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Genetic variants of the growth differentiation factor 8 affect body conformation traits in Chinese Dabieshan cattle

  • Zhao, Shuanping;Jin, Hai;Xu, Lei;Jia, Yutang
    • Animal Bioscience
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    • v.35 no.4
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    • pp.517-526
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    • 2022
  • Objective: The growth differentiation factor 8 (GDF8) gene plays a key role in bone formation, resorption, and skeletal muscle development in mammals. Here, we studied the genetic variants of GDF8 and their contribution to body conformation traits in Chinese Dabieshan cattle. Methods: Single nucleotide polymorphisms (SNPs) were identified in the bovine GDF8 gene by DNA sequencing. Phylogenetic analysis, motif analysis, and genetic diversity analysis were conducted using bioinformatics software. Association analysis between five SNPs, haplotype combinations, and body conformation traits was conducted in 380 individuals. Results: The GDF8 was highly conserved in seven species, and the GDF8 sequence of cattle was most similar to the sequences of sheep and goat based on the phylogenetic analysis. The motif analysis showed that there were 12 significant motifs in GDF8. Genetic diversity analysis indicated that the polymorphism information content of the five studied SNPs was within 0.25 to 0.5. Haplotype analysis revealed a total of 12 different haplotypes and those with a frequency of <0.05 were excluded. Linkage disequilibrium analysis showed a strong linkage (r2>0.330) between the following SNPs: g.5070C>A, g.5076T>C, and g.5148A>C. Association analysis indicated these five SNPs were associated with some of the body conformation traits (p<0.05), and the animals with haplotype combination H1H1 (-GGGG CCTTAA-) had greater wither height, hip height, heart girth, abdominal girth, and pin bone width than the other (p<0.05) Dabieshan cattle. Conclusion: Overall, our results indicate that the genetic variants of GDF8 affected the body conformation traits of Chinese Dabieshan cattle, and the GDF8 gene could make a strong candidate gene in Dabieshan cattle breeding programs.

Study on the progressive collapse resistance of CP-FBSP connections in L-CFST frame structure

  • Xiong, Qingqing;Wu, Wenbo;Zhang, Wang;Chen, Zhihua;Liu, Hongbo;Su, Tiancheng
    • Steel and Composite Structures
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    • v.44 no.3
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    • pp.437-450
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    • 2022
  • When the vertical load-bearing members in high-rise structures fail locally, the beam-column joints play an important role in the redistribution of the internal forces. In this paper, a static laboratory test of three full-scale flush flange beam-reinforced connections with side and cover plates (CP-FBSP connection) with double half-span steel beams and single L-shaped columns composed of concrete-filled steel tubes (L-CFST columns) was conducted. The influence of the side plate width and cover plate thickness on the progressive collapse resistance of the substructure was thoroughly analyzed. The failure mode, vertical force-displacement curves, strain variation, reaction force of the pin support and development of internal force in the section with the assumed plastic hinge were discussed. Then, through the verified finite element model, the corresponding analyses of the thickness and length of the side plates, the connecting length between the steel beam flange and cover plate, and the vertical-force eccentricity were carried out. The results show that the failure of all the specimens occurred through the cracking of the beam flange or the cover plate, and the beam chord rotations measured by the test were all greater than 0.085 rad. Increasing the length, thickness and width of the side plates slightly reduced the progressive collapse resistance of the substructures. The vertical-force eccentricity along the beam length reduced the progressive collapse resistance of the substructure. An increase in the connecting length between the beam flange and cover plate can significantly improve the progressive collapse resistance of substructures.

