• Journal of Periodontal and Implant Science
• /
• 제37권1호
• /
• pp.11-21
• /
• 2007

Heat shock protein (HSP) is one of cellular protein commonly present in major periodontopathogenic bacteria as well as mammalian cells. The protein may play a role in the immunopathogenesis by modulating autoimmune reaction due to its high level of sequence homology between bacteria and human counterpart. Hence, identifying immunodomiant epitope of bacteria HSP that is cross-reactive to periodontopathogenic bacteria with a specificity to human HSP may comprise a critical strategy for development of a periodontal vaccine. The present study was performed to establish clones producing monoclonal antibody reactive to Porphyromonas gingivalis (p. gingivalis) HSP with a specificity to human HSP. 4 different hybridomas were cloned producing monoclonal IgG antibodies to P, gingivalis HSP and evaluated for their reactivity and specificity to other periodontopathogenic bacteria as well as to human HSP. These four monoclonal antibodies reacted with p. gingivalis HSP only with specificities to other bacteria tested and human HSP as well. The antigenic epitopes producing the 4 monoclonal antibody may be potentially developed as vaccine candidates. Further investigations are under way to identify more clones producing monoclonal antibodies reactive to P, gingivalis HSP and to other periodontopathogenic bacteria as well, while maintaining specificities to human counterpart.

• BMB Reports
• /
• 제41권4호
• /
• pp.278-286
• /
• 2008

Angiogenesis, the formation of blood vessels, is essential for preparing a closed circulatory system in the body, and for supplying oxygen and nutrition to tissues. Major diseases such as cancer, rheumatoid arthritis, and atherosclerosis include pathological angiogenesis in their malignant processes, suggesting anti-angiogenic therapy to be a new strategy for suppression of diseases. However, until the 1970s, the molecular basis of angiogenesis was largely unknown. In recent decades, extensive studies have revealed a variety of angiogenic factors and their receptors, including vascular endothelial growth factor (VEGF)-VEGFRs, Angiopoietin-Tie, Ephrin-EphRs and Delta-Notch to be the major regulators of angiogenesis in vertebrates. VEGF and its receptors play a central role in physiological as well as pathological angiogenesis, and functional inhibitors of VEGF and VEGFRs such as anti-VEGF neutralizing antibody and small molecules that block the tyrosine kinase activity of VEGFRs have recently been approved for use to treat patients with colorectal, lung, renal and liver cancers. These drugs have opened a novel field of cancer therapy, i.e. anti-angiogenesis therapy. However, as yet they cannot completely cure patients, and cancer cells could become resistant to these drugs. Thus, it is important to understand further the molecular mechanisms underlying not only VEGF-VEGFR signaling but also the VEGF-independent regulation of angiogenesis, and to learn how to improve anti-angiogenesis therapy.

• 한국의류산업학회지
• /
• 제5권5호
• /
• pp.503-510
• /
• 2003

The purpose this study is to segments apparel market based on difference real-self image and ideal-self image. The objects of the study were to prepare for the establishment of marketing strategy and alternative plan intended to users which are needed in subdivided market, after analyzing according to what the subdivided market is divided into due to the difference real-self image and idea-self image and what difference do they show as a demographic special quality or as a general active special quality in each subdivided market. Factor analysis was performed to determine the leading difference real-self image and ideal-self image, and cluster analysis was employed to identify groups of respondents based on the delineated five image difference factors. Based on the finding, three distinct groups were formed: ideal-self image seeker group, moderators group, real-self image seeker group. And logistic regression was used to assess the relative importance that demographic characteristics play in determining the segmentation. The results of this study show statistically significant differences among the three groups in terms of demographic. Marketing and management implications for effectively targeting the segments are discussed.

