• Title/Summary/Keyword: pigment composition

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A Study on Mineralogical Characteristic and Accelerated Weather Test of Red and Yellow Color Natural Inorganic Pigments (적색 및 황색 계열의 천연 무기안료의 광물학적 특성 및 촉진내후성 평가 연구)

  • Park, Ju Hyun;Jeong, Hye Yeong
    • Journal of the Mineralogical Society of Korea
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    • v.32 no.4
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    • pp.259-271
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    • 2019
  • We evaluated mineralogical, physical property and accelerated weather resistance of cinnabar, realgar and orpiment used as cultural heritages and traditional paintings. JB and JS are sample names of cinnabar and JH and UH are sample names of orpiment and Realgar, respectively. As a result of mineralogical property evaluation, Jinsa was identified cinnabar (HgS) and there was no difference in chemical composition according to the grade. UH and JH were confirmed realgar (AsS) and orpiment (As2S3), respectively. The polarization characteristics such as shape and color indicate that pigments using our test were natural mineral pigment observed by polarization microscope. Especially, in the case of cinnabar, it is not easy to distinguish between natural mineral pigment and synthetic pigment. But the results of polarization microscope said that cinnabar is natural occurring mineral which have authentic mineral particle and unevenly fracture. As a result of thermal analysis, JH has a higher glass transition temperature and heat stability than UH. After accelerated weather test, cinnabar, realgar and orpiment were striking result in color change because of light degradation. Red color of cinnabar turn into black and the color of realgar and orpiment became brighter than before. JB (meta-cinnabar) is more photosensitivity and faster becoming dark than JB (cinnabar). Finally light transforms realgar (red, As4S4) in arsenolite (As2O3).

Isolation and Characterization of Pure lines of Pigmentation and Morphological Mutants in Porphyra tenera Kjellman (Bangiales, Rhodophyta) (참김 (Porphyra tenera Kjellman) 색소 및 형태변이체의 순계주 분리 및 특성)

  • Hwang, Mi-Sook;Kim, Seung-Oh;Lee, Young-Soon;Park, Eun-Jeong;Kim, Seong-Cheol;Ha, Dong-Soo;Gong, Yong-Gun;Baek, Jae-Min;Choi, Han-Gu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.5
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    • pp.495-502
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    • 2010
  • Pure lines were isolated from young gametophytic blades of pigmentation and morphological mutants in Porphyra tenera. Growth, blade-shape and photosynthetic pigment content of pure lines were compared with the wild type. Growth of blade length in the wild type (W, R-B), with round shape and brown color, was fastest at $5{\sim}10^{\circ}C$ and became slower as temperature increased. The blade-shape of the wild type changed from linear to round as temperature increased. The green type (R-G), with round shape and green color, showed slower growth, and the red type (R-R) 'with round shape and red color' showed faster growth than the wild type. The blade-shapes of the green and red types changed from elliptical or linear to round as temperature increased. The phycoerythrin (PE) / phycocyanin (PC) ratio of the green type was markedly lower and the PE/PC ratio of the red type was markedly higher than that of the wild type. The linear type (L-B), with liner shape and brown color, showed faster growth in blade length than the wild type at $10{\sim}20^{\circ}C$ and maintained its linear shape at $5{\sim}15^{\circ}C$. The content of photosynthetic pigments of the linear type was similar to that of the wild type. Each of the pure lines of pigmentation and morphological mutants that were isolated in the present study showed particular patterns in growth, blade-shape and photosynthetic pigment composition. Therefore, they are expected to be useful as new varieties by themselves and to be available for breeding and biotechnological studies.

