• 분석과학
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• 제25권5호
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• pp.265-272
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• 2012

기존의 Okamoto cavity를 변형시킨 WR-340 도파관을 사용한 cavity를 제작하고 고출력(2.45 GHz, 2 kW)의 헬륨, 질소 및 아르곤 마이크로파 플라즈마(MIP; Microwave Induced Plasma)를 성공적으로 형성시켰다. 플라즈마 생성의 주요한 요인들은 내부전도체의 직경과 내부전도체와 외부전도체간의 간격, 내부전도체 끝과 토치의 위치 등이 있으며 그 중 헬륨 마이크로파 플라즈마에 대하여 cavity의 디자인을 최적화시키고 그 특성을 조사하였다. ICP(Inductively Coupled Plasma)용 mini 토치와 자체 제작한 나선형흐름토치를 비교 연구한 결과, 헬륨 플라즈마 기체 흐름량은 약 25 L/min~30 L/min로서 서로 비슷하였다. 토치 상단부에 석영관을 덧씌워 공기유입을 막은 결과, 340 nm 근처의 NH분자선들이 없어지거나 감소하였다. 플라즈마의 온도 및 전자밀도를 측정한 결과, 4,350 K의 들뜸 온도와 $3.67{\times}10^{11}/cm^3$의 전자밀도를 얻었다. 이 값들은 기존의 다른 마이크로파 플라즈마와 비슷하거나 약간 작은 값이다. 고출력의 플라즈마로서 수용액을 직접 분석하는 것이 가능하였고 현재 Cl의 검출한계는 116 mg/L 수준으로서 아직 분석적인 최적화가 필요한 단계이다.

• 대한한방내과학회지
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• 제22권4호
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• pp.703-707
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• 2001

Each decoction of Coptidis Rhizoma, Scutellariae Radix and Phellodendri Cortex was orally administrated to the rates. And the effects on the Gastrin and Uropepsin secretions were observed. The results were as follows: 1. The effects on the secretio of Gastrin in the experimental groups administrered each with Coptidis Rhizoma and Scutellariae Radix comparing with the normal group, showed signficant values P<0.05, P<0.02, P<0.01 and P<0.001 separately. However the group medicated with Phellodendri Cortex showed significant values only 30 minutes after the administration. 2. Uropepsion level in the experimental group administered each with Coptidis Rhizoma and Phellodendri Cortex, comparing with the normal group, showed highly significant statistic values (P<0.001). Another experimental group medicated with Scutellariae Radix showed significantly statistical values (P<0.001) 240 minutes after the administration. 3. According to the above mentioned results it was onculuded tat the secretion of Gastrin in plasma and Uropepsin content of urine in albino rats treated with Coptiodis Rhizoma decoction were remarkaby increased as comparing with Scutellariae Radix and Phellodendri Cortex decoction.

• Environmental Analysis Health and Toxicology
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• 제25권1호
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• pp.27-40
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• 2010

The current study was designed to scientifically evaluate the atmospheric particulate pollution in residences relative to their proximity to a Korean major iron/metal industrial complex (IMIC). This objective was achieved by measuring the concentrations and elemental composition of particulate matter with aerodynamic diameters equal to or less than 10 ${\mu}m$(PM10) in industrial ambient air from IMIC and residential ambient air with relative proximities to IMIC. The trace metals were analyzed using an inductively coupled plasma-atomic emission spectroscopy (ICP-AES). The industrial mean values exceeded the Korean year/70-${\mu}g/m^3$ standard for PM10, whereas the residential mean values did not. However, the maximum residential values did exceed or were close to the Korean PM10 year standard. For individual elements, the ambient concentrations ranged widely from values in the order of a few $ng/m^3$ to thousands of $ng/m^3$. The residential mean mass concentrations in the PM10 measured in the present study were higher than or similar to those reported in earlier studies. This study suggests that residents in neighborhoods near the IMIC are exposedto elevated particulate levels compared to residents living further away from such a source.