Spinal Cord Infarction after C7 Transforaminal Epidural Steroid Injection Using Dexamethasone (덱사메타손을 이용한 경추 7번 경막 외 스테로이드 주사 후 척수 경색)

  • Lee, Jong Hwa;Kim, Young Sam;Kim, Sang Beom;Lee, Kyeong Woo;Kim, Young Hwan
    • Clinical Pain
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    • v.19 no.2
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    • pp.116-119
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    • 2020
  • Cervical transforaminal epidural steroid injection (TFESI) is commonly performed to provide relief of pain caused by radiculopathy. Intra-arterial injection of particulate steroid or direct needle injury can lead to spinal artery embolism or thrombosis. Also there is a possibility of vascular spasm. To our knowledge, this is the first reported case of spinal cord infarction that occurred after TFESI with non-particulate steroid in Korea. A 47-year-old female patient underwent C7 TFESI at local pain clinic. Injected materials were dexamethasone and mepivacaine. Right after the intervention, she felt muscle weakness and decreased sensation. On physical examination, she had decreased sensation from C4 to T2 dermatome in light touch and pin-prick test. Proprioception and vibration were intact. The motor grades of upper extremities were grade 1. Cervical and thoracic spine MRI was checked. Diffusion-weighted image and apparent diffusion coefficient image showed long extension of spinal cord infarction from C2 to T1 level.

Bending characteristics of Prestressed High Strength Concrete (PHC) spun pile measured using distributed optical fibre strain sensor

  • Mohamad, Hisham;Tee, Bun Pin;Chong, Mun Fai;Lee, Siew Cheng;Chaiyasarn, Krisada
    • Smart Structures and Systems
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    • v.29 no.2
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    • pp.267-278
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    • 2022
  • Pre-stressed concrete circular spun piles are widely used in various infrastructure projects around the world and offer an economical deep foundation system with consistent and superior quality compared to cast in-situ and other concrete piles. Conventional methods for measuring the lateral response of piles have been limited to conventional instrumentation, such as electrical based gauges and pressure transducers. The problem with existing technology is that the sensors are not able to assist in recording the lateral stiffness changes of the pile which varies along the length depending on the distribution of the flexural moments and appearance of tensile cracks. This paper describes a full-scale bending test of a 1-m diameter spun pile of 30 m long and instrumented using advanced fibre optic distributed sensor, known as Brillouin Optical Time Domain Analysis (BOTDA). Optical fibre sensors were embedded inside the concrete during the manufacturing stage and attached on the concrete surface in order to measure the pile's full-length flexural behaviour under the prescribed serviceability and ultimate limit state. The relationship between moments-deflections and bending moments-curvatures are examined with respect to the lateral forces. Tensile cracks were measured and compared with the peak strains observed from BOTDA data which corroborated very well. By analysing the moment-curvature response of the pile, the structure can be represented by two bending stiffness parameters, namely the pre-yield (EI) and post-yield (EIcr), where the cracks reduce the stiffness property by 89%. The pile deflection profile can be attained from optical fibre data through closed-form solutions, which generally matched with the displacements recorded by Linear Voltage Displacement Transducers (LVDTs).

Quantitative Assessment of Wear Characteristics of Cr-based Coating Reinforced with Diamond (다이아몬드 강화 Cr 기반 소재의 정량적 마모 특성 평가)

  • Huynh, Ngoc-Phat;Vu, Nga Linh;Chung, Koo-Hyun
    • Tribology and Lubricants
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    • v.38 no.1
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    • pp.15-21
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    • 2022
  • Diamond reinforced Cr-based coating has been proposed as wear-resistant materials. In this study, the friction and wear characteristics of diamond reinforced Cr-based coating are experimentally assessed. The experiments are performed using a pin-on-reciprocating plate tribo-tester under various normal forces with boundary lubrication. The stainless-steel ball is used as a counter material. Prior to the experiments, mechanical properties such as elastic modulus and hardness are determined using nanoscale instrumented indentation. The hardness of the specimen is further determined using a Vickers hardness tester. The specimens before and after the experiments are carefully observed using a confocal microscope to understand the wear characteristics. In addition, the wear volume and wear rate of the specimens are determined based on the confocal microscope data. The results show that the friction coefficients are 0.096-0.100 under 20-40 N normal forces. Furthermore, the wear rates of the diamond reinforced Cr-based coating and the stainless steel ball under 20-40 N normal forces are found to be 12.8 × 10-8 mm3/(Nm)-15.5 × 10-8 mm3/(Nm) and 1.9 × 10-8 mm3/(Nm)-3.9 × 10-8 mm3/(Nm), respectively. However, the effect of the normal force on wear rates is not clearly observed, which may be associated with the flattening of the ball. The results of the study may be useful for the tribological applicability of diamond reinforced Cr-based coating as wear-resistant materials.

