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Screening of Bifidobacterium spp. for the Development of Infant Probiotics (유아용 생균제 개발을 위한 Bifidobacterium spp.의 선발)

  • Yang, Hyun-Ju;Jang, Keum-Il;Kim, Chung-Ho;Lee, Yoon-Bok;Sohn, Heon-Soo;Kim, Kwang-Yup
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.790-794
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    • 2004
  • Bifidobacterium spp. exhibits the highest number of counts among species of microflora in breast-feeding infant intestines and has been used as probiotics. From infant groups with different diets, 42 Bifidobacterial strains were isolated by selective plate, Gram-staining, and morphology using method of Mitsuoka, among which seven isolates were identified as Bifidobacterium spp. by F6PPK test, MIDI, and PCR. B. bifidum PBH-30, selected for development of probiotics, showed high resistance against low pH and oxgall treatment, and inhibition against pathogens such as Salmonella typhimurium and Staphylococcus aureus. B. bifidum PBH-30 could be applicable to dairy products as probiotic strains due to its excellent growth in raw milk.

Lateral-Torsional Buckling Strength of I-girder with Corrugated Steel Webs under Linear Moment Gradient (선형 모멘트 구배가 작용하는 파형강판 I-거더의횡-비틂 좌굴 강도)

  • Moon, Jiho;Lim, Nam-Hyoung;Lee, Hak-Eun
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.32 no.3A
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    • pp.149-160
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    • 2012
  • Corrugated steel plates have several advantages such as high resistance for shear without stiffeners, minimization of welding process, and high fatigue resistance. To take advantage of these benefits, several researchers have attempted to use corrugated steel plate as a web of I-girders. The lateral-torsional buckling is the major design aspect of such I-girders. However, lateral-torsional buckling of the I-girder with corrugated steel webs still needs to be investigated especially for a real loading condition such as non-uniform bending. This paper investigated the lateral-torsional buckling strength of the I-girder with corrugated steel webs under linear moment gradient by using finite element analysis. From the results, it was found that the buckling behavior of the I-girder with corrugated steel webs differed depending on the number of periods of the corrugation. Also, a simple equation for the moment gradient correction factor of the I-girder with corrugated steel webs was suggested. The inelastic lateral-torsional buckling strength of the I-girder with corrugated steel webs was then discussed based on current design equations for ordinary I-girders and the results of finite element analysis.

A Study on the Change of Streptococcus Mutans in Dental Plaque after Use of $0.05\%$ NaF in Orthodontic Patients (NaF $0.05\%$ 양치액 사용시 고정성 교정장치 장착 환자에서의 Streptococcus mutans 변화에 관한 연구)

  • Hwang, Chung-Ju;Lim, Seon-A
    • The korean journal of orthodontics
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    • v.27 no.4 s.63
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    • pp.539-548
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    • 1997
  • The purpose of this study was to evaluate the effectiveness of gargling solution with 0.05% NaF and 10% Xylitol in orthodontic patients with fixed appliance. The sample consisted of 20 patients who were classified into an experimental group and a control group, 10 patients each. Experimental group was used experimental gargling solution and the control group was used with placebo solution. The change of S. mutans was analysed by culture on MSB and BHI agar plate pre and post 3, 6, 9 weeks. The results were as follows. 1 There were significant reduction in the number of S. mutans C.F.U. between pre and post 3 weeks(p<0.01), 9 weeks(p<0.05) in experimental group 2. There were significant reduction in the ratio of S. mutans C.F.U. to total C.F.U. between pre and post 3, 6, 9 weeks(p<0.01) in experimental group. 3. S. mutans, which were reduced until 3 weeks, did not show significant change after 3, 6, 9 weeks. 4. S. mutans were strongly suppressed until 3 weeks after gargling solution with 0.05% NaF and 10% Xylitol.

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Quality Characteristics of Fresh Noodles With Hot-air-dried Perilla Leaf powder (열풍 건조 들깻잎 분말을 첨가한 생면의 품질특성)

  • Kim, Jung-Soo;Ahn, Jong-Sung;Ahn, Kwang-Yeol
    • Culinary science and hospitality research
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    • v.19 no.3
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    • pp.73-86
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    • 2013
  • This study produced hot-air-dried perilla leaf to add Korea's popular perilla leaf to fresh noodles to examine their value as food and get the best fresh noodle recipe by adding 0%, 3%. 6%, 9%, and 12% of perilla leaf to flour. The moisture content of the dough was highest at 34.41% for the control group with 0% perilla leaf, whereas the pH was lowest at 5.59 for the dough with 12% perilla leaf. As a result of WRC analysis of the dough, moisture absorption increased with greater perilla leaf content. The moisture content of fresh noodles was lowest for the control group with 0% perilla leaf, whereas the pH was highest for the control group. The L, a, and b values of the dough were highest for the group with 0% perilla leaf powder. Hardness and cohesiveness were lowest for the 0% group and springiness tended to decrease with increased amounts of hot air-dried perilla leaf. The number of microorganisms decreased significantly with greater perilla leaf content on the third and fourth days. As a result of the sensory test, the 6% dough showed the highest scores for all items including appearance, flavor, color, taste, and texture. Overall acceptability was also highest at 7.20 for the 6% dough and lowest at 3.27 for the 12% dough. Based on the above results, when producing fresh noodles by adding hot-air-dried perilla leaf powder, inhibition of microorganisms improved with greater perilla leaf content for longer storage, but the sensory properties were best when 6% perilla leaf content was added.

