• 제목/요약/키워드: plant enzymes

검색결과 683건 처리시간 0.028초

벼의 생육시기에 따른 일부 광호흡효소의 활성변화 (Activities of Catalase, Glycolate Oxidase, Hydroxypyruvate and NADPH-Glyoxylate Reductases at Different Growing Stages in the Leaves of Rice Plants)

  • 권영명
    • Journal of Plant Biology
    • /
    • 제22권3호
    • /
    • pp.81-84
    • /
    • 1979
  • Catalase, glycolate oxidase, hydroxypyruvate and NADPH-glyoxylate reductases activities in cell free extracts from leaves of 3 cultivars, Suwon 264, IR 36 and Jin Heung of rice plants were studied at different growing stages. Catalase and glycolate oxidase shows inclining activities toward the maximum vegetative growth whereas declining activities in either the enzymes were noticed during the maturing stage. After the photoperiodic condition exhibit increasing hydroxypyruvate and NADPH-glyoxylate reductases activities with time until maturing stage. No significant differences were found in the enzyme activities, and in analytical data of nitrogen, chlorophyll contents, dry weight and soluble proteins among the 3 cultivars.

  • PDF

Cucurbita pepo에서 분리한 Light Membrane Vesicle의 ATPase와 Phosphatase의 정제 및 특성 (Purification and Characterization of ATPase and Phosphatase of Light Membrane Vesicles Isolated from Cucurbita pepo)

  • 오승은
    • Journal of Plant Biology
    • /
    • 제33권4호
    • /
    • pp.325-332
    • /
    • 1990
  • Light membrane vesicles were isolated from the zucchini hypocotyl by floatation on ficoll density gradients and the proteins were solubilized with Triton X100. Three ATP-hydrolyzing enzymes were partially purified by ion-exchange and gel filtration chromatography and isoelectric focusing. There are plasma membrane-type ATPase whose activity was inhibited by vanadate but not by nitrate, tonoplast-type ATPase which was sensitive to nitrate but insensitive to vanadate and one having a phosphatase activity with a pI value different from that of an acid phosphatase. A fraction was obtained after DEAE-ion-exchange chromatography crossreacting with polyclonal antibodies against Ca2+ -ATPase from human erythrocytes.

  • PDF

Regulation of nicotine biosynthesis in tobacco

  • Hashimoto, Takashi
    • 한국식물생명공학회:학술대회논문집
    • /
    • 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
    • /
    • pp.22-26
    • /
    • 2005
  • Nicotine is most familiar to us as a principal pharmacologically active component of cigarettes. This alkaloid is synthesized in the root in response to insect damage and then transported to the aerial parts of tobacco plants. Here I overview enzymes and genes involved in nicotine biosynthesis, and regulatory mechanisms of gene expression involving the NIC regulatory loci and jasmonic acid.

  • PDF

Physiological and Biochemical Changes in Lucerne (Medicago sativa) Plants Infected with 'Candidatus Phytoplasma australasia'-Related Strain (16SrII-D Subgroup)

  • Ayvaci, Humeyra;Guldur, M. Ertugrul;Dikilitas, Murat
    • The Plant Pathology Journal
    • /
    • 제38권2호
    • /
    • pp.146-158
    • /
    • 2022
  • Changes in physiological and biochemical patterns in lucerne plants caused by the presence of 'Candidatus Phytoplasma australasia', which is one of the significant pathogens causing yield losses in lucerne plants, were investigated. Significant differences were evident in total chlorophyll, chlorophyll a, chlorophyll b, and protein amounts between 'Ca. Phytoplasma australasia'-positive and negative lucerne plants. Stress-related metabolites such as phenol, malondialdehyde, and proline accumulations in 'Ca. Phytoplasma australasia'-positive plants were remarkably higher than those of phytoplasma-negative plants. As a response to disease attack, phytoplasma-positive plants exhibited higher antioxidant enzymes (peroxidase and catalase) and nonenzymatic metabolite responses such as jasmonic and salicylic acids. We state that partial disease responses were revealed for the first time to breed resistant lucerne lines infected by 'Ca. Phytoplasma australasia'.

미나리 줄기(Oenanthe javanica), 율무(Coicis lachryma-jobi L. var.), 차전자(Plantaginis asiatica L.) 물 추출물이 지질대사 (Effects of Oenanthe javanica, Coicis lachryma-jobi L. var., and Plantaginis asiatica L. Water Extracts on Activities of Key Enzymes on Lipid Metabolism)

  • 이현주;정미자;김대중;최면
    • 한국식품영양과학회지
    • /
    • 제38권11호
    • /
    • pp.1516-1521
    • /
    • 2009
  • 본 연구는 미나리(Oenanthe javanica, MNR), 율무(Coicis lachryma-jobi L. var., YM) 및 차전자(Plantaginis asiatica L, CJJ) 물 추출물이 지질 대사 관련 효소들인 lipoprotein lipase(LPL), acyl-CoA synthetase(ACS) 그리고 carnitine acetyltransferase(CAT) 활성에 유익한 영향력을 미치는지 알아보았다. LPL와 ACS는 비만 모델군 Zucker fatty 흰쥐(fa/fa)와 대조군인 Zucker lean 흰쥐(lean)의 부고환 지질과 간에서 각각 분리하였다. MNR이나 YM 물 추출물 처리는 일반(lean) 및 비만(fa/fa) 흰쥐로부터 분리한 LPL 활성을 현저하게 감소시켰다. MNR, YM 그리고 CJJ 물 추출물 10000 ppm을 처리했을 때 fa/fa LPL 활성이 각각 32.5%, 30.1% 그리고 22.8% 증가하였다. Lean ACS 활성이 대조군과 비교하여 YM 물 추출물에서 현저히 증가하였고 MNR 물 추출물 처리는 대조군과 비교하여 fa/fa ACS 활성을 현저하게 증가시켰다(p<0.05). 10000 ppm MNR 물 추출물은 대조군과 비교하여 fa/fa ACS 활성을 12배까지 증가시켰다. CAT 활성이 대조군보다 10000 ppm과 2000 ppm CJJ 물 추출물 군에서 현저히 높았다. 따라서 MNR, YM 그리고 CJJ 물 추출물은 비만한 사람에게 지질 대사와 관련된 효소 활성에 기인하여 유익한 효과가 있을 것으로 추정된다.