The effects of light colour on female rabbit reproductive performance and the expression of key genes in follicular development

  • Xiaoqing, Pan;Xinglong, Wang;Le, Shao;Jie, Yang;Feng, Qin;Jian, Li;Xia, Zhang;Pin, Zhai
    • Journal of Animal Science and Technology
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    • v.64 no.3
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    • pp.432-442
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    • 2022
  • The purpose of this study was to analyse the effects of light colour on rabbit reproductive performance and the expression of key follicular development genes. Rabbits (n = 1,068, 5 months old, 3.6-4.4 kg live body weight) were divided randomly into four groups, housed individually in wire mesh cages and exposed to red, green, blue, and white light-emitting diode (LED) light (control). The lighting schedule was 16 L : 8 D-15 d / 150 lx / 6:00 am-22:00 pm (3 d preartificial insemination to 12 d postartificial insemination). Red light and white light affected the conception rate and kindling rate and increased the total litter size at birth (p < 0.05). The effects of red light on litter size at weaning, litter weight at weaning, and individual weight at weaning increased compared with the green and blue groups. The effects of red light on live litter size at birth were increased compared with those in the blue group (p < 0.05). Compared to white light, green and blue light reduced the number of secondary follicles (p < 0.05). Compared to red light, green and blue light reduced the number of tertiary follicles (p < 0.05). Compared with white light, red LED light resulted in greater ovarian follicle stimulating hormone receptor and luteinizing hormone receptor mRNA expression (p < 0.05). Compared with green and blue LED light, red LED light resulted in greater B-cell lymphom-2 mRNA expression (p < 0.05). Compared with green LED light, red LED light inhibited FOXO1 mRNA expression in rabbit ovaries (p < 0.05). Red light can affect the reproductive performance of female rabbits and the expression of key genes for follicular development.

Standardization of machining process for progressive press die (순차이송형 프레스 금형의 가공표준화)

  • Lee, S.M.;Lee, S.J.
    • Journal of the Korean Society for Precision Engineering
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    • v.10 no.2
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    • pp.114-125
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    • 1993
  • In the present study the newly developed CAD/CAM system is applied to the process of the molding design, machining for mini-sized and precise processive die, and the production of press-stamped parts. When the design of a die was completed by means of CAD, wire cut NC data were generated with the aid of a design drawing in the CAD system and then inputed into the wire cut machine, and with the aid of a hole chart which had been made for this purpose, all the data were classified into the categories of CNC milling, jig boring, jig grinding, and machine center, and then developing a program of generating NC data, errors in process were reduced and programming time was shortened. The program was developed by using Autolisp language which was built-in the CAD, and realizing the intergation of designing a die, generating and processing NC data directly by a designer, designing time and machinery processing time were shorted. And the traditionally required working time for design. NC program required 6 days of work becomes 4 days of work by using the developed CAD/CAM system so that the efficiency shows 150% of the reduction working time. The prpgram of the design of the automation a progressive die mold was developed in the PC-Class Autocad system, therefore development expense could be reduced, and the integration of the CAD/CAM of the progressive die mold with the standard DB being built could be realized.

