• 제목/요약/키워드: plant growth promoting effect

검색결과 157건 처리시간 0.036초

Bacillus subtilis C4와 B. cereus D8에 의한 유채의 생육증대 및 무름병과 균핵병 방제효과 (Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot)

  • 이재은;이서현;박경수;박진우;박경석
    • 농약과학회지
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    • 제13권4호
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    • pp.275-282
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    • 2009
  • 선발된 PGPR 균주인 Bacillus subtilis C4와 Bacillus cereus D8 균주의 유채에 대한 생육촉진 및 무름병균인 Erwinia carotovora와 균핵병균인 Sclerotinia sclerotiorum에 대한 방제효과를 검정하기 위하여 실내검정과 온실검정을 실시하였다. 실내검정 결과, C4와 D8균주처리에 의하여 유채의 생육이 40.3%~74% 증가하였으며 무름병이 대조구에 비하여 80% 감소하였다. 실내검정에서 C4와 D8균주를 종자에 처리하였을 때 뿌리가 크게 신장되었다. 주요 식물병원균 Sclerotinia sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici, Colletotrichum acutatum에 대하여 항균활성시험을 수행한 결과 두 균주 중 C4균주는 모든 병원성 곰팡이에 대하여 항균활성을 나타내었다. 온실검정에서 C4와D8균주처리는 대조구에 비하여 유채의 초장, 엽폭 및 엽장을 19.5%~24.9%, 11.3%~15.3%, 14.1%~20.7% 각각 증가시켰으며 균핵병균인 Sclerotinia sclerotiorum에 대한 억제효과가 우수하였다. 이와 같은 결과를 볼 때 C4, D8 균주 처리는 유채의 생육을 촉진시키며 유채에 저항성을 유도하므로 친환경생물방제에 적용할 수 있을 것으로 생각된다.

Enterococcus faecium LKE12 Cell-Free Extract Accelerates Host Plant Growth via Gibberellin and Indole-3-Acetic Acid Secretion

  • Lee, Ko-Eun;Radhakrishnan, Ramalingam;Kang, Sang-Mo;You, Young-Hyun;Joo, Gil-Jae;Lee, In-Jung;Ko, Jae-Hwan;Kim, Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • 제25권9호
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    • pp.1467-1475
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    • 2015
  • The use of microbial extracts containing plant hormones is a promising technique to improve crop growth. Little is known about the effect of bacterial cell-free extracts on plant growth promotion. This study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which Enterococcus faecium LKE12 enhances plant growth in oriental melon. A bacterial strain, LKE12, was isolated from soil, and further identified as E. faecium by 16S rDNA sequencing and phylogenetic analysis. The plant growth-promoting ability of an LKE12 bacterial culture was tested in a gibberellin (GA)-deficient rice dwarf mutant (waito-C) and a normal GA biosynthesis rice cultivar (Hwayongbyeo). E. faecium LKE12 significantly improved the length and biomass of rice shoots in both normal and dwarf cultivars through the secretion of an array of gibberellins (GA1, GA3, GA7, GA8, GA9, GA12, GA19, GA20, GA24, and GA53), as well as indole-3-acetic acid (IAA). To the best of our knowledge, this is the first study indicating that E. faecium can produce GAs. Increases in shoot and root lengths, plant fresh weight, and chlorophyll content promoted by E. faecium LKE12 and its cell-free extract inoculated in oriental melon plants revealed a favorable interaction of E. faecium LKE12 with plants. Higher plant growth rates and nutrient contents of magnesium, calcium, sodium, iron, manganese, silicon, zinc, and nitrogen were found in cell-free extract-treated plants than in control plants. The results of the current study suggest that E. faecium LKE12 promotes plant growth by producing GAs and IAA; interestingly, the exogenous application of its cell-free culture extract can be a potential strategy to accelerate plant growth.

