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Comparison of Water Potential Parameters in Aster scaber and Synurus deltoides Leaves Obtained from P-V Curves (P-V 곡선법에 의한 참취와 수리취의 수분포텐셜 비교)

  • Lee, Kyeong-Cheol;Jeon, Seong-Ryeol;Han, Sang-Sup
    • Korean Journal of Plant Resources
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    • v.24 no.4
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    • pp.413-418
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    • 2011
  • This study was carried out to establish a proper cultivation site and diagnose the drought-tolerance of Aster scaber and Synurus deltoides leaves by using Pressure-volume curves. In order to measure pressure-volume (P-V) curves, Aster scaber and Synurus deltoides were cut off above ground part and the tip of the cutting were placed in water, which was covered with a plastic bag. Samples were kept overnight (about 12 hours) in darkness at room temperature (20~25$^{\circ}C$) to achieve maximal turgor (full saturation). The pressure in the chamber was gradually increased from 0.3MPa to 1.8MPa by nitrogen gas. After measured, leaf samples were dried at 80$^{\circ}C$ for 48 hours and dry weight of each samples were determined. The result of the original bulk osmotic potential at maximum turgor ${\Psi}^{sat}_o$ sat was lower -0.8 MPa in Aster scaber leaves than -0.7 MPa Synurus deltoides leaves. Also the osmotic potential at incipient plasmolysis ${\Psi}^{tlp}_o$ in Aster scaber leave was -0.9 MPa. In contrast, the value of maximum bulk modulus of elasticity $E_{max}$ of Aster scaber leaves were approximately two folds higher than that of Synurus deltoides leaves. The values of the relative water content at incipient plasmolysis $RWC^{tlp}$ are all above 90% showing that the function of osmoregulation is somewhat better, and Vo/DW, Vt/DW, Ns/DW of Synurus deltoides leaves were approximately 1~2 times higher than that of Aster scaber leaves. Thus, responses to water relations of Aster scaber and Synurus deltoides such as ${\Psi}^{sat}_o$, ${\Psi}^{tlp}_o$, $E_{max}$, ${\Psi}_{P,max}$, $RWC^{tl}$ were shown that the Aster scaber leaves was slightly higher drought-tolerance than Synurus deltoides leaves. However, in both of Aster scaber and Synurus deltoides, occurring incipient plasmolysis at the high water content, have a relatively lower drought-tolerance property indicating that growth of these plants are cultivated appropriate in high moisture soil sites.

COLOR DIFFERENCES BETWEEN RESIN COMPOSITES BEFORE- AND AFTER-POLYMERIZATION, AND SHADE GUIDES (복합레진의 광중합 전·후와 shade guide의 색차 비교)

  • Chon, Yi-Ju;Cho, Sung-Shik;Um, Chung-Moon
    • Restorative Dentistry and Endodontics
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    • v.24 no.2
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    • pp.299-309
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    • 1999
  • The composite resin, due to its esthetic qualities, is considered the material of choice for restoration of anterior teeth. With respect to shade control, the direct-placement resin composites offer some distinct advantages over indirect restorative procedures. Visible-light-cured (VLC) composites allow dentists to match existing tooth shades or to create new shades and to evaluate them immediately at the time of restoration placement. Optimal intraoral color control can be achieved if optical changes occurring during application are minimized. An ideal VLC composite, then, would be one which is optically stable throughout the polymerization process. The shade guides of the resin composites are generally made of plastic, rather than the actual composite material, and do not accurately depict the true shade, translucency, or opacity of the resin composite after polymerization. So the numerous problems associated with these shade guides lead to varied and sometimes unpredictable results. The aim of this study was to assess the color changes of current resin composite restorative materials which occur as a result of the polymerization process and to compare the color differences between the shade guides provided with the products and the actual resin composites before- and after-polymerization. The results obtained from this investigation should provide the clinician with information which may aid in improved color match of esthetic restoration. Five light activated, resin-based materials (${\AE}$litefil, Amelogen Universal, Spectrum TPH VeridonFil-Photo, and Z100) and shade guides were used in this study. Three specimens of each material and shade combination were made. Each material was condensed inside a 1.5mm thick metal mold with 10mm diameter and pressed between glass plates. Each material was measured immediately before polymerization, and polymerized with Curing Light XL 3000 (3M Dental products, USA) visible light-activation unit for 60 seconds at each side. The specimens were then polished sequentially on wet sandpaper. Shade guides were ground with polishing stones and rubber points (Shofu) to a thickness of approximately 1.5mm. Color characteristics were performed with a spectrophotometer (CM-3500d, Minolta Co., LTD). A computer-controlled spectrophotometer was used to determine CIELAB coordinates ($L^*$, $a^*$ and $b^*$) of each specimen and shade guide. The CIELAB measurements made it possible to evaluate the amount of the color difference values (${\Delta}E{^*}ab$) of resin composites before the polymerization process and shade guides using the post-polishing color of the composite as a control, CIE standard D65 was used as the light source. The results were as follows. 1. Each of the resin composites evaluated showed significant color changes during light-curing process. All the resin composites evaluated except all the tested shades of 2100 showed unacceptable level of color changes (${\Delta}E{^*}ab$ greater than 3.3) between pre-polymerization and post-polishing state. 2. Color differences between most of the resin composites tested and their corresponding shade guides were acceptable but those between C2 shade of ${\AE}$litefil and IE shade of Amelogen Universal and their respective shade guides exceeded what is acceptable. 3. Comparison of the mean ${\Delta}E{^*}ab$ values of materials revealed that Z100 showed the least overall color change between pre-polymerization and post-polishing state followed by ${\AE}$litefil, VeridonFil-Photo, Spectrum TPH, and Amelogen Universal in the order of increasing change and Amelogen Universal. Spectrum TPH, 2100, VeridonFil-Photo and ${\AE}$litefil for the color differences between actual resin and shade guide. 4. In the clinical environment, the shade guide is the better choice than the shade of the actual resin before polymerization when matching colors. But, it is recommended that custom shade guides be made from resin material itself for better color matching.

