• Title/Summary/Keyword: plant production system

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A Multi-microbial Biofungicide for the Biological Control against Several Important Plant Pathogenic Fungi (진균성 식물병해 방제를 위한 항생물질 생산 길항미생물의 복합제제화)

  • Jung, Hee-Kyoung;Ryoo, Jae-Cheon;Kim, Sang-Dal
    • Applied Biological Chemistry
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    • v.48 no.1
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    • pp.40-47
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    • 2005
  • In order to develop a multi-microbial biofungicide against several important plant pathogenic fungi, strains were isolated from the phtophthora blight suppressive red-pepper field soil of Gyeongsangbuk-do, Korea. Strains AY1, AY6, AB1, BB2 and F4, which had strong antagonistic ability against Phytophthota capsici and Fusarium oxysporum, were selected for their involvement with strains of biocontrol fungicide. There were no antagonism among the selected strains and were compatible for making the biofungicide. Their antagonistic mechanisms, except for strain BB2, were an antibiosis by the production of antibiotic, while BB2 produced not only an antibiotic but also cellulase as an antagonistic mechanism against blight causing P. capsici. They were identified as Halobacterium sp. AB1, Xenorhadus sp. AY1, Bacillus sp. AY6, Bacillus sp. BB2, Zymomonas sp. F4 by various cultural, biochemical test and $Biolog^{TM}$ System 4.0. The highest levels of antifungal antibiotic could be produced after 48 hrs of incubation under the optimal medium which were 0.1% galactose, 0.1% $NaNO_2$, 5 mM $Na_2{\cdot}HPO_4$ (pH 5.5). The cultured multi-microbial biofungicide showed strong biocontrol activity against bacterial wilt disease and fusarium wilt disease in cucumber and tomato fields.

A Study on the Microflora of the Han River(II) The phytoplanktons and its seasonal variaton in the area of the Chun-chon and the Chung-pyong reservoir of the Han River (한강의 Microflora에 관한 연구 (제2보) 춘천 및 청평정수지를 중심으로 한 한강의 식물성 planktond과 그 계절적 소장)

  • 정영호
    • Journal of Plant Biology
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    • v.11 no.2
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    • pp.1-30
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    • 1968
  • The Han River was investigated monthly from April through September, 1967 at Chun-chon reservoir, Shin-yean, So-yang, Chung-pyong reservoir, Yang-soo-ri and Gi-doo-won to clarify the phytoplankton and to study its seasonal variation. At the same time, vertical distribution of phytoplankton was also studied from June through September at Chun-chon and Chung-pyong reservoir. The results obtained are as follows: As the environmental conditions, water temperature, dissolved oxygen, pH, and transparency were determined. The plankton samples collected from six stations were identified and classified by Engler's classification system. It resulted in 4 phyla, 4 classes, 3 subclasses, 13 orders, 27 families, 5 tribes, 71 genera, 222 species, 1 subspecies, 51 varieties and 2 forms. The total number of phytoplankton identified was 276. Of them, 84 species, 1 subspecies 26 varieties, 2 forms were recorded in this paper for the first time. Considering with the habitat of the total number of phytoplanktons, the highest percentage of Limnoplankton existed in the Chung-pyong reservoir. It suggests that hte Chung-pyong reservoir is becoming to have the character of lake. The planktons appeared commonly during six months at six station belong to Chrysophyta including 22 species in 11 genera. Among these 22 species, Navicula, consists of 6 species, was the most abundant. The number of species identified uniquely at each station were 5 species in the Chun-chon reservoir, 6 in Chung-pyong and Yang-soo-ri, 8 at Shin-yean, 11 at So-yang, and 18 at Gi-doo-won. The number of species occurred at all stations were 29 in spring, 42 in Autumn and during investigation period. The maximum production of total phytoplankton was generally shown in the late spring and the early fall with differences in quantity at six collecting statins. In the Chun-chon reservoir, the high concentration of approximately 1, 190, 000 cells per litter in the months of August and September was due to the increase of Chrysophyte genus, Melosira and the highest concentration of approximately 2, 000, 000 in July at Gi-doo-won was caused Chlorophyte genus, Secenedesmus. The Chun-chon reservoir of three years old was different from the Chung-pyong reservoir of 25 years old with respect to increase in Melosira genus in August and September. After the completion of the Ui-am reservoir, though there was an increase in total density, the number of species of phytoplankton was not changed. It suggests that Ui-am reservoir is similar to the Chun-chon reservoir under the influence of the latter. The density of total phytoplankton was generally high at the depth of 2m to 5m for the two reservoirs.

