Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Evaluation of synbiotics as gut health improvement agents against Shiga toxin-producing Escherichia coli isolated from the pig

  • Kim, Bo-Ra (Department of Animal Resources Science, Dankook University) ;
  • Cho, Kyung Jin (Genebiotech Co., Ltd.) ;
  • Kim, Doowan (National Institute of Animal Science, Rural Development Administration) ;
  • Cho, Jin Ho (Division of Food and Animal Sciences, Chungbuk National University) ;
  • Lee, Jun Hyung (Department of Animal Resources Science, Dankook University) ;
  • Guevarra, Robin B. (Department of Animal Resources Science, Dankook University) ;
  • Lee, Sun Hee (Department of Animal Resources Science, Dankook University) ;
  • Kang, Jung Sun (Genebiotech Co., Ltd.) ;
  • Cho, Won Tak (Genebiotech Co., Ltd.) ;
  • Wattanaphansak, Suphot (Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University) ;
  • Kang, Bit Na (Abbvie Bioresearch Center) ;
  • Kim, Jong Nam (Department of Beef Science, Korea National College of Agriculture and Fisheries) ;
  • Song, Minho (Division of Animal and Dairy Science, Chungnam National University) ;
  • Kim, Hyeun Bum (Department of Animal Resources Science, Dankook University)
  • Received : 2019.01.21
  • Accepted : 2019.01.29
  • Published : 2019.03.31
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Abstract

Colibacillosis is one of the major health problems in young piglets resulting in poor health and death caused by Escherichia coli producing F18 pili and Shiga toxin 2e. It is pivotal to reduce colibacillosis in weaned piglets to enhance production performance. In this study, we evaluated synbiotics as the gut health improvement agents in the mouse model challenged with Shiga toxin-producing E. coli (STEC) isolated from piglets. Prebiotic lactulose was formulated with each $5.0{\times}10^6CFU/mL$ of Pediococcus acidilactici GB-U15, Lactobacillus plantarum GB-U17, and Lactobacillus plantarum GB 1-3 to produce 3 combinations of synbiotics. A total of 40 three weeks old BALB/c mice were randomly assigned to 4 groups (n = 10): a control group and 3 synbiotics treated groups. Each treatment groups were daily administrated with $5.0{\times}10^6CFU/mL$ of one synbiotics for the first week, and every 3 days during the second week. All the mice were challenged with $8.0{\times}10^8CFU/mL$ of STEC 5 days after animals began to receive synbiotics. Mice treated with synbiotics based on Pediococcus acidilactici GB-U15 and Lactobacillus plantarum GB-U17 significantly improved daily weight gain compared to mice in other groups. While mice treated with GB-U15 showed better fecal index, no significant differences were observed among groups. Gross lesion and histopathological evaluations showed that mice treated with GB-U15 moderately improved recovery from STEC infection. In conclusion, our results suggest that the synbiotics formulated with lactulose and Pediococcus acidilactici GB-U15 have potential benefits to prevent and improve colibacillosis in weaned piglets.

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References

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