• Title/Summary/Keyword: polar column

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Anti-cancer and Anti-inflammatory Effects of Mung Bean and Soybean Extracts (녹두 및 대두추출물의 항암 및 항염증 활성)

  • Imm, Jee-Young;Kim, Seok-Joong
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.755-761
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    • 2010
  • The quinone reductase (QR) inducing activities of mung bean and soybean solvent extracts were compared using murine hepatoma cells (Hepa 1c1c7). The mung bean extracts (ethylacetate and ethanol) showed higher chemoprevention index values (7.88-8.22) than those of soybean extracts (2.9-5.2) from four different cultivars. The mung bean extracts also had significantly higher inhibitory effects (47-62% at 100 ${\mu}g$/mL) than the soybean extracts (15-42% at 100 ${\mu}g$/mL) against the production of nitric oxide and prostaglandin E2 in lipopolysccharide stimulated macrophage RAW264.7 cells without cytotoxicity. Among seven recovered fractions of mung bean ethanol extract obtained by C 18 silica flash column chromatography, the most non-polar fraction exhibited the highest chemoprevention index of 10.4.

Automatic Inspection of Reactor Vessel Welds using an Underwater Mobile Robot guided by a Laser Pointer

  • Kim, Jae-Hee;Lee, Jae-Cheol
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.1116-1120
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    • 2004
  • In the nuclear power plant, there are several cylindrical vessels such as reactor vessel, pressuriser and so on. The vessels are usually constructed by welding large rolled plates, forged sections or nozzle pipes together. In order to assure the integrity of the vessel, these welds should be periodically inspected using sensors such as ultrasonic transducer or visual cameras. This inspection is usually conducted under water to minimize exposure to the radioactively contaminated vessel walls. The inspections have been performed by using a conventional inspection machine with a big structural sturdy column, however, it is so huge and heavy that maintenance and handling of the machine are extremely difficult. It requires much effort to transport the system to the site and also requires continuous use of the utility's polar crane to move the manipulator into the building and then onto the vessel. Setup beside the vessel requires a large volume of work preparation area and several shifts to complete. In order to resolve these problems, we have developed an underwater mobile robot guided by the laser pointer, and performed a series of experiments both in the mockup and in the real reactor vessel. This paper introduces our robotic inspection system and the laser guidance of the mobile robot as well as the results of the functional test.

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Comparison of EM 120 Multibeam Echo Sounding Data with SeaBeam 2000 Data Acquired at KODOS(Korea Deep Ocean Study) Area (한국 심해연구지역에서 획득된 EM 120과 SeaBeam 2000의 자료 비교)

  • Lee, Gun-Chang;Kim, Hyun-Sub;Ko, Young-Tak;Jung, Mee-Sook;Ham, Dong-Jin;Kim, Jong-Uk
    • Ocean and Polar Research
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    • v.28 no.4
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    • pp.467-473
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    • 2006
  • The EM 120, a newly installed multibeam echo sounder on RM Onnuri, has several advantages over the previously installed SeaBeam 2000 in performance and thus data quality. The EM 120 system provides a bottom topographic map with high resolution by (1) increasing the number of beams, (2) increasing the across track swath range, (3) measuring the more accurate sound velocity within the water column, and (4) improving stabilization for pitching, rolling, and yawing of the ship. This study compares EM 120 and SeaBeam 2000 echo sounders in terms of the data quality from the same survey area in the Clarion-Clipperton Zone, NE Pacific. Our result shows that the EM 120 provides more precise topographic data than the SeaBeam 2000. Although overall trends of data, such as topographic direction and relief, are similar for both echo sounders, the water depths measured by the EM 120 are shallower than that of SeaBeam 2000 by 80 to 90 meters.

