• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.535-539
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• 2009

The time of seed collection is regarded as one of major concerns to obtain sound seeds. The physical and germinal aspects of Cryptomeria japonica D. Don (Taxodiaceae) seeds were analyzed to determine the optimum harvesting time in Korea. Cones were picked every 10 days from the $30^{th}$ of July to the $30^{th}$ of October in both 2005 and 2006. Seeds were collected from picked cones. Seed size and weight were not significant in two consecutive years. The 1,000-seed weight was 3.3 g for cones picked at the $18^{th}$ of August and 5.3 g for cones picked at the $30^{th}$ of September. The size of seeds was increased as the time of collection from the $18^{th}$ of August to the $30^{th}$ of September: from 19.3 mm to 21.3 mm in length and from 15.8 mm to 18.5 mm in width. Average germination rates in 2005 was 18.3% and 19.6% in 2006. The highest germination rate was 34.3% from seeds collected at the $30^{th}$ of September in 2005. In 2006, the highest germination rate was 31.7% for seeds collected at the same date as the 2005 seeds. After the end of September, germination rate was decreased in both years. The results implied that the best cone picking time for Korean C. japonica seeds is around the end of September.

• Korean Journal of Plant Resources
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• v.22 no.2
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• pp.111-115
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• 2009

The seeds of Phellodendron amurense have difficulty to obtain because these plants show dioecism with independent distributions. This experiment was conducted to find the effects of soaking and prechilling treatment on the germination of P. amurense seeds. Seeds were soaked for 3, 5 and 10 days (soaking treatment), and placed on petri-dish at $4^{\circ}C$ for 10, 20, 30 and 50 days after soaked at room temperature for 24 hours (prechilling treatment). After the treatments, germination percentage, mean germination time, germination speed and germination performance index were analyzed. As a result of sowing, prechilled seeds for 30 days had the highest percent of germination, whereas all of the soaked seeds showed lower percent of germination than non-treatment (control). In case of mean germination time, only prechilling for 30 days was more effective than control. In germination speed, all of the soaked seeds had lower values than control whereas all prechilled seeds except prechilled seeds for 20 days showed higher values than control. Especially prechilled seeds for 30 days showed the highest rate among the prechilling treatments. Germination performance index was similar to germination speed.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.38-38
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• 2021

The seeds of rice with variable status were examined the germination rate after 10 years of long-term storage (-18℃) conservation. For seeds of wild relatives, 2 accessions were examined and germination rate of all accessions showed no changed after freezing conservation. For seeds of native germplasm, 1,259 accessions were examined and germination rate of 696 accessions was increased or showed no change after 10 years of long-term storage. Germination rate of 432 accessions was decreased with below 15% of initial germination rate. For 79 accessions of native germplasm, germination rate of seeds was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. For seeds of developed varieties, 873 accessions were examined and germination rate of 486 accessions of developed varieties was increased or showed no change after 10 years of long-term storage. Germination rate of 251 accessions was decreased with below 15% of initial germination rate. For 25 accessions of developed varieties, germination rate of seeds was decreased with above 15% of initial germination rate after 10 years of long-term storage, which is needed to be rejuvenated. For seeds of developed line, 2,131 accessions were examined and germination rate of 1,245 accessions of developed line was increased or showed no change after 10 years of long-term storage. Germination rate of 588 accessions was decreased with below 15% of initial germination rate. For 96 accessions, germination rate of seeds was decreased with above 15% of initial germination rate, which is needed to be rejuvenated.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.557-563
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• 2016

To improve the germination of Spiraea fritschiana seeds for mass propagation, we evaluated the effect of a range of temperatures, cold stratification periods, and gibberellic acid ($GA_3$) treatments on three germination characteristics. Final germination percentage (FGP) increased as the temperature for seed germination increased, up to $30^{\circ}C$, while the mean germination time (MGT) and the mean number of days to 30% germination ($T_{30}$) decreased when seeds were germinated at $25-30^{\circ}C$. The optimum germination temperature of S. fritschiana seeds is approximately $30^{\circ}C$ considering FGP, MGT, and $T_{30}$ together. FGP increased with the duration of cold stratification up to a period of 6 weeks, but declined after 8 weeks of cold stratification, as prolonged cold stratification can induce dormancy with a resultant decline in germination. Pretreatment with 6-8 weeks of cold stratification or soaking seeds in distilled water or $500mg{\cdot}L^{-1}$ $GA_3$ for 24 h accelerated and increased the germination of S. fritschiana seeds, regardless of temperature. However, further study might be required to evaluate the effect of $GA_3$ concentrations lower than $500mg{\cdot}L^{-1}$ on the promotion of germination in S. fritschiana seeds.

