• Korean Journal of Plant Tissue Culture
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• v.28 no.3
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• pp.147-151
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• 2001

Explants of Lactuce sativa cultivar, chungchima, were co-cultivated with Agrobacterium tumefaciences LBA4404, EHA101 strains containing nptll gene and ferritin gene encoding iron storage protein from soybean for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 MS basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were tested with PCR analysis using nptll, ferritin specific primers whether ferritin gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot showed that transcripts of ferritin gene were detected in mature leaf of the transgenic lines. These results suggest that ferritin gene be successfully integrated and transcribed in the putative transgenic lettuce plants.

• Molecules and Cells
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• v.26 no.1
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• pp.1-4
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• 2008

A gene's influence on an organism's Darwinian fitness ultimately determines whether it will be lost, maintained or modified by natural selection, yet biologists have few gene expression systems in which to measure whole-organism gene function. In the Department of Molecular Ecology at the Max Planck Institute for Chemical Ecology we are training "molecularly enabled field biologists" to use transformed plants silenced in the expression of environmentally regulated genes and the plant's native habitats as "laboratories." Research done in these natural laboratories will, we hope, increase our understanding of the function of genes at the level of the organism. Examples of the role of threonine deaminase and RNA-directed RNA polymerases illustrate the process.

• Korean Journal of Plant Resources
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• v.17 no.1
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• pp.20-23
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• 2004

These studies were carried out to develop native Calanthe in Korea. Calanthe native to southern islands in korea has beautiful flowers with various color and sweet fragrance, and it has been reported to have very good ornamental value. Concerning the classified 57 lines had surveyed their characteristics during the three years, and confirmed which that had manifestation stably. And then, a line was developed and given a name of horticultural cultivar to ‘Saegdong’. The major characteristics of the selected line, ‘Saegdong’, were as follows. In its color of flower, sepal was reddish orange, petal was yellow and lip was yellow. ‘Saegdong’ had a bended-inner blooming type.

• Korean Journal of Plant Resources
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• v.13 no.3
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• pp.155-161
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• 2000

Many Cordyceps rpp were collected for excellent strains selection at Mts. Moak, Younsuk and Daea-ree herbarium near Wanju-kun from mid May to October,1999. They were identified as the resulting 2 genera and 5 species, 10 strains were got through 5 species culture. Among them 4 species used for induction of artifitial fruiting body and 6 strains of them were selected to excellent strains: Cordyceps militaris (99-0007), Isaria japonica (99-0001, 99-0002, 99-0003), Cordyceps soborifera (99-0008), Cordyceps kanzashiana(99-0009).

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.14-14
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• 2017

The discipline of plant breeding is experiencing a renaissance impacting crop improvement as a result of new technologies, however fundamental questions remain for predicting the phenotype and how the environment and genetics shape it. Inexpensive DNA sequencing, genotyping, new statistical methods, high throughput phenotyping and gene-editing are revolutionizing breeding methods and strategies for improving both quantitative and qualitative traits. Genomic selection (GS) models use genome-wide markers to predict performance for both phenotyped and non-phenotyped individuals. Aerial and ground imaging systems generate data on correlated traits such as canopy temperature and normalized difference vegetative index that can be combined with genotypes in multivariate models to further increase prediction accuracy and reduce the cost of advanced trials with limited replication in time and space. Design of a GS training population is crucial to the accuracy of prediction models and can be affected by many factors including population structure and composition. Prediction models can incorporate performance over multiple environments and assess GxE effects to identify a highly predictive subset of environments. We have developed a methodology for analyzing unbalanced datasets using genome-wide marker effects to group environments and identify outlier environments. Environmental covariates can be identified using a crop model and used in a GS model to predict GxE in unobserved environments and to predict performance in climate change scenarios. These new tools and knowledge challenge the plant breeder to ask the right questions and choose the tools that are appropriate for their crop and target traits. Contemporary plant breeding requires teams of people with expertise in genetics, phenotyping and statistics to improve efficiency and increase prediction accuracy in terms of genotypes, experimental design and environment sampling.