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Immunomodulatory Effects of Fructus and Semen from Rosa rugosa on Macrophages (해당화의 과육 및 종자추출물의 대식세포 면역조절작용)

  • Kang, Nam-Sung;Sohn, Eun-Hwa
    • Korean Journal of Plant Resources
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    • v.23 no.5
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    • pp.399-405
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    • 2010
  • Rosa rugosa has been used as a folk medicine with various pharmacological properties for a long time in Asia. Recently, it has been reported that the extract of fractions from different parts of Rosa rugosa have various pharmacological effects on diverse diseases including diabetes, inflammatory diseases and tumor. We investigated effects of fructus extracts of Rosa rugosa(RRF) and semen extracts of this herb(RRS) on macrophage to evaluate the possibilities as a biological response modifier. We showed increased effects on tumoricidal activity, phagocytic activity, TNF-$\alpha$ and NO production in RRF-treated groups without direct tumor cell cytotoxicity. RRS-treated groups increased direct tumor cell cytotoxicity at high dose without tumoricial activity except increasing of TNF-$\alpha$ release. These results provide further possibilities for the beneficial immunomodulating effects of RRF on immune system with relatively larger safety margin rather than RRS.

Enhanced Production of hCTLA4Ig through Increased Permeability in Transgenic Rice Cell Cultures (형질전환 벼 현탁세포 배양에서 투과성 증진을 통한 hCTLA4Ig의 생산성 증대)

  • Choi, Hong-Yeol;Cheon, Su-Hwan;Kwon, Jun-Young;Lim, Jung-Ae;Park, Hye-Rim;Kim, Dong-Il
    • KSBB Journal
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    • v.31 no.4
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    • pp.277-283
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    • 2016
  • In this system, rice cells were genetically modified to express human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) using RAmy3D promoter induced by sugar depletion. Even though the target protein fused with signal sequence peptide, plant cell wall can be a barrier against secretion of recombinant proteins. Therefore, hCTLA4Ig can be trapped inside cell wall or remained in intracellular space. In this study, to enhance the secretion of hCTLA4Ig from cytoplasm and cell walls into the medium, permeabilizing agents, such as dimethyl sulfoxide (DMSO), Triton X-100 and Tween 20, were applied in transgenic rice cell cultures. When 0.5% (v/v) of DMSO was added in sugar-free medium, intracellullar hCTLA4Ig was increased, on the other hand, the secreted extracellular hCTLA4Ig was lower than that of control. DMSO did not give permeable effects on transgenic rice cell cultures. And Triton X-100 was toxic to rice cells and also did not give enhancing permeability of cells. When 0.05% (v/v) Tween 20 was added in rice cell cultures, however, intracellular hCTLA4Ig was lower than that of control cultures. And the maximum 44.76 mg/L hCTLA4Ig was produced for 10 days after induction, which was 1.4-fold increase compared to that of control cultures. Especially, Tween 20 at 0.05% (v/v) showed the positive effect on the secretion of hCTLA4Ig though the decrease of intracellular hCTLA4Ig. Also, Tween 20 as a non-toxic surfactant did not affect the cell growth, cell viability and protease activity. In conclusion, secretion of hCTLA4Ig could be increased by enhancing permeability of cells regardless of the cell growth, cell viability and protease activity.

Occurrence of Fusarium Wilt Caused by Fusarium oxysporum f. sp. lactucae and Cultivar Susceptibility on Lettuce (Fusarium oxysporum f. sp. lactucae에 의한 상추 시들음병 발생 및 품종간 감수성 비교)

  • Kim, Jin-Young;Hong, Sun-Sung;Lee, Jin-Gu;Lee, Hyun-Ju;Lim, Jae-Wook;Kim, Jin-Won;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.14 no.2
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    • pp.79-84
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    • 2008
  • A wilt disease on lettuce was observed in 2006 and 2007 in commercial plastic house at main production areas of lettuce in Icheon, Yongin and Goyang of Gyeonggi Province. The disease was characterized by the wilting of lower leaves, accompanied by stunting symptoms of the whole plants. Old affected stems showed the black streak in the vascular system. The pathogen, Fusarium oxysporum f. sp. lactucae was isolated from stems and roots of diseased plants. Isolated pathogen also produced the microconidia and macroconidia with chlamydospores on carnation leaf agar medium. The pathogen easily invaded and made many chlamydospores on the roots of lettuce and also made dark streaking through the vascular in the lettuce stems. The density of Fusarium sp. in the severely diseased field soil was more higher populations than that in the healthy and less diseased field soil. The minimum population of pathogen would be above $10^3$cfu/g soil to induce the Fusarium wilt on lettuce in plastic house. The results of pathogenicity test showed 'Sunpung' and 'Mipungpochap' was high susceptible to Fusarium pathogen isolates while some cultivar 'Mihongjeokchukmyeon' and 'Jinjachukmyeon' showed moderate resistance. Disease development for some lettuce was related to treated temperature, so the symptom was more severe above $25^{\circ}C$. Selection of appropriate lettuce cultivar and planting time should be related for the successful control of Fusarium wilt.

