• Mycobiology
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• v.39 no.1
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• pp.7-11
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• 2011

Shimizuomyces paradoxus showed no inhibitory effect against plant pathogen fungi, such as Fusarium oxysporum f. sp. lycopersici and Alternaria solani. The S. paradoxus culture filtrate showed higher seed germination and seedling growth rates in canola than distilled water and potato-dextrose broth. A conidial suspension of $1.0{\times}10^4/mL$ resulted in the highest growth stimulating effects on total plant length, and fresh and dry weight of shoots and roots in cucumber, when compared to the highest suspension concentration. Total plant length and shoot weight increased with the foliar spray treatment, and root length and root weight increased by simultaneous treatments of soil drenching and foliar spray in cucumber. Lower concentrations of the S. paradoxus conidial suspension increased the harvest of tomato fruit.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.10a
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• pp.17-17
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• 2010

Rhizobacteria are a diverse group of free-living soil bacteria that live in plant rhizosphere and colonized the root system. Plant growth-promoting rhizobacteria (PGPR) possessing ACC deaminase (ACCD) can reduce ACC and ethylene in plant tissue and mediated the growth of plants under various stresses including salt stress. ACCD decrease ethylene levels in plant tissue that produce high levels of ethylene in tissue via elevated levels of ACC under salt stress. We selected strains of Pseudomonas sp. possessing ACCD activity for their ability to promote plant growth under salt stress from soil sample collected at Byeonsan, Jeonbuk, South Korea. The Pseudomonas strains possessing ACCD increased the rate of the seedling and growth of chinese cabbage seeds under salt stress. We cloned ACCD gene from P.fluorescens and expressed recombinant protein in Escherichia coli. The active form of recombinant ACCD converted ACC to a-ketobutyrate. The in vivo treatment of recombinant ACCD itself increase the rate of the seedling and growth of Chinese cabbage seeds under salt stress. The polyclonal P.fluorescens anti-ACCD antibody specifically reacted with ACCD originated from Pseudomonas. This indicates that the antibody might act as an important indicator for ACCD driven from Pseudomonas exhibiting plant growth-promoting activity. This study will be useful for identification of newly isolated PGPR containing ACCD and exploioting the ACCD activity from PGPR against various biotic and abiotic stresses.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.2
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• pp.72-79
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• 2010

This research was initiated to investigate the revegetation characteristics under seedling types of native woody plants on a disturbed slope. 4 different seedling types of woody plants (control, unit seedling plot, nature seedling plot, pot seedling plot) with the same woody plants (Rhus chinensis Mill, Albizzia julibrissin Duraz., Quercus acutissima Carr., Pinus densiflora S. et Z.) were treated with 3 replications on the experimental disturbed slope. Data such as the died number of native woody plants, growth height and visual quality were analyzed. The died number of plants were high in unit seedling plot when compared to the rest plots of seedling types. From the standpoint of died plant species, the died number were high in Rhus chinensis and Albizzia julibrissin whereas the died number were low in Quercus acutissima and Pinus densiflora. That's because we used 1-year old seedlings of Rhus chinensis and Albizzia julibrissin and 3-year old seedling Quercus acutissima and Pinus densiflora. This result indicated that the died reason of native woody seedling was not species but seedling age. Both growth height and visual quality were ranked first in the pot seedling plot, second in the nature seedling plot, third in the unit seedling plot, and last in the control plot, respectively. However, the difference of both growth height and visual quality in each seedling plot was not observed on the 11 months after planting. Especially, the main reason of low rooting and growth value in the unit seedling plot was due to wooven fabric outer crust of pot that obstruct seedling rooting into the soil. We concluded that pot seedling of native woody plants was effective in the viewpoint of the ecological disturbed slope revegetation.

• Plant Resources
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• v.8 no.2
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• pp.116-121
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• 2005

Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous or methanol extracts and plant residues from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine its allelopathic effects, and the results showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Aqueous leaf extracts of $40g\;L^{-1}$ were completely inhibitory on root growth of alfalfa, while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Leaf residue incorporation at $100g\;kg^{-1}$ into soil on seedling growth of barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) inhibited both shoot and root fresh weights of barnyard grass by 94 and $96\%$, respectively. Methanol extracts from BuOn and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did those from hexane and water fractions. The results based on bioassay of extracts and residues show that X. occidentale had potent an allelopathic activity against other plant species.

