• Title/Summary/Keyword: polarized microscopy

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The Characteristics of Dispersed Asbestos Fibers Produced From Building Materials (건축재료에서 발생되는 석면입자의 특성 연구)

  • 유성환
    • Journal of Korean Society for Atmospheric Environment
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    • v.9 no.3
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    • pp.191-199
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    • 1993
  • This paper describes the results of a systematic study to determine the characteristics of particle generated from various types of asbestos containing material(ACM) and manmade fiber material(MMFM) during operations of cutting and grinding in laboratory and workplace. Tests were conducted with a specially designed glove box which allowed complete sampling of the generated asbestos fibers. Specificially, air measurements were made during ACM and MMFM installation in building. All personal air samples collected were identified by polarized light microscopy(PLM), X-ray diffraction(XRD) and scanning electron microscope with energy dispersive X-ray analysis(SEM/EDXA). Also, the samples were counted by phase contrast microscope(PCM) in order to compare the results with the permissible exposure standard for workplace. Results indicate that the characterisitcs of fibers found in the roofing sheet, the ceiling and the wall insulation boards were identical to those of asbestos, while the characteristics of fibers found in the ceiling insulation board, the floor tile and the sprayed on insulation products in parking area were identical to those of asbestos, while the characteristics of fibers found in the ceiling insulation board, the floor tile and the sprayed on insulation products in parking area were identical to those of rock wool. The concentrations of airborne fibers from various building materials cut by a grinder for 5 minutes were in the ranges of 0.09 $\sim$ 1.71 fibers/cc(f/cc). The highest concentration(1.71f/cc) was found during grinding the wall insulation board which also contains rock wool. The airborne fiber concentrations generated by installing at workplace were ranged from 0.0009 to 0.029 f/cc. All asbestos fibers from the ceiling insulation board at workplace were less than 20$\mu$m in length and more than 20% of them had the average aspect ratio greater than 20. Therefore, for the purpose of decreasing asbestos and man-made fiber concentrations at the workplace, the ceiling and wall board should use strong binding material to increase the binding force with fiber. Also, the permissible exposure standard for workplace(2.0f/cc) in Korea should be constituted below the maximum avaiable concentration measured at glove box.

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Unusual Alignment of Liquid Crystals on Rubbed Films of Polyimides with Fluorenyl Side Groups

  • Hahm, Suk-Gyu;Lee, Taek-Joon;Chang, Tai-Hyun;Jung, Jin-Chul;Zin, Wang-Cheol;Ree, Moon-Hor
    • Proceedings of the Polymer Society of Korea Conference
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    • 2006.10a
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    • pp.239-239
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    • 2006
  • In the present study, we synthesized CBDA-FDA and its analogue, poly(4,4' -(9,9-fluorenyl)diphenylene pyromellitimide) (PMDA-FDA), and then investigated quantitatively the orientational distributions of the polymer chain segments in the surfaces of their films by using linearly polarized Fourier transform infrared (FTIR) spectroscopy and optical retardation analysis. We also examined the films' surface topographies using high spatial resolution atomic force microscopy (AFM). Further, rubbed films were used to assemble antiparallel and $90^{\circ}-twisted$ nematic (TN) LC cells, and the alignment behaviors, pretilt angles and anchoring energies of the LC molecules in the cells were determined. The films were found to have very interesting surface morphologies and LC alignment behaviors, which have not previously been reported. The observed LC alignments, pretilt angles and anchoring energies are discussed by taking into account the interactions of the LC molecules with the oriented polymer chain segments and the surface morphologies.

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Synthesis of Cholesteric Liquid Crystal and Its Application as a Polarizing Component on the Optical Film (콜레스테릭 액정의 합성과 광학필름용 편광성분으로서의 응용)

  • Kim, Yong-Suk;Lee, Kwang-Yeon;Ahn, Cheol-Heung;Kim, Geon-Joong
    • Applied Chemistry for Engineering
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    • v.19 no.6
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    • pp.661-667
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    • 2008
  • In this study, cholesteric liquid crystal (CLC) was synthesized and applied as a polarizing component on the optical film of back light units (BLU) for LCDs. After mixing CLC with nematic liquid crystal, this mixture was fulfilled in the module consisting of two films and then its amplifying efficiency and polarizing ability for a planarly emitted light were examined to apply as a BLU polarizer film for increasing the brightness of light. The properties of CLC compound were tested by UV/Visible spectroscopy and polarizing optical microscopy (POM). Flexible spacer was made by linear carboxylic acid group of cholesteric derivatives between cholesterol mesogen units for one-axis orientation in each layer. The CLC containing film could be used as a module to increase the ability of polarization and to enhance brightness of BLU and to widen wavelength range by stacking the films.

