• Journal of fish pathology
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• v.17 no.2
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• pp.131-137
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• 2004

In the present study, phosphatidylcholine (PC) hydrolyzing enzyme had been isolated from membrane of flounder liver. PC hydrolyzing enzyme solubilized in 1% Triton X -100 from membrane was partially purified by sequential chromatography on Heparin Sepharose CL-6B and Heparin-5PW columns. The products by membrane-bound hydrolyzing enzyme were identified as phosphatidic acid and choline, but in the presence of primary alcohol, phosphatidylethanol was produced at the expense of phosphatidic acid. These data suggest that membrane-bound enzyme may be a PC-phosphoipase D (PLD) type. The enzyme had pH optimum at below 6.0 and temperature optimum at $37^\circ{C}$. The activity of PC-PLD was dose-dependently increased by $Ca^{2+}$ but not $Mg^{2+}$. The activity of PC-PLD was stimulate by PC, PIP2 and PE.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.3
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• pp.192-197
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• 1990

The study of characteristics of phosphorus release in Nakdong Estuary Barrage was conducted in June and July, 1989. Batch type reactor was designed for evaluating of release flux. The fractional composition of organic phosphorus and inorganic phosphorus in sediment were $34.7\%,\;66.7\%$, respectively. Inorganic phosphorus was fractionated into three types; Adsorbed-P, Non Apatite Inorganic-P, Apatite-P. The major forms of inorganic phosphorus were Non Apatite Inorganic-P($61.1\%$) and apatite-P($30.0\%$). Release rates of phosphorus from sediment were $-4.4mg/m^2/d$ in aerobic condition, and $39.4mg/m^2/d$ in anaerobic condition. According to the result of this study, sediment plays an important role in Nakdong Estuary Barrage as an internal load source of phosphorus.

• Polymer(Korea)
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• v.33 no.6
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• pp.575-580
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• 2009

Carbon fabric-reinforced silicon carbide composites (C/SiC) have attracted a considerable attention for high temperature structural application because of their outstanding oxidation resistance property and thermal shock resistance. In this study, we reported on the preparation of C/SiC composites by the polymer impregnation and pyrolysis (PIP) method. For this, polycarbosilane solution was impregnated into the carbon fabric and then cured by electron beam irradiation under argon atmosphere. Afterwards, the cured composite was pyrolyzed at $1300^{\circ}C$ for 1 h under argon atmosphere to produce the C/SiC composite. The porosity and density of the C/SiC composite were 13.5% and $2.44\;g/cm^3$, respectively, when the impregnation of the carbon fabric with the 30 wt% polycarbosilane solution conducted four times. In addition, in the isothermal experiment at $1500\;^{\circ}C$ in air for 5 h, the 95.9 wt% of the C/SiC composite was remained, indicating that the prepared C/SiC composite has a outstanding oxidation resistance.

• The Korean Journal of Physiology and Pharmacology
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• v.19 no.4
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• pp.357-363
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• 2015

The purpose of this study was to compare the inhibitory effect of bevacizumab on human Tenon's fibroblasts (HTFs) cultured from primary and recurrent pterygium. Cultured HTFs were exposed to 2.0, 5.0, 7.5, and 15.0 mg/mL concentration of bevacizumab for 24 hours. The 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyl tetrazolium bromide and lactate dehydrogenase leakage assays were then performed to assess fibroblast metabolism and viability. The matrix metalloproteinase (MMP), procollagen type I C terminal propeptide (PIP), and laminin immunoassays were performed to examine extracellular matrix production. Changes in cellular morphology were examined by phase-contrast and transmission electron microscopy. Both metabolic activity and viability of primary and recurrent pterygium HTFs were inhibited by bevacizumab in a dose-dependent manner, especially at concentrations greater than 7.5 mg/mL. Both types of HTFs had significant decreases in MMP-1, PIP, and laminin levels. Distinctly, the inhibitory effect of bevacizumab on MMP-1 level related with collagenase in primary pterygium HTFs was significantly higher than that of recurrent pterygium. Significant changes in cellular density and morphology both occurred at bevacizumab concentrations greater than 7.5 mg/mL. Only primary pterygium HTFs had a reduction in cellular density at a bevacizumab concentration of 5.0 mg/mL. Bevacizumab inhibits primary and recurrent pterygium HTFs in a dose-dependent manner, especially at concentrations greater than 7.5 mg/mL. As the primary HTFs produces larger amounts of MMP-1 compared to recurrent HTFs, significant reduction in MMP-1 level in primary pterygium HTFs after exposure to bevacizumab is likely to be related to the faster cellular density changes in primary pterygium HTFs.