Effect of Seed Imbibition into Water and Acetic Acid Solution on its Floating Rate and Growth of Soybean Sprouts (수침(水浸)과 Acetic Acid 처리에 따른 콩나물의 생장과 형태 변화)

  • Jeon Byong-Sam;Hong Dong-Oh;Kim Hong-Young;Lee Chang-Woo;Kang Jin-Ho
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.204-208
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    • 2006
  • Soybean sprout decay during its culture should be one of serious problems. The study was carried out to clarify the effect of water imbibition and acetic acid treatments on growth and morphological characters of the soybean sprouts. The soybean seeds of 3 cultivars (cv. Eunhakong, Pungsannamulkong and Junjery) imbibed in pure water or 0.l% acetic acid for 3 minutes before soaked for 6 hours into 1 ppm BA solution and aerated for 3 hours immediately before 6 day culture. On the 6th day, the sprouts were classified into 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm, > 4cm and not germinated and seed floating rate, their morphological characters, fresh fraction weights and productivity were measured. The best water imbibition for seed cleaning was to soak the seeds for 5 minutes and then to aerate soak them for 40 minutes. In Pungsannamulkong and Junjery, percentage of the sprouts with hypocotyls of longer than 4 cm was higher in water imbibition than in acetic acid treatment for seed disinfection although in Eunhakong there was no significant difference between the two treatments. Eunhakong and Junjery had greater lateral root formation rate and its number per sprout in water imbibition than in acetic acid treatment but Pungsannamulkong showed reverse result. Eunhakong and Pungsannamulkong, furthermore, had more total fresh weight in acetic acid treatment than in water imbibition but Junjery showed reverse result, although there was no significant difference between the two treatments in productivity of mass production system.

Fruit Color Improvement by ABA Treatment and Determination of Harvesting Time in 'Hongisul' Grapes (ABA 처리를 통한 '홍이슬' 포도의 착색향상 및 적정 수확기 설정)

  • Shin, Kyoung-Hee;Park, Hee-Seung
    • Horticultural Science & Technology
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    • v.30 no.3
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    • pp.256-260
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    • 2012
  • This study was carried out to improve the fruit color through ABA treatment and to determine the optimum harvest time for producing high quality fruits in 'Hongisul' grapes. Spraying of $1000 mg{\cdot}L^{-1}$ exogenous ABA at early verasion (70 days after full bloom, DAFB) brought increase of endogenous ABA and soluble solid contents and enhanced fruit coloration. So, it was possible to harvest ABA treated fruits from 85 days after full bloom (15 days after treatment) by accelerated anthocyanin content which increased continuously until 110 DAFB. An increase of soluble solid and decrease of total acidity appeared steadily with the onset of berry ripening. After 100 DAFB, soluble solid content and total acidity did not change significantly, but the berry firmness was suddenly decreased. Consequently, it was suggested that ABA treated fruits need to be harvested at about 100 DAFB because of their short period of marketing by over ripening. On the contrary, harvesting of untreated fruit was totally impossible at 85 DAFB because of their poor berry coloration. But it was possible to harvest them at 100 DAFB based on the soluble solid/acidity ratio, whereas the berry coloration was progressed poorly. On the other hand, the fruits harvested at 110 DAFB showed acceptable berry coloration but their berry firmness was dropped significantly coincide with overripening. Therefore, it was needed to develop an altered production system for improving coloration at around 100 DAFB in 'Hongisul' grapes.