• Nuclear Engineering and Technology
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• v.50 no.4
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• pp.599-605
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• 2018

Fault diagnosis depends critically on the selection of sensors monitoring crucial process variables. Boolean network (BN) is composed of nodes and directed edges, where the node state is quantized to the Boolean values of True or False and is determined by the logical functions of the network parameters and the states of other nodes with edges directed to this node. Since BN can describe the fault propagation in a sensor network, it can be applied to propose sensor selection strategy for fault diagnosis. In this article, a sufficient condition for parameter identifiability of BN is first proposed, based on which the sufficient condition for fault identifiability of a sensor network is given. Then, the fault identifiability condition induces a sensor selection strategy for sensor selection. Finally, the theoretical result is applied to the fault diagnosis-oriented sensor selection for a nuclear heating reactor plant, and both the numerical computation and simulation results verify the feasibility of the newly built BN-based sensor selection strategy.

• Korean Journal of Plant Tissue Culture
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• v.24 no.4
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• pp.225-231
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• 1997

For genetic engineering to be commercially viable, an efficient transformation system is needed to produce transgenic plane from diverse genotypes ("generalized protocol"). Development of such a system requires optimization of a number of components such as gene transfer agent, plant tissues competent for both regeneration and transformation, and control of transgene expression. Although several novel gene transfer methods have been developed for plane, a majority of stably transformed plane express the introduced genes at low levels. Moreover, silencing of selectable marker genes shortly after their incorporation into plant chromosomes may result in low recovery of transgenic tissues from selection. Matrix attachment regions (MARs) are DNA sequences that bind to the cell's proteinaceous nuclear matrix to form DNA loop domains. MARs have been shown to increase transgene expression in tobacco cells, and reduce position in mature transgenic plants. Flanking an antibiotic resistance transgene with MARs should therefore lead to improved rates of transformation in a diversity of species, and may permit recalcitrant species and genotypes to be successfully transformed. Literature review and recent data from my laboratory suggest that MARs can serve as a transformation booster in recalcitrant plant species.

• Plant Resources
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• v.3 no.3
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• pp.206-210
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• 2000

A total of 20 soybean recommended varieties which were developed until late 1980's in Korea was evaluated at Suwon. Comprehensive evaluation and correlation analysis were conducted on the agronomic characters. Great variations were found in these genotypes for branch number, pod number, and grain yield per plant. The variation in number of pods/plant ranged from 53 to 164, and in grain yield from 25.9 to 68.8 g. The coefficient of variation for most of the characters had a wide range. In correlation coefficient, grain yield per plant showed a positive phenotypic association with weight of pods, pod number of branches, and weight of stem. Multiple regression analysis was done to formulate selection criteria. It indicated that stout and medium-stature genotypes with more branches, resulting in varieties with more pods per plant but with medium-size seeds are available to obtain high-yielding varieties.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.145.1-145
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• 2003

This study was conducted to designing conserved regions of molecules for virus-derived resistance to transgenic Phlaenopsis orchids to protect against two major orchid viruses, Cymbidum mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). Infected leaf samples of Phalaenopsis were randomly screened by the RT-PCR with specific primers to both of viruses. RT-PCR products of the viruses were cloned and their nucleotide sequences were determined. Multiple alignments of coat protein (CP) genes of the viruses revealed that over the 88 ％ and 94 ％ identities with CymMV and ORSV, respectively, were observed. These data can be useful for selection of highly conserved regions of CP gene of the viruses for transgenic orchid experiments.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.89-103
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• 2011

Two rice varieties, viz. Nonabokra and Pokkali, have been evaluated for their responses to salinity in terms of some physiological and biochemical attributes. During the exposure to salinity (200 mM concentration of sodium chloride for 24, 48, and 72 h), a significant increase in sodium was recorded which was also concomitant with the changes of other metabolic profiles like proline, phenol, polyamine, etc. The protein oxidation was significantly increased and also varied between the two cultivars. The changes in activities of anti-oxidative enzymes under stress were significantly different to the control. The detrimental effects of salinity were also evident in terms of lipid peroxidation, chlorophyll content, protein profiles, and generation of free radicals; and these were more pronounced in Pokkali than in Nonabokra. The assessment and analysis of these physiological characters under salinity could unravel the mechanism of salt responses revealed in this present study and thus might be useful for selection of tolerant plant types under the above conditions of salinity.