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Soil Characteristics according to the Geological Condition of Soil Slopes in Landslide Area (산사태지역 토층사면의 지질조건별 토질특성)

  • Kim, Kyeong-Su
    • The Journal of Engineering Geology
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    • v.16 no.4 s.50
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    • pp.359-371
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    • 2006
  • In this study, the soil characteristics are analyzed using the result of various soil tests as an object of the soil layer of natural slopes in landslides areas composed with gneiss, granite, and the tertiary sedimentary rock. To investigate the soil characteristics according to landslide and non landslide areas, soils are sampled from Jangheung, Sangju and Pohang. The landslides at three areas are occurred due to heavy rainfall in same time. The geology of Jangheung area, Sangju area and Pohang area is gneiss, granite, and the tertiary sedimentary rock, respectively. On the basis of the landslide data and the result of soil test, the soil characteristics at the landslide area and the differentiation between landslide area and non landslide area are analyzed. However soil characteristics have a little differentiation to geological condition, the uniformity coefficient and the coefficient of gradation of soils at the landslide area is larger than those of soils at the non landslide area. Also, the proportion of fine particle of soils at the landslide area is higher. The plastic limit of soils sampled from the granite and the sedimentary rock regions is larger than that sampled from the gneiss region. However the liquid limit is irrelevant to the geological condition. Also, the consistency of soils at the landslide area is smaller. The natural moisture content of soils in the sedimentary rock regions is larger than that of the granite and gneiss. It is mainly influenced by mineral composition, soil layer structure, weathering condition, and so on. The soils sampled from landslide area have higher porosity and lower density than those from non landslide area. It means that the soils of landslide area have poor particle size distribution and loose density. Therefore, the terrain slope with poor distribution and loose density is vulnerable to occur in landslides. Also, landslides are occurred in the terrain slope with high permeability. The permeability is mainly influenced by the soil characteristics such as particle size distribution, porosity, particle structure, and the geological origins such as weathering, sedimentary environment. Meanwhile, the shear strength of soils is little difference according to the geological condition. But, the internal friction angle of soils sampled from the landslide area is lower than that of soils from the non landslide area. Therefore, the terrain slope with low internal friction angle is more vulnerable to the landslide.

The Effects of Environment-Friendly Diets on the Growth Performance, Nutrient Digestibility, Fecal Excretion, Nitrogen Excretion and Emission Gases in Manure for Growing Pigs (환경친화적인 사료의 급여가 육성돈의 성장 능력, 영양소 소화율, 분 배설량, 분뇨내 질소배설량 및 악취 가스에 미치는 영향)