The Pigments Variation of Phytoplankton in the Seomjin and Yeongsan River estuary (섬진강과 영산강 하구의 식물플랑크톤 기원 색소분포 변동)

  • Jeon, Hyeji;Lee, Eugene;Son, Moonho
    • Journal of Marine Life Science
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    • v.5 no.2
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    • pp.99-106
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    • 2020
  • To investigate effect of variation in physiochemical conditions due to river discharge on phytoplankton, field surveys were conducted in the Seomjin and Yeongsan River estuaries from April to November 2016. The concentrations of DIN and DSi in Seomjin River estuary were gradually low as distance from upstream. On the other hands, the concentrations of DIN and DSi in Yeongsan River estuary were critically high at upstream, due to which is characterized as semi-enclosed eutrophic area. A total of 12 phytoplankton pigments were analyzed, and the distribution of each taxa was investigated using indicator for each phytoplankton taxa. Fucoxanthin, an indicator pigment of diatoms, showed an average of 0.61±1.00 ㎍ l-1 and 0.76±1.22 ㎍ l-1 in the Seomjin and Yeongsan River estuaries, respectively. Concentration of fucoxanthin was more than twice that of other pigments except chlorophyll a., indicating that diatoms were dominant taxa. Peridinin, an indicator pigment of dinoflagellate, showed some similar tendency to the microscopic observation, but mismatch results were also present, indicating a technical limitation of pigment analysis. Chlorophyll b, alloxanthin, and zeaxanthin, which are indicator pigments of green algae, cryptomonads, and cyanobacteria, were detected in both estuaries even though those taxa were not detected in microscopic observation. This indicates that the two estuaries were affected by freshwater species. Here, we can suggest that phytoplankton composition in estuary was directly influenced by the inflow from upstream. In particular, the phytoplankton population dynamics in Yeongsan River estuary was greatly associated with a large-scale artificial dyke, especially in summer rainy season. On the other hands, the seasonal and horizontal distribution of phytoplankton in Seomjin River estuary has changed along the salinity gradients and inflow-related changes.

The Discoloration Characteristics of Orpiment used as Traditional Yellow Mineral Pigments in Painting Cultural Properties (채색문화재에 적용된 전통 황색 광물안료 석황의 채색 특성)

  • Jin Young Do
    • Korean Journal of Mineralogy and Petrology
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    • v.36 no.1
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    • pp.1-17
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    • 2023
  • On painting cultural properties to which Orpiment, a traditional yellow mineral pigment, is applied, the color's degeneration is frequently observed. To identify the cause of the degeneration, this study takes a look into orpiment and the various pigments that are mixed into it (Lead White, Lead Red, and Cinnabar) in powder and painting state. The pigment was mixed with Argyo glue and then applied to korean traditional paper and silk. Considering the possibility that alum causes the discoloration, it was applied to the specimen. With a UV tester, the powders and the painted specimens were subjected to a light resistance test in three phases (96 hour). Color changes were measured with a colorimeter and minerals, chemical composition and structural changes were analysed by XRD, SEM/EDS and Raman spectrometers. While the color change of pure Orpiment powder according to the light resistance test was small, the colored specimen became darker. The color change was large in the Orpiment colored on the silk and in the alum-treated specimen. In Orpiment powder was produced white arsenolite as altered orpiment after UV test. In the mixed powder of Orpiment and Lead White were detected only the constituent minerals of Orpiment and Lead White, and no altered substances were produced. Whereas after the UV test, orpiment and arsenolite, which were altered substances of orpiment, and the constituent minerals of Lead White were detected. In the case of mixing the two pigments in the powder state, darkening did not occur even by the UV test. However, the specimens colored with the mixed powder were darkened by the UV test. The color change of Orpiment was different depending on the mixed pigment and base material. The color change was greater in the case of alum treatment than in the case without alum treatment, and it was found that alum also had an influence on the color change of Orpiment.

Transgenic tobacco with γ-TMT of perilla showed increased salt resistance and altered pigment synthesis (들깨 γ-TMT 형질전환 담배의 색소성분 변화 및 염 스트레스 내성 증가)