• Korean journal of applied entomology
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• v.21 no.1 s.50
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• pp.23-26
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• 1982

The present study was carried out to investigate the change of peroxidase activity of soybean seed infected with Cerrospora kikurhii. The protein content, polyphenol oxidase activity and peroxidase isozyme pattern in health and infected soybean seed were also compared. 1. The peroxidase activity was substantially higher in the infected soybean seeds than that in the healthy seeds either cracked or not. No significant differences in protein content were recognized among the seeds tested. 2. No significant differences in peroxidase activities and protein contents were notified between healthy and infected seeds from the measurements on each parts of dissected seeds, cotyledon and seedcoat, however the peroxidase activity in the seed coat of the stained seed was 2.5 times to healthy seed. 3. The activities of polyphenel oxidase were undectable in both healthy and diseased seeds. 4. The electrophoretic Patterns of the Peroridase isozyme were the same between healthy and in footed seed. 5. Therefore, the increase of peroxidase activity in infected soybean seedcoat was mainly due to the biochemical reaction against the pathogen.

• Research in Plant Disease
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• v.13 no.3
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• pp.145-151
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• 2007

Xanthomonas axonopodis pv. glycines is the causal agent of bacterial pustule of soybean(Glycine max. (L.) Merr), which is one of the most prevalent bacterial diseases in Korea. In this study, Polymerase Chain Reaction (PCR) assay was applied to detect Xanthomonas axonopodis pv. glycines and to survey on seed contamination in 36 soybean cultivars of Korea. And we have to compare PCR assay with dilution-plating assay of detection and identification. We confirmed detection of pathogen from artificial infected seeds and natural Infected seeds using PCR assay. This assay gave results similar to a seed-wash dilution plating assay and proved more effective than classical methods. Results of survey on seed contamination by X. axonopodis pv. glycines from 36 cultivar seeds showed that the pathogen was detected from Pungsan-namulkong, Mallikong, Taekwangkong, Daemangkong, Ajukkarikong using PCR assay. Therefore, The PCR assay provides a sensitive, rapid tool for the specific detection of X. axonopodis pv. glycines in soybean seeds.

• Weed & Turfgrass Science
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• v.5 no.1
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• pp.10-16
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• 2016

Proper actions such as discard, return or manufacturing should be taken to imported plants contaminated with quarantine weeds. Heat-treatment has been used as a processing method in many countries such as U.S., Australia, Canada as well as Korea. This study was carried to find appropriate heat-treatment methods for devitalizing dodder seeds mixed with plant seeds. Two species of dodder seeds and six imported seeds were treated for 1, 5, 10, 15, 30, 45 minutes or 1, 2, 4, 5, 6, 8 hours at the temperatures of $75^{\circ}C$, $80^{\circ}C$, $85^{\circ}C$, $90^{\circ}C$, $95^{\circ}C$, $100^{\circ}C$ and $120^{\circ}C$. According to the study, dodder seeds were devitalized at the conditions of $85^{\circ}C$ for 2 hours, $90^{\circ}C$ for 30 minutes, $95^{\circ}C$ and $100^{\circ}C$ for 15 minutes, $110^{\circ}C$ for 10 minutes and $120^{\circ}C$ for 5 minutes. Meanwhile, seed vigours of Cosmos bipinnatus and Callistephus chinensis had no difference when compared with untreated control at the heat-treatment conditions of $85^{\circ}C$ for 2 hours, $90^{\circ}C$ for 30 minutes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.2
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• pp.134-138
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• 2000

