• Journal of the korean society of oceanography
• /
• v.38 no.1
• /
• pp.1-10
• /
• 2003

A time-series sediment trap was deployed at a depth of 1034 m in the eastern Bransfield Strait from December 25, 1998 to December 24, 1999. Particle fluxes showed large seasonal variation; about 99% of the annual total mass flux (49 g m/sup -2/) was collected during the austral summer and fall (January-March). Settling particles consisted primarily of biogenic silica, organic carbon, calcium carbonate, and lithogenic material. Biogenic silica and lithogenic material predominated settling particles, comprising 36% and 30% of the total mass flux, respectively, followed by organic carbon, 11% and calcium carbonate, merely 0.6%. The annual organic carbon flux was 5.4 g C m/sup -2/ at 1000 m in the eastern Bransfield Strait, which is greater than the central Strait flux. The relatively lower flux of organic carbon in the central Bransfield Strait may be caused by a stronger surface current in this region. Organic carbon flux estimates in the eastern Bransfield Strait are the highest in the Southern Ocean, perhaps because of the fast sinking of fecal pellets, which leads to less decomposition of organic material in the water column. Approximately 5.8% of the organic carbon produced on the surface in the eastern Bransfield Strait is exported down to 1000 m; this percentage exceeds the maximum EF/sub 1000/ values observed in the Atlantic and Southern Oceans. The eastern Bransfield Strait appears to be the most important site of organic carbon export to the deep sea in the Southern Ocean.

• The Journal of the Petrological Society of Korea
• /
• v.21 no.4
• /
• pp.431-439
• /
• 2012

A column chemistry is the most useful tools for isolating the elements of interest in isotope geochemistry. Here we introduce the chemical experimental procedure for Sm, Nd, La and Ce separation such as Teflon powder or Ln-resin method using HDEHP of KIGAM, KBSI, KOPRI and ${\alpha}$-HIBA(${\alpha}$-Hydroxy Isobutyric acid) method of Nagoya University, Japan. This technical report will provide an useful information in selecting the experiment method for rare earth element isotope system study such as Sm-Nd and La-Ce isotope system.

• Journal of Ginseng Research
• /
• v.13 no.1
• /
• pp.37-41
• /
• 1989

Reactive metabolites generated by benzo(a)pyrene(BP) monooxygenase(AHH) interact with nucleophiles in DNA and cause mutation and carcinogenesis. We studied the effect of Panax ginseng C.A. Meyer, which induce epoxide hydratase(EH) activity without concomitant induction of AHH activity, on the binding of BP metabolites to DNA in uitro in Sprague Dawley rats. DNA-BP metabolite adducts can be resolved into at least five distinct peaks by elution of a Sephadex LH-20 column with a water methanol gradieNt. These peaks are arbitrarily designated A(most polar) through I(least polar). Of the 5 peaks tentatively assigned to 7,8 biol-9,10-oxide(A),7,8·oxide(B),4,5-oxide(C), and further metabolites of 9-OH-BP(D & E), peaks A, C, D, and I were reduced to 70, 85, 80, and 30% of controls, respectively, and there was no significant change in peak B. In connection with this DNA binding study, BP metabolizing enzymes including AHH, EH, demethylase(DM) activity and cyt. P-450 contents were also investigated in order to compare the BP treated control with ginseng and BP treated test groups. The results showed that the EH activity was increased by 139% over the BP control, the Cyt. P-450 content was increased by 180% over the control value, and DM and AHH activities were also increased to some degree for the BP test group, but there was no significant effect of the ginseng treatment.

• Ocean and Polar Research
• /
• v.26 no.4
• /
• pp.635-646
• /
• 2004

Chronic outbreaks of green tide in the Nakdong estuary toll a heavy socioeconomic cost. The paper investigates the influence of sediments on the nitrogen eutrophication, being claimed as the primary cause of green tide. To measure the flux of nitrate at the sediments-water interface, sediment cores were taken in Jan., Mar., May and Sep., 2000 at Noksan located in the West-Nakdong river estuary. The dissolved oxygen was profiled and then the pore water was extracted in situ. Core samples were analyzed for their textural characteristics. Cores were incubated by a novel technique to measure the fluxes of nitrate $(NO_3^-)$ and ammonia $(NH_4^+)$ at the sediment-water interface. The dissolved oxygen was depleted usually within several millimeters in the top sediments. Nitrate started to decrease drastically at the layer where dissolved oxygen was nearly depleted. Nitrate was also exhausted within several centimeters, followed by ammonia build up rapidly. The flux at the sediments-water interface calculated from the pore water concentrations revealed that nitrate was removed from the water column into the sediments. The sediment incubation experiment confirmed the above result. On the other hand ammonia were released from the sediment to the water column. As the incubation went on, however, the nitrate concentration in the overlying water was dropped below that of a top sediment. Then the flux is reversed, i.e., nitrate was released from the sediments to the water column. The implication is that the sediment can supply nitrate to the water column if it falls below a certain level. Thus it is likely that sediments in the eutrophicated river buffers the nitrate concentration in the water column, which leads to a prolonged green tide.

