• 한국균학회지
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• 제14권1호
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• pp.89-91
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• 1986

Sixteen seed samples of nine vegetable crops imported from Japan were tested for plant quarantine by routine methods of seed health testing. Nineteen species of fungi including Alternara alternata were identified from the seed samples. Some species were very frequently detected in the seeds. In this experiment, Verticillium albo-atrum, V. dahliae and Macrophomina sp. from the seeds of spinach, and Cercospora capsici, Drechslera hawaiiensis and V. albo-atrum from the seeds of sweet pepper were detected for the first time, respectively. It has been reported that they had a pathogenicity on some vegetable crops in Japan and other countries. The data suggest that an epidemic damage may be occurred if the infected seeds are sown and cultivated in Korea.

• 한국작물학회지
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• 제53권spc호
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• pp.78-83
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• 2008

콩에 함유된 성분 중 가장 많은 양으로 존재하며, 인축의 주요 단백질 공급원으로 이용되고 있는 콩 단백질의 함량을 1립 비파괴 근적외 분광분석법을 이용하여 동일품종의 개체 내에서 콩이 달린 착협 위치에 따라, 또 콩에서 단백질 함량을 분석하기 위해 개체 내에서 분석시료를 취할 때 개체를 대표할 수 있는 최소 양을 실험적으로 검토한 결과를 요약하면 다음과 같다. 1. 선녹콩 개체 내 단백질 함량은 정규분포를 보였으며, 개체 간에 단백질 함량의 차이가 인정되었다. 2. 콩의 착협 위치에 따른 단백질 함량은 지표면에 가까울수록 단백질 함량이 높았고, 콩 식물체의 윗부분으로 갈수록 단백질 함량이 낮아지는 경향을 나타내었다. 3. 콩에 함유된 단백질의 함량을 정량적으로 분석하기 위해 필요한 최소 시료량을 추정한 결과 최소 10립 이상 혹은 개체 내 총 종자량의 20% 이상을 취하면 개체를 대표하는 단백질 함량을 얻을 수 있는 것으로 조사되었다.

• 한국자원식물학회지
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• 제32권5호
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• pp.457-462
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• 2019

본 연구는 각시괴불나무 종자의 휴면유형과 발아특성을 구명하기 위하여 수행되었다. 수분흡수 실험결과, 각시괴불나무 종자는 수분흡수가 원활히 이루어졌다. 따라서, 물리적 휴면은 가지지 않는 것으로 판단된다. 각시괴불나무 종자는 탈리 시점에 미숙배를 가지고 있으며, 발아직전까지 배/종자 비율이 약 46% 증가하였다. 온도실험결과, $5^{\circ}C$에서는 발아가 전혀 이루어지지 않았다. $20^{\circ}C$에서는 파종 후 1주부터 발아가 시작되었으며 최종 발아율은 2주차에 86.7%였다. $15^{\circ}C$에서는 파종 후 2주부터 발아를 시작하였으며, 최종 발아율은 4주차에 75.0%였다. $25^{\circ}C$에서 파종 후 1주부터 발아가 시작되었으며 최종 발아율은 19주차에 48.3%였다. 결론적으로, 각시괴불나무 종자의 발아를 위한 최적온도는 가장 높은 최종 발아율과 가장 짧은 평균 발아 일수를 나타낸 $20^{\circ}C$이다. 각시괴불나무 종자는 $5^{\circ}C$를 제외한 모든 온도에서 4주 이내에 발아하였기 때문에 생리적 휴면은 관찰되지 않았다. 따라서, 각시괴불나무 종자는 오직 형태적 휴면만을 가지는 것으로 판단되었다.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.255-263
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• 2021

Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl₂ (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 ℃; thus, they could be useful for transfer and exchange of P. foetida germplasm.

• Journal of Ecology and Environment
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• 제43권2호
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• pp.254-262
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• 2019

Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15-25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50-80% of the length of the seeds' development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L. insularis and L. harae germinated within 4 weeks after sowing at 15 ℃ and 20 ℃, respectively. Contrarily, L. praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 ℃, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 ℃, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 ℃). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 ℃) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 ℃, 20 ℃, 15 ℃, and 20 ℃, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.