• BMB Reports
• /
• 제39권5호
• /
• pp.479-491
• /
• 2006

Heme oxygenase (HO), the rate limiting enzyme in the breakdown of heme into carbon monoxide (CO), iron and bilirubin, has recently received overwhelming research attention. To date three mammalian HO isozymes have been identified, and the only inducible form is HO-1 while HO-2 and HO-3 are constitutively expressed. Advances in unveiling signal transduction network indicate that a battery of redox-sensitive transcription factors, such as activator protein-1 (AP-1), nuclear factor-kappa B (NF-${\kappa}B$) and nuclear factor E2-related factor-2 (Nrf2), and their upstream kinases including mitogen-activated protein kinases play an important regulatory role in HO-1 gene induction. The products of the HO-catalyzed reaction, particularly CO and biliverdin/bilirubin have been shown to exert protective effects in several organs against oxidative and other noxious stimuli. In this context, it is interesting to note that induction of HO-1 expression contributes to protection against liver damage induced by several chemical compounds such as acetaminophen, carbon tetrachloride and heavy metals, suggesting HO-1 induction as an important cellular endeavor for hepatoprotection. The focus of this review is on the significance of targeted induction of HO-1 as a potential therapeutic strategy to protect against chemically-induced liver injury as well as hepatocarcinogenesis.

• 의학교육논단
• /
• 제17권2호
• /
• pp.57-59
• /
• 2015

Student research has been proposed as an educational strategy to fulfill the current requirements in basic medical education (BME) and to compatible with the self-directed development of professionalism. It is commonly accepted that the goals of student research are to develop the competencies of critical, reflective, and self-directed thinking; problem-solving; and creativity; as well as to acquire the skills necessary to search for information and analyze the literature; to cultivate the talent of mastering a specialized field through deeply intensified learning; and to establish close relationships between students and supervisors. To successfully implement student research, authorities on BME should to plan the procedure for the student research projects and allocate personal and material resources adequately in order to provide the opportunity for self-evaluation and reflection through the completion of daily records, to develop the habit of consistently evaluating one's own study, and to maintain a collegial relationship between students and supervisors by offering the proper feedbacks in a timely and consistent manner. In conclusion, despite several obstacles and difficulties in the establishment of successful student research projects, student research could provide students the motivation to develop themselves into expert academic researchers, and play a role in educating students to help solve patients' problems based on scientific evidence in the future.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권12호
• /
• pp.1837-1841
• /
• 2003

In an attempt to isolate novel molecules that may play a regulatory role in adipocyte differentiation, we devised an experimental strategy to identify adipose tissue-specific genes by modifying cDNA microarray technique. We used genefilter membranes containing approximately 15,000 rat non-redundant EST clones of which 4,000 EST were representative clones of known genes and 11,000 ESTs were uncharacterized clones. A series of hybridization of genefilter membranes with cDNA probes prepared from various rat tissues and nucleic acids sequence analysis allowed us to identify two adipose-tissue specific genes, adipocyte-specific secretory factor (ADSF) and H-rev107. Verification of tissue-specific expression patterns of these two genes by Northern blot analysis showed that ADSF mRNA is exclusive expressed in adipose tissue and the H-rev107 mRNA is predominantly expressed in adipose tissue. Further analysis of gene expression of ADSF and H-rev107 during 3T3-L1 adipocyte differentiation revealed that the ADSF and H-rev107 gene expression patterns are closely associated with the adipocyte differentiation program, indicating their possible role in the regulation of adipose tissue development. Overall, we demonstrated an application of modified cDNA microarray technique in molecular cloning, resulting in identification of two novel adipose tissue-specific genes. This technique will also be used as a useful tool in identifying novel genes expressed in a tissue-specific manner.

• 한국도로학회논문집
• /
• 제16권4호
• /
• pp.127-134
• /
• 2014

PURPOSES : To operate more efficient traffic management system, it is utmost important to detect the change in congestion level on a freeway segment rapidly and reliably. This study aims to develop classification method of congestion change type. METHODS: This research proposes two classification methods to capture the change of the congestion level on freeway segments using the dedicated short range communication (DSRC) data and the vehicle detection system (VDS) data. For developing the classification methods, the decision tree models were employed in which the independent variable is the change in congestion level and the covariates are the DSRC and VDS data collected from the freeway segments in Korea. RESULTS : The comparison results show that the decision tree model with DSRC data are better than the decision tree model with VDS data. Specifically, the decision tree model using DSRC data with better fits show approximately 95% accuracies. CONCLUSIONS : It is expected that the congestion change type classified using the decision tree models could play an important role in future freeway traffic management strategy.