Profiles of Bacillus spp. Isolated from the Rhizosphere of Suaeda glauca and Their Potential to Promote Plant Growth and Suppress Fungal Phytopathogens

  • Lu, Ping;Jiang, Ke;Hao, Ya-Qiao;Chu, Wan-Ying;Xu, Yu-Dong;Yang, Jia-Yao;Chen, Jia-Le;Zeng, Guo-Hong;Gu, Zhou-Hang;Zhao, Hong-Xin
    • Journal of Microbiology and Biotechnology
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    • 제31권9호
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    • pp.1231-1240
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    • 2021
  • Members of the genus Bacillus are known to play an important role in promoting plant growth and protecting plants against phytopathogenic microorganisms. In this study, 21 isolates of Bacillus spp. were obtained from the root micro-ecosystem of Suaeda glauca. Analysis of the 16S rRNA genes indicated that the isolates belong to the species Bacillus amyloliquefaciens, Bacillus velezensis, Bacillus subtilis, Bacillus pumilus, Bacillus aryabhattai and Brevibacterium frigoritolerans. One of the interesting findings of this study is that the four strains B1, B5, B16 and B21 are dominant in rhizosphere soil. Based on gyrA, gyrB, and rpoB gene analyses, B1, B5, and B21 were identified as B. amyloliquefaciens and B16 was identified as B. velezensis. Estimation of antifungal activity showed that the isolate B1 had a significant inhibitory effect on Fusarium verticillioides, B5 and B16 on Colletotrichum capsici (syd.) Butl, and B21 on Rhizoctonia cerealis van der Hoeven. The four strains grew well in medium with 1-10% NaCl, a pH value of 5-8, and promoted the growth of Arabidopsis thaliana. Our results indicate that these strains may be promising agents for the biocontrol and promotion of plant growth and further study of the relevant bacteria will provide a useful reference for the development of microbial resources.

Bacillus sp. AB02를 이용한 식물 병원균에 대한 생물검정 (Biological Control of Plant Pathogens by Bacillus sp. AB02.)

  • 김근기;김용철;최영환;신택순;박기도;강위금;최용락;박현철
    • 생명과학회지
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    • 제18권6호
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    • pp.858-864
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    • 2008
  • 겨울철 과채류 시설 재배 단지에서는 저온 다습하여 균핵병 발생이 많아 피해가 심각하기 때문에, 미생물을 이용한 생물학적 방제법으로 균핵병을 방제하고자 길항균을 분리하였다. 시설 재배 토양으로부터 길항세균 55종을 분리하였으며, 분리한 길항세균 중에 가장 활성이 뛰어난 균주를 AB02 균주로 명명하고 이를 이용하여 식물병원균 5종에 대한 항균 스펙트럼을 조사하였다. 그 결과 잿빛곰팡이병원균인 Botrytis cinerea와 균핵병원균인 Sclerotinia sclerotiorum에 항균효과가 뛰어났다. AB02 균주의 균핵병에 대한 생물학적 방제실험은을 위해 들깨 pot 실험으로 병발생 억제효과와 병 방제효과를 조사했다. 그 결과 균핵병 발생 억제효과는 40%로 나타났고, 병 방제효과는 62%로 아주 높게 나타났다. AB02 균주의 식물 생육 촉진 효과는 전장이 120%, 생체중량이 141% 증가되었으며, 엽수와 엽면적은 각각 121%와 185%의 생육 촉진 효과를 보였다. 그리고 뿌리 생육조사에서는 생체 중량이 무처리 보다 327% 증가하였다. 항균활성과 식물 생육 촉진 활성을 갖는 AB02 균주를 동정한 결과, Bacillus sp.로 확인되었다.

식물 생장촉진 미생물의 외부 유전자 도입과 그 접종효과 (Transfer of Bacillus thuringiensis toxin gene into Bacillus subtilis and its inoculation effects)