• Journal of Life Science
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• v.28 no.5
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• pp.524-531
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• 2018

The essential oil from Abies koreana E.H. Wilson had been developed, however, its efficacy has not yet been studied especially in terms of skin care research. The aim of this study is to investigate the effects of Abies koreana extracts (AKE) on melanogenesis and wrinkle formation in B16F10 melanoma cells (B16F10) and human dermal fibroblast cell line (HDF). The essential oil was extracted by hydrodistillation method and purified by anhydrous sodium sulfate. At a concentration of $10^{-5}$-fold, viability in these cells had been defined by cytotoxicity assays. Anti-melanogenic effects on B16F10 were evaluated using tyrosinase inhibition assay, and real-time PCR for verifying gene expression of tyrosinase, tyrosinase related protein-1 and -2 (TRP-1 and -2). AKEs reduced about 5-fold of tyrosinase inhibitory activity compared to ${\alpha}$-melanocyte-stimulating hormone (${\alpha}$-MSH)-induced group and about 30% reduction compared to Arbutin induced group. The mRNA levels of three melanin-related factors were increased, separately. To investigate the effects of anti-wrinkle, procollagen type I c peptide synthesis assay (PIP) and Western blot were performed. At AKE-treated group, PIP was up-regulated and the expression of collagen type 1 and matrix metalloproteinase (MMP)-1 were improved. Furthermore, AKE presented anti-wrinkle effects by increasing UVB-inhibited collagen type 1 expression, and reducing UVB-induced MMP-1 production at $60mJ/cm^2$ of UVB radiation. Therefore, Abies koreana extracts has potentials as a safe and an effective skin ingredient for whitening and anti-wrinkle.

• Tuberculosis and Respiratory Diseases
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• v.44 no.6
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• pp.1318-1325
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• 1997

Background : Since the late 1960s, mechanical ventilation has been accomplished primarily using volume controlled ventilation(VCV). While VCV allows a set tidal volume to be guaranteed, VCV could bring about excessive airway pressures that may be lead to barotrauma in the patients with acute lung injury. With the increment of knowledge related to ventilator-induced lung injury, pressure controlled ventilation(PCV) has been frequently applied to these patients. But, PCV has a disadvantage of variable tidal volume delivery as pulmonary impedance changes. Since the concept of combining the positive attributes of VCV and PCV(dual control ventilation, DCV) was described firstly in 1992, a few DCV modes were introduced. Pressure-regulated volume control(PRVC) mode, a kind of DCV, is pressure-limited, time-cycled ventilation that uses tidal volume as a feedback control for continuously adjusting the pressure limit However, no clinical studies were published on the efficacy of PRVC until now. 'This investigation studied the efficacy of PRVC in the patients with unstable respiratory mechanics. Methods : The subjects were 8 mechanically ventilated patients(M : F=6 : 2, $56{\pm}26$ years) who showed unstable respiratory mechanics, which was defined by the coefficients of variation of peak inspiratory pressure for 15 minutes greater than 10% under VCV, or the coefficients of variation of tidal volume greater than 10% under PCV. The study was consisited of 3 modes application with VCV, PCV and PRVC for 15 minutes by random order. To obtain same tidal volume, inspiratory pressure setting was adjusted in PCV. Respiratory parameters were measured by pulmonary monitor(CP-100 pulmonary monitor, Bicore, Irvine, CA, USA). Results : 1) Mean tidal volumes($V_T$) in each mode were not different(VCV, $431{\pm}102ml$ ; PCV, $417{\pm}99ml$ ; PRVC, $414{\pm}97ml$) 2) The coefficient of variation(CV) of $V_T$ were $5.2{\pm}3.9%$ in VCV, $15.2{\pm}7.5%$ in PCV and $19.3{\pm}10.0%$ in PRVC. The CV of $V_T$ in PCV and PRVC were significantly greater than that in VCV(p<0.01). 3) Mean peak inspiratory pressure(PIP) in VCV($31.0{\pm}6.9cm$ $H_2O$) was higher than PIP in PCV($26.0{\pm}6.5cm$ $H_2O$) or PRVC($27.0{\pm}6.4cm$ $H_2O$)(p<0.05). 4) The CV of PIP were $13.9{\pm}3.7%$ in VCV, $4.9{\pm}2.6%$ in PVC and $12.2{\pm}7.0%$ in PRVC. The CV of PIP in VCV and PRVC were greater than that in PCV(p<0.01). Conclusions : Because of wide fluctuations of VT and PIP, PRVC mode did not seem to have advantages compared to VCV or PCV in the patients with unstable respiratory mechanics.

