• Archives of Plastic Surgery
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• 제34권5호
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• pp.659-662
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• 2007

Purpose: Multiple skin leiomyoma and uterine myoma bearing autosomal dominant traits are benign smooth muscle tumors which originate in skin or female uterus. Skin leiomyoma occurs after gene mutation originating from arrector pili muscle of hair follicle where its clinical manifestations vary significantly from person to person. Our department hereby reports the histological findings and genetic evaluations of this very rare disease. Methods: A 57-year-old woman presented in our institute with multiple tumors in the left and central parts of her back that started to appear since 19 years ago. The patient was diagnosed as having uterine myoma 15 years ago and underwent hysterectomy. Biopsy has been done on the specimen, and genomic DNA was separated from Fumarate hydratase gene for it to go through PCR amplification. The results of PCR amplification were aligned by sequencer. Results: According to the results of biopsy, tumor cells were spindle-shaped and were aligned in a bundle where there was no dysplasia or mitosis. Moreover, these cells had abundant eosinophilic cytoplasm with elongated nucleus, and benign leiomyoma that showed positive reactions to SMA stain were found. In genetic examination, mutations such as heterozygous single nucleotide substitutions were found in alignments of amplified DNA. Conclusion: Multiple skin leiomyoma and uterine myoma are relatively uncommon diseases that are transmitted through autosomally dominant traits from genetic mutations. When a patient's chief complaint lies upon skin-colored or brown masses that occur in multiples appearing in the trunk or extremities with characteristic clinical symptoms and histological findings, and when the patient's family history is acknowledged such as skin or uterine leiomyoma or renal tumor, necessary genetic examination on multiple skin leiomyoma and uterine myoma could be done, and thereby precise diagnosis could also be made.

• Journal of Plant Biotechnology
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• 제32권4호
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• pp.233-241
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• 2005

Plants have considerable advantages for the production of antigenic proteins because they provide an inexpensive source of protein and an easy administration of vaccine. Since a publication describing edible plant vaccine of HBsAg in 1992, a number of laboratories around the world have studied the use of plants as the bioreactor to produce antigenic proteins of human or animal pathogens. Over the last ten years, these works have been mainly focused on three major strategies for the production of antigenic proteins in plants: stable genetic transformation of either the nuclear or plastid genome, or transient expression in plants using viral vectors. As many antigenic proteins have been expressed in tobacco, also several laboratories have succeeded to express genes encoding antigenic proteins in other crop plants: potato, tomato, maize, carrot, soybean and spinach. At present many works for the production of edible plant vaccine against bacteria-mediated diseases have mostly performed the studies of enterotoxins and adhesion proteins. Also the development of new-type antigens (pili, flagella, surface protein, other enterotoxin and exotoxin etc.) is required for various targets and more efficacy to immunize against microorganism pathogens. Many works mostly studied in experimental animals had good results, and phase I clinical trial of LTB clearly indicated its immunogenic ability. On the other hand, edible plant vaccines have still problems remained to be solved. In addition to the accumulation of sufficient antigen in plants, human health, environment and agriculture regulation should be proven. Also oral tolerance, the physiological response to food antigens and commensal flora is the induction of a state of specific immunological unresponsiveness, needs to be addressed before plant-derived vaccine becomes a therapeutic option.

• 한국식품과학회지
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• 제49권1호
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• pp.28-34
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• 2017

본 연구를 통하여 P. aeruginosa PR3를 이용하여 DOD를 생산하기 위해 저가의 기질로서 필리핀 너트유가 효과적으로 사용될 수 있음을 확인하였으며 배지에 첨가되는 여러 영양인자들의 영향을 조사하여 DOD 생산성을 크게 향상시킬 가능성이 있음도 확인하였다. 따라서 DOD 생산에 이용되는 올레산을 식물성오일로부터 별도의 생산과정을 거쳐 생산하지 않고 식물성오일자체를 직접 기질로 사용함으로서 PR3 균주를 이용하여 고부가가치의 DOD를 효율적으로 생산할 수 있다는 것을 확인하였다.

