• 한국염색가공학회지
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• 제24권3호
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• pp.196-203
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• 2012

The purpose of this study was to investigate usefulness of the pine needles extract on Atopic Dermatitis(AD)-like skin lesions. To investigate the effect of pine needles extract in vivo, atopic dermatitis (NC/Nga) mice using DNCB (2.4-Dinitrochlorbenzene) was used. NC/Nga mice were challenged with DNCB during 2 weeks to develope AD-like skin lesion. After that, pine needles extract was applied to AD-like skin lesion on the backs of the NC/Nga mice during 3 weeks. The efficacy of pine needles extract in the NC/Nga mice was evaluated by measurement of the skin lesion severity(NC mouse score), the serum IgE level, epidermal thickness changes, and mast cell number. Blood was collected from the retro-orbital area and the level of IgE in the blood was measured. The epidermal thickness and mast cell number were observed by microscopic method after H&E stain. The serum IgE levels were decreased after treatment with pine needles extract. The epidermal thickness and mast cell number were decreased after treatment with pine needles extract. To conclude, the topical application of pine needles extract suppressed the progression of AD-like skin lesion.

• 한국응용과학기술학회지
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• 제22권1호
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• pp.71-76
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• 2005

We extracted pine-needles using ethanol as solvent, and we obtained the refined oil component from pine-needles extract. Also we tested the tyrosinase activated inhibition effect with melanin experiment and analysed with ICP/OES and UV/VIS. Accordingly we obtained the next conclusion from the result of this experiment. From the first result of this experiment, we could know that the degree of recovery of refined oil component from pine-needles extract appeared in about 8.0%. From the second result of this experiment, we could know that the tyrosinase activated inhibition rate increased more and more in case of increasing concentration of pine-needles, green-tea, vitamine-C. Also we could know that vitarnine-C influences to tyrosinase activated inhibition contained in pine-needles. From the third result of this experiment, we could know that inorganic materials of Ca, Mg, V, Mn, etc contained in pine-needles detected with ICP/OES analysis, and the absorbance of pine-needles extract appeared very high in UV/VIS analysis.

• 한국응용과학기술학회지
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• 제21권4호
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• pp.320-326
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• 2004

We have extracted the natural pine-needles to use ethanol in solvent, and could obtain the refined oil component from pine-needles extract. We have tested the antimicrobial effect from microbe experiment and analyzed with ICP/OES, GC/MS. we could obtain the next conclusion from the result of this experiment. In the first result of this experiment we could know that in case of increasing concentration of pine-needles, the number of microbe decreased more and more. Also we could know that the refined oil component of pine-needles appeared the sterilization effect of S-aureus and E-coli after 96hrs and 120hrs. So we could know that the refined oil component of appeared effect to microbe. In the second result of this experiment we could know that inorganic materials of Ca, Mg, V, Mn etcs from pine-needles detected to ICP/OES analysis and the aromatic compounds from refined oil component of pine-needles was certificated to GC/MS analysis.

• 복식문화연구
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• 제18권2호
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• pp.371-381
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• 2010

The pine needles can be used for four seasons in normal living and it can be taken friendly everywhere as it is distributed over 50% in Korea. The pine needles consist of vitamins, protein, minerals, essential oil and enzyme related to antimicrobial activity. It has effect like high blood pressure, neuralgia and hanged over by terpene, glucokinin, rutin, apigenic acid and tannin. Also the extract of them can be used for dyeing of fabrics. However, the extract components and effects of them are not well known yet. Therefore, the purpose of this study was to investigate the volatile components of the pine needles extract and functionality. The pine needles extract was dyed into various fabrics(nylon, silk, wool and soybean) and mordanted with Al, Cu, Cr, Fe and Sn. The extracted aroma compounds were compared by gas chromatography-mass spectrometry. The major volatile compounds of pine needles verified by using SPME were alpha-pinene, beta-pinene, beta-phellandrene, caryophyllene, ethanon, benzen. A total of 15 compounds were identified by using the SPME fibers. In the UV-visible spectra, the maximum absorption of wavelength of the pine needles ethanol extract appeared at 460, 630nm for chlorophyll component and at 237, 281nm for tannin component with the pine needles distilled water extract. Most of sample showed high antibacterial effect in none mordant but wool fabric showed high antibacterial effect in mordants. The result of UV block test showed a superior ability of blocking ultraviolet ray infiltration in all sample.