• Proceedings of the KSME Conference
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• 2007.05b
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• pp.2438-2443
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• 2007

In order to guarantee the performance of electronic products. It is needed to improve the cooling performance of heat sink. So this paper has been made to investigate the cooling performance for the aluminum heat sink using pulsating heat pipe(PHP). The pulsating heat pipe was used as a heat spreader. Working fluid was R-22. Heater (50 mm ${\times}$ 50 mm ${\times}$ 3mm) was attached to heat sink and it generated 30W, 60W, 80W, 100W. Heat sink was tested for forced convection with 1${\sim}$4m/s of inlet air velocity. And both type heat sinks were carried out by using CFD simulation. This study showed that pulsating heat pipe can be a good tool to improve cooling performance of heat sink.

• Archives of Reconstructive Microsurgery
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• v.11 no.1
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• pp.41-46
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• 2002

Purpose : To assess the clinical results of second toe-to-finger transfer in traumatic amputated index finger. Materials and Methods : For the clinical evaluation, we have analyzed 12 patients with ROM of finger joints, pinch power, static two point discrimination, life functional assessment, and patient's satisfaction. Results : In genral ROM was $54.4^{\circ}$ at MP joint, $17^{\circ}$ at PIP joint and $6.7^{\circ}$ at DIP joint. Pinch power was good in 3 cases, fair in 7 cases, and poor in 2 cases. Daily life activity and patient's acceptance were satisfactory. Conclusion : Although transfered toe function may be poorer than normal finger, the hand was restored to a useful, sensate and versatile functional unit.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.10a
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• pp.179-184
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• 1995

PIPS (Plant Information Processing System)은 발전소 입력신호를 이용하여 운전원에게 체계적인 정보를 제공하여 발전소를 안전하고 효율적으로 운전하도록 지원하는 발전소 감시 및 운전원 지원 시스템이다. UNIX 운영체제 및 workstation 상에 설계된 PIPS는 실시간 처리를 위한 kernel로 RTAP/Plus를 사용하였으며, SL-GMS를 이용하여 GUI (Graphical User Interface)를 개발하였다. 본 논문에서는 계층적 구조를 갖는 데이타베이스 관리 시스템을 이용하여 개발된 PIPS 데이타베이스의 특성 및 구조에 관하여 설명한다. 또한, point name을 이용하여 계층적 구조를 구축하는 소프트웨어를 개발하여 울진 3&4호기 설계 자료로부터 데이타베이스를 구축하였다. 이 시스템은 HP 715/100 workstation과 HP-UX 9.05 운영체제 개발환경에서 개발되었다.

• IE interfaces
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• v.16 no.4
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• pp.432-440
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• 2003

The inter-organizational business process is one of the hot issues in B2B e-commerce. In this paper, through the survey of the inter-organizational business process integration and the analysis of B2Bi related standards, e.g., BPML, WSDL, WSCI, and BPSS, we propose a method of linking BPML and BPSS specifications that can be used to integrate private processes with the public processes among enterprises. We also suggest an application example of the method by redefining a PIP of RosettaNet to the BPSS specification. The basic principle of the method is to maintain the independency of the existing BPML and BPSS applications and to construct an intermediary transformation module between the two applications.