• Korean Journal of Weed Science
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• v.14 no.1
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• pp.42-48
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• 1994

The toxic compounds of sorghum stem extracts were isolated by different organic solvents and pH, and characterized and quantified in terms of their inhibition of seed germination and seedling growth in Echinochloa colona(L.) Link and radish(Raphanus sativus L.). Sequential partitioning of stem extract with various organic solvents with increasing polarity showed that all fractions of hexane, ethyl ether, methylene chloride, ethyl acetate, and the aqueous remainder inhibited germination and seedling growth in E. colona. Of the five fractions, the ethyl ether fraction had the greatest inhibitory effect on E. colona. Further separation of the ethyl ether fraction at different pH(pH 2-11) showed that phytotoxic compounds were acidic. The result indicates that the phytotoxin present in the stem extract may be nonpolar and acidic.

• Applied Biological Chemistry
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• v.37 no.2
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• pp.115-123
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• 1994

Allelopathic activity of the volatiles from leaves of Perilla frutescens an Artemisia asiatica was determined on the basis of bioassay, which tested germination and seedling growth of radish, rice, mung bean and lettuce. Seedling growth was more inhibited by phytotoxic volatiles than germination. Volatile components collected by headspace cold trapping-Tenax GC adsorption were analyzed by GC-MS. Fifteen volatile components in P. frutescens and 15 components in A. asiatica were identified. By steam distillation-extraction, 4 flavor components in P. frutescens and 10 components in A. asiatica were identified. The inhibitory activity of the fractions, obtained by steam distillation-extraction, was determined by virtue of bioassay on radish. Volatile allelochemicals of the most active fraction, neutral fraction, isolated from P. frutescens contained 9 components. In A. asiatica, 24 volatile allelochemicals were identified.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.24 no.2
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• pp.89-103
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• 1979

The compatibility of herbicide use for soybean cultivation was evaluated from the field and pot trials. Comparing with the check plot, the effects of herbicides at their several doses on the phytotoxic stability of crop plants and on the weeding efficacy were studied. Statistically significant differences were found among the doses, and not among herbicides and interactions between herbicide and dose. Risks on the compatibility for crop plant and weeding efficacy were recognized from the excess-dosed treatments of herbicides tried.

• Korean Journal of Weed Science
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• v.17 no.4
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• pp.407-412
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• 1997

Allelopathic activity of methanol extract from medicinal plants was determined based on the inhibition of germination, shoot and root length of Echinochloa crus-galli. The response was depended on the source of extracts and concentrations. Of the 20 test plants tested, Cnidium officinale showed most inhibition according to germination, shoot and root length of E. crus-galli. Based on the plant parts, the root extract of C. officinale had more inhibitory effect on E. crus-galli than the stem+leaf extract. Phytotoxic effects of the root extract on E. crus-galli showed 75% inhibition of seed germination, 95% inhibition of shoot growth, and 100% inhibition of root growth at the concentration of 1g/petridish. Sequential partitioning of root extract with organic solvents with increasing polarity showed that the ethylacetate fraction had the greatest inhibitory effect on E. crus-galli. Germination was not inhibited at any rate of ethylacetate fraction but shoot and root growth, were significantly inhibited even at 2mg/petridish. This indicates that the most toxic phytotoxin present in the root extract may be moderate polar compound.

• The Korean Journal of Ecology
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• v.28 no.1
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• pp.7-13
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• 2005

In the present study, we investigated the tolerance of Commelina communis to growth in Cu-contaminated soil and water We examined the germination rate, root and shoot growth of seedlings, fresh biomass in soil and water, and ability to eliminate Cu. We found that C. communis eliminated 41% of Cu in soil containing 50 mg Cu/kg and removed over 50% of Cu from water containing 100 mg Cu/L Cu. In addition, the plants could accumulate 90 mg Cu/g when grown in soil containing 50 mg Cu/kg and 140 mg Cu/g when grown in soil containing 100 mg Cu/kg thus higher levels of Cu removal were observed in soils containing higher Cu concentrations. In water, the maximal accumulation rate was 4.9 mg Cu/g root and 1.2 mg Cu/g shoot in water containing 20 mg Cu/L, and 7 days after exposure, Cu absorption saturated. Further, the growth rate of C. communis was not affected by up to 100 mg Cu/kg in the soil. Therefore, the phytotoxic effect of Cu on plants increased as the concentration of Cu was raised, although to different extents depending on whether the Cu was in soil or water. Overall, Cu removal from soil by C. communis was most effective at 100 mg Cu/kg in soil and 10 mg Cu/L in water. Finally, we identified two peaks of Cu-binding ligands in C. communis. Which is a high molecular weight peak (HMWL) at 60 kDa (