• 한국동물위생학회지
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• 제35권2호
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• pp.83-90
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• 2012

Porcine rotaviruses are the most common causes of viral gastroenteritis in piglets around the world. The major G genotypes of porcine rotaviruses causing diarrhea were G4, G5 and G11 genotypes. Recently, G9 genotype rotaviruses were problemed at swine farms and frequently recognized from diarrheic piglets. In this study, a porcine rotavirus (PoRV-1) was isolated from piglet showing diarrhea using MA104 cells and confirmed as rotavirus by electron microscopy, genomic RNA electropherotyping and indirect immunofluorescence antibody tests. The nucleotide sequence of the VP7 gene of PoRV-1 was determined and compared with those of other genotype rotavirus strains from other parts of the world. Also, the nucleotide sequences of VP4, VP6 and NSP4 genes of PoRV-1 were determined and compared with those of other rotavirus strains from other countries. The results showed that the PoRV-1 isolate belonged to the G9 genotype and the P, I and E genotypes of PoRV-1 were P[23], I5 and E1, respectively. The Korean G9 PoRV-1 isolate and its nucleotide sequence data would be usefully used for the development of porcine rotavirus vaccines in near future.

• 한국동물위생학회지
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• 제42권1호
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• pp.9-15
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• 2019

Transmissible gastroenteritis (TGE) is a disease confined to pigs of all ages, and can be a significant cause of economic loss in breeding herds, primarily because of the very high piglet mortality. The causative agent is a coronavirus, an enveloped positive strand RNA virus and closely related but non-enteropathogenic porcine respiratory coronavirus (PRCV). Although the TGEV has declined with its innocent relative, PRCV, further genome changes could not be excluded. Therefore, the herd-level immunity against this virus is important for the prevention of disease and should be carefully monitored. The aim of this study is to develop monoclonal antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) which can rapidly and accurately determine a large numbers of serum samples for surveillance purpose, and to compare the ELISA with a TGEV-specific serum neutralization test. The MAC-ELISA was sufficiently achieved, and the comparison with the virus-specific serum neutralization assays for 713 sera from pig farms showed a high correlation ($r^2=0.812$, P<0.001). The specificity and sensitivity of MAC-ELISA for the serum neutralization test 91.9% and 91.6%, respectively, which means that the antibody detected by the MAC-ELISA could be said to be protective antibodies. In conclusion, the developed MAC-ELISA would be very helpful in evaluating protective antibodies against TGEV.

• 농업과학연구
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• 제49권2호
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• pp.297-306
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• 2022

This experiment was conducted to evaluate the effects of δ-aminolevulinic acid (ALA) when added to sows' diet on their reproductive performance and growth performance and on the hematology parameters of the sows and their piglets. Sixteen multiparous sows (Yorkshire × Landrace) were allotted into two treatment groups and fed basal diets (CON, piglets were injected with iron dextran) or the basal diet containing 0.1% ALA (ALA, piglets were not injected with iron dextran) from day 100 of gestation to day 28 of lactation. Supplementation of ALA had no effect on the body weight (BW), backfat thickness (BFT), or litter sizes of sows in the present experiment. However, the average daily feed intake (ADFI) of the sows was significantly improved (p < 0.05) in the ALA group. Supplementation of ALA had no effect on the growth performance or survival of suckling piglets but had a significant effect on the birth weight (p < 0.05). With regard to the blood profiles, serum concentrations of iron were unaffected in sows and piglets as compared to the control group. Red blood cell (RBC) counts were significantly improved (p < 0.05) in sows during late gestation to the time before farrowing period and in piglets at weaning. In summary, these results suggest that dietary supplementation of ALA can have positive effects by improving growth performance and blood RBC in sows and suckling piglets.

• 한국농공학회논문집
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• 제65권1호
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• pp.61-72
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• 2023

The increase in the rearing intensity of pigs has caused deterioration in the pig house's internal environment such as temperature, humidity, ammonia gas, and so on. Traditionally, the widely used method to control the internal environment was through the manipulation of the ventilation system. However, the conventional ventilation system had a limitation to control the internal environment, prevent livestock disease, save energy, and reduce odor emission. To overcome this problem, the air-recirculated ventilation system was suggested. This system has a semi-closed loop ventilation type. For designing this system, it was essential to evaluate the ventilation rates considering the pressure loss of ducts. Therefore, in this study, pressure loss calculation and experiment were conducted for the quantitative ventilation design of a semi-closed loop system. The results of the experiment showed that the inlet through which external air flows should always be opened. In addition, it was also found that for the optimum design of the semi-closed loop ventilation system, it was appropriate to install a damper or a backflow prevention device rather than a ventilation fan.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.449-456
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• 2008

