• Journal of Crop Science and Biotechnology
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• v.11 no.1
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• pp.63-68
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• 2008

The induced genetic divergence was estimated in 44 mutant lines of finger millet variety GPU 26, developed by single and combination treatments with gamma rays, EMS and NG using three multivariate analyses. The mutant lines were grouped into eight genetically diverse clusters by multivariate D2 and canonical analyses and 11 clusters by dendrogram grouping through Gower's similarity coefficient. The clustering pattern in these three methods was almost similar. Twelve mutant lines in D2 and 13 in the dendrogram grouping method were grouped in the parental cluster(Cluster I) indicating that they did not possess enough divergence from the parent to be classified as micromutant lines. However a large proportion of mutant lines showed divergence from the parent variety and also among themselves. No definite relationship of mutagenic origin and clustering of mutant lines were observed. The mutant lines developed from the same mutagenic treatments often grouped into different clusters indicating that each mutagenic treatment was effective in inducing diverse types of changes in the nine traits studied. The hybridization program between the divergent mutant lines GE 2-2 or GE 3-4 with GG 3-1 is expected to give promising and desirable segregants in subsequent generations. Traits such as days to 50% flowering and days to maturity had major contributions to the induced genetic divergence.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.400-407
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• 2010

Zoysiagrass (Zoysia japonica Steud.), also called Korean or Japanese lawngrass, is the most popular warm-season turfgrass in Korea and is widely used for home lawns, parks, roadsides, golf courses and athletic fields. Its use is rapidly expanding in Korea and the other countries, due to its excellent characteristics which include tolerance to heat, drought and salinity. As the utilization area of this turfgrass increases, there is an increase in the demand for improved cultivars with disease and insect tolerance or with herbicide-tolerance or with extended greening periods. Conventional breeding methods have been used to improve the traits described above with limited success. However, with the advances in biotechnology, genetic transformation can be utilized for turfgrass improvement. In this paper, we review recent progress in biotechnological improvement of zoysiagrass and discuss future molecular breeding of this species.

• Journal of Plant Biotechnology
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• v.33 no.3
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• pp.209-222
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• 2006

The whole genome sequence was completed in arabidopsis and rice. Large amounts of EST data have been available from many other plants. Also, vast quantities of diverse biological data have been generated by various '-omics' technologies such as transcriptomics, proteomics, and metabolomics. Bioinformatics plays an essential role in extracting useful information from these tremendous amounts of biological data. In this review we introduced experimental methods to generate massive data, applications to plant science such as plant disease resistance and molecular breeding and bioinformatics tools and web sites available in plant biotechnology R&D. We concluded that new experimental methods and bioinfomation analysis techniques have made major contributions to the development of plant biotechnology and that bioinformatics has become a critical factor in plant biotechnology R&D.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.293-297
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• 2011

This experiment was conducted to elucidate the effects of mulching materials and removing time of the transparent polyethylene (PE) film on the growth of garlic at Uiseong experimental field, Korea. The experimental mulching materials comprised of transparent polyethylene film (0.025 mm) and net polyethylene (NPE). Plant height and leaf number of garlic were highest at PE treatment when the PE removing date was March 18 and this treatment also promoted the no. of cloves. Length of leaf sheath and bolting rate were highest and bulb weight loss rate was lowest at PE + NPE treatment when the PE removing date was March 18. But clove number was the lowest in this treatment compared to conventional PE film treatment. Conventional mulching method accelerated secondary growth rate but bulb weight loss was vice versa. There were statistically no differences in bulb diameter among treatments but conventional treatment positively focused on bulb diameter. Whenever PE film remove can suppress weeds compared to no mulching treatment but the dry weight of weeds were increasing trends as the removal dates of PE film were delayed. Transparent PE or PE + NPE treatments can be recommended to grow best quality garlic when PE film removing date is March 18.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.1
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• pp.124-132
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• 2023

This study aimed to cross between Korean and Japanese pure lines of Neopyropia strains to establish cross breeding technology and identify a superior variety that harbors the strength of both parents. Four crossing combinations were tried using three methods, resulting in 1,476 single conchocelis colonies. The three co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) markers (EF-1α/Mse I, TOP2/Mse I, car A/ApaL I) were used to distinguish heterozygotic sporophytes and their maternal lines obtained from the inter and intraspecific cross-fertilization within the wild type of Neopyropia strains. Of the 1,476 colonies, 26.9% (218) were heterozygotes obtained from the nuclear CAPS markers. Their maternal line was clearly confirmed using organelle CAPS marker and chimeric thallus was obtained from crossing experiment of Japanese N. yezoensis (♀) and Korean N. yezoensis (♂). The use of CAPS markers improved the efficiency of crossbreeding by quickly screening heterozygotes and maternal lines in the conchocelis phase, which otherwise required pigmentation mutants as genetic markers.