Substitution of Plant and Animal Proteins for Fish Meal in the Growing Korean Rockfish (Sebastes schlegeli) Feeds (조피볼락 육성용 사료의 어분 대체원으로서 식물성 및 동물성 단백질 혼합 첨가 효과)

  • LEE Sang-Min;JEON Im-Gi;LEE Jong-Yun;PARK Sung-Real;KANG Yong-Jin;JEONG Kwan-Sik
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.5
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    • pp.651-662
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    • 1996
  • A 15-week growth trial was conducted in flow-through aquarium system to develop practical feeds for growing Korean rockfish. Two replicate groups of the fish averaging 114 g were fed one of nine diets containing $45\~50\%$ crude protein from various practical ingredients such as fish meal, meat meal, feather meal, blood meal, soybean meal, corn gluten meal, and wheat flour with or without supplemental essential amino acids (EAA) or enzyme mixture. The dietary EAA were adjusted by considering EAA composition of each dietary protein source, A/E ratio (each essential amino $acid\times1000/total$ essential amino arid including Cys and Tyr) calculated using Ah composition of the Korean rockfish whole body and the EAA requirement of other fish. Results indicate that animal and plant protein sources could substitute for fish meal up to $50\%$ in the diets, and the supplementation of amino acids and enzyme mixture have no beneficial effects on fish performance. Fish growth, body composition, nutrient utilization, and cost of fish production are discussed in relation to nutritional values of the protein sources used in diets.

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Colors and Sizes of Insect Screen Net Influence Physical Control of Bemisia tabaci and Frankliniella occidentalis under Controlled Environments (환경제어 조건에서 방충망 색과 크기가 담배가루이 및 꽃노랑총채벌레의 물리적 방제에 미치는 영향)

  • Jung, Chung-Ryul;Yoon, Jung-Beom;Kim, Kwang-Ho;Lee, Guang-Jae;Heo, Jeong-Wook;Kim, Hyun-Hwan
    • Korean Journal of Environmental Agriculture
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    • v.35 no.1
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    • pp.46-54
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    • 2016
  • BACKGROUND: The tobacco whitefly(Bemisia tabaci Gennadius) and western flower thrips(Frankliniella occidentalis Pergande) seriously damaged to several greenhouse crops and transmitted plant viruses such as the Tomato Yellow Leaf Curl Virus(TYLCV) and Tomato Spotted Wilt Virus(TSWV). Objective of this study was to elucidate exclusion effects of insect screen nets by various hole sizes and colors for control of the two insect pests in controlled environments such as a closed plant production system.METHODS AND RESULTS: The exclusion effects to various hole sizes of three other colors with 30 individuals of two insect pests was evaluated. B. tabaci was not showed not difference to different colors and sizes. F. occidentalis showed that 0.2 mm black screen was the most effective exclusion than other colors of 0.6 and 0.8 mm.CONCLUSION: The two insects were different reponses to various hole sizes of white and other color screen nets. It was proved that the 0.4 mm white screen net used in this experimental condition was suitable for exclusion of B. tabaci and 0.2 mm black forF. occidentalis.

Action mechanism of upstream open reading frame from S-adenosylmethionine decarboxylase gene as a in vivo translational inhibitor (S-Adenosylmethionine decarboxylase 유전자의 upstream open reading frame이 in vivo에서 translational inhibitor 로서의 작용 기작)

  • Choi, Yu-Jin;Park, Ky-Young
    • Journal of Plant Biotechnology
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    • v.38 no.1
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    • pp.87-93
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    • 2011
  • S-Adenosylmethionine decarboxylase (SAMDC; EC 4.1.4.50), a key enzyme for polyamines biosynthesis, was tightly regulated for homeostatic levels. Carnation SAMDC gene (CSDC9) has an small upstream open reading frame (uORF) of 54 amino acids in 5'-leader sequence. To explore the functional mechanism of uORFs in controlling translation, we used a GUS reporter gene driven with the 35S promoter and uORF region of SAMDC gene for making transgenic tobacco plants. In our experiment, there were a translational inhibition of its downstream GUS ORF by SAMDC uORF sequence or SAMDC uORF protein. Expecially, translational inhibition was most effective in point-mutated construct, in which the start codon was changed. Therefore, this results suggested the ribosomal stalling might be involved in this translational inhibitory process. The frame shift in amino acid sequence of SAMDC uORF with start codon and stop codon resulted in a moderate increasing in GUS activity, suggesting the native amino acid sequence was important for a function as a translational inhibitor. Also, we showed that the production of GUS protein was significantly inhibited in the presence of the small uORF using histochemical analysis of GUS expression in seedlings and tobacco flowers. Importantly, the small uORF sequence induced a real peptide of 5.7 kDa, which was provided the presence of SAMDC uORF peptide band using an in vitro transcription/translation system. The peptide product of uORF might interact with other components of translational machinery as well as polyamines, which was resulted from that polyamine treatment was inhibited GUS protein band in SDS-PAGE experiment.