• Ocean and Polar Research
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• v.25 no.4
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• pp.645-652
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• 2003

The coastal environment of King George Island is potentially subject to contamination by pollutants arising from station operations, such as emissions from fossil fuel burning, oil spills, waste disposal, etc. As a preparatory step to assess such impacts on the marine environment and living organisms of this island, two molluscan species (the bivalve Laternula elliptica and the gastropod Nacella concinna) were selected as biomonitors for metal pollution monitoring, and their baseline levels have been investigated for the past several years at King Sejong Station. In this review, variability of the baseline levels is discussed in relation to body size, tissue type, and sex. Natural elevations of some metals are also discussed with respect to the environmental characteristics of this region.

• Journal of Embryo Transfer
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• v.22 no.2
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• pp.121-126
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• 2007

The objective of this study was carried out to examine the selection effects of in vitro matured porcine follicular oocytes with polar body extrusion and early cleavage as non-invasive marker to know the developmental competence in advance. The porcine oocytes matured for 48 h were examined the polar body extrusion. The examined oocytes were matured for additional $16{\sim}18h$ and activated with 7% ethanol and cultured in $5{\mu}g/ml$ cytochalasin B for 5 h for diploid formation. The treated oocytes were cultured and examined the cleavage after 48 h and continued culturing for 5 days. The oocytes of 21.9% were discarded in morphological selection and 32.1% oocytes were discarded by failure of first polar body extrusion. The selected oocytes were matured and activated and then after 48 h the cleavage rates were examined. In morphologically selected oocytes, 15.8% oocytes were not cleaved and 52.6% oocytes were normally cleaved and 31.6% oocytes were hyper-cleaved over 8-cell stage. However in the first polar body extruded oocytes, 7.1% oocytes were not cleaved and 73.1% oocytes were normally cleaved and 19.8% oocytes were hyper-cleaved. The morphologically selected embryos that not cleavage-selected were developed in 16.7% up to blastocyst and the morphologically selected and cleavage-selected embryos were developed in 31.7%. The polar body extruded oocytes that were not carried out cleavage selection were developed in 39.0% and the polar body extruded and cleavage-selected embryos were developed 49.0%. The first cleavage timing was examined with 12 h interval after activation. In $0{\sim}12,\;12{\sim}24,\;24{\sim}36,\;and\;36{\sim}48h$ intervals, 4.1%, 68.6%, 19.1%, and 2.3% oocytes were cleaved and 5.9% oocytes were not cleaved until 48 after activation. The cleaved oocytes in each interval were cultured and developed upto blastocyst with 0, 39.1, 9.5, and 0%, respectively. This results suggests that polar body extruded and cleaved at $12{\sim}36h$ embryo has higher developmental potential than the others.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.35 no.2
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• pp.165-170
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• 2007

During the conceptual design phase of a wing-body-canard type fighter class aircraft, as a method of maneuverability enhancement for an aircraft, effects of canard-leading edge flap scheduling have been studied. In this study, corrected supersonic panel method has been used to predict the drag polar characteristics due to canard-leading edge flap deflections in the high speed regime. Utilizing the predicted drag polar curves, the canard-leading edge flap scheduling laws have been established. These scheduling laws are the relation of canard-leading edge flap deflections and the flight conditions to maximize the lift-drag ratio. Based on the results obtained from the canard-leading edge flap scheduling, the present method has shown to be useful to enhance the maneuverability of wing-body-canard type fighter class aircraft.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.58 no.4
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• pp.345-351
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• 2022

In this study, the target strength for multi-frequency (38 kHz, 120 kHz, 200 kHz) of juvenile silverfish (Pleuragramma antarcticum) was estimated using by the KRM (Kirchhoff-ray mode) model. The body shape of the silverfish was described by a picture and the body length of nine individuals ranged in 1.8 cm to 8.8 cm. The maximum TS_cm according to the total length for the constant term (b20) was -92.93 dB at 38 kHz, -86.63 dB at 120 kHz, and -85.89 dB at 200 kHz, respectively. The averaged TS_cm according to total length for the constant term (b20) was -100.0 dB at 38 kHz, -93.0 dB at 120 kHz, and -106.9 dB at 200 kHz, respectively.

• Journal of Astronomy and Space Sciences
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• v.27 no.2
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• pp.97-106
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• 2010

To prepare for a Korean lunar orbiter mission, a precise lunar orbit propagator; Yonsei precise lunar orbit propagator (YSPLOP) is developed. In the propagator, accelerations due to the Moon's non-spherical gravity, the point masses of the Earth, Moon, Sun, Mars, Jupiter and also, solar radiation pressures can be included. The developed propagator's performance is validated and propagation errors between YSPOLP and STK/Astrogator are found to have about maximum 4-m, in along-track direction during 30 days (Earth's time) of propagation. Also, it is found that the lifetime of a lunar polar orbiter is strongly affected by the different degrees and orders of the lunar gravity model, by a third body's gravitational attractions (especially the Earth), and by the different orbital inclinations. The reliable lifetime of circular lunar polar orbiter at about 100 km altitude is estimated to have about 160 days (Earth's time). However, to estimate the reasonable lifetime of circular lunar polar orbiter at about 100 km altitude, it is strongly recommended to consider at least $50\;{\times}\;50$ degrees and orders of the lunar gravity field. The results provided in this paper are expected to make further progress in the design fields of Korea's lunar orbiter missions.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2006.10a
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• pp.122-123
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• 2006