  • Yoo, J.S.;Cho, J.H.;Chen, Y.G.;Kim, H.J.;Wang, Q.;Hyun, Y.;Ko, T.G.;Park, C.S.;Kim, I.H.
    • Journal of Animal Science and Technology
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    • v.49 no.4
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    • pp.491-500
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    • 2007
  • Two experiments were used to determine the effects of environment-friendly diets on growth performance, fecal excretion, nitrogen excretion and emission gases in manure for growing pigs. In experiment 1, ninety six crossed pigs(Landrace×Yorkshire×Duroc) were allocated into four treatments. Treatments were AME(adequate ME diet, 3,265 kcal/kg), LME(lower ME diet, 3,100 kcal/kg), LME 0.05(lower ME diet+α- galactosidase & β-mannanase 0.05%) and LME 0.10(lower ME diet+α-galactosidase & β-mannanase 0.10%). Pigs fed AME diet had lower ADFI(Average Daily Feed Intake) than pigs fed other diets(p<0.05). DM(Dry Matter) digestibility in pigs fed AME and LME 0.10 diets had greater than pigs fed LME diet(p<0.05). Energy digestibility is higher in pigs fed AME and LME 0.10 diets than other treatments(p<0.05). In experiment 2, twenty four crossbred pigs(33.71 kg average BW) were used in a 14-d metabolism experiment. The pigs were housed in individual cages equipped with plastic bed flooring. Treatments were CP(Crude protein) 18% without Bacillus sp., CP 18% diet+Bacillus sp. 0.05%, CP 14% without Bacillus sp. and CP 14% diet+Bacillus sp. 0.05%. Nitrogen intake was higher for CP 18% diets than CP 14% diets(p<0.05). DM, N(Nitrogen) and energy digestibility were affected by probiotics(p<0.05). With the high CP in diets, Energy and N digestibility, urine N percent, urine N excretion and total N excretion were increased significantly compared to low CP in diets(p<0.05). Among the treatments, DM and N digestibilities, feces N excretion, N absorption were decreased significantly(p<0.05), however, feces excretion, feces N, urine N percent, urine N excretion and total N excretion were increased significantly(p<0.05) when pigs fed without probiotics diets compare to pigs fed with probiotics diets. DM and N digestibility, feces excretion, feces N excretion, urine N percent, urine N excretion, total N excretion, N absorption and N adsorption ratio were CP×probiotic interactions in p<0.05. Ammonia(p<0.01) and H2S(p<0.05) in manure were lower in CP 14% diets than CP 18% diets. Also, ammonia and H2S in manure were CP×probiotic interactions in p<0.05. In conclusion, low energy and reduction of CP dietary added enzyme and probiotics improved nutrient digestibility and reduced odors emission in manure for growing pigs.

Effect of Pollination Method on Fruit Setting and Quality of Oriental Melon(Cucumis melo L. var, makuwa Makino) (착과방법이 참외의 착과 및 품질에 미치는 영향)

  • Shin Yong Seub;Park So Deuk;Kim Jwoo Hwan;Kim Byung Soo
    • Journal of Bio-Environment Control
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    • v.14 no.2
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    • pp.83-88
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    • 2005
  • A study was conducted on three pollination methods on oriental melon(sageageol-ggul) grafting with pumpkin(seongjutozoa) for the labor-saving and to improve fruit set. Fruit weight, flesh thickness and fruit setting rate of oriental melon were greater in growth regulators treatment than those of pollinated by bees. Sugar content and hardness of fruits pollinated by bees were higher than those of by growth regulators. From the last ten days of the February to the first ten days of the March, fruit setting rate was $95\%$ in fruit setting growth regulators, whereas it was $46\%$ and $45\%$ in pollinated by honey and bumble bee, respectively. After the middle of March, the percentage of fruit setting was >$98\%$ in all the pollination methods. The cultivation under plastic houses of oriental melon, suitable fruiting time far the pollination by bees was decided after middle days of the March. Chromaticity and especially the value of 'a' of fruit of oriental melon pollinated by bees were higher than those of growth regulators. The percentage of fermented fruits of bee pollinated and growth regulators treated was $6.7\~9.1\%\;and\;28.1\%$, respectively. The weight of 100 seeds of bees pollinated were higher than that of growth regulators. The more increased the weight of 100 seeds the less appeared the rate of fermented fruits. The percentage of marketable fruits of the honey and bumble bee pollinated and that of growth regulators treated was $82\%,\;80.3\%\;and\;62.5\%$, respectively. The decreasing rate of fruit weight during storage of bees pollinated was less than those of growth regulators. In these results, the introduction of honey bee and bumble bee for the pollination of oriental melon was able to labor-saving of fruit set and increase of fruit quality.