Effects of Edge Activator on the Droplet Size and Skin Permeation of Hydrated Liquid Crystalline Vesicles (Edge Activator가 수화 액정형 베시클의 입자크기와 피부 침투에 미치는 영향)

  • Lee, Seo Young;Lim, Yoon Mi;Jin, Byung Suk
    • Applied Chemistry for Engineering
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    • v.28 no.6
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    • pp.679-684
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    • 2017
  • Hydrated liquid crystalline vesicles incorporating a edge activator, which confers flexibility to the vesicle membranes, were prepared and niacinamide was encapsulated in them. The formation of liquid crystalline phases and their thermal phase transitions were investigated by polarized optical microscopy and differential scanning calorimetry (DSC), respectively. Droplet sizes of the vesicles were reduced to several tens of nanometers by incorporating edge activators, such as sodium deoxycholate, lysolecithin, or polysorbate 80. The amount of niacinamide permeated into a pig skin increased greatly using the hydrated liquid crystalline vesicles compared to the case where niacinamide was applied in an aqueous solution state. The vesicles incorporating 10% sodium deoxycholate increased the amount of niacinamide permeated nearly four times. These results suggest that edge activators are effective in improving the skin permeability of vesicles.

A Case of Primary Localized Laryngo-tracheobronchial Amyloidosis (원발성 국한성 후두 기관기관지형 유전분증 1예)

  • Lee, Seok Jeong;Lee, Won Yeon;Jung, Soon Hee;Kwon, Woocheol;Lee, Shun Nyung;Lee, Namseok;Kim, Sang-Ha;Shin, Kye Chul;Yong, Seok Joong
    • Tuberculosis and Respiratory Diseases
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    • v.65 no.6
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    • pp.532-536
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    • 2008
  • We report on a case of a patient with laryngo-tracheobronchial amyloidosis who complained of cough, sputum, and hoarseness. A chest X-ray showed consolidation in the right middle lobe. A chest CT scan showed diffuse, irregular narrowing of the tracheobronchial tree and atelectasis of the right middle lobe, with calcification of bronchial wall. Bronchoscopic findings were multinodular submucosal thickening of the right vocal cord, and yellowish multinodular submucosal thickening from the lower trachea through both main bronchi, as well as the lingular division of the left upper lobe, the right middle lobe, and the right lower lobe. The right middle lobe bronchus was nearly obstructed. The diagnosis of amyloidosis was made by multiple ronchoscopic biopsies on the right vocal cord and both bronchi. Pathologic findings were characteristic apple-green birefringence under polarized microscopy with Congo-red stain. The patient had no evidence of systemic amyloidosis. The patient is under conservative symptomatic treatment.

Micromorphological and Chemical Characteristics of Hardwoods Decayed by a Brown-rot Fungus (활엽수 갈색부후재의 미시형태 및 화학적 특성)

  • Cha, Mi Young;Lee, Kwang Ho;Kim, Yoon Soo
    • Journal of the Korean Wood Science and Technology
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    • v.35 no.6
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    • pp.152-158
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    • 2007
  • Micromorphological and chemical characteristics of living Castanea spp. and Tamarix spp. attacked by a brown-rot fungus were examined. Micromorphological features of brown-rotted hardwood species were similar to those observed in softwoods such as losses in birefringence under polarized microscopy, preferential degradation of $S_2$ layer and cracks in the $S_3$ layer. Thinning of the secondary cell wall in fibers was also observed, although it did not seem to be the main symptom. FT-IR analysis showed that bands assigned to cellulose and hemicelluloses decreased or disappeared, whereas the bands assigned to lignin increased. X-ray diffraction analysis exhibited the decrease of relative crystallinity, indicating that degradation of hardwood by a brown-rot fungus was in the advanced stages.

THE INFLUENCE OF pH ON REMINERALIZATION OF ARTIFICIAL DENTAL CARIES (법랑질 인공우식의 재광화에 미치는 pH의 영향에 관한 연구)

  • Kim, Min-Kyung;Kum, Kee-Yeon;Lee, Chan-Young
    • Restorative Dentistry and Endodontics
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    • v.22 no.1
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    • pp.193-208
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    • 1997
  • Much evidence now exists from both in vivo and in vitro studies to support the claim that small caries lesions can 'heal'. But, there are still different views on the mechanism of remineralization. So in order to find the best condition for the remineralization of incipient dental caries in maximum efficient way, the author conducted the experiment which reveals the effect of pH on remineralization. 40 specimens of sound permanent teeth without demineralization or crack, $100{\mu}m$ in thickness, were immersed in lactic acid buffered demineralization solution for 4 days. Dental caries with surface zone and subsurface lesion were artificially produced. All specimens were immersed in lactic acid buffered remineralization solution of pH 4.0, 4.5, 5.0, 5.5 containg fluoride ion for 10 days. The results were obtaind by observing the specimens for every 10 days under polarized microscopy at x25. 1. Remineralization did not occur in entire depth of body of lesion at given degree of saturation and concentration of fluoride ion. 2. The pattern of remineralization has increased according to increase of pH. So it can be concluded that supersaturated solution with fluoride ion can be affected by pH in remineralization of enamel, and pH 5.5 seems to be very effective in remineralization of deep and surface zone of dental caries. However, more complex factors exist in achieving complete remineralization and further continuous researches are needed to clarify the factors.