• 대한생식의학회:학술대회논문집
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• 2002.11a
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• pp.80-80
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• 2002

• Journal of Embryo Transfer
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• v.29 no.2
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• pp.111-117
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• 2014

This study was conducted to optimize the efficiency of cloning and to produce cloned mice. The majority of cloned mammals derived by nuclear transfer (NT) die during gestation and have enlarged and dysfunctional placentas. In this study, the optimized conditions were established to produce clone mice. The parthenogenetic oocytes were activated after 6 h regardless of cytochalasin B (CB) concentration. CB treatment ($2{\mu}g/ml$) was found second polar body. Lower concentration of CB was decreased the activation rate, but the second polar body was the best highly increased during 6 h incubation. The small fragments were exhibited in the $5{\mu}g/ml$ treatment of CB, but it was not found in lower concentration groups (> $2.5{\mu}g/ml$). To examine effects of $SrCl_2$ on the adult cumulus cells, somatic cell NT oocytes were exposed during 0.5, 1 and 6 hrs. The second polar body was significantly greater in 0.5 h exposure group (6.6%) than 1, 6 hrs. Developmental rate from 2-cell to 4-cell was the lowest in 7.5 mM Strontium chloride ($SrCl_2$) groups (84.1% and 64.3%) than 5, 10 m $MSrCl_2$. The implantation rate was not significantly difference among 5, 7.5 and 10 m $MSrCl_2$ group. Three live fetuses were produced by SCNT. SCNT placentas were remarkably heavier than IVF group (8 fetuses) (0.34, 0.34, 0.33 vs 0.14 g) compared with the placenta weight of IVF and SCNT clones.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.79-79
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• 2002

본 연구에서는 in vitro에서 성숙된 난자의 핵성숙(Polar Body extrusion)에 소요되는 시간과 배반포 단계로의 발달능력 사이의 관계를 비교하여 조기에 발달능력을 가진 embryo를 선발할 수 있는 IVP 체계를 개발하고자 하였으며 in vitro maturation(IVM)에 따른 first polar body(PB) 형성, IVM과 IVF 시간이 oocyte의 발달에 미치는 영향과 생산된 배반포의 세포수를 평가하였다. IVM은 TCM199 배양액을 사용하였고 in vitro fertilization(IVF)은 Fer -TALP용액을 사용하였으며 in vitro culture(IVC)는 CRlaa 배양액을 사용하여 2일까지는 0.3% BSA를 3일 부터는 10%FBS와 bovine oviduct epithelial cell을 첨가하여 배양하였다. IVM 시간에 따른 PB의 출현율은 0hr(0%), 6hr(0%), 12hr(0%), 14hr(8.7%), 16hr(40.5%), 18hr(48.0%), 20hr(65%), 22(68%) 그리고 24hr(74.5%)을 보였으며 IVM 시간에 따른 cleavage 및 8cell 발달율 사이에는 유의적인 차이가 없었으나 배반포(BL) 및 8cell에서 배반포로 발달률은 18시간(BL 31$\pm$6, BL/8cell 82 $\pm$5%)에서 가장 높게 나타났으며 24시간(BL 17$\pm$2, BL/8cell 60$\pm$8%)과 유의적인 차이를 보였다(P<0.05). IVC 7일째 배반포의 총세포수와 trophoblast(TE) 세포수는 IVM 18시간(mean$\pm$S.E.; total: 131.1$\pm$34.0, TE: 97.6$\pm$29.6)에서 24시간(total: 112.2$\pm$17.5, TE: 80.1$\pm$15.6)보다 유의하게 많은 것으로 나왔으나(P<0.05) 7일째의 inner cell mass(ICM) 숫자(18hr 33.5$\pm$12.8 vs 24hr 32.1$\pm$12.0)와 8일째 ICM, TE 그리고 총 세포수에는 유의성 있는 차이가 없었다. IVM 18시간에서 PB 형성과 8cell 발달률 사이에 높은 상관성을 보였고 배반포 및 8cell에서 배반포 단계로 높은 발달률을 보였으며 생산된 배반포의 TE 숫자와 총 세포수가 유의하게 많은 것으로 나타났다. 따라서 IVM 18시간 실시하였을 경우 보다 많은 세포수를 가진 배반포 발달 가능성이 높은 embryo를 조기에 선발 가능할 것으로 사료된다.

• Journal of Embryo Transfer
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• v.13 no.3
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• pp.219-226
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• 1998

Enucleation of oocytes is an important limiting step for embryo cloning. We propose an enucleation technique based on the removal of chromatin after oocyte activation by aspirating the second polar body containing complemented chromatin. In a preliminary experiment to determine an optimal age of oocytes enucleation in rabbits, oocytes were enucleated at 15~20 hours post hCG. Recently ovulated oocytes were enucleated at a higher rate than aged oocytes. Microsurgical removal of the complemented chromatin in the second polar body was significantly more effective in enucleating than aspiration of a larger cytoplasm volume surrounding the first polar body of metaphase-arrested oocytes(96.8% versus 70.4%; P〈0.05). Moreover, compared with a nuclear transplantation protocol based on enucleation of metaphase-arrested oocytes and preactivated oocytes followed by treatment with 5 $\mu$M ionomycin for 5 min and 2 mM DMAP for 1 hr, there was no significant difference in the rate of blastocyst development. The ease with which modified technique can be performed is likely to render this technique widely useful for research and practice on mammalian cloning.