• Title/Summary/Keyword: plasma activated

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Formation of the functional plasma ploymerized thin films by a new type reactor (새로운 반응장치를 이용한 기능성 플라즈마 중합막의 제작)

  • 김종택;이상희;박종관;박구범;이덕출
    • Journal of the Korean Vacuum Society
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    • v.7 no.1
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    • pp.72-76
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    • 1998
  • A new gas-flow type reactor for plasma polymerization was developed to synthesize functional polymers, which enhances reaction of radicals activated in the discharge. Styrene was used for the plasma polymerization and molecular strucure and molecular weight distribution of the plasma -polymerized styrene were studies. The ploymer was evaluated to be an efficient electron beam resist. The sensitivity of the plasma-polymerized styrene film formed by this new reactor was better than that of the reported values of conventional polystyrene, Fine resist patterns could be successfully developed by a wet process.

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FABRICATION OF PLATELET-RICH PLASMA IN A RAT MODEL AND THE EFFICACY TEST IN VITRO (백서에서 혈소판 풍부 혈장의 제작과 유효성에 대한 실험적 연구)

  • Lee, Sang-Hoon;Cho, Young-Uk;Chi, Hyun-Sook;Ahn, Kang-Min;Lee, Bu-Kyu
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.29 no.2
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    • pp.113-122
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    • 2007
  • Purpose: Platelet-rich plasma (PRP) is known to accelerate and/or enhance hard and soft tissue healing and regeneration. As such, PRP has been used in various clinical fields of surgery. Recently there have been several attempts to use PRP in the field of tissue engineering. However, some controversies still exist on exact mechanism and benefits of PRP. Therefore various animal experiments are necessary to reveal the effect of the PRP. However, even if animal experiment is performed, the efficacy of the experiment could not be validated due to absence of an animal PRP model. The purpose of this study is to establish rat PRP model by comparing several PRP fabricating methods, and to assay growth factor concentration in the PRP. Materials and methods: Rat blood samples were collected from nine SD rat (body weight: 600-800g). PRP was prepared using three different PRP fabricating methods according to previously reported literatures. (Method 1: 800 rpm, 15 minute, single centrifuge; Method 2: 1000 rpm, 10 minute, double centrifuge; Method 3: 3000 rpm, 4min and 2500 rpm, 8 min, double centrifuge). Platelet counts were evaluated in an automated machine before and after PRP fabrications. In terms of growth factor assay, prepared PRP were activated with 100 unit thrombin and 10% calcium chloride. Growth factor (PDGF-BB, VEGF) concentrations on incubation time were determined by sandwich-ELISA technique. Results: An average of 3ml (via infraorbital venous plexus) to 15ml (via celiac axis) the rat blood could be collected. By using Method 3 (3000 rpm, 4 min and 2500 rpm, 8 min, double centrifugation), around 1.5ml of PRP could be prepared. This method allowed us to concentrate platelet 3.77-fold on average. PDGF-BB concentration (mean, 1942.10 pg/ml after 1 hour incubation) and VEGF concentration (mean, 952.71 pg/ml after 1 hour incubation) in activated PRP were higher than those in untreated blood. Also PDGF-BB showed constant concentration during 4-hour incubation, while VEGF concentration was decreased after 1 hour. Conclusion: Total 11,000 g minute separation and condensation double centrifuge method can produce efficient platelet-rich plasma. Platelet-rich plasma activated with thrombin has showed higher concentrations of growth factors such as PDGF-BB and VEGF, compared to the control group. Platelet-rich plasma model in a rat model was confirmed in this study.

XPS study of sapphire substrate surface nitridated by plasma activated nitrogen source (Plasma로 활성화된 질소 원자를 사용한 사파이어 기판 표면의 저온 질화처리의 XPS 연구)

  • 이지면;백종식;김경국;김동준;김효근;박성주
    • Journal of the Korean Vacuum Society
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    • v.7 no.4
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    • pp.320-327
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    • 1998
  • The chemical aspects of nitridated surface of sapphire(0001) have been studied by X-ray photoelectron spectroscopy. Nitridated layer was formed by remote plasma enhanced-ultrahigh vacuum deposition at a low temperature range. It was confirmed that this nitridated surface was mainly consists of AIN layer. The relative amounts of nitrogen reacted with AL on the sapphire surface and their surface morphology were investigated with X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) as a function of radio-frequency power, reaction temperature, and reaction time. The amounts of atomic nitrogen activated by plasma which was subsequently incorporated into sapphire were increased with RF power. But the amounts of nitrogen reacted with AI in sapphire was initially increased and then remained constant. However, the relative amounts of AIN were nearly constant with irrespective of nitridation temperature and time. Furthermore, a depth porfile of nitridated layer with XPS showed that the nitridated surface consisted of three layers with different stoichiometry.