Phenotypic screening for bile salt hydrolase (BSH) activity was performed on Lactobacillus acidophilus PF01 isolated from piglet feces. A gene encoding BSH was identified and cloned from the genomic library of L. acidophilus PF01. The bsh gene and surrounding regions were characterized by nucleotide sequence analysis and were found to contain a single open reading frame (ORF) of 951 nucleotides encoding a 316 amino acid protein. The potential bsh promoter region was located upstream of the start codon. The protein deduced from the complete ORF had high similarity with other BSHs, and four amino acid motifs located around the active site, FGRNXD, AGLNF, VLTNXP, and GXGXGXXGXPGD, were highly conserved. The bsh gene was cloned into the pET21b expression vector and expressed in Escherichia coli BLR(DE3) by induction with 0.1mM of isopropylthiogalactopyranoside. The BSH enzyme was purified with apparent homogeneity using a $Ni^{2+}$-NTA agarose column and characterized. The overexpressed recombinant BSH enzyme of L. acidophilus PF01 exhibited hydrolase activity against tauroconjugated bile salts, but not glycoconjugated bile salts. It showed the highest activity against taurocholic acid. The maximum BSH activity occurred at approximately $40^{\circ}C$. The enzyme maintained approximately 70% of its maximum activity even at $60^{\circ}C$, whereas its activity rapidly decreased at below $37^{\circ}C$. The optimum pH was 6, and BSH activity was rapidly inactivated below pH 5 and above pH 7.

• 한국수정란이식학회지
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• 제23권3호
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• pp.217-222
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• 2008

In previous studies, we reported that sow which was transferred OPS-freezing embryos not able to deliver a piglet (Kim et al, 2004). This study was conducted to investigate a possibility of gilt as recipients which produce piglets after transfer of OPS-freezing embryos. All transferred embryos were prepared by in vitro production (IVP) system. In vitro culture (IVC) medium used glucose-free NCSU23 supplemented with 5mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at $39^{\circ}C$. From day 3 of IVC, 10% fetal bovine serum albumin was added to the culture medium. In preparing of freezing embryos, embryos were treated with 7.5 $\mu g/ml$ cytochalasin-B for 30 min and centrifuged at $13,000{\times}g$ for 13 min. And then, embryos were exposed sequentially to an ethylene glycol (EG) solution, aspirated into open pulled straw (OPS), and plunged or thawed into the liquid nitrogen. In embryo transfer (ET), we used two kinds of type (surgical method vs. non-surgical method). In surgical method of embryo transfer, $55\sim65$ embryo were transferred in both uterine horn of two recipient gilts by plastic straw. Non-surgical method which is like artificial insemination was performed on three gilts. Each 140 frozen embryos were transferred to two gilts and 40 fresh embryos to one gilt. Pregnancy establishment was shown one recipient at 45 days after ET. However, the one recipient was also aborted at 58 days after ET. These results suggest that gilts can be considered as a candidate of recipients for OPS-freezing embryo transfer.

• 대한수의학회지
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• 제28권1호
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• pp.67-73
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• 1988

This study was conducted to isolate etiological agents from the 103 scouring piglets in Gyeongnam area and also carried out antimicrobial drug susceptibility test and epidemiogical served. The incidence of scouring piglet was most prevalent as 81.6% in the age of 2 to 4 weeks after birth, while the rate was less than 10% in the age of 5 to 6 weeks and under 1 week after birch. When compared the isolation frequency of the each etiological agent, enteropathogenic E. coli was most prevalent as 46.6%, thermophilic Campylobacter 26.2% and Salmonellae was 8.7% in order. In the OK serotyping for 117 isolates of enteropathogenic E. coli, type 0141 : K85 (20.5%), 0157:K88ac(14.5%), 0138:K81 and 0149:K91 (13.3%) were encountered most frequently. In the biotyping for 27 isolates of thermophilic Campylobacter, most strains of C. jejuni were belong to type I (50.0%) and II (25.0%), and most strains of C. coli were belong to biotype I (78.9%). In the serotyping for 9 strains of Salmonellae, 3 strains were grouped as D, 2 strains as C. and each 1 strain was group B and E. The other 2 strains were untypable. The 117 isolates of enteropathogenic E. coli were resistant more than 90% to erythromycin, penicillin, tetracycline and streptomycin, wherease about 90% of the isolates were sensitive to kanamycin and gentamicin. In the case of Salmonellae, all of the isolates were resistant to penicillin, but about 89% of the isolates were sensitive to gentamicin and colistin. All of C. jejuni and C. coli isolates were resistant to cephalothin, but more than 89% of C.jejuni and C. coli were sensitive to kanamycin and gentamicin.