• 한국의류학회지
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• 제35권2호
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• pp.218-229
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• 2011

Dyeing properties of cotton and ramie fabrics with pine needles colorants were studied by investigating the analysis of pine needles colorants, the effect of dyeing conditions (such as dye concentration, dyeing temperatures and times on dye uptakes), effect of mordants, and color change. The various colorfastness of dyed fabrics were evaluated for practical use; in addition, the antimicrobial ability, ultraviolet-cut ability, and deodorant ability were estimated. In the UV-Visible spectrum, the wavelength of the maximum absorption for pine needles extract was 285nm, and pine needles colorants produced a yellow color. From FT-IR and GC-MS results, it was assumed that chromophoric substance from pine needles extracts were mixed with flavonol tannin and flavanol tannin. An increased dyeing concentration resulted in a larger dye uptake and a Freundlich absorption isotherm was obtained. A larger dye uptake occurred as the dyeing time and temperature increased. Post-mordanting was more effective than pre-mordanting and the dye uptake of fabrics improved by mordanting. The colorfastness of dyed fabrics showed a low rating; however, colorfastness to washing and dry cleaning of cotton fabrics mordanted with N.Cu, and friction fastness of ramie fabric mordanted with Cu improved. The dyed fabrics showed very good antimicrobial abilities of 99.9%. In addition, the ultraviolet-cut ability and deodorant ability improved in fabrics dyed with pine needles extracts.

• 복식문화연구
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• 제17권6호
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• pp.1129-1140
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• 2009

The pine needles has been used as medicines and it is using as dyeing as well as food. It is distributed through 50% in Korean forest. The pine needles is related to antimicrobial activity, however, dyeing properties of the extract components and effects of them are not well known yet. To examine dyeing properties of the pine needles extract, various fabrics(nylon, silk, wool, and soybean) were dyed under different dyeing conditions, and mordanted with one of five mordants(Al, Sn, Fe, Cr, and Cu). Dye uptake, Colors and Colorfastness of the dyed fabrics were measured. By the K/S values, dye uptake of all the dyed fabrics enhanced as increasing dyeing temperature and dyeing time. The highest K/S values were obtained from the protein fiber(wool and silk) fabrics dyed with water extract at $100^{\circ}C$, and with ethanol extract at $90^{\circ}C$ for 80 minutes. Colors of the dyed fabrics showed variety of yellow, brown and green colors. Colors changed by using Cu, Fe and Cr mordants: Cr-mordanted fabrics into light green, Fe-mordanted fabrics into reddish brown, and Cu-mordanted fabrics into deep green in ethanol extraction. Dry-cleaning fastness of the dyed fabrics presented good to excellent except wool fabrics dyed with ethanol extract. Washing fastness of the dyed nylon and soybean fabrics were good to excellent but wool and silk fabrics showed average grades. Most dyed fabrics were poor to light fastness.

• 한국의류학회지
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• 제35권4호
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• pp.466-475
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• 2011

The dyeing properties of protein fiber with pine needles colorants were studied through an investigation of the effect of dyeing conditions such as dye concentration, dyeing temperatures and time on dye uptakes, effect of mordants, and color change. In addition, the various colorfastness of dyed silk and wool fabrics were evaluated for practical use. The antimicrobial ability, ultraviolet-cut ability, and deodorant ability were also estimated. The dye uptake increased as the dyeing concentration increased and this enabled the obtainment of the Langmuir absorption isotherm. The dye uptake increased as the dyeing time and temperature increased. Pine needles colorants showed relatively good affinity to protein fiber and produced a yellow color. Post-mordanting was more effective than the pre-mordanting, and the dye uptake of fabrics improved by mordanting. Except for washing, the colorfastness of dyed fabrics showed a low rating. However, the colorfastness to light and the dry cleaning of fabrics mordanted with N.Cu, and the friction fastness of fabrics mordanted with Cu improved. The guide fabrics showed very good antimicrobial abilities of 99.9%; in addition, the ultraviolet-cut ability and deodorant ability improved in fabrics dyed with pine needles extracts.

• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.227-232
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• 2000

Fresh pine needles were collected and extracted with 95% methanol and the extract was concentrated to determine its antimicrobial activity. The methanol extract had a considerable inhibitory effect on the tested bacteria, such as Esherichia coli 0157;H7, Listeria monocytogenes, Salmonella typhimurium, and Staphylococcus aureus. The methanol extract of pine needles was further fractionated to chloroform, ethylacetate, butanal, and water fractions. Among these four fractions, the butanol and water fractions, which showed a relatively strong inhibitory effect on all of the tested bacteria, were purified and the minimum ingibitory concentration (MIC) was determined for each microorganism. The MIC raged between 25 mg/ml and 45 mg/ml depending on the microorganism. the purified active fractions were applied to sterilized milk as a model food system to define the antimicrobial effectiveness and it was found that the antimicrobial activities in the water fractions were stronger than those in the butanol fractions.

• 패션비즈니스
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• 제14권1호
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• pp.176-183
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• 2010

The purpose of this study is to examine the antibacterial activity and deodorization of fabrics dyed with pine needles extract. The microbes used for a test were two kinds of bacteria and two kinds of fungi. The test results showed as follows. In the test results of antimicrobial activity, both cotton and silk fabrics showed the antimicrobial rate of over 90% after washing three times. In the test result of antifungal activity, all fabrics didn't show any effective antifungal activity. In the test result of deodorization, the dyed cotton fabric showed the higher rate of deodorization than the non-dyed fabric, and the fabric showed the highest rate of deodorization was the test fabric mordanted by Fe and the rate of deodorization showed 92%. In case of silk fabric, the fabric showed the highest rate of deodorization was the test fabric mordanted by Al, and the rate of deodorization showed 92%.

• 한국축산식품학회지
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• 제31권1호
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• pp.74-80
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• 2011

유화형 소시지에 제조 방법을 다르게 추출한 솔잎 추출물을 첨가하여 냉장온도($4{\pm}1^{\circ}C$)에서 30일간 저장하면서 pH, TBARS, 총 미생물수, 육색 및 아질산염 잔존량을 조사하였다. 시험구는 솔잎을 첨가하지 않은 대조구, 솔잎즙 첨가구는 T1, 솔잎 에탄올 추출물 첨가구는 T2, 솔잎중탕 추출물 첨가구를 T3 등 4개 처리구로 나누어 0, 10, 20 및 30일간 저장하면서 실험하였다. pH는 저장기간이 경과하면서 모든 처리구에서 서서히 감소하였다(p<0.05). TBARS와 총미생물수는 저장기간 동안 증가하였으며, 솔잎 즙과 에탄올 추출물을 첨가한 유화형 소시지는 대조구보다 낮아 솔잎의 첨가는 지방산화와 미생물 성장을 지연시켰고, 특히 에탄올 추출물 첨가구인 T2에서 TBARS 값과 미생물수는 가장 낮은 경향이었다(p<005). 육색은 저장기간이 지나면서 CIE $L^*$ 값과 $a^*$ 값 및 $b^*$ 값은 감소하였고, 처리구간에는 CIE $L^*$ 값과 $a^*$ 값은 T1과 T2에서 낮았고, $b^*$ 값은 T1과 T2에서 높은 결과로 솔잎 추출물의 첨가는 소시지 색을 어둡게 하는 경향이었다. 아질산염 잔존량은 저장기간이 경과하면서 감소하였는데 T2에서 다른 시험구보다 유의하게 감소하였다. 이상의 결과를 종합적으로 고찰해보면 솔잎 에탄올 추출물 첨가는 지방의 산화와 미생물 성장 억제 및 아질산염 잔존량 감소에 효과가 가장 있을 것으로 생각된다.