• Journal of Plant Biology
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• v.30 no.1
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• pp.1-9
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• 1987

Leaf mesophyll protoplasts of Nicotiana tabacum (nitrate reductase deficient mutant) were fused with cell suspension protoplasts of albino Petunia inflata in an electric field. Hybrid cell colonies were selected for nitrate reductase proficiency and chlorophyll synthesis. Five hybrid plant lines, regenerated from the selected calli lines, were analysed by electrophoresis, number of chromosomes and morphological characters. Somtic hybrid plants showed both parent patterns in the isozymesof isoleucine aminopeptidase and esterase. The hybrids had the expected chromosome number of 62 and exhibited an intermediate floral morphology when compared with the parents, but plant height and leaf arrangement were similar to N. tabacum.

• Proceedings of the Botanical Society of Korea Conference
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• 1999.04a
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• pp.6-6
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• 1999

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.178-178
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• 2003

• Proceedings of the Zoological Society Korea Conference
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• 2001.10a
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• pp.118.1-118
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• 2001

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.103.3-104
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• 2000

No Abstract, See Full Text

• Journal of Plant Biology
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• v.30 no.3
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• pp.215-223
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• 1987

Pollen grains of six geners and six species from Korean Theaceae were observed with light and scanning electron microscopes. Pollen morphology divided the investigated species into the tricolporoidate Theoideae and the tricolporate Ternstroemiodideae. On the basis of surface sculpturing pattern, further palynological relationships and classificatin were understood. Some of the nomenclatural problems were discussed along with the palynological results.

• Journal of Forest and Environmental Science
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• v.31 no.3
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• pp.214-224
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• 2015

The economy of India and so also of many Asian countries depends on bamboos and their uses are not only in domestic items but also in rural housing and raw materials to several industries and germplasm characterization is an important link between the conservation and utilization of plant genetic resources. Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering coupled with different biotic agencies and environmental factors. Identification and genetic relationships among accessions of Thamnocalamus falconeri were investigated using morphology and random amplified polymorphic DNAs (RAPD) technique. Analysis started by using 51 vegetative characters and forty two 10-mer primers that allowed us to distinguish different genotypes hailing from different eco- zones of Garhwal Himalayas (India). The selected primers (12) were used for identification and for establishing a profiling system to estimate genetic diversity. A total of 79.33% polymorphism was estimated by using 12 selected primers. The genetic similar analysis was conducted based on binary digits i.e. presence (1) or absence (0) of bands, which revealed a wide range of variability among the species whereas genetic relatedness was quite high based on vegetative characters. Cluster analysis clearly showed two major clusters for both of the markers viz. morphology and RAPD belonging to 10 accessions of T. falconeri. Two major clusters were further divided into minor clusters. Cluster based on RAPD marker showed grouping of accessions of closed locality whereas analogy was reported for vegetative traits. The RAPD technique has the potential for use in species identification and genetic relationships studies of bamboo for breeding program.

• Korean Journal of Plant Taxonomy
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• v.33 no.4
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• pp.411-420
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• 2003

To examine the seed morphology in Korean Orobanchaceae, seeds of 5 species including 1 variety in five genera and one related taxon, Boschniakia himalaica Hook. f. & Thomson in Hook. f. from the Himalaya (7 taxa in total) were investigated by scanning electron microscopy (SEM). Two different seed types are recognized depending on three characters (e.g, .the seed size, seed coat structure and seed number per fruit). - Type I: size less than 1mm, seed coat structure reticulate and seed number per fruit more than ca. 1,000; Type II: size more than 1 mm, exotesta irregularly crested and seed number per fruit less than 1,000 (Lathraea japonicu Miq.). In Type I, three subtypes of exotesta internal surface pattern can also be classified; (1) Subtype Ia: reticulate - include irregularly striate (Ia') and deeply reticulate (Ia"), (2) Subtype Ib: foveolate, (3) Subtype Ic: smooth. Systematics application of the seed morphological data for the Korean Orobanchaceae is also briefly discussed.

• Carbon letters
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• v.13 no.3
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• pp.161-166
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• 2012

Carbon materials were synthesized by pyrolysis from fibers of Corn-straw (Zea mays), Rice-straw (Oryza sativa), Jute-straw (Corchorus capsularis) Bamboo (Bombax bambusa), Bagass (Saccharum officinarum), Cotton (Bombax malabaricum), and Coconut (Cocos nucifera); these materials were characterized by scanning electron microscope, X-ray diffraction (XRD), and Raman spectra. All carbon materials are micro sized with large pores or channel like morphology. The unique complex spongy, porous and channel like structure of Carbon shows a lot of similarity with the original anatomy of the plant fibers used as precursor. Waxy contents like tyloses and pits present on fiber tracheids that were seen in the inherent anatomy disappear after pyrolysis and only the carbon skeleton remained; XRD analysis shows that carbon shows the development of a (002) plane, with the exception of carbon obtained from bamboo, which shows a very crystalline character. Raman studies of all carbon materials showed the presence of G- and D-bands of almost equal intensities, suggesting the presence of graphitic carbon as well as a disordered graphitic structure. Carbon materials possessing lesser density, larger surface area, more graphitic with less of an $sp^3$ carbon contribution, and having pore sizes around $10{\mu}m$ favor hydrogen adsorption. Carbon materials synthesized from bagass meet these requirements most effectively, followed by cotton fiber, which was more effective than the carbon synthesized from the other plant fibers.

• Korean Journal of Plant Resources
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• v.8 no.3
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• pp.223-235
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• 1995

Comparative studies on external morphology and anatomical characters were conducted understand the interspecific relationships and possibly to identify the position of Hanabusaya asiatica as an endemic genus among 4 genera and 9 species in H. asiatica and its allied groups. Such morphological characteristics as root shape, leaf size, presence or absence of wings in petiole, corolla and calyx shape, and inflorescence and stigma shape were useful qualitative characters. The morphology of stem, peduncle, calyx lobe, leaf, petiole, and ovary were also good characters to be included in this identification study. In the anatomical observations in these organs, projecting degree of epidermis and number of projecting area rather than cross-sectioned internal structures were found to be valuable index for the classification among species. External morphology and anatomical characters support the position of H. asiatica as an endemic genus very well. Known as the most important external characters to taxonomists(Fedorov, 1957: Nakai, 1952: Hong, 1983), leaf arrangement, inflorescence, and corolla shape were also preven to be very useful characters to identify the genera and species in the H. asiatica and its allied groups.