• Korean Journal of Medicinal Crop Science
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• v.19 no.4
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• pp.227-232
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• 2011

The roots of Platycodon grandiflorum has been widely used as a crude drug or a food stuff. Unfortunately, the output and the quality is not regular and highly dependent on the cultivation area and cultivation method. Therefore, seedling cultivation study of this plant under structure with various fertilizer supply was performed. As a result, significant big difference between ammonia nitrogen and nitrate nitrogen content was shown in the root at seedling stage while the difference was not significant in the aerial parts. Fresh weight of the root (7.73 g plant$^{-1}$) was higher in the group treated with three major nutrients (N, P and K) than in those treated with three major nutrients and calcium or magnesium or both calcium and magnesium, and non-treated group (2.69 g plant$^{-1}$). Total nitrogen content was recognized to be significantly correlated with root weight, plant height, number of leaves and weight of aerial parts. Ammonium nitrogen content was more correlated the growth of P. grandiflorum than nitrate nitrogen. For phosphoric acid, significant correlation was also shown with the four growth factors.

• The Korean Journal of Ecology
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• v.21 no.2
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• pp.151-156
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• 1998

the phytotoxic effects of volatile substances emitted from the tomato plant (Lycopersicon esculentum) on receptor plants such as Bidens bipinnata, Plantago asiatica, Lactuca sativa, Eragrositis ferruginea and Achyranthes japonica were investigated. Bolatile substances from the leaves inhibited seedling growth of receptor plants in the laboratory tests. The inhibition response varied with the concentration of compounds. To identify the phytotoxic compounds from tomato plant a GC/MS method was employed. Forth compounds, such as trans-2-hexeal, linalool, phenylacetaldehyde, methlsalicylic acid and tetradecanaic acid were identified from the essential oil of tomato plants. The findings suggest that the tomato plant may have allelopathic potential.

• Journal of Plant Biotechnology
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• v.33 no.4
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• pp.263-270
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• 2006

Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid $(GA_3),\;N^6-benzylaminopurine(BA)$, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with $8.88{\mu}M$ BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10a
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• pp.38-42
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• 2003

This experiment was carried out the investigation of proper temperature of germination and growth responses of turf grass to different seedling ages. The results were as follows; 1. It was indicated that the proper temperature of germination was 30$^{\circ}C$ of day and 15$^{\circ}C$ of night, and day length was 14/10 hrs. 2. It was observed that 50 days of seedling was excellent with growth components such as number of tiller, length of tillering, number of node and internode length.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.2
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• pp.121-124
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• 2001

Potato (Solanum tuberosum L.) tubers of 10, 20, 30, 40, and 50 g were planted in plug trays with vermiculite-based root medium on 10 August 2000 and grown for 15 days in a glasshouse to determine the effects of mini-tuber size on plug seedling growth and field performance of plug seedlings. For a control, common potato tubers weighing 50 g were also planted. As size of seed tubers planted increased from 10 to 50 g, seedling height decreased from 24.6 to 20.0cm while shoot number per seedling increased from 2.0 to 3.5, main stem diameter from 4.3 to 6.1mm, and fresh weight of root + top from 9.3 to 19.4 g/seedling. At 90 days after transplanting plug seedlings, the total number of tubers per plant increased from 3.62 to 4.72, average tuber weight from 62.9 to 72.8g, and total tuber yield 20.5 to 23.6 t/ha with increase in seed tuber size. Plug seedlings raised from 50g tubers produced 22% more tubers per plant and had 21 % higher＞80g tuber yield than the directly planted potatoes.

• Korean Journal of Plant Resources
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• v.33 no.1
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• pp.24-32
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• 2020

This study was aimed to establish the most effective approach for the cultivation of Veronica rotunda var. subintegra (Nakai) T.Yamaz. plants, which was expected as new ornamental plants. We conducted an experiment using plug seedlings, varied the seeding container type and seeding rate. We also varied seedling quality, planting container, soil type, and shading ratio. Seedling quality was used seedlings produced from different seeding containers and seeding rates. The seedling quality were seeding growth using 162, 200, and 288 trays, and seedings rate was used seedlings produced by sowing 1, 2, 4 and 6 seeds. As a result, 162 trays of seedlings were suitable for use in this study. Plants grown with one seed per cell in individual cells exhibited increased individual growth, but those grown with four seeds per cell exhibited better growth for the whole plant. According to seedling quality, seedlings produced in the 162 trays or with four seeds per cell showed higher growth than other seedlings. In the cultivation of V. rotunda var. subintegra (Nakai) T.Yamaz., seedling growth increased depending on container capacity for both shoot and root parts. Container material had no significant impact on seedling growth. Seedlings grew the best on horticultural substrate, and showed better growth on mixed soils with high decomposed granite content than on peatmoss.