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http://dx.doi.org/10.4217/OPR.2014.36.3.247

Isolation and Antioxidant Activity of Methyl Aconitates from Arctic Red Alga Polysiphonia stricta  

Lee, Jung Im (Division of Marine Environment & Bioscience, College of Maritime Sciences Korea Maritime and Ocean University)
Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences Silla University)
Baek, Seung Oh (Division of Marine Environment & Bioscience, College of Maritime Sciences Korea Maritime and Ocean University)
Seo, Youngwan (Division of Marine Environment & Bioscience, College of Maritime Sciences Korea Maritime and Ocean University)
Publication Information
Ocean and Polar Research / v.36, no.3, 2014 , pp. 247-254 More about this Journal
Abstract
In our continuing study on the antioxidant activity of Polysiphonia stricta, its crude extract was fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions according to solvent polarity. The solvent fractions were evaluated for their potential to inhibit lipid peroxidation and reactive oxygen species (ROS) production in HT 1080 cells. The n-BuOH fraction most strongly inhibited both lipid peroxidation and ROS production in HT 1080 cells. The n-BuOH fraction was further separated by repeated silica gel column chromatography and RP-HPLC to give methyl aconitates (2 and 3). The chemical structure of isolated compounds was determinated by NMR spectral analysis.
Keywords
Polysiphonia stricta; methyl aconitate; antioxidant activity;
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1 Misra S, Sanyal T, Sarkar D, Bhattacharya PK, Ghosh DK (1989) Evaluation of antileishmanial activity of transaconitic acid. Biochem Med Metab Biol 42:171-178   DOI
2 Okimoto Y, Watanabe A, Niki E, Yamashita T, Noguchi N (2000) A novel fluorescent probe diphenyl-1-pyrenylphosphine to follow lipid peroxidation in cell membranes. FEBS Lett 474:137-140   DOI   ScienceOn
3 Regoli F, Nigro M, Bompadre S, Winston G (2000) Total oxidant scavenging capacity (TOSC) of microsomal and cytosolic fractions from Antarctic, Arctic and Mediterranean scallops: differentiation between three potent oxidants. Aquat Toxicol 49:13-25   DOI
4 Rémus-Borel W, Menzies JG, Belanger RR (2009) Aconitate and methyl aconitate are modulated by silicon in powdery mildew-infected wheat plants. J Plant Physiol 166:1413-1422   DOI
5 Rémus-Borel W, Shallow N, McNally DJ, Labbe C, Belanger RR (2006) High-speed counter-current chromatography for the study of defense metabolites in wheat Characterization of 5,6-O-methyl trans-aconitic acid. J Chromatogr A 1121:200-208   DOI
6 Seo Y, Lee HJ, Kim YA, Lee JI, Kim JH, Chung H, Kang SH (2007) In vitro peroxynitrite scavenging activity of arctic seaweed extracts. Ocean and Polar Res 29:187-191   과학기술학회마을   DOI
7 Silva ALP, Holmstrup M, Amorim MJB (2013) Worms from the Arctic are better adapted to freezing and high salinity than worms from temperate regions: Oxidative stress responses in Enchytraeus albidus. Comp Biochem Phys A 166:582-589   DOI
8 Xu X, Piggott AM, Yin L, Capon RJ, Song F (2012) Symphyocladins A-G: Bromophenol adducts from a Chinese marine red alga, Symphyocladia latiuscula. Tetrahedron Lett 53:2103-2106   DOI
9 Yanishlieva NV, Marinova E, Pokorny J (2006) Natural antioxidants from herbs and spices. Eur J Lipid Sci Technol 108:776-793   DOI   ScienceOn
10 Zia-ur-Rehman (2006) Citrus peel extract - a natural source of antioxidant. Food Chem 99:450-454   DOI   ScienceOn
11 Chai Y, Niu L, Sun XL, Ding JH, Hu G (2006) Iptakalim protects PC12 cells against $H_2O_2$-induced oxidative injury via opening mitochondrial ATP-sensitive potassium channel. Biochem Biophys Res Commun 350:307-314   DOI
12 Halliwell B (1996) Antioxidants in human health anddisease. Annu Rev Nutr 16:33-50   DOI   ScienceOn
13 Hansen MB, Nielsen SE, Berg K (1989) Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. J Immunol Methods 119:203-221   DOI   ScienceOn
14 Diplock AT, Charleus JL, Crozier-willi G, Kok FJ, Rice-Evans C, Roberfroid M, Stahl W, Vina-Ribes J (1998) Functional food science and defence against reactive oxidative species. Brit J Nutr 80:S77-S112   DOI   ScienceOn
15 Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts. I Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189-198   DOI   ScienceOn
16 Ichiniose M (2003) Inflammatory mechanisms in bronchial asthma and COPD. Tohuku J Exper Med 200:1-6   DOI   ScienceOn
17 Kooy NW, Royall JA, Ischiropoulos H, Beckman JS (1994) Peroxynitrite-mediated oxidation of dihydrorhodamine 123. Free Radic Biol Med 16:149-156   DOI   ScienceOn