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Planting Design in Green Open Space, Urban Area : Planting Evaluation of Buffer Green Space in Housing Complex (도시지역 녹화공간의 배식기법 : 공동주택단지 완충녹지의 배식)

  • Cho, Woo
    • Korean Journal of Environment and Ecology
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    • v.12 no.1
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    • pp.78-90
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    • 1998
  • An objective of this study was to provide database for the planting disign of buffer green space. Types, planting structure, and effect of vuffer green space were investigated in five housing complexes of newtown of metropolitan area, Korea. Buffer green space in the study sites were constructed as mounding, slope, and plate. The number of species was found 20 tree and sub-tree species(10 evergreen and 20 deciduous species ) and 13 shrub species. These species were planted in one-storyed planting structure and there was no difference with ornamental species in the urban parks. Effect of sound proof by the buffer green space was recognized but sound level in four types among the seven types was observed above standard sound level for housing complex(65dB). Effect of sound proof was especially most effective in the mounding type. It was found that planting density and index of plant crown volume were mot satisfied to the function of buffer green space because of lower density and crown volume than natural vegetation per unit. Based on these results, this study suggested that buffer green space is desirable to be developed in the mounding type over two meters height with multi-layer planting model. In addition, there is needed to consider vegetation structure of natural forest around the developing site.

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Grid Tests for Large Eddy Simulation of Transitional Flows around Turbulence Stimulators (난류 촉진기 주위 천이 유동의 대형 와 모사를 위한 격자 테스트)

  • Lee, Sang Bong;Park, Dong Woo;Paik, Kwang-Jun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.1
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    • pp.112-121
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    • 2017
  • Large eddy simulations of transitional flows around a stud installed on a flat plate have been performed to investigate an influence of grid resolution on turbulence stimulation by the stud. Because streamwise vortical structures generated by the stud played an important role in turbulence stimulation of boundary layer, streamwise vorticity was compared in the wake region behind the stud when the number of grids increased or decreased by a ratio of ${\sqrt{2}}$ in streamwise, wall-normal and spanwise directions respectively. The streamwise vorticity was shown to be mainly affected by spanwise grid resolution (${\Delta}z^+$) rather than streamwise and wall-normal grid resolution. In a viewpoint of numerical efficiency as well as physical resolution, ${\Delta}x^+{_{min}}=7.6$, ${\Delta}x^+{_{max}}=41$, ${\Delta}y^+{_{wall}}=0.25$ and ${\Delta}z^+=7.6$ was found to be desirable. Once a grid resolution was determined in each direction, a grid verification was carried out by increasing or decreasing the grid resolution y a ratio of ${\sqrt{2}}$ in all directions. The grid uncertainties of pressure and drag coefficients were 21.6 % and 2.8 % while the corrected uncertainties were 2 % and 0.3 %, respectively.

THE EFFECTS OF BARIUM SULFATE AND IODIDE COMPOUND ON THE CHARACTERISTICS OF DENTAL ACRYLIC RESINS (치과용 아크릴릭 레진의 방사선 불투과도에 관한 연구 - 황산바륨과 요오드 화합물 첨가 -)

  • Lee Yong-Keun;Lee Keon-Il;Jung Sung-Woo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.26 no.2
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    • pp.133-145
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    • 1996
  • Aspirating or swallowing foreign bodies is a common occurrence. If they are wholly or partly radiopaque, their localization in and progress through the gastrointestinal tract can be more effective. Of the dental origin foreign materials swallowed, the most common things are fragments of anterior maxillary partial denture. But the radiopacity of denture base resins is not sufficient to determine the location of the objects. The purpose of this study was to develop a radiopaque dental acrylic resin, which has clinically detectible radiopacity with minimal change of mechanical properties and color. The radiopacity, color change(CIE 6..E) and microhardness of acrylic resins were determined after mixing barium sulfate or iodide compound. Thermocycling course was conducted to determine the change of characteristic of resins after using for a long time in the mouth. Five or ten percent of barium sulfate to total weight of cured material was mixed with heat curing dental acrylic resin or chemically curing orthodontic resin. In the case of iodide compound, the mixing ratio was two or three percent. After mixing the high radiopaque materials, resin was cured to 20×20×2 mm plate, polished with #600 sand paper and finally polished with Microcloth(Buehler). The specimens were thermocycled in 5 and 55 t distilled water for 2,000 times, and the measurement of radiopacity, color and Vickers hardness was repeated every 500 times thcrmocycling. The radiopacity of specimens on the X -ray films was measured with densitometer(X-rite). The color change was detennined with differential colorimeter(Model TC-6FX, Tokyo Denshoku), and the Vickers hardness number was measured with microhardness tester(Mitsuzawa). The following results were obtained : 1. All the three variables, the kinds of acrylic resins, the mixing or the kinds of high radiopaque materials and thermocycling, had combined effect on the radiopacity of the dental acrylic resins(p<0.0l). 2. The two variables, the mixing or the kinds of high radiopaque materials and thermocycling, influenced on the radiopacity of the dental acrylic resins(p<0.01). But the kinds of acrylic resins did not influence on the color change of mixed dental acrylic resins(p>0.05). 3. Each of the three variables, the kinds of acrylic resins, the mixing or the kinds of high radiopaque materials and thermocycling, influenced on the radiopacity of dental acrylic resins(p<0.0l). 4. The high radiopaque materials used in this study did not yield clinically usable radiopacity, and the color change was great after mixing those materials.