  • 이영환;김광식;김용웅;김영일
    • Applied Biological Chemistry
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    • 제35권5호
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    • pp.361-366
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    • 1992
  • 토양전염 병원성 사상균인 Fusarium oxyporum, Rhizoctonica solani에 대하여 길항력을 갖는 균주 Bacillus subtilis를 근권 토양에서 분리하여 동정한 후 이들 균주의 사상균에 대한 길항력, 발아율 및 작물의 생육에 미치는 영향력을 검토하였다. 또한 이 균주의 chromosome에 Bacillus thuringiensis(BT) 독소유전자를 삽입하여 균주의 형질변환을 유도하였다. BT 독소 유전자는 southern blotting에 의하여 확인되었으나 이의 최종 생성물인 독소 단백질은 SDS-PAGE에 확인되지 않았다. 이들 형질변환된 균주의 생리 및 생화학적 특성을 조사한 결과 모균주와 차이는 없었으며, BT 독소유전자가 삽입된 균주는 선충의 유충에 대한 생물학적 검정에서는 효과가 인정되지 않았으나 누에에 있어서는 1X 균체 희석액에서 10내지 20% 정도의 치사율이 관찰되었다. 모균주와 BT 독소유전자에 의하여 형질변환된 균주 모두 발아율 및 작물의 생육을 향상시켰다.

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Effect of the Microalga Chlorella fusca CHK0059 on Strawberry PGPR and Biological Control of Fusarium Wilt Disease in Non-Pesticide Hydroponic Strawberry Cultivation

  • Kim, Min-Jeong;Shim, Chang-Ki;Ko, Byong-Gu;Kim, Ju
    • Journal of Microbiology and Biotechnology
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    • 제30권5호
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    • pp.708-716
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    • 2020
  • The purpose of this study was to identify strawberry wilt pathogens and evaluate the efficacy of Chlorella fusca CHK0059 for improving plant growth and suppressing Fusarium wilt. We identified 10 isolates of wilt pathogens of non-pesticide Seolhyang strawberry plant, including Fusarium oxysporum f. sp. fragariae, using morphological and molecular analysis. On the 15th day after 0.4% CHK0059 treatment, the plant height of the untreated control strawberry plants was significantly greater than that of the CHK0059-treated strawberry plants. After 85 days, both treatments showed a similar tendency regarding the height of the strawberry plants. However, the thickness of strawberry leaves treated with the CHK0059 was found to be 1 mm thicker than that of the untreated control. The flowering percentage of the CHK0059 plants was also 40.2% higher on average than that of the untreated control. The chlorophyll content of strawberry leaves treated with the CHK0059 was also, on average, 6.63% higher than that of the untreated control. After 90 days of the CHK0059 treatment, the incidence of Fusarium wilt in the CHK0059-treated plants had reduced by 9.8% on average compared to the untreated control. The population density of F. oxysporum f. sp. fragariae was also reduced by approximately 86.8% in the CHK0059-treated plants by comparison to the untreated control at 70 days after treatment. The results indicate that the microalga C. fusca CHK0059 is an efficient biological agent for improving strawberry plant growth and suppressing Fusarium wilt disease in organic strawberries.

관상식물 삽목발근에 있어서 NAA, IBA 및 Ethychlozate의 발근촉진효과와 그 생리학적연구 (The Promotive Effect of NAA, IBA and Ethychlozate on Rooting Cuttings of Certain Ornamental Plants and Some Physiological Studies.)

