wavelength of the irradiation light. It was suggested that the bending was induced by an absorption gradient which produced a volume difference between the front surface area and the bulk of the network films.

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화순지역 천운산층 중의 점토암의 조직과 그 성인적 의미 (Textures of Claystones and Their Genetic Significances in the Cheonunsan Formation of the Hwasoon Area)

  • 김수진;추창호;이동진
    • 한국광물학회지
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    • 제11권1호
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    • pp.13-19
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    • 1998
  • Textures of claystones of the Cheonunsan Formation in the Hwasoon area have been studied using optical microscope and electron microprobe. Microscopic images were observed under the optical microscope using the transmitted polarizing light from thin sections and under the electron microprobe using the back-scattered electron beam from the polished sections. Identification of minerals were made using X-ray diffraction analysis and chemical analysis by electon microprobe. Textural analyses show that the original sedimentary claystones rich in aluminium were subjected to metamorphism by which they changed to the metamorphosed claystone consisting mainly of chloritoid, quartz, andalusite and illite. Later intensive hydrothermal kaolinization of this metamorhosed claystones resulted in the formation of high-aluminous claystones rich in kaolinite exhibiting various complicated replacement textures.

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목재세포벽(木材細胞壁)의 미세구조(微細構造)에 관한 연구(硏究) - Compression wood의 나선비후(螺旋肥厚)의 구조(構造) - (Ultrastructure of Wood Cell Wall Tracheids - The Structure of Spiral Thickenings in Compression Wood -)

  • 이원용
    • Journal of the Korean Wood Science and Technology
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    • 제9권1호
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    • pp.1-12
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    • 1981
  • The structure of spiral thickenings, particulary the appearance, arrangement and orientation of thickenings in compression wood of Torreya nucifera, were studied in detail by light and polarizing microscope, scanning and transmission electron microscope. The results obtained are as follows: (1) Using the inclined sections at an angle of 45 degrees to the fiber axis, it seems that we can not only observe the more accurate transverse view of the thickenings but also investigate the formation of their thickenings. (2) Generally 2-4 pieces of thickenings are projected to the cell lumen as nipple-like appearance in transverse section and are as frequent, well developed, forming pair and have the rope-like appearance in radial surface. (3) The secondary wall of early wood is composed of 3 layers (S1, S2, S3) and orientation of thickening appears S helix but that of late wood is of 2 layers (S1, S2) and that orientation shows Z helix. Above two regions are demaracted at several tracheid cells from the growth ring boundary. (4) Orientation of thickening seems to be a element showing the characteristics of compression wood in Torreya nucifera. (5) It believes that the thickenings of compression wood are integral part of the S3 in early wood tracheids and of the S2 in late wood and have the same orientations as the inner-most microfibrils in these layers. (6) Thickening and cavities seem to be not formed together in a secondary cell wall of same tracheids.

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Orientational deformation of ferroelectric liquid crystal molecules by bending performance of plastic substrate

  • Son, Ock-Soo;Lee, Ji-Hoon;Jang, Chi-Woong;Lim, Tong-Kun
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.I
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    • pp.338-341
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    • 2005
  • We have examined the aspects of the orientational ordering deformation of ferroelectric liquid crystal during bending performance of plastic substrate by analyzing the polarizing optical microscope texture and the birefringence of the cell. Striped texture becomes more prominent as the radius of curvature of substrate gets smaller. The optic axis of the adjacent stripes domain was not same and the relative angle between them becomes larger as the radius of curvature gets smaller. Especially, the optic axis rotation angle of one domain was lager than the other and the liquid crystal molecules in each domain became more coherent. In addition, the birefringence data with obliquely incident light shows the polar direction shift of liquid crystal molecule by bending performance.

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Polarization Phase-shifting Technique for the Determination of a Transparent Thin Film's Thickness Using a Modified Sagnac Interferometer

  • Kaewon, Rapeepan;Pawong, Chutchai;Chitaree, Ratchapak;Bhatranand, Apichai
    • Current Optics and Photonics
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    • 제2권5호
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    • pp.474-481
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    • 2018
  • We propose a polarization phase-shifting technique to investigate the thickness of $Ta_2O_5$ thin films deposited on BK7 substrates, using a modified Sagnac interferometer. Incident light is split by a polarizing beam splitter into two orthogonal linearly polarized beams traveling in opposite directions, and a quarter-wave plate is inserted into the common path to create an unbalanced phase condition. The linearly polarized light beams are transformed into two circularly polarized beams by transmission through a quarter-wave plate placed at the output of the interferometer. The proposed setup, therefore, yields rotating polarized light that can be used to extract a relative phase via the self-reference system. A thin-film sample inserted into the cyclic path modifies the output signal, in terms of the phase retardation. This technique utilizes three phase-shifted intensities to evaluate the phase retardation via simple signal processing, without manual adjustment of the output polarizer, which subsequently allows the thin film's thickness to be determined. Experimental results show that the thicknesses obtained from the proposed setup are in good agreement with those acquired by a field-emission scanning electron microscope and a spectroscopic ellipsometer. Thus, the proposed interferometric arrangement can be utilized reliably for non-contact thickness measurements of transparent thin films and characterization of optical devices.