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Phylogenetic diversity of bacterial communities in a gray solar saltern and isolation of extremely halophilic bacteria using culturomics (토판염전 결정지 내 세균군집의 계통학적 다양성 및 Culturomics법을 이용한 고도 호염균의 분리)

  • Cho, Geon-Yeong;Han, Song-Ih;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.53 no.1
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    • pp.29-38
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    • 2017
  • In this study, we investigated the phylogenetic diversity of the bacterial community and isolation of extremely halophilic bacteria using culturomics in a gray solar saltern. The number of bacterial living cells, enumerated in a gray solar saltern by direct fluorescence microscopy was three to four orders of magnitude greater than those enumerated by plate counts, suggesting the distribution of 'viable but non-culturable bacteria'. The biodiversity of bacterial communities in a gray solar saltern was investigated by pyrosequencing, 1,778 OTUs of bacteria were comprised of 18 phyla 46 classes 85 orders 140 families 243 genera with 6.16 diversity index. Archaea communities were composed of 3 phyla 6 classes 7 orders 7 families 38 genera with 4.95 diversity index from 643 OTUs. Totally 137 isolates were isolated by 59 different cultural methods based on culturomics considering culture media and conditions suitable for the growth of extremely halophilic bacteria. Phylogenetic analyses of extremely halophilic isolates based on 16S rRNA gene sequences, extremely halophilic isolates were composed of 4 phyla and 11 genera. Haloterrigena and Haloferax can be successfully isolated from culturomics. These culturomics were effective methods for collection of diversity of extremely halophilic bacteria.

Experimental and FEMLAB Simulation Study of Ibuprofen Racemate Separation in HPLC (Ibuprofen Racemate의 HPLC 분리실험과 FEMLAB 전산모사 연구)

  • Lee, Eun;Chang, Sang-Mork;Kim, Jong-Min;Kim, Woo-Shick;Kim, In-Ho
    • KSBB Journal
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    • v.21 no.3
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    • pp.224-229
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    • 2006
  • FEMLAB is a powerful interactive environment for modeling, solving all kinds of scientific and engineering problems based on partial differential equations(PDEs). Separation process of chiral compound in HPLC columns was simulated by FEMLAB. To study change of elution profile with isotherm models, non-competitive and competitive Langmuir adsorption isotherm were adopted. Separated material was (R, S)-ibuprofen [(R, S)-2-(4-isobutyl phenyl) propionic acid], an anti-inflammatory agent, which retain the pharmacological activity in the (S)-(+)-enantiomer. Sample concentrations were changed from 0.5 mg/ml to 2.0 mg/ml at a flow rate of 1 ml/min and flow rate varied from 1 ml/min to 3 ml/min at an ibuprofen concentration of 2.0 mg/ml and $20{\mu}l$ of injection volume. Simulated results were well fitted with experimental data.

An Evaluation on the Shear Strength of New Type Shear Connectors for a Simple Steel-Concrete Composite Deck (초간편 강합성 바닥판 신형식 전단연결재의 전단내력 평가)

  • Yoon, Ki Yong;Kim, Sang Seup;Han, Deuk Cheon
    • Journal of Korean Society of Steel Construction
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    • v.20 no.4
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    • pp.519-528
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    • 2008
  • A simple steel-concrete composite deck is developed for preventing the lateral torsional buckling of girders that are under construction and for reducing the term of works using H-shaped rolled beams as bridge girders. A new type of shear connectors is also developed for the composite behavior between a simple steel-concrete composite deck and the rolled beams by the connecting conditions between the deck and the girders. One is a connector bolt that is lengthened and split or tightened with two nuts and the other is an I-shaped rolled beam welded on a steel plate with a number of holes punched through the web. In this study, to estimate the shear strength of those shear connectors the push-out tests are performed and the test results are compared with that of the previous studies and the codes. The result of the push-out tests of the connector bolts showed that the shear performance is similar to that of the stud connector and revealed that the equation for the shear strength in the Korean Specification of Highway Bridge overestimates the shear capacity of the connector bolt whose diameter is larger than 19mm. From the push-out tests of punched I-shaped rolled beams with varying welding amounts, with the small amount of welding, shear capacity is governed by the shear capacity of welding. On the other hand, shear capacity is governed by the size of the punched I-shaped rolled beams, regardless of the amount of welding.