  • 정해준;곽병화
    • 자연과학논문집
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    • 제1권
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    • pp.115-198
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    • 1987
  • The present studies were undertaken to elucidate the influence of auxins, auxin-like substance-ethychlozate ("Figaron"),and pH and sort of rooting media on rooted propagation of certainornamental woody plant cuttings, and to see possible changes in internal compositions characterizing after root-promoting treatment as the cutting stage proceeded. The experimental check-up srevealed and summarized as seen in the following;I. Effect of three different auxin treatments on rooting cuttings: 1) Promotive influence of auxin varied according to different concentration levels, hours of dipping treatment of the auxins, and kind of plants. The greatest effect was obtained for Forsythia ksreana with NAA and IBA, for Ligustrurn obtusifolium var. variegatum with NAA and ethychlozate, for Hydrangea macrophylla, Magnolia kobus, and Magnolia liliflora with NAA, lBA and ethychlozate also. The most effective level of the promotive agents was found 200mg/l for NAA, 1000mg/l for IBA, and 200mg/l for ethychlozate. For Weigela florida and Gardenia jasminoides, range of the most effective level was shown relatively wide spread. 2) NAA was more effective at its optimal level of the rooting agent than ethychiozate for Weigela florida, Viburnum awabuki, Forsythia koreana, Acer palmatum 'Nomura', Bouga invillea glabra, Elaeagnus umbellata, Prunus tomentosa, Ligustrum obtusifolium, Pyracantha coccinea, Cestrum noctu rnum, Hydrangea macrophylla, Codiaeum variegatum, Rhododen dron lateritium, and Ilex crenata var. macrophylla, and yet ethychlozate was found either as equally as effective or more so than NAA for Zebrina pendula, Hibiscus syriacus, Fatshedera lizei, Schefflera arboricola, Campsis grandiflo ra, Ixora chinensis, Euonymus japonica, and Magnolia liliflora. On the contrary, no the auxin effect was noted with Lagerstroemia indica, Trachelospermum asiaticum, and Syringa vulgaris. This probably indicates that these species are genetically different for the auxin response.II. Effect of different pH and sorts of cutting media on rooting cuttings: 1) Bougainvillea showed best in rooting for the number and dry weight at pH 6.5, more with ethychlozate than NAA, while Ligustrum did at pH 5.0 more with NAA than ethychlozate. pH 4.0 medium resulted in the best rooting for Rhododendron with NAA, more than ethychlozate. 2) Use of cutting medium with peat: perlite: vermiculite = 1:1:1 showed to give the greatest rooting percent and dry weight, apart from considering the number of roots. This apparently meant the fact that cutting medium has more to do with root growth than root differentiation. Rhododendron yet showed results with cutting media that use of peat: perlite = 2:1 mixed is more effective on rooting than using peat alone.III. Effect of auxinic treatments on rooting cuttings and change in some cutting compositions: 1) Under the climatic conditions of July having temperature $26.3\pm$$2.4^{\circ}C$for cutting bed, new roots of Magnolia started to show up generally 20 days after the cutting was made, whereas Cestrum did much earlier than that, namely 14 days after. 2) Although total carbohydrate content of Magnolia cuttings showed no marked change without auxin treatment, it did so with the treatment, especially 30 days after the start of cutting. Cestrum cuttings demonstrated a gradual in crease in total carbohydrate content as rooting took place, and the content became reduced more with auxin than with out, just about when rooting proceeded to 14 days after the start of cutting. 3) Magnolia generally showed an increase in total nitrogen content as rooting proceeded more, and Cestrum showed a decrease in total nitrogen of cuttings. The auxin treatment exhibited no pertinent relation with change in plant nitro gen when rooting is promoted with auxin treatment. 4) An abrupt drop of total sugar and reducing sugar was noticed as Magnolia rooting started, and this reduction was parti cularly outstanding with auxin treatment. Starch content also was decreased in the later stage of cutting with auxin treatment, and was rather increased without auxin. Although sugar content soon increased as cutting started with auxin treatment in the case of Cestrum, it became reduced after rooting took place. 5) Total phenol content increased with rooting, and this was especially true when rooting started. This increase was reversed somehow regardless of auxin treatment. A decrease in phenol of Magnolia was found more striking with auxin than without in the later stage of the cutting period. 6)Avena coleoptile test for auxin-like substances presented the physiologically active factor is more in easy-to-root Magnolia liliflora than hard-to-root Magnolia kobus, and the activity of auxin-like substances was much increased with auxin treatment. The increase in the growth promoting substances was markedly pronounced when rooting just started. The active growth substances decreased in the later stage of cutting, and certain inhibitory substances started appearing. Cestrum also showed physiologically similar growth promoting substances accompanying auxin-like active substances if auxin is treated, and some strong inhibitory substances seemed to appear in the later stage of cutting. 7) Mung-bean-rooting test indicated biologically that endogenous growth substances in Magnolia all promoted mung-bean rooting, and activity of the growth substances apparently stimulated mung-bean rooting with auxin more than without. Here auxin treatment seemed to give a rise to an increased activity of endogenous growth substances in cuttings. This activity was found much greater with either NAA or IBA than ethychlozate, and showed its peak of the activity when rooting first started taking place. Certain inhibitory substances for Avena coleoptile growth strongly promoted mung-bean rooting, and it was also much like in the case of Cestrum.