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Preparation and Characterization of Elastomeric Solid Electrolyte Based on $PEO-EDA-LiClO_4$ Blends ($PEO-EDA-LiClO_4$ 블렌드계 탄성체 전해질의 제조와 특성)

  • Chang, Young-Wook;Joo, Hyun-Seok
    • Elastomers and Composites
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    • v.39 no.1
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    • pp.36-41
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    • 2004
  • Solid polymer electrolytes were prepared by UV irradiation of the blends consisting of poly(ethylene oxide)(PEO), epoxy diacrylate(EDA) and LiClO_4$. Conductivities of the electrolyte films were measured as a function or blend composition, salt concentration and temperature. The electrolyte having the composition of poly(ethylene oxide) (70% by weight)/epoxy diacrylate (30% by weight) with mole ratio of 10 of ethylene $oxide/Li^+$ exhibited a high ionic conductivity of $1.2{\times}10^{-5} S/cm$ at $25^{\circ}C$. This blend is transparent and shows elastomeric properties. Morphological studies by means of differential scanning calorimetry, X-ray diffraction and polarized optical microscopy indicated that the cured epoxy chains in the blends inhibit the crystallization of poly (ethylene oxide) and thereby induce the blend systems to be completely amorphous in certain compositions.

Morphometric analysis on bone formation effect of $\beta-TCP$ and rhBMP-2 in rabbit mandible (토끼의 하악골에서 $\beta-TCP$ 와 rhBMP-2의 골형성 효과에 대한 형태계측학적 연구)

  • Kim, Kyu-Nam;Yang, Jung-Eun;Jang, Jea-Won;Sasikala, Balaraman;Wang, Beng;Kim, Il-Kyu
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.36 no.3
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    • pp.161-171
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    • 2010
  • Introduction: This study was to assess the effectiveness of new bone formation and regeneration by using a rhBMP-2 and $\beta-TCP$ as a carrier in rabbits’mandible. Materials and Methods: The mandibles of 36 rabbits were exposed and cortical bone was penetrated for this study. The experimental subjects were divided into 3 groups each 12 rabbits ; control group, experimental group 1, and experimental group 2. Control group had the defect itself without any treatment, in the experimental group 1, $\beta-TCP$P only was grafted, and in the experimental group 2, rhBMP-2 soaked in $\beta-TCP$ was grafted. The rabbits were sacrificed after 1, 2, 3, 4, 6, and 8weeks, and new bone formation area was examined and measured for bone quantitative and qualitative analysis with light, fluorescent and polarized microscopy. Results: In the experimental group 1, new bone formation from the adjacent host bone was made by osteoconduction, and in the experimental group 2, direct new bone formation by osteoinduction of rhBMP-2 as well as new bone formation by osteoconduction of $\beta-TCP$ were observed. Conclusion: rhBMP-2 of experimental group 2 is very effective in the bone formation in early 2weeks and bone remodelling from 3weeks.

Degradation Characteristics of Wood Cellulose by Ruminal Cellulolytic Anaerobic Bacterium Ruminococcus albus F-40 (혐기성 세균 Ruminococcus albus F-40에 의한 목재 cellulose의 분해특성)

  • Kim, Yoon-Soo;Wi, Seung-Gon;Myung, Kyu-Ho
    • Journal of the Korean Wood Science and Technology
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    • v.25 no.3
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    • pp.83-95
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    • 1997
  • The degradation mode of lignocellulose by anaerobic ruminal cellulolytic bacterium Ruminococcus albus F-40 was investigated. Birchwood holocellulose and filter paper were incubated as the sole carbohydrate sources with using the Hungate techniques. After 2 or 4 days of incubation, samples were employed for chemical and electron microscopic evaluations. The degradation rate of cellulosic substrates and the adhesion rate of bacteria to the substrates increased proportionally with the decrease of relative crystallinity of cellulose, indicating the preferential breakdown of amorphous cellulose, by this bacterium. X-ray diffraction analyses and polarized light microscopy showed, however, that crystalline cellulose was also degraded by R. albus. FT-IR spectra indicated that not only cellulose but hemicellulose was also degraded by this bacterium. Electron microscopic investigations showed the protuberant structures on the surface of R. albus. These structures were much more significant when bacterial cells were grown in the media containing insoluble substrates, such as cellulose, indicating clearly that bacterial protuberant structures were induced by the substrates. Protuberant structures extended from the bacterial cells adhered tightly to the substrates and numerous vesicles covered the surface of cellulosic substrates affected. Cellulosome-like structures were distributed on the cellulose matrix. Electron microscopic works showed that diverse surface organells of R. albus were involved in the degradation of cellulosic materials. SEM examinations showed the breakdown of cellulose by R. albus was proceeded by severeal routes : short fiber formation, defibrillation and destrafication of cellulose microfibril.

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