Cyclic Seismic Testing of Cruciform Concrete-Filled U-Shape Steel Beam-to-H Column Composite Connections (콘크리트채움 U형합성보-H형강기둥 십자형 합성접합부의 내진성능)

  • Park, Chang-Hee;Lee, Cheol-Ho;Park, Hong-Gun;Hwang, Hyeon-Jong;Lee, Chang-Nam;Kim, Hyoung-Seop;Kim, Sung-Bae
    • Journal of Korean Society of Steel Construction
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    • v.23 no.4
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    • pp.503-514
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    • 2011
  • In this research, the seismic connection details for two concrete-filled U-shape steel beam-to-H columns were proposed and cyclically tested under a full-scale cruciform configuration. The key connecting components included the U-shape steel section (450 and 550 mm deep for specimens A and B, respectively), a concrete floor slab with a ribbed deck (165 mm deep for both specimens), welded couplers and rebars for negative moment transfer, and shear studs for full composite action and strengthening plates. Considering the unique constructional nature of the proposed connection, the critical limit states, such as the weld fracture, anchorage failure of the welded coupler, local buckling, concrete crushing, and rebar buckling, were carefully addressed in the specimen design. The test results showed that the connection details and design methods proposed in this study can well control the critical limit states mentioned above. Especially, the proposed connection according to the strengthening strategy successfully pushed the plastic hinge to the tip of the strengthened zone, as intended in the design, and was very effective in protecting the more vulnerable beam-to-column welded joint. The maximum story drift capacities of 6.0 and 6.8% radians were achieved in specimens A and B, respectively, thus far exceeding the minimumlimit of 4% radians required of special moment frames. Low-cycle fatigue fracture across the beam bottom flange at a 6% drift level was the final failure mode of specimen A. Specimen B failed through the fracture of the top splice plate of the bolted splice at a very high drift ratio of 8.0% radian.

Laterally Unbraced Length for Preventing Inelastic Lateral-Torsional Buckling of High-Strength Steel Beams (고강도 강재보의 비탄성 횡-비틀림좌굴 제어를 위한 횡지지 거리)

  • Park, Chang Hee;Lee, Cheol Ho;Han, Kyu Hong;Kim, Jin Ho;Lee, Seung Eun;Ha, Tae Hyu;Kim, Jin Won
    • Journal of Korean Society of Steel Construction
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    • v.25 no.2
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    • pp.115-130
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    • 2013
  • In this study, lateral-torsional buckling (LTB) strength of high-strength H-beams built up from 800MPa tensile-strength steel was experimentally and analytically evaluated according to current lateral stability provisions (KBC 2009, AISC-LRFD 2010). The motivation was to evaluate whether or not current LTB provisions, which were originally developed for ordinary steel with different stress-strain characteristics, are still applicable to high-strength steel. Two sets of compact-section specimens with relatively low (Set A) or high (Set B) warping stiffness were prepared and tested under uniform moment loading. Laterally unbraced lengths of the test specimens were controlled such that inelastic LTB could be induced. All specimens exhibited LTB strength exceeding the minimum limit required by current provisions by a sufficient margin. Moreover, some specimen in Set A reached a rotation capacity required for plastic design, although its laterally unbraced length belonged to the inelastic LTB range. All the test results indicated that extrapolation of current provisions to high-strength steel is conservative. In order to further analyze the test results, the relationship between inelastic moment and laterally unbraced length was also derived in explicit form for both ordinary- and high-strength steel based on the effective tangent modulus of inelastic section. The analytical relationship derived again showed that extrapolation of current laterally unbraced length limit leads to a conservative design in the case of high-strength steel and that the laterally unbraced length to control the inelastic LTB behavior of high-strength steel beam should be specified by including its unique post-yield strain-hardening characteristics.

Effect of COY (Cooking Oil and Yolk mixture) and ACF (Air-circulation Fan) on Control of Powdery Mildew and Production of Organic Lettuce (난황유와 공기순환팬의 상추 흰가루병 방제효과 및 생산에 미치는 영향)

  • Jee, Hyeong-Jin;Ryu, Kyung-Yul;Park, Jong-Ho;Choi, Du-Hoe;Ryu, Gab-Hee;Ryu, Jae-Gee;Shen, Shun-Shan
    • Research in Plant Disease
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    • v.14 no.1
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    • pp.51-56
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    • 2008
  • Powdery mildew of lettuce that is a newly reported disease became a threat to organic cultivation of lettuce in Korea since the disease caused by Podosphaera fusca resulted in a half of yield loss in heavily infected fields. To improve micro-environmental conditions around lettuce, ACF (air-circulation fan) was installed on inside roof of plastic house at 6 m intervals. The ACF increased 57% of lettuce yield and reduced 71.4% of lettuce seedling death. COY (cooking oil and yolk mixture) consisted of cooking oil 0.3% and egg yolk 0.08% reduced lettuce seedling death from 89.3% to 92.9% under the greenhouse. Seven-day interval spray of COY resulted in high control values of powdery mildew of lettuce ranging from 89.6% to 96.3%, which was comparable to a fungicide, Azoxystrobin. Lettuce yield was increased about two times compared to a non-treated conventional cultivation. Qualities of lettuce such as hardness and chlorophyll content were also improved by COY and ACF combination. Effect of COY on control of the disease was improved when $CaCO_3$ or $SiO_2$ 1,000 ppm was supplemented. Results indicated that the COY made of cooking oil such as canola emulsified with yolk was highly effective on control of powdery mildew of lettuce and suitable for organic agriculture, especially when combined with ACF.

