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A Multi-microbial Biofungicide for the Biological Control against Several Important Plant Pathogenic Fungi  

Jung, Hee-Kyoung (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
Ryoo, Jae-Cheon (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
Kim, Sang-Dal (Department of Applied Microbiology, College of Natural Resources, Yeungnam University)
Publication Information
Applied Biological Chemistry / v.48, no.1, 2005 , pp. 40-47 More about this Journal
Abstract
In order to develop a multi-microbial biofungicide against several important plant pathogenic fungi, strains were isolated from the phtophthora blight suppressive red-pepper field soil of Gyeongsangbuk-do, Korea. Strains AY1, AY6, AB1, BB2 and F4, which had strong antagonistic ability against Phytophthota capsici and Fusarium oxysporum, were selected for their involvement with strains of biocontrol fungicide. There were no antagonism among the selected strains and were compatible for making the biofungicide. Their antagonistic mechanisms, except for strain BB2, were an antibiosis by the production of antibiotic, while BB2 produced not only an antibiotic but also cellulase as an antagonistic mechanism against blight causing P. capsici. They were identified as Halobacterium sp. AB1, Xenorhadus sp. AY1, Bacillus sp. AY6, Bacillus sp. BB2, Zymomonas sp. F4 by various cultural, biochemical test and $Biolog^{TM}$ System 4.0. The highest levels of antifungal antibiotic could be produced after 48 hrs of incubation under the optimal medium which were 0.1% galactose, 0.1% $NaNO_2$, 5 mM $Na_2{\cdot}HPO_4$ (pH 5.5). The cultured multi-microbial biofungicide showed strong biocontrol activity against bacterial wilt disease and fusarium wilt disease in cucumber and tomato fields.
Keywords
biocontrol; microbial agent; Phytophthota capsici; Fusarium oxysporum;
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1 Lee, E. T. and Kim, S, D. (2001) An antifungal substance, 2,4-diacetylphloroglucinol, produced from antagonistc bacterium Pseudomonas fluorencens 2112 against Phytophthora capsici. Kor. J. Microbiol. Biotechnol. 29, 37-42
2 Kim, K. Y. and Kim, S. D. (1997) Biological control of Pyricularaia oryzae blast spot with the antibiotic substance produced by Bacillus sp. KL-3. Kor. J. Microbiol. Biotechnol. 25, 396-402
3 Lim, H. S. and Kim, S. D. (1994) The production and enzymatic properties of extracellular chitinase from Pseudomonas stutzeri YPL-1, as a biocontol agent. J. Microbiol. Biotecnol. 4, 134-140
4 Lim, H. S. and Kim. S. D. (1994) The production and enzymatic properties of extracellular chitinase from Pseudomonas stutzeri YPL-1, as a biocontol agent. J. Microbiol. Biothechnol. 4, 134-140
5 Takeuchi, S., Hirayama, K., Ueda, K., Sasaki, H., and Yonehara., H., (1958) Blasticidin S, a new antibiotic. J. Antibiot. 11, 1-5
6 Paulitz, T. C., and Loper, J. E. (1991) Lack of a role for fluorescent siderophore production in the biological control of Phythium damping-off of cucumber by a strain of Pseudomonas putida. Phytopathology 81, 930-935   DOI
7 Kim, D. W., Do, K. S. and Choi, S. W. (2001) Antagonistic search for biological control of fusarium wilt in cymbium genus. J. Kor. Hort. Sci. 42, 581-586
8 Lim, H. S. and Kim, S. D. (1990) Antifungal mechanism of Pseudomonas stutzeri YPL-1 for biocontrol of Fusarium solani causing plant root rot. J. Microbiol. Biotechnol. 18, 81-88
9 Harman, G. E., Chet, I. and Barker, R. (1980) Tricoderma hamatum effects on seed and seedling disease induced in radish and pea by Phythium spp. or Rhizoctonia solanl. Phytopathology 70, 1167-1172   DOI
10 Ordentlich, A., Elad, Y. and Chet, I. (1988) The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii. Phytopathology 78, 84-88
11 Tominaga, Y. and Tsujisaka, Y. (1976) Purification and some prosperities of two chitinase from Streptomyce sorientalis which lyse Phizopus cell wall. Agric. Biol. Chem. 40, 2325-2333
12 Tom, R. A. and Carroad, P. A. (1998). Effect of reaction condition on hydrolysis of chitin by Serratia marcesens QMB 1466 chitinase. J. Food Sci. 46, 646-647   DOI
13 Yun, K. H., Lee, E. T. and Kim, S. D. (2001) Identificaton and antifungal antagonism of Chryseomonas luteola 5042 against Phytophthora capsici. Kor. J. Microbiol. Biotechnol. 29, 186-193
14 Hong, S. H., Lam, J. S., Park, Y. B. and Ha, J. H. (1990) The optimum culture condition for the production of antibiotics KG-1167B produced by Clostrium sp. Kor. J. Microbiol. Biotechnol. 18, 292-265
15 Hadar, Y., Chet, I. and Heins, Y. (1979) Biological control of Rhizoctonia solani damping-off with wheat bran culture of Trichoderma harizianum. Phytopathology 69, 64-68   DOI
16 Paik, S. B. and Kim, D. W. (1995) Screening for phyllospheral antagonisitc microorganism for control of Red-pepper anthracnose. Kor. J. Mycol. 23, 190-195
17 Watanabe, T., Oyanagi, W., Suzuki, K. and Tanaka, H. (1990) Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation. J. Bacteriol. 172, 4017-4022
18 Holt, J. G., Krieg, N. R., Sneath, P. H. Staley, J. T. and Williams, S. T. (1994) In Bergey's manual of determinative bacteriology. (9th ed.), Williams Wikins
19 Okazaki, K., Kato, F., Watanabe, N., Yasuda, S., Masui, Y. and Hayakawa, S. (1995) Purification and properties of two chitinase from Streptomyces sp. J-13-3. Biosci. Biotech. Biochem. 59, 1586-1587   DOI   ScienceOn
20 Leoffler, W. Tschen, J. S., Venittanakom, N., Kugler, M., Knorpp, E., Hsieh, T. F. and Wu, T. G. (1986) Antifungal effects of bacilysin and fengymycin from Bacillus subtilis F-29- 3: a comparison with activaties of other Bacillus antibiotics. J. phytopathology 115, 204-213   DOI
21 Kim, Y. S., Song, J. K., Moon, D. C. and Kim, S. D. (1997) Isolation and structure determination of antifungal antibiotics from Bacillus subtilis YB-70, a power biocontrol agent. Kor. J. Microbiol. Biotechnol. 25, 62-37