• 멤브레인
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• 제20권3호
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• pp.197-202
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• 2010

당뇨병 환자의 혈당치 측정을 위하여 폴리우레탄으로 만들어진 진단막을 제조하였다. 혈액 속의 글루코우즈의 농도를 변화시켜가며 폴리우레탄 진단막을 가지고 680 nm에서의 최종흡광도를 측정하였다. 시간에 따른 흡광도 변화량(K/S)의 최종 결과치가 글루코우즈의 농도가 증가함에 따라 직선적으로 증가하였다. 헤마토크릿이 글루코우즈의 농도 측정에 미치는 영향을 조사하였다. 낮은 헤마토크릿에서는 글루코우즈의 농도와 K/S와의 기울기 값(Dose-Response Slope : DRS)이 플라즈마와 비교해 큰 차이를 보이지 않았다. 그러나 높은 헤마토크릿(40% 이상)에서는 상당히 감소함을 알 수 있었다.

• Nuclear Engineering and Technology
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• 제54권2호
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• pp.423-428
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• 2022

Very low contents (in the range of 10^-9 g/g) of Ir in mantle-derived rock samples (komatiites) were non-destructively determined by INAA coupled with coincidence gamma-ray spectrometry using 16 Ge detectors. Aliquots of the same samples were analyzed by NiS fire-assay ICP-MS for Ir and other platinum group elements. Because the INAA procedure used in this study is non-destructive and is almost free from spectral interference in gamma-ray spectrometry, the INAA values of Ir contents obtained in this study can be highly reliable. Iridium values obtained by ICP-MS were consistent with the INAA values, implying that the ICP-MS values of Ir obtained in this study are equally reliable. Under the present experimental conditions, detection limits were estimated to be 1 pg/g, which corresponds to 0.1 pg for a sample mass of 0.1 g. These levels can be even lowered by an order of magnitude, if necessary, which cannot be achieved by ICP-MS carried out in this study.

• 대한임상검사과학회지
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• 제50권2호
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• pp.197-204
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• 2018

다발성 골수종(MM)은 혈액 종양의 주요 사망 원인이다. 최근에 다발성 골수종 진단에 microRNA를 이용한 실험이 보고되고 있다. 이에 우리는 다발성 골수종 진단 마커로 miR-221을 이용 할 수 있는지 확인하고자 하였다. 본 연구는 다른 혈액학적 질환이 없는 다발성 골수종 환자 20명을 대상으로 하였다. MicroRNA 추출은 다발성 골수종 환자의 파라핀 포매 조직을 이용하였다. 우리는 다발성 골수종의 microRNA 표적 유전자로 miR-15a, miR-16, miR-21, miR-181a 그리고 miR-221을 선택하였다. 유의성 검정은 fold change 값을 기준으로 1.5이상 또는 -1.5 미만의 결과를 유의성이 있는 경우로 하였다. Fold change 값은 인간 유전자 SNORD43에 의해 표준화된 데이터를 기준으로 하였다. Fold change 값이 1.5 이상은 "overexpression", -1.5 미만의 값은 "underexpression"으로 정의되었다. miR-221의 65.0% (13/20)에서 "overexpression"으로 유의성이 있음을 확인하였고, 다발성 골수종 환자에서 형질세포가 30% 이상인 그룹과 이하의 그룹은 유의성을 보이지 않았다. MiR-221은 서구인과 같은 결과를 얻었으며, 다발성 골수종 환자에서 miR-221이 다발성 골수종 환자 진단에 매우 중요한 지표가 될 수 있을 것으로 생각된다. 결론적으로 miR-221은 한국인의 다발성 골수종 진단 또는 예후 지표로 활용할 수 있음을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권8호
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• pp.1201-1207
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• 2007