• Journal of Animal Science and Technology
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• 제61권2호
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• pp.55-60
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• 2019

Colibacillosis is one of the major health problems in young piglets resulting in poor health and death caused by Escherichia coli producing F18 pili and Shiga toxin 2e. It is pivotal to reduce colibacillosis in weaned piglets to enhance production performance. In this study, we evaluated synbiotics as the gut health improvement agents in the mouse model challenged with Shiga toxin-producing E. coli (STEC) isolated from piglets. Prebiotic lactulose was formulated with each $5.0{\times}10^6CFU/mL$ of Pediococcus acidilactici GB-U15, Lactobacillus plantarum GB-U17, and Lactobacillus plantarum GB 1-3 to produce 3 combinations of synbiotics. A total of 40 three weeks old BALB/c mice were randomly assigned to 4 groups (n = 10): a control group and 3 synbiotics treated groups. Each treatment groups were daily administrated with $5.0{\times}10^6CFU/mL$ of one synbiotics for the first week, and every 3 days during the second week. All the mice were challenged with $8.0{\times}10^8CFU/mL$ of STEC 5 days after animals began to receive synbiotics. Mice treated with synbiotics based on Pediococcus acidilactici GB-U15 and Lactobacillus plantarum GB-U17 significantly improved daily weight gain compared to mice in other groups. While mice treated with GB-U15 showed better fecal index, no significant differences were observed among groups. Gross lesion and histopathological evaluations showed that mice treated with GB-U15 moderately improved recovery from STEC infection. In conclusion, our results suggest that the synbiotics formulated with lactulose and Pediococcus acidilactici GB-U15 have potential benefits to prevent and improve colibacillosis in weaned piglets.

• Childhood Kidney Diseases
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• 제6권1호
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• pp.75-84
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• 2002

목 적 :성장기 신장에서의 급성신우신염은 신반흔으로 진행된다. 신반흔의 형성에는 세균자체보다도 숙주의 염증반응과 면역반응의 산물인 TGF-${\beta}1$이 세포 고사를 증가시키고 세포증식을 억제함으로서 섬유화를 촉진한다고 하였다. 이에 저자는 항염증제인 methyl-prednisolone (MP)이 실험적으로 급성신우신염을 일으킨 이유기 백서에서 신반흔 형성에 미치는 영향을 관찰하고자 하였다. 대상 및 방법 : 생후 3주(체중 50-60g)된 이유기 Sprague-Dawley 백서의 방광에 삽입된 16 guage의 실리콘 도관내로 107/mL 농도의 E coli (ATCC No. 25922, pili형)를 5 mL씩 주입하여 급성신우신염을 유발하였다. 실험군은 1군 (ceftriaxone 단독투여, n=31)과 2군 (MP와 ceftriaxone투여, n=28)으로 나누었고 대조군 (n=43)에는 약제를 투여하지 않았다. 실험 1주와 3주에 실험동물을 희생하여 병리 조직학적 소견상 염증점수, 세포고사 지수와 TGF-${\beta}1$ 발현점수 및 섬유화 점수를 관찰하였다. 결 과 : 사망률은 II군이 $21.4\%$였으나 대조군 $41.9\%$, I군 $32.3\%$와 유의한 차이는 없었다. 염증 점수는 실험 1주에 II군에서 $0.8{\pm}0.87$로 대조군의 $2.3{\pm}0.87$, I군의 $1.7{\pm}0.79$에 비하여 유의하게 낮았다 (P<0.05. 세포고사 지수는 실험 1주에 II군에서 $2.9{\pm}2.15$로 대조군의 $10.0{\pm}1.95$, I군의 $8.3{\pm}2.53$에 비하여 유의 하게 낮았다 (P<0.05). TGF-${\beta}1$발현도 실험 1주에 II군에서 $0.8{\pm}0.72$로 대조군의 $1.90{\pm}67$, I군의 $1.8{\pm}0.60$에 비하여 유의하게 낮았다 (P<0.05). 섬유화 지수는 실험 3주에 II군에서 $0.8{\pm}0.63$로 대조군의 $1.8{\pm}0.83$에 비하여 유의하게 낮았다 (P<0.05) 결 론 : 성장기 백서의 실험적 급성 신우신염에서 MP는 ceftriaxone단독 투여에 비하여 염증 반응, 세포고사, TGF-${\beta}1$발현, 섬유화를 모두 감소 시켰다. 즉 항생제 외에 항염증제의 병용투여가 신반흔의 정도를 감소시킬 수 있으므로 치료지연등 신반흔의 위험인자가 있는 경우에 고려할 수 있을 것으로 생각된다.