• Korean Journal of Microbiology
• /
• v.45 no.2
• /
• pp.119-125
• /
• 2009

To understand the ecological function of heterotrophic bacterial community in water column of large freshwater lakes in the permafrost zone, we investigated the structure and function of bacterial community in Lake Khuvsgul, Mongolia. Species composition of overall bacterial community was analyzed by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments, and bacteria that can be cultured at 10oC were isolated and characterized. Based on the depth profile of environmental parameters, thermocline and chemocline were recognized at the 5~10 m zone of the water column. The stratified DGGE profile indicated that the discontinuity of water properties might influence the structure of bacterial community: band profiles in the 0~5 m zone were diverse with large change by depth, but the profile was relatively stable at the $\geq$10 m zone, with predominance of the band identified as Acidovorax facilis. Bacterial cultures were screened for protease, cellulase, amylase and lipase activity, and 23 isolates were selected for high activity of the hydrolytic enzymes. The isolates were identified based on their 16S rRNA gene sequences. In the surface water (zero meter depth), Acidovorax defluvii and Sphingobacterium faecium with high cellulase activity were present. Flavobacterium succinicans, Mycoplana bullata and A. facilis were stably predominant isolates at 2 m, 5 m, and $\geq$10 m depths, respectively. F. succinicans isolates showed high protease activity while M. bullata isolates showed moderate levels of protease and celluase activity. A. facilis isolates showed either cellulase or lipase activity, exclusively to each other. According to the profile of growth rates of the isolates in the temperature range of $0\sim42^{\circ}C$, the surface-zone (0~5 m) isolates were facultative psychrophiles while isolates from $\geq$10 m depth were typical mesophiles. This stratification is believed to be due to stratified availability of organic materials to the bacterial decomposers. In the water column below the chemoline, the environment is extremely oligotrophic so that the trait of rapid growth in low temperature might not be demanded by deep-lake decomposers. The stratified distribution of community composition and decomposer activity in Lake Khuvsgul implies that ecological functions of bacterial community in lakes of cold region are sharply divided by water column stratification.

• Ocean and Polar Research
• /
• v.23 no.2
• /
• pp.77-95
• /
• 2001

Temperature, salinity, nutrients, chlorophyll-a, and primary production were measured within the upper 200 m water column in the area around the South Shetland Islands in January, 2000. Surface temperature was relatively high in the Drake Passage north of the South Shetland Islands and low in the northeastern area of the Antarctic Peninsula. In contrast, surface salinity was low in the Drake Passage and increased toward the Antarctic Peninsula, reaching the maximum value in the northeastern area of the Antarctic Peninsula. Surface nutrients were low in the Drake Passage and high in the area near the South Shetland Islands. Surface chlorophyll-a was also low in the Drake Passage and near the Antarctic Peninsula and high in the area of the northern King George Island. The study area could be classified as four geographical zones based on the characteristic shape of the T/S diagrams;the Drake Passage, the Bransfield Strait, the mixed zone, and the Weddell Sea. Each geographical zone showed apparently different physical, chemical, and biological characteristics. Phytoplankton biomass was relatively low in the Drake Passage and the Weddell Sea and high in the Bransfield Strait and the mixed zone. The low phytoplankton biomass in the Weddell Sea could be explained by the low water temperature and deep surface mixing down to 200 m. The high grazing pressure and low availability of iron could be responsible for the low phytoplankton biomass in the Drake Passage.

• Korean Journal of Food Science and Technology
• /
• v.25 no.2
• /
• pp.160-164
• /
• 1993

The antioxidant activity of ethanol extracts from defatted perilla flour was investigated by measuring peroxide value of perilla oil during storage at $45^{\circ}C$. The antioxidant activity of ethanol extracts was also compared with BHA, BHT and tocopherol. Anti-oxidant activity of ethanol extracts was also examined in corn oil and lard. The ethanol extracts contents of defatted perilla flour and the original perilla seed were 7.69 and 4.56% respectively. The antioxidant activity of ethanol extracts was superior to that of 0.02% BHT, BHA and tocopherol in the perilla oil substrate, merely in concentration of one-twentieth as much as that contained in original perilla oil seeds. The fractions of non-polar solvent (hexane and chloroform) obtained from silicic acid column chromatography are less effective than that of polar solvent as an antioxidant. Antioxidant activity of partially purified ethanol fraction is slightly inferior to that of original crude ethanol extracts. Ethanol extracts were also effective in corn oil and lard almost same as in perilla oil. The total phenolic compound contents of crude ethanol extracts and partially purified ethanol fraction were 9.3, 6.4%, respectively.