  • Woo, Hee-Jong;Sung, Jwa-Kyung;Kim, Jung-Bong;Kim, Na-Young;Lee, Si-Myung;Shin, Kong-Sik;Lim, Sun-Hyung;Suh, Seok-Cheol;Kim, Kyung-Hwan;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.35 no.4
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    • pp.329-335
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    • 2008
  • Tocopherols are essential lipophilic antioxidant in human cells, while little is known about its function in plant tissues. To study the impact of composition and content of tocopherols on stress tolerance, tobacco (Nicotiana tabacum) was transformed with a construct containing a cDNA insert encoding $\gamma$-tocopherol methyltransferase ($\gamma$-TMT/VTE4) from perilla under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco was confirmed by PCR and RT-PCR. The total content and composition of tocopherols in the transgenic lines were similar with wild type controls. However, chlorophyll-a and carotenoid content in the transgenic lines were increased by up to 45% (P<0.01) and 39% (P<0.02), respectively. Also, the over-expression of $\gamma$-TMT increased the salt stress tolerance in tobacco plants. These results demonstrate that over-expression of $\gamma$-TMT gene in tocopherol bio-synthetic pathway can increase salt stress tolerance and contents of chlorophyll-a and carotenoid in transgenic tobacco plants.

Effects of Washing and Desinewing Treatments on the Composition and Quality Characteristics of Spent Layer Meat (세척 및 결체조직 제거 처리가 노계육의 성분 및 품질 특성에 미치는 영향)

  • Yi, Song-Sop;Mast, Morris G.
    • Korean Journal of Food Science and Technology
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    • v.23 no.6
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    • pp.702-710
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    • 1991
  • The effects of washing and desinewing on the composition, fuctional properties, storage stability and texture of spent layer meat were investigated. Spent layer meat subjected to treatments involving water washing, bicarbonate washing and desinewing showed increases in moisture content and decreases in protein content, hydroxyproline content and heme pigment content. Increases in salt extractable protein content and water holding capacity and decreases in buffering capacity and emulsifying capacity were also observed. The 2-thiobarbituric acid values of washed and desinewed samples increased slowly during storage indicated the increase in storage stability compared to the control. Sample rolls prepared from bicarbonate washed and desinewed thigh and drumstick meats were scored by trained sensory panelists as less tough than the products made of ground thigh and drumstick meats.

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HPLC Analysis of Biomass and Community Composition of Microphytobenthos in the Saemankeum Tidal flat, West Coast of Korea (한국 서해 새만금 갯벌에서 저서미세조류의 생체량과 군집조성에 대한 HPLC 분석)

  • OH Seung-Jin;MOON Chang-Ho;PARK Mi-Ok
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.3
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    • pp.215-225
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    • 2004
  • Biomass and community composition of microphytobenthos in the Saemankeum tidal flat were studied by HPLC analysis of the photosynthetic pigments from November 2001 to November 2002. The environmental factors of sediment were also investigated to examine the relationship between microphytobenthos biomass and sedimentary environments. The detected photosynthetic pigments of microphytobenthos were chlorophyll a, b, c, fucoxanthin, 19'-hexanoyloxyfucoxanthin, violaxanthin, diadinoxanthin, alloxanthin, diatoxanthin, zeaxanthin+lutein, peridinin and beta-carotene. Pheophytin a, the degradation product of chlorophyll a, was also detected. The results of pigmen analysis suggest the presence of diatom (fucoxanthin), euglenophytes (chlorophyll b), chlorophytes (chlorophyll b + lutein), cyanobacteria (zeaxanthin), cryptophytes (alloxanthin), chrysophytes (fucoxanthin + violaxanthin), prymnesiophytes (19'-hexanoyloxyfucoxanthin) and dinoflagellates (peridinin). Chlorophyll a concentration in the top 0.5 cm of sediment was in the range of $0.24\;mg{\cdot}m\^{-2}\;-32.11\;mg{\cdot}m\^{-2}$ in the study area. The increase of chlorophyll a concentration in the spring indicates the occurrence of a microphytobenthic bloom. In the summer, there was a sharp decrease of the chlorophyll a concentration which was probably due to high grazing activity by macrobenthos. The annual mean chlorophyll a concentration in the study area was low compared to that in most of other tidal flat areas probably due to active resuspension of microphytobenthos and high grazing activity by macrobenthos. There was no clear relationship between microphytobenthos biomass and sedimentary environments because of a large variety of physical, chemical and biological factors, Pigment analysis indicated that while diatoms were dominated in the microphytobenthic community of the Geojon tidal flat, euglenophytes and/or chlorophytes coexisted with diatoms in the Mangyung River tidal flat.