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길항균주를 이용한 고추탄저병균(Colletotrichum acutatum) 방제 및 식물생장촉진효과 (Control of Colletotrichum acutatum and Plant Growth Promotion of Pepper by Antagonistic Microorganisms)

  • 한준희;김문종;김경수
    • 한국균학회지
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    • 제43권4호
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    • pp.253-259
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    • 2015
  • 고추 탄저병은 국내 고추재배에 가장 큰 피해를 일으키며, Colletotrichum acutatum이 주요 원인균이다. 본 연구에서는 포장에서 고추탄저병의 방제와 식물생장촉진효과를 선발된 길항미생물을 이용하여 평가하였다. 4개의 길항미생물은 이전 연구를 통하여 고추포장(GJ01, GJ11)과 갯벌(LB01, LB14)에서 선발하였다. 4개의 길항미생물은 대조균주 EXTN-1을 포함하여 C. acutatum과 대치배양에서 길항효과를 보였다. 식물생장촉진효과를 알아보기 위해 고추종자의 발아율과 초기생장효과, 그리고 포장에서 식물의 생장효과를 검정하였다. 그 결과 4개의 선발균주는 모두 식물생장효과가 있었다. 그 중에서도 GJ01은 초기생육에서 가장 높은 생장효과를 보였으며, GJ11은 포장에서 가장 높은 고추수확량을 얻었다. 그리고 포장에서 탄저병의 방제효과는 4개의 길항미생물 처리에 의해 63.2~72.5%의 방제가를 보였다. 현재 연구를 토대로 4개의 길항미생물은 고추 탄저병에 대한 잠재적인 생물학적 방제제로서의 가능성을 보여주었다.

Effect of Trichokonins on the Growth Characteristics of Paeonia ostii 'Fengdan' Seedling Roots

  • Chu, Peng-fei;Cao, Xing;Yang, Zhen-jing;Zhang, Xiu-sheng;Piao, Yong-ji;Jo, Hyun-Ju
    • 한국환경과학회지
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    • 제25권8호
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    • pp.1051-1056
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    • 2016
  • The growth characteristics of Paeonia ostii 'Fengdan' seedlings roots in response to trichokonins-spray treatment were investigated in this study. One-year-old seedlings of P. ostii 'Fengdan' were potted in plastic cups containing garden mold and grown under field conditions. The results showed that application of trichokonins significantly promoted root growth in P. ostii 'Fengdan' seedlings. The total root projection area, total root surface area, total root volume, total root length, root number and root diameter of seedlings treated with 0.25 mg/L trichokonins were higher by 141.70, 116.59, 119.44, 55.97, 348.88 and 127.78%, respectively, than that of the control. Thus, the results supported the hypothesis that good growth condition for roots could directly improve their nutrient absorption and utilization efficiency, promoting plant growth and development.

Rapid Detection of Cadmium-Resistant Plant Growth Promotory Rhizobacteria: A Perspective of ELISA and QCM-Based Immunosensor

  • Agrawal, Ruchi;Satlewal, Alok;Chaudhary, Manav;Verma, Amit;Singh, Rachna;Verma, A.K.;Kumar, Rajesh;Singh, K.P.
    • Journal of Microbiology and Biotechnology
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    • 제22권6호
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    • pp.849-855
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    • 2012
  • Plant growth-promoting rhizobacteria (PGPR) pseudomonads have a large number of lipopolysaccharides on the cell surface, which induces immune responses. Cd-resistant PGPR prevalent at the Cd-affected sites under biophytostabilization was monitored. Transmissiom electron microscopy was used to the study the behavior of tolerance of PGPR to cadmium level and its effect on pseudomonad strains (Z9, S2, KNP2, CRPF, and NBRI). An immunosensor was developed by immobilizing antibody (anti-Z9 or anti-S2) against selected PGPR on a piezoelectric quartz crystal microbalance (QCM). Immunosensors were found to supplement the inherent specificity of antigen-antibody reactions with the high sensitivity of a physical transducer. On comparison of the efficiency of detection with ELISA, the spectrophotometric technique, the developed immunosensor was found to be more sensitive, fast, and reliable even after regeneration for several times. Thus, the immunosensor may be used for future detection of PGPR strains after automation of the screening process.