• Asian-Australasian Journal of Animal Sciences
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• 제11권4호
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• pp.381-384
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• 1998

Twenty-three crossbred (Yorkshire $\times$ Duroc $\times$ Hampshire $\times$ Chester White) primiparous sows were used to evaluate the effects of the mineral-salt laxative in lactation diets on sow and litter performance. The sows were fed a sorghum-extruded soybean-based diet with .85% lysine, .90% Ca, .80% P, and 3.2 Mcal ME/kg. Sow body weight (p > .54) and backfat loss (p > .61), average daily feed intake (p > .42), and litter weight gain (p > .74) were not affected by the mineral-salt laxative in the diet. However, survivability of piglets was greater (p < .06) for sows with the mineral-salt laxative in their diet and, thus, number of pigs weaned was increased. As expected, fecal moisture was increased (p < .09) in sows fed the mineral-salt laxative. Apparent digestibilities of DM, N, and GE were not affected by treatment (p > .26). After weaning, stomachs were collected and scored for ulcers and keratinization using a scoring system of 0 = normal to 3 = severe. Severity of ulceration and keratinization was not significantly affected by treatment (row mean scores differ test p > .25), but scores for sows fed the diet containing the mineral-salt laxative were numerically lower than sows fed the control diet. Thus, our data indicate that sows fed the mineral-salt laxative during lactation had improved piglet survivability, greater fecal moisture, and tended to have fewer lesions in the mucosa of the stomach.

• Asian-Australasian Journal of Animal Sciences
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• 제24권6호
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• pp.818-824
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• 2011

Eight LW${\times}$D crossbred, castrated weanling piglets were used to examine the effect of hyperglycemia by streptozotocin (STZ)-injection on oxidative and anti-oxidative status in circulating fluid. Every two of the eight piglets were intravenously administrated STZ at a dose of 0 (control), 100, 125 or 150 mg/kg BW, respectively, and on 15th day after the STZ-injection, some markers of the oxidative stress in circulating fluid were measured to evaluate oxidative and anti-oxidative status in the piglets. First, piglets with hyperglycemia were selected from the STZ-injected piglets as measured by the levels of fasting plasma glucose (FPG) during 2 weeks after the STZ-injection. Additionally, data obtained from the intravenous glucose tolerance test (IVGTT) on 14th day were analyzed. Secondly, the data obtained in this experiment were divided into the control group and the hyperglycemic (STZ) group, and compared. The FPG level or area under curve (AUC) for plasma glucose during the IVGTT in the STZ-induced diabetic piglets was slightly significantly (FPG, p = 0.070; AUC, p = 0.072) higher compared with the control. On the other hand, the plasma level of lipid peroxidation in the STZ-induced diabetic piglets was significantly (p<0.05) higher compared with the control. These results raise the possibility that STZ-induced diabetic piglets produced in this study can be used as a diabetic animal model to research the pathogenic mechanisms or therapy of complications in diabetic mellitus.

• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.568-572
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• 2006

The effect of dietary lysine supplementation on the performance of crossbred lactating sows (n = 49, total 92 litters) and suckling piglets during the warm and cool seasons was studied. Sows were randomly allocated to one of two experimental diets, which were fed throughout a 28-day lactation period. Two diets were formulated based on corn-soybean meal with 6% wheat bran, containing 15% crude protein, and with or without 0.25% lysine-HCl supplementation. No interaction was observed between season and dietary lysine supplementation. Dietary lysine supplementation significantly (p<0.05) increased weaning weight (7.11 vs. 6.46 kg) and daily gain (0.20 vs. 0.18 kg) of piglets. During the warm season lactating sows had significantly lower feed intake (3.78 vs. 6.11 kg, p<0.01), higher weight loss (19.81 vs. 9.73 kg, p<0.01) and backfat loss (0.23 vs. 0.06 cm, p<0.01), longer interval from weaning to estrus (9.32 vs. 6.21 days, p<0.05), lower litter weight gain (42.13 vs. 52.90 kg, p<0.01) and lower daily gain (0.17 vs. 0.21 kg, p<0.01) than lactating sows during the cool season. The results indicate that the influences of dietary treatment and season were independent. The 15.0% CP lactation diet with 0.25% lysine-HCl supplementation did not improve the performance of lactating sows and litter piglets, which was impaired by the warm season.