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Paddy Weeds Serving as the Possible Reservoirs for Rice Bacterial Leaf Blight (답잡초를 대상으로 벼 흰빛잎아름병 기주절위구명)

  • Kim, K.U.;Jeh, S.Y.;Sohn, J.K.;Lee, S.K.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.1
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    • pp.40-44
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    • 1981
  • This study was conducted to identify the possibility of paddy weeds served as the host plant of bacterial leaf blight, using various bacterial groups and inoculation methods. The results obtained can be summarized as follows. 1. Alopecurns spp., Setaria viridis P. Beauv., and Leersia juponica Makino were identified the most susceptible to bacterial leaf blight, similar to Milyang 23 which was used as a susceptible check variety. The others such as Digitaria adscendens Hem., Eleusine indic aGaertin., Cyperns serotinus Rottb, Cyperns difformis L. showed slight infection but most of broadleaf weeds were resistant to bacterial leaf blight. 2. Weed species showing early susceptibility maintained their susceptibility throughout the growth stages. Group I of bacterial leaf blight was the most effective to develop infection symptom to weeds. 3. Pin and scissor inoculation methods were more effective mean for infection than spray method which was used without wound.

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Determination of Peening Area for Finite Element Residual Stress Analysis of Ultrasonic Nanocrystal Surface Modification under Multiple Impact Conditions (초음파나노표면개질 다중충격 조건에서의 잔류응력 예측을 위한 유한요소 피닝해석 영역 결정)

  • Tae-Hyeon Seok;Seung-Hyun Park;Nam-Su Huh
    • Transactions of the Korean Society of Pressure Vessels and Piping
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    • v.17 no.2
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    • pp.145-156
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    • 2021
  • Ultrasonic Nanocrystal Surface Modification (UNSM) is a peening technology that generates elastic-plastic deformation on the material surface to which a static load of a air compressor and a dynamic load of ultrasonic vibration energy are applied by striking the material surface with a strike pin. In the UNSM-treated material, the structure of the surface layer is modified into a nano-crystal structure and compressive residual stress occurs. When UNSM is applied to welds in a reactor coolant system where PWSCC can occur, it has the effect of relieving tensile residual stress in the weld and thus suppressing crack initiation and propagation. In order to quantitatively evaluate the compressive residual stress generated by UNSM, many finite element studies have been conducted. In existing studies, single-path UNSM or UNSM in a limited area has been simulated due to excessive computing time and analysis convergence problems. However, it is difficult to accurately calculate the compressive residual stress generated by the actual UNSM under these limited conditions. Therefore, in this study, a minimum finite element peening analysis area that can reliably calculate the compressive residual stress is proposed. To confirm the validity of the proposed analysis area, the compressive residual stress obtained from the experiment are compared with finite element analysis results.

DEVELOPMENT OF ERP INTEGRATION SYSTEM FOR SPORTS INDUSTRY IN TAIWAN

  • Yan-Chyuan Shiau;Yu-Min Hsu;Shu-Jen Sung;Chih-Kun Chu;Hsiang-Lun Cheng;Tsung-Pin Tsai
    • International conference on construction engineering and project management
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    • 2005.10a
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    • pp.1028-1035
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    • 2005
  • Traditional Industries were planning the major role for Taiwan's economy. However, we need to face the competition from developing countries. Moving manufacturing department to other low salary countries, such as China, can only temporarily release some limited pressure. The final and complete solutions are to equip on R&D, refine techniques, improve management capabilities, upgrade business, and reform physique. Currently there are some ERP systems on the market; however, they are designed for common purpose and difficult to introduce into industry. The expensive price is another factor to make them be popular. In this research we will Object Oriented, Visual Modeling, ER Model and Windows Environment to develop an Integrated Management System for Sports Requisites Industries (IMSSRI). We will integrate all information from all departments such as development, business, material administration, manufacture, shipping, and financial. When development people construct the all modules, components, cutting molds, materials and accessories, IMSSRI will calculate all needed materials and cost for each product. This cost will be used for quotation to report to our customers. When customers confirm the order, system will transfer all necessary materials into Material Administration System. IMSSRI can generate manufacturing forms and material raw lists for manufacture department. All related information such as stocking, returning goods, material requesting, and material returning can be integrated so we can control all details of the whole enterprise. Through the help of this system, we hope we can save man-power, reduce human mistakes, raise management capabilities for tradition manufacturing industries and create another possibility of eternal operating for Taiwan's Industries.

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