MERCURY-INDUCED ALTERATIONS OF CHLOROPHYLL a FLUORESCENCE KINETICS IN ISOLATED BARLEY (Hordeum vulgare L. cv. ALBORI) CHLOROPLASTS

  • Chun, Hyun-Sik;Lee, Choon-Hwan;Lee, Chin-Bum
    • Journal of Photoscience
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    • v.1 no.1
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    • pp.47-52
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    • 1994
  • Effects of HgCl$_2$-treatment on electron transport, chlorophyll a fluorescence and its quenching were studied using isolated barley (Hordeum vulgare L. cv. Albori) chloroplasts. Depending on the concentration of HgCI$_2$, photosynthetic oxygen-evolving activities of photosystem II (PS II) were greatly inhibited, whereas those of photosystem I (PS I) were slightly decreased. The inhibitory effects of HgCl$_2$ on the oxygen-evolving activity was partially restored by the addition of hydroxyamine, suggesting the primary inhibition site by HgCl$_2$2-treatment is close to the oxidizing site of PS tl associated with water-splitting complex. Addition of 50 $\mu$M HgCI$_2$ decreased both photochemical and nonphotochemical quenching of chlorophyll fluorescence. Especially, energy dependent quenching (qE) was completely disappeared by HgCl$_2$-treatment as observed by NH$_4$CI treatment. In the presence of HgCI$_2$, F'o level during illumination was also increased. These results suggest that pH gradient across thylakoid membrane can not be formed in the presence of 0 $\mu$M HgCl$_2$. In addition, antenna pigment composition might be altered by HgCl$_2$-treatment.

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A Study on Aqueous Gravure Ink for PVC Film Printing (PVC Film 인쇄용 수성 그라비어 잉크에 관한 연구)

  • Oh, Jong-Min;Ahn, Suk-Chul;Nam, Su-Yong
    • Journal of the Korean Graphic Arts Communication Society
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    • v.22 no.2
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    • pp.199-211
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    • 2004
  • The drying speed of gravure ink are very fast because of evaporation drying type. The gravure ink are fitly selected variety resin and solvent for a wide range printing are adopted. Moreover, after-manufacture handling is necessary to various purposes. Though composition of gravure ink are same those of another printing ink, the viscosity of it are vary low compare with another printing inks. As property of variety printed matters, improvement of performance of printing layer and suitable after-manufacture handling, the more research of gravure ink are to be continue for development of printing technology. Generally, the gravure ink are made up resin, pigment, solvent and additive. Especially solvent are fitly selected for solution of resin, viscosity, and drying speed. Furthermore, it tis care of bad smell of remaining resin, poison and danger of fire of it. Because of the prevention of air pollution as restriction deflation of solvent, custody of dangerous object and improvement of work environment as labor safety hygiene and fire-low, the environment restriction of printing business become strict. This study has purpose of development of gravure ink of involved 30% below of alcohol is used for PVC film printing.

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Composition in Carotenoids of Rainbow Trout Fed with CLA and Ascidian Tunic Extracts (CLA와 멍게 껍질 추출물 급이에 따른 무지개송어 색소조성)

  • Kang, Seok-Joong;Choi, Byeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.6
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    • pp.721-724
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    • 2009
  • Astaxanthin is a valuable pigment source for many aquacultured species, including salmonoids, shrimp, sea bream, and ornamental species. Conjugated linoleic acid (CLA) and ascidian tunic extracts were mixed with the basal diet of rainbow trout to investigate their pigmentation effects. Synthetic Carophyll Pink and natural carotenoids that came from the tunic extracts were incorporated into muscle and skin tissues. The main carotenoids found in muscle after 8 weeks were canthaxanthin in CP12 (13.4%), and CP52 (17.2%), and astaxanthin in CP12 (58.5%), and CP52 (59.2%) in the Carophyll Pink group, while those in skin were canthaxanthin in CP14 (34.5%), and CP54 (29.2%), and astaxanthin in CP14 (32.0%), and CP54 (36.5%) in the ascidian tunic extract group. The total carotenoid content in skin (53.0-69.3 mg/kg) was greater than that in muscle (9.5-13.8 mg/kg).