• Plant Resources
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• 제5권1호
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• pp.1-6
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• 2002

Genetic improvement of the cultivated soybean [Glycine max (L.) Merr] may be possible through hybridization with its wild progenitor, G. soja Sieb. & Zucc. Interspecific cross between G. max (Hwangkeumkong) and G. soja (IT.182932) was made in the summer of 1997. In F$_2$ the percentage of plant height, nodes per plant, and pods per plant were high but gradually reduced from F$_2$ to F$_4$. In contrast pod length, seeds per pod, and 100-seeds weight were increased gradually through generations advanced. Wild variation as evident in F$_2$ in plant height, number of branches, pods per plant, and 100-seeds weight. Twenty six percent of the F$_2$, 44 % of the F$_3$ and 60% of the F$_4$ segregants showed more G. max traits. The combination of useful traits from both species is possible through interspecific hybridization. The characters that could be transferred from wild species to cultivated species are more pod number, better capacity, and resistance to disease and insects. The interspecific derivatives offer scope for selection for high grain yield. Therefore, introducing genes from G. soja to G. max could be contribute to greater genetic diversity of future cultivars. And semicultivated soybean had some desired characteristics including tolerance to adverse environments and multi-seed characters. It means the infusing of semicultivated germplasm to the cultivated soybean could increase number of seeds and pods per plant significantly, and consequently could enhance selecting potential on yield.

• 식물조직배양학회지
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• 제27권4호
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• pp.283-297
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• 2000

Seeds are an ideal repository for recombinant proteins in molecular farming applications. However, in order to use plant seeds efficiently for the production of such proteins, it is necessary to understand a number of fundamental biological properties of seeds. This includes a full understanding of promoters which function in a seed-specific manner, the subcellular targeting of the desired polypeptide and the final form in which a protein is stored. Once a biologically active protein has been deposited in a seed, it is also critical that the protein can be extracted and purified efficiently. In this review, these issues are examined critically to provide a number of approaches which may be adopted for production of recombinant proteins in plants. Particular attention is paid to the relationship between subcellular localization and protein extraction and purification. The robustness and flexibility of seed-based production is illustrated by examples close to or already in commercial production.

• Journal of Plant Biology
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• 제18권4호
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• pp.155-160
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• 1975

Growth responses of lettuce seeds germinated in aqueous solutions of $SO_2$ were investigated for mode of action studies of this gas. The concentration and pH of $SO_2$ solution greatly affected the growth of germinating seeds. Root growth was more sensitive to $SO_2$ than hypocotyl growth. The sensitivity of total seedling growth lay between root and hypocotyl growth. Growth inhibition of germinating seeds appeared more serioius when $SO_2$ was added during the early stage of germination.

• 식물병연구
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• 제27권1호
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• pp.8-16
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• 2021

본 연구에서는 마이크로웨이브 플라즈마(microwave plasma)를 이용하여 종자에 감염된 Acidovorax citrulli 11-251의 살균효과를 확인하였다. 감압처리를 통하여 수박 종자에 A. citrulli 11-251를 인공접종하여 이병 종자를 제작하였으며, Aac ImmunoStrip및 scanning electron microscope 확인 결과, 종피 및 종자 내부에 1×107/30분 처리한 조건에서 감염되었음을 확인하였다. 플라즈마 처리는 50 W (3분, 5분, 10분), 80 W (3분, 5분, 10분), 100 W (3분, 5분, 10분) 조건으로 실험 결과, 인공감염종자를 플라즈마 처리 시 대조구와 비교해보면 종피의 경우 80W/10분, 99.56%의 살균 효과를 보였고, 종자 내부는 80 W, 100W/10분, 100%의 높은 살균 효과를 보였다. 인공접종 종자를 사용하여 플라즈마 살균장치의 종자살균 효과를 확인하였다. 하지만 발아율은 무처리구보다 플라즈마 처리 시 급격히 떨어지는 것을 확인하였다. 주사전자현미경 관찰 결과, 플라즈마 처리가 A. citrulli 11-251의 세포막을 파괴하는 것을 확인하였다. 또한, A. citrulli가 접종된 수박 종자를 이용하여 플라즈마 처리 시 살균 및 발아율을 높일 수 있는 최적 조건에 대한 추가 실험이 수행될 예정이다.

• 한국자원식물학회지
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• 제6권2호
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• pp.135-140
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• 1993

This study was attempted to establish the propagation and cultivation methods of Rhodiola elongata Fisch. et Mey. as a part of development of northern plant resources. Seeds were treated and investingated at the 15, 20, 25$^{\circ}C$ and 100, 200, 400, 800ppm of the growth regulator $GA_3$, BA, and Kinetin. The results obtained are summarized as follows: Seeds of R. elongata were the most vigorously germinated at 15-20$^{\circ}C$, and rate of germination at 25$^{\circ}C$ decreased gradually. Germination rate of seeds that treated growth regulators was remarkably increased than non-treated ones, and $GA_3$ treatment among the growth regulators showed the best effects. The effective concentration of $GA_3$ for germination of R. elongata seeds was 400 ppm.