• 한국유기농업학회:학술대회논문집
• /
• 한국유기농업학회 1999년도 하반기 심포지엄
• /
• pp.5-24
• /
• 1999

There are severl types of Eco-village developed in abvanced countries including urban, suburban and rural style. For the establishment of Eco-village in Korea, it was focussed to introduce the rural style which has been developed inyersively in European countries such as Denmark, England and Germany. As a key cencept for Eco-villge it was explained briefly and discussed on the renewable energy(wind power park, bio-mass and solar energy collector and heating system), eco-architure, methane gas device, living machine (wastewater treatment facility) and organic farming. It was also discussed how important the basic standard and guidelines for organic agriculture to run essential for the system. For this reason major principles of international applicable standard(draft) for organically grown foods by FAO/WHO Codex alimentarius was shortly desidered. as one of very suitable type for Environmental Agriculture Districts Program which Korean govenment is going to establish in the near future. In conculusion it was also suggested that positive participation of organic famers, enthusiastic eneagement of inhabitans and support strategy of govemment /NGO groups might play very important role for successful managment of Eco-village ecologically sustainable after establishment.

• 한국임상약학회지
• /
• 제30권3호
• /
• pp.143-148
• /
• 2020

Objective: This study aimed to investigate pharmaceutical care for critically ill neonates and suggest targeted strategies compatible with the Korean health-system pharmacy. Methods: Articles that reported pharmacy practices for critically ill neonates were reviewed. Pharmaceutical care practices and roles of neonatal pharmacists were identified, and criteria were developed for neonates in need of specialized care by clinical pharmacists. Results: Neonatal pharmacists play many roles in the overall medication management pathway. For clinical decision support, multidisciplinary ward rounds, clinical pharmacokinetic services, and consultation for pharmacotherapy and nutrition support were conducted. Prevention and resolution of drug-related problems through review of medication charts contributed to medication safety. Pharmaceutical optimization of intravenous medication played an important role in safe and effective therapy. Information on the use of off-label medicine, recommended dosage and dosing schedules, and stability of intravenous medicine was provided to other health professionals. Most clinical practices for neonates in Korea included therapeutic drug monitoring and nutrition support services. Reduction in medication errors and adverse drug reactions, shortening the duration of weaning medicines, decreasing the use and cost of antimicrobials, and improvement in nutrition status were reported as the outcomes of pharmacist-led interventions. The essential criteria of pharmaceutical care, including for patients with potential high-risk factors for drug-related problems, was developed. Conclusion: Pharmaceutical care for critically ill neonates varies widely. Development and provision of standardized pharmaceutical care for Korean neonates and a stepwise strategy for the expansion of clinical pharmacy services are required.

• Earthquakes and Structures
• /
• 제18권1호
• /
• pp.129-145
• /
• 2020

Seismic resilience is a key feature for buildings that play a strategic role within the community. In this framework, not only the structural and non-structural elements damage but also the protracted structural dysfunction can contribute significantly to overall seismic damage and post-seismic crisis situations. Reduction of the residual and peak displacements and energy dissipation by replaceable elements are some effective aspects to pursue in order to enhance the resilience. Control systems able to adapt their response based on the nature of events, such as active or semi-active, can achieve the best results, but also require higher costs and their complexity jeopardizes their reliability; on the other hand, a passive control system is not able to adapt but its functioning is more reliable and characterized by lower costs. In this study it is proposed a strategy for the optimization of the dissipative capacity of a seismic resistant system obtained placing in parallel two different groups dissipative Re-Centering Devices, specifically designed to enhance the energy dissipation, one for the low and the other for the high intensity earthquakes. In this way the efficiency of the system in dissipating the seismic energy is kept less sensitive to the seismic intensity compared to the case of only one group of dissipative devices.