Ecological Model Experiments of the Spring Bloom at a Dumping Site in the Yellow Sea (생태계모델을 이용한 황해투기해역에서의 춘계 식물플랑크톤 대증식 연구)

  • Song, Kyu-Min;Lee, Sang-Ryong;Lee, Seok;Ahn, Yu-Hwan
    • Ocean and Polar Research
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    • v.29 no.3
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    • pp.217-231
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    • 2007
  • To explore limiting factors of spring bloom caused by waste disposal after dumping activity commenced in the Yellow Sea, we used a 1-dimensional temperature-ecological coupled model. The vertical structure of temperature and vertical diffusivity (Kh) are calculated by the temperature model with sea surface temperature using the 2.5 layers turbulence closure scheme. The ecological model applied results at the temperature model consisted of five state variables (DIN, DIP, phytoplankton, zooplankton, and detritus) forced by photosynthetically available radiation. We simulate year-to-year variations of plankton and nutrients using the coupled model from 1998 to 2000 and compare results of the model with observed data. It turned out that temperature is the growth factor of spring bloom in dumping area. During the winter the weak stratification made sufficient supply of the accumulated nutrients from the sea bed into the upper water column and led to the bloom in the coming spring. Radiation also turned out to be another important factor of spring bloom in the study area. Insufficient radiation of March 1999 showed low chlorophyll-a concentration despite sufficient nutrients in the surface.

Underwater Glider: Its Applicability in the East/Japan Sea (해양 글라이더에 관하여: 한국 근해에서의 적용 가능성)

  • Park, Jong Jin
    • Ocean and Polar Research
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    • v.35 no.2
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    • pp.107-121
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    • 2013
  • The underwater glider is an autonomous vehicle that can glide through the ocean interior by using a pair of wings attached to its body and can move up and down through the water column by changing its buoyancy. As of now, there are three widely-used gliders, namely, the Spray that was co-developed by Scripps Oceanographic Institution and Woods Hole Oceanographic Institution, the Slocum produced by the Webb Research Cooperation, and the Seaglider that was produced by the University of Washington. In this paper, I will introduce these three gliders and discuss the principles and procedures related to glider operation as well as the application and extendability of modern physical and bio-geochemical sensors to gliders. My experiences in developing a glider for measuring ocean turbulence and testing it 7 times during 12 days are shared in this paper. On the basis of my experiences and knowledge, different kinds of aspects that should be considered for successful glider operation are discussed. In addition, a suggestion is made as to what would be the ideal way to operate underwater gliders in the East/Japan Sea. At the end, the current status of active glider operation teams is presented and the efforts to proceed toward future gliders are briefly introduced.

Separation of 20(R&S) Prosapogenin Isomers of Ginsenoside-$Rg_2$ and -$Rg_3$ from Ginseng Saponins by Reversed-Phase High Performance Liquid Chromatography (인삼 사포닌에서 Ginsenoside-$Rg_2$와 -$Rg_3$의 이성질체인 20(R&S) Prosapogenin들의 역상 고속 액체 크로마토그래피에 의한 분리)

  • Jeong, Seung-Il;Kim, Cheon-Suk;Lee, Yong-Gu;Lee, Ho-Sup;Kim, Il-Kwang
    • Analytical Science and Technology
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    • v.11 no.5
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    • pp.404-408
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    • 1998
  • Using a reversed-phase high performance liquid chromatography, the separation of 20(S)-, 20(R)-prosapogenin stereo-isomers of ginsenoside-$Rg_2$ and of ginsenoside-$Rg_3$ in ginseng saponins has been carried out with binary solvent system. The optimum conditions for the isomer separation are as following: Nova-$Pak^{(R)}C_{18}$ (Waters, $3.9{\times}150mm$) column, $CH_3CN/CH_3CN$ (100:8, v/v) binary solvent system and the flow rate was 1.7 mL/min. The stereoisomers were separated with change of the mixture ratio of the solvent system, the solvent elution by gradient program, and then detected at 203 nm of UV detector. The simultaneous separation of mixture that were the $Rg_2$, $Rg_3$ isomers was easily performed in nonpolar solvent for $Rg_2$, polar solvent for $Rg_3$ at the same optimum conditions.

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CARBON ISOTOPE ANALYSES OF INDIVIDUAL HYDROCARBON MOLECULES IN BITUMINOUS COAL, OIL SHALE, AND MURCHISON METEORITE