Effect of Nitrogen fertilizers on Soil pH, EC, NO3-N and Lettuce(Lactuca Sativa. L.) Growth (질소비종이 토양의 pH, EC, NO3-N 함량 및 상추 생육에 미치는 영향)

  • Lee, Gyeong-Ja;Kang, Bo-Koo;Kim, Hyun-Ju;Park, Seong-Kyu;Min, Kyeong-Beom
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.2
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    • pp.122-128
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    • 2001
  • In order to find out the effect of nitrogen fertilizers on soil pH, EC, $NO_3-N$ and lettuce growth, this study was conducted by pot experiment in plastic film house condition. The square-pot which was $42{\times}54.5{\times}22cm$ in length, width and height, respectively, was filled with two kinds of soils in different soil EC as $0.20dS\;m^{-1}$ and $1.13dS\;m^{-1}$. Seven kinds of nitrogen fertilizers (urea, potassium nitrate, calcium nitrate, ammonium nitrate, ammonium sulphate, complex fertilizer A(11-10-10) and complex fertilizer B(12-12-12)) were treated in same standard rate of nitrogen for lettuce, transplanted the six lettuce seedlings of 10 days grown per pot, and have been grown for 38~44 days with three times harvesting. Soil pH was increased with the potassium and calcium nitrate treatments and decreased with ammonium nitrate, ammonium sulphate, complex fertilzer A and B, and the pH of urea treatments was kept the same value as the pH of before experiment. The growing status of lettuce seedling were surveyed during the early period after transplanting and withering of seedling was occured in all treatments. The withering rates were 10% in soil of EC $0.20dS\;m^{-1}$ and 44% and 42% in complex fertilizer and ammonium sulphate treaments, respectively, in soil of EC $1.13dS\;m^{-1}$. $NO{_3}^-$ contents of lettuce were about $1,000{\sim}2,000mg\;kg^{-1}$ based on fresh weight and these contents were considered to be lower to compare the $NO{_3}^-$ level of EU countries.

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Surface maker and gene expression of human adipose stromal cells growing under human serum. (인체혈청 하에서 배양한 인체지방기질줄기세포의 표면항원 및 유전자 발현)

  • Jun, Eun-Sook;Cho, Hyun-Hwa;Joo, Hye-Joon;Kim, Hoe-Kyu;Bae, Yong-Chan;Jung, Jin-Sup
    • Journal of Life Science
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    • v.17 no.5 s.85
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    • pp.678-686
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    • 2007
  • Human mesenchymal stem cells(hMSC), that have been reported to be present in bone marrow, adipose tissues, dermis, muscles and peripheral blood, have the potential to differentiate along different lineages including those forming bone, cartilage, fat, muscle and neuron. Therefore, hMSC are attractive candidates for cell and gene therapy. The optimal conditions for hMSC expansion require medium supplemented with fetal bovine serum(FBS). Some forms of cell therapy will involve multiple doses, raising a concern over immunological reactions caused by medium-derived FBS proteins. Previously, we have shown that hADSC can be cultured in human serum(HS) during their isolation and expansion, and that they maintain their proliferative capacity and ability for multilineage differentiation and promote engraftment of peripheral blood-derived CD34 cells mobilized from bone marrow in NOD/SCID mice. In this study we determined whether hADSC grown in HS maintain surface markers expression similar with cells grown in FBS during culture expansion and compared gene expression profile by Affymetrix microarray. Flow cytometry analysis showed that HLA-DR, CD117, CD29 and CD44 expression in HS-cultured hADSC during culture expansion were similar with that in FBS-cultured cells. However, the gene expression profile in HS-cultured hADSC was significantly different from that in FBS-cultured cells. Therefore, these data indicated that HS-cultured hADSC should be used in vivo animal study of hADSC transplantation for direct extrapolation of preclinical data into clinical application.