• Korean Journal of Plant Resources
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• v.20 no.1
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• pp.73-78
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• 2007

Efficient pollination needs abundant fertile pollen in rose breeding. This study was performed to find out efficient staining methods for the detection of fertile pollen. Aceto-carmine and Alexander's stain gave similar results in terms of percentage of normal pollen. Fluorochromatic reaction (FCR) showed the lowest normal pollen percentage because FCR stained only fertile pollen while others stained cytoplasm. Toluidine blue O (TB) showed similar percentage of normal pollen to Aceto-carmine and Alexander's, but could not clearly distinguish the clustered abnormal pollens. Alexander's stain was easy and simple, but difficult to distinguish fertile and infertile pollen. FCR showed only fertile pollen. Alexander's stain showed approximate fertility and FCR showed exact pollen fertility.

• The Plant Pathology Journal
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• v.28 no.2
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• pp.212-215
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• 2012

Pyrenophora graminea, the causal agent of leaf stripe disease, is an economically important pathogen of barley found worldwide. It is critical to clearly define and standardize the leaf stripe assessment methods to avoid subjectivity and variability between assessors. Therefore, in this study, a comparison between the proportion of diseased plants (DP) and the proportion of diseased leaves (DL) per plant was investigated. Disease assessments were made visually at multiple sample sites in artificially and naturally inoculated research and production fields during four growing seasons. There were significant differences (P = 0.001) among cultivars in mean DP and DL averages, which are consistently higher in susceptible barley. However, DP values increased linearly as DL increased. The slopes and intercepts of the DP-DL relationship were consistent over the four growing seasons. This result might make a significant contribution for leaf stripe assessment in barley breeding programme.

• Journal of the Korean Society of Tobacco Science
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• v.21 no.1
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• pp.82-88
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• 1999

The present study was conducted to compare the relative efficiency of two different haploid breeding methods in tobacco varietal development. A single FI hybrid plant from cross of two flue-cured cultivars of Nicotiana tabacum L., Bright Yellow4(BY4) and NC95, was used to develop the 30 anther derived dihaploid (ADH) lines and maternally-derived dihaploid (MDH) lines utilizing Nicotiana africana, respectively. As compared with mid-parent, mean of ADH lines showed increase in number of leaves, delay in days to flower and narrow in width of leaf. However, no significant differences in the other investigated characters were recognized. MDH lines also showed narrow width of leaf, while no significant differences in the other characters were observed. The variations of the investigated characters were generally greater in ADH than MDH lines. MDH lines had higher height of plant and shorter days to flower than ADH lines, while the other characters did not show remarkable differences. The degree of heritability for each of the characters observed between ADH and MDH was almost the same. The characters showing high heritability value were height of plant, number of leaves, days to flower, and yield, while those showing relatively low value were length of leaf, width of leaf, and total alkaloid content. Predicted gains from selection for increased yield were calculated for both populations(ADH, MDH) and correlated responses associated with selection for yield were estimated. Height of plant, width of leaf, days to flower, and concentration of reduceing sugar would be expected to improve with selection for yield much faster in the MDH population than in the ADH.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.345-352
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• 2009

Zoysiagrass (Zoysia japonica Steud) is a warm season turfgrass species widely used for sports field and golf courses. Many cultivars are propagated through vegetative methods. This study was conducted to develop an optimum culture medium and culture conditions for embryogenic callus induction and plant regeneration, and to establish a cell suspension culture system for use in zoysiagrass breeding and propagation. The results indicated that adding $Cu^{++}$ at 2.5 mg $L^{-1}$ to the induction medium was optimum for callus induction. Increasing the numbers of sub-culture cycles improved the quality of calli. The optimum dosage for cell suspension culture ranged from 2.5 to 10 mL. The embryogenic callus suspension used in this study had a plant regeneration rate of 58%.

• Applied Biological Chemistry
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• v.14 no.3
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• pp.221-227
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• 1971

Potential grain yield of rice plant was calculated from potential grain yield of each leaf for two lines of IR 667 and two commercial varieties (Jinhung and Paldal). According to the percentage contribution of each leaf the pattern of potential grain yield of IR 667 lines could be grouped into the upper leaf-dependent type indicating fast senescence while that of commercial varieties could be grouped into the lower leaf-dependent type indicating shade resistance. The comparisons between potential grain yields and apparent grain yields indicate that the commercial varieties having a comparably unfavorable plant type thus remained much behind the potential yield. The significance of potential grain yield was discussed in relation to nutrio-physiology and breeding. Methods for potential yield determination were also discussed.