  • Kim, Kyoung-Sook;Yang, Jong-Mann
    • Journal of Astronomy and Space Sciences
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    • v.15 no.1
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    • pp.163-174
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    • 1998
  • To study the origin of organic matter in meteorite, terrestrial rocks which contain or-ganic compounds similar to the ones found in carbonaceous chondrites are studied and compared with Muchison meteorite. Hydrocarbon molecules were extracted by benzene and methanol from bituminous coal and oil shale and the extracts were partitioned into aliphatic, aromatic, and polar fractions by silica gel column chromatography. Carbon isotopic ratios in each fractions were analysed by GC-C-IRMS. Molec-ular compound identifications were carried by GC-MS Engine. Bituminous coal and oil shale show the organic compound composition similar to that of meteorite. Oil shale has a wide range of ${\delta}^{13}C,-20.1%_0~-54.4%_0$ compared to bituminous coal, $-25.2%_0~34.3%_0$. Delta values of several molecular compounds in two terrestrial samples are different. They show several distinct distributions in isotopic ratios compared to those of meteorite; Murchison meteorite has a range of ${\delta}^13C\;from\;-13%_0\;to\;+30%_0$. These results provide interpretation for the source and the formation condition of each rock, in particular alteration and migration processes of organic matter. Especially, they show an important clue whether some hydrocarbon molecules observed in meteorite are indigenous or not.

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Isolation and Identification of bakkenolides and caffeoylquinic acids from the aerial parts of Petasites japonicus

  • Woo, Hyun Sim;Lee, Min-Sung;Jeong, Hea Seok;Kim, Dae Wook
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.99-99
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    • 2018
  • The major aim of this work is the research of secondary metabolites isolated from the aerial parts of Petasites japonicus. The plant material is extracted with a polar solvent, which is 95% by volume methanol at room temperature. The concentrated extract was partitioned as EtOAc, n-BuOH, and $H_2O$ fractions. From the EtOAC and n-BuOH fraction, two bakkenolides and two caffoylquinic acid were isolated using the Diaion HP-20, silica gel, ODS-A, and Sephadex LH-20 column chromatographies. According to the results of the results of physico-chemical and spectroscopic data including NMR, MS and UV. The chemical structures of the compounds were respectively determined as bakkenolide B (1), bakkenolide D (2), 1,5-dicaffeoylquinic acid (3), and 5-O-caffeoylquinic acid (4). These results suggest that the compounds isolated from the aerial parts of this plant were almost identical with known components of Petasites japonicus. However, it is necessary to investigate more about the difference of amounts of constituents according to harvest area and time.

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Mesozooplankton Distribution in the Southern Yellow Sea in Autumn (가을철 황해 남부의 중형동물플랑크톤 분포)

  • Kim, Garam;Kang, Hyung-Ku
    • Ocean and Polar Research
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    • v.41 no.4
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    • pp.251-263
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    • 2019
  • We investigated the mesozooplankton community structure during autumn in the southern Yellow Sea. Mesozooplankton density generally ranged from 352 to 2,954 ind. m-3 and varied according to different sampling stations. The copepod Paracalanus parvus s.l. and its copepodites dominated in the communities, corresponding to 57.3% in density of the total. Surface and water-column averaged salinity were positively correlated with density of total mesozooplankton, copepods and a few dominant species, and the tunicate Thalia rhomboides was negatively related to chlorophyll-a concentration. The mesozooplankton community of the study area was divided into three groups according to the cluster analysis using species composition and density: one in the northern coastal region, another in the northern offshore region, and the other in the south. The most significant indicator species for each of the groups were Labidocera euchaeta in the northern coastal region, T. rhomboides in the northern offshore region, and Themisto sp. juveniles in the south. This study provides recent data on the characteristics of the mesozooplankton community in the southern Yellow Sea, which may be valuable for gaining a better understanding of changes in the pelagic ecosystem of the Yellow Sea.

Isolation and Antioxidant Activity of Methyl Aconitates from Arctic Red Alga Polysiphonia stricta (극지 홍조류 Polysiphonia stricta에서 분리된 methyl trans-aconitate 유도체들과 항산화 활성)

  • Lee, Jung Im;Kong, Chang-Suk;Baek, Seung Oh;Seo, Youngwan
    • Ocean and Polar Research
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    • v.36 no.3
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    • pp.247-254
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    • 2014
  • In our continuing study on the antioxidant activity of Polysiphonia stricta, its crude extract was fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions according to solvent polarity. The solvent fractions were evaluated for their potential to inhibit lipid peroxidation and reactive oxygen species (ROS) production in HT 1080 cells. The n-BuOH fraction most strongly inhibited both lipid peroxidation and ROS production in HT 1080 cells. The n-BuOH fraction was further separated by repeated silica gel column chromatography and RP-HPLC to give methyl aconitates (2 and 3). The chemical structure of isolated compounds was determinated by NMR spectral analysis.