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ATP Modulation of Cloned Rat Brain Large-conductance $Ca^{2+}$-activated $K^+$ Channel by Protein Phosphorylation

  • Park, S.Y.;S. Chung
    • Proceedings of the Korean Biophysical Society Conference
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    • 1999.06a
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    • pp.63-63
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    • 1999
  • Large conductance $Ca^{2+}$-activated $K^{+}$ channels (Maxi-K channel) have been implicated in many important physiological processes such as co-ordination of membrane excitability in neurons. Modulation of these channels are archived by the activity of various protein kinases. The most widely studied example of Maxi-K channel regulation by protein phosphorylation has been obtained using plasma membranes from the rat brain incorporated into lipid bilayers.(omitted)

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Refining of Invar and Permalloy Fe-Ni Alloys by $Ar/Ar-H_2$ Plasma and Electron Beam Melting (Ar/Ar-$H_2$ 플라즈마 및 전자선 용해에 의한 인바 및 퍼멀로이 Fe-Ni 합금의 정련)

  • Park, Byung-Sam;Baik, Hong-Koo
    • Journal of Korea Foundry Society
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    • v.15 no.2
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    • pp.175-183
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    • 1995
  • It is difficult to remove such interstitial impurities as sulfur, oxygen, hydrogen and carbon in Fe-Ni alloys. Thermodynamic and kinetic studies were carried out on the behavior of hydrogen gas, oxygen gas, Si, Al and slag, and the reaction time by the $Ar/Ar-H_2$ plasma and electron beam melting. After the addition of Al, Si, they were melted by Ar plasma with reaction time changed. 80%Ni-Fe alloys showed a better deoxidation than 36%Ni-Fe alloys. At $Ar-H_2$ plasma melting, the deoxidation was significant. In the case of the electron beam melting, the residual oxygen was higher than in Ar plasma melting because electron beam melting temperature was lower than that of Ar plasma. For the decaburization, it was melted by $Ar-O_2$ plasma melting, which could remove effectively carbon by activated oxygen in plasma. We added slag to Fe-Ni alloys for the desulfurization. As the result of this experiments, the amount of residual sulfur was not changed according to the slag ratio and reaction time.

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Film Properties of TiO2 Made by Activated Reactive Evaporation (활성화 반응으로 제작된 TiO2의 박막특성)

  • Park, Yong-Gwon;Choi, Jae-Ha
    • Journal of the Korean Society for Heat Treatment
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    • v.14 no.3
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    • pp.151-154
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    • 2001
  • $TiO_2$ thin film has wide application because of its high capacitanca, reflection, and good transmissivity in visible range. $TiO_2$ thin film can be made by thermal deposition method, reactive evaporation method, activated reactive evaporation(ARE) method. In the case of thermal deposition, the oxygen deficiency can occur because the melting point of Ti is very high. While in the case of reactive evaporation, high density $TiO_2$ can not be made, because reactive gas($O_2$) and evaporated material(Ti) are not fully combined, activated reactive evaporation, $TiO_2$ is easily deposited at lower gas pressure compared with reactive evaporation because the ionized reactive gas is made by plasma. Therefore, activated reactive evaporation is very useful to deposit the material having the high melting point. In this work, we formed $TiO_2$ thin film by activated reactive evaporation method. The surface of $TiO_2$ thin film was analyzed by X-ray photoelectron spectroscopy. The surface morphology which was analyzed by atomic force microscopy(AFM) shows that feature of the film surface is uniform. The dielectric capacitance, withstanding voltage were $600{\mu}F/cm^2$, 0.4V respectively. In further work, we can increase the withstanding voltage by improving the deposition parameter of substrates.

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A Study of Non-thermal Plasma Generation on a Photocatalytic Reactor Using a Ceramic Honeycomb Monolith Substrate (세라믹 벌집형 담체를 사용한 광촉매 반응기의 플라즈마 생성에 관한 연구)

  • 손건석;윤승원;고성혁;김대중;송재원;이귀영
    • Transactions of the Korean Society of Automotive Engineers
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    • v.10 no.2
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    • pp.48-54
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    • 2002
  • Since photocatalysts are activated by lights of UV wavelengths, plasma is alternatively used as a light source for a photocatalytic reactor. Light intensity generated by plasma is proportional to the surface area of catalytic material, and this, in many practical applications, is prescribed by the geometry of a plasma generator. Thus, it is crucial to increase the surface area far sufficient light intensity for photocatalytic reaction. For example, in a pack-bed type reactor, multitudes of beads are used as a substrate in order to increase the surface area. Honeycomb monolith type substrate, which has very good surface area to volume ratio, has been difficult to apply plasma as a light source due to the fact that light penetration depth through the honeycomb monolith was too short to cover sufficient area, thus resulting in poor intensity for photocatalytic reaction. In this study, nonthermal plasma generation through a photocatalytic reactor of honeycomb monolith substrate is investigated to lengthen this short penetration depth. The ceramic honeycomb monolith substrate used in this study has the same length as a three way catalyst used fur automotive applications, and it is shown that sufficient light intensity for photocatalytic reaction can also be obtained with honeycomb monolith type reactor.