• Textile Coloration and Finishing
• /
• v.14 no.4
• /
• pp.229-239
• /
• 2002

Colorants were extracted from the heartwood of sappan lin. with MeOH under reflux, and the concentrate or the powder of dye was prepared by low pressure concentration method using suitable organic solvent. Various components were isolated from sappan wood, and the chemical structure and mechanism of compound having the excellent antibacterial and deodorization properties were analyzed. The results obtained are as follows ; The seventeen components of sappan wood were seperated by HPLC chromatography, and the five components among them were existed more than 6% and the other components were existed lower than 0.6%. The resolving powers of the non-polar solvent and polar solvent systems were evaluated by their ability to resolve the samples. It showed that chloroform-methanol-water(800:150:10) system has the best resolving power. Although the seperation rate is very slow, polyamide C-100 column chromatography gives a clear seperation of sappan wood. On the basis of the spectrometric data such as IR, UV, $GC-Mass,\;^1H-NMR,\;^{13}C-NMR\;and\;^1H-^{ 13}C-NMR$, the chemical structure of compound haying the excellent antibacterial and deodorization properties was established as brazilin containing the functional groups such as two quaternary carbon, one benzyl carbon, methylene contiguous to oxygen and methylene caused by oxygen atom.

• Journal of Astronomy and Space Sciences
• /
• v.33 no.4
• /
• pp.305-311
• /
• 2016

The spatial size and variation of Arctic sea ice play an important role in Earth's climate system. These are affected by conditions in the polar atmosphere and Arctic sea temperatures. The Arctic sea ice concentration is calculated from brightness temperature data derived from the Defense Meteorological Satellite program (DMSP) F13 Special Sensor Microwave/Imagers (SSMI) and the DMSP F17 Special Sensor Microwave Imager/Sounder (SSMIS) sensors. Many previous studies point to significant reductions in sea ice and their causes. We investigated the variability of Arctic sea ice using the daily sea ice concentration data from passive microwave observations to identify the sea ice melting regions near the Arctic polar ice cap. We discovered the abnormal melting of the Arctic sea ice near the North Pole during the summer and the winter. This phenomenon is hard to explain only surface air temperature or solar heating as suggested by recent studies. We propose a hypothesis explaining this phenomenon. The heat from the deep sea in Arctic Ocean ridges and/or the hydrothermal vents might be contributing to the melting of Arctic sea ice. This hypothesis could be verified by the observation of warm water column structure below the melting or thinning arctic sea ice through the project such as Coriolis dataset for reanalysis (CORA).

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.20 no.5
• /
• pp.467-472
• /
• 1991

In order to investigate the changes of lipid ingredient and fatty acid content in flint corn by a growth process, total lipids from flint corn(Zea may l.) were extracted, purified and fractionated into three lipid classes (neutral lipid ; NL, glycolipid ; GL, phospolipid ; PL) by silicic acid column chromatography (SACC). Non-polar lipid ingredients and fatty acid contents were determined by thin layer chromatography (TLC) and gas chromatography (GC). And non-polar lipid ingredients were quantitatived by TLC scanner. The crude oil among proximare composition increased 2.74% to 4.43% and NL and PL were fractionated by SACC increased 48.5% to 86.02%, 1.01% to 2.73%, respectively. But GL decreased 50.4% to 11.25%. Neutral lipid ingredients identified monoglyceride, were 1.1~3.7%, 11.9~16.1%, 2.3~4.2%, 16.7~21.5% and 58.5~63.6%, respectively. The major fatty acids of NL were linoleic (48.9~61.0%), oleic(22.3~34.2%), palmitic (10.3~11.7%) acids and stearic, linolenic acids were also detected as minor components. The ratio of total unsaturated fatty acid to total saturated fatty acid presented a higher percentage of 5.87~6.34. The physico-chemical characteristics of flint corn lipids were presented as specific gravity presented 0.920, refractive index 1.468, saponification value 191.2, unsaponificable matter 0.97%, acid value 0.58 and iodine value 124.6, respectively.