Most parts of alfalfa plant have been reported to contain autotoxic substances that inhibit seed germination and early seedling growth, however, the chemical(s) is not still studied much. Effect of seed leachates of 'Vernal' alfalfa (Medicago sativa L.) was evaluated for inhibition of alfalfa germination and root growth through bioassay. Alfalfa seeds were extracted in 1 L deionized water for 1 h after soaking and the leachates caused to reduce root length of alfalfa significantly as the soaking time increased. Crude seeds at 4 g L$L^{-1}$ exudated autotoxins that reduce significantly root length by 34 % compared to the control, when the seeds soaked in deionized water for 24 h. However, the extracts did not affect final germination as well as speed of germination. Extracts from ground seeds significantly reduced speed of germination (GT 50) and root length. The results indicate that release of autotoxic substances from seeds during seed imbibition was increased with increase of soaking time and seed amount, and that autotoxicity was more occurred in ground seeds than in crude seeds.

• Current Research on Agriculture and Life Sciences
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• v.22
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• pp.1-12
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• 2004

Pod-edible bean or snap bean is a fairly new crop to domestic farmers but the national demand is steadily increasing in recent years along with the development of western food business and change in dietary patterns. At the same time, much efforts are being made to export it to foreign country, mainly to Japan. The amount of seeds introduced from outside is also continuously increasing along with the enlargement of area planted for the crop. Hybridization breeding for the crop has already been started to supply the cheaper and better seeds which will reduce the seed costs and foster the higher income to the farmers. In this experiment, several technologies related with the production of quality seeds are preliminary investigated. Some of the results obtained are summarized as follows; 1. Highly significant interaction was recognized between planting dates and no. of pods per plant and no. of branches but no interaction between planting dates and plant height and no. of nodes on main stem. Days to maturity was proportionally reduced to later planting dates. 2. Rate of viviparous pods and seeds was gradually increased in later planting dates but rate of germination was increased in earlier planting dates with lower germination rate in white seed coat grains than in colored seed ones. 3. Seed yield was higher in the earlier planting dates with a great deal of varietal difference. Early to mid April was considered to he the optimum planting dates for snap bean in Kyungbuk area. High correlation was recognized between seed yield and no. of pods per plant, no. of seeds per plant, and 100 seed weight. 4. Days to flowering was three and seven days longer in Cheongsong, high mountainous area than in Kunwi, somewhat prairie lowland. One hundred seed weight was also higher in Cheongsong than in Kunwi. Rate of viviparous grains, pods, and decayed seeds was higher in Cheongsong but, at the same time, the rate of germination and seed yield was also higher in Cheongsong. 5. One hundred seed weight of KLG5007 increased continuously up to 35days after flowering and decreased thereafter but that of KLG50027 increased to 40days after flowering and slowly reduced thereafter. The content of crude oil reached to maximum at 40 days after flowering and reduced thereafter. The rate of germination in Gangnangkong 1 was the highest, 89.3%, at 35 days after flowering and reduced thereafter while that in KLG50027 reached to maximum, 70.7%. at 40days after flowering and reduced thereafter. Thus, the optimum harvesting time for snap bean was considered to be 35~40days after flowering. 6. The snap bean pods at yellow bean stage easily became viviparous ones under saturated moisture conditions for 24 hours at $25{\sim}30^{\circ}C$. Therefore, it is recommended to harvest pods somewhat earlier than yellow-bean stage and let them do post maturing, especially when it is to be rained.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.364-369
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• 2015

Safflower (Carthamus tinctorius) seeds are wellknown traditional oriental medicines that have long been used for the remedies of blood stasis and bone formation in east Asia. In this study, ethyl acetate (EtOAc) was used for extraction of the main chemical compounds from C. tinctorius seeds. Four major compounds were identified, acacetin, cosmosiin, N-feruloyl serotonin and N-(p-coumaroyl) serotonin. Each compound was evaluated for its inhibitory activity against the inflammatory process of macrophages. All compounds significantly inhibited production of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and pro-inflammatory cytokines. The protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dramatically decreased by serotonins in a dose-dependent manner in LPS-stimulated RAW 264.7 macrophages. These results suggest that serotonin derivatives from safflower seeds may reduce inflammation-related diseases.