This study compared the recovery rate of intrajugular-administered allantoin in the urine and saliva between swamp buffaloes and zebu cattle to examine whether it could explain the lower excretion rate of urinary purine derivatives (PD) in the buffaloes. Three male swamp buffalo yearlings, with an average body weight of $349{\pm}40.35$ kg, and three Thai native cattle ($154{\pm}3.26$ kg) of similar age and sex were used in the study. Animals were kept in individual pens and fed at a maintenance energy level with a diet containing 65% monk bean husk (Vigna radiata) as roughage and 35% concentrates. Allantoin solution was infused into the jugular vein in four incremental rates equivalent to 0, 5, 10 and 15 mmol/d and urine was collected daily in acidified form. Daily PD excretion was linearly correlated with intrajugular allantoin infusion in both species. The relationship between daily urinary PD excretion (Y, mmol/d) and intrajugular allantoin infused (X, mmol/d) was $Y=0.75{\pm}0.318X+22.45{\pm}2.98$ ($r^2$ = 0.36, n = 12, MSE = 38.02, CV = 21.9, p<0.01) for swamp buffaloes and $Y=0.96{\pm}0.10X+15.93{\pm}0.92$ ($r^2$ = 0.91, n = 12, MSE = 3.60, CV = 8.27, p<0.01) for zebu cattle. The salivary PD concentration was not correlated with intrajugular allantoin infusion in both species, with values for buffaloes numerically lower than those for cattle. The present study reconfirmed previous studies that buffaloes have a lower plasma PD excretion rate via the renal route and a significant proportion (22%) of the plasma PD loss is via the saliva. However, results of our present and previous studies suggest that differences in purine base (PB) metabolism between buffaloes and zebu cattle occur before the purine compounds reach the plasma pool.

• 한국임상약학회지
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• 제18권2호
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• pp.106-113
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• 2008

The aim of this study was to evaluate the bioequivalence of two domperidone preparations. Bioequivalence assessment was conducted on 34 healthy volunteers who received two tablets (Domperidone Maleate, 12.72 mg/tablet) in the fasting state, in a randomized balanced $2{\times}2$ cross-over study design. This whole study was performed according to the implementation guidelines of the Korea Food Drug Administration. After dosing of two tablets, blood samples were collected serially for a period of 36 hours. Plasma was analyzed for domperidone by using LC/MS/MS assay method. The analysis system was validated in specificity, accuracy, precision, and linearity. $AUC_t$, (the area under the plasma concentration-time curve from the zero-time to 36 hr) was calculated through the trapezoidal rule. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma domperidone concentration-time data of each volunteer. No significant sequence effect was found for the bioavailability parameters indicating that the cross-over design was properly performed. The 90%-Confidence intervals of the $AUC_t$ ratio and the $C_{max}$ were from log 0.8007 to log 1.1240 and log 0.8645- log 1.2483, respectively. These values were within the acceptable bioequivalence intervals between 0.80 and 1.25. Therefore, this study demonstrated that two formulations have bioequivalence with respect to the rate and extent of absorption.

• Journal of Pharmaceutical Investigation
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• 제38권4호
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• pp.277-282
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• 2008

The purpose of the present study was to evaluate the bioequivalence of two topiramate tablets, Topamax tablet (Janssen Korea. Co., Ltd., Seoul, Korea, reference drug) and Topamin tablet (Myungmoon Pharm. Co., Ltd., Seoul, Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). Twenty-four healthy male Korean volunteers received one tablet at the dose of 100 mg topiramate in a $2{\times}2$ crossover study. There were two-weeks washout period between the doses. Plasma concentrations of topiramate were monitored by an LC-MS/MS for over a period of 96 hr after administration. $AUC_t$ (the area under the plasma concentration-time curve from time zero to 96 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum plasma drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the plasma concentration-time data. Analysis of variance (ANOVA) was carried out using logarithmically transformed $AUC_t$ and $C_{max}$. The 90% confidence intervals of the $AUC_t$, ratio and the $C_{max}$ ratio for Topamin/Topamax were $\log0.88{\sim}\log1.02$ and $\log0.87{\sim}\log1.03$, respectively. These values were within the acceptable bioequivalence intervals of $\log0.80{\sim}\log1.25$. Taken together, our study demonstrated the bioequivalence of Topamax and Topamin with respect to the rate and extent of absorption.

• 한국전기전자재료학회논문지
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• 제28권5호
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• pp.300-305
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• 2015

$WO_3$, $SiO_2$, and $TiO_2$ films with hydrophilic property are deposited by rf-magnetron sputtering. Their wettability is strongly depends on the presence or absence of the oxygen plasma etching on the glass substrates. The $TiO_2$ film of 50 nm-thick on the plasma etched glass shows a water contact angle (WCA) below $5^{\circ}$ which means a super-hydrophilic surface. However, WCA values are gradually degraded when the films are exposed under atmosphere, especially $WO_3$. In order to improve hydrophilic property, the degraded films can be again recovered by UV illumination for 10 sec using UV-light and the $TiO_2$ film shows a super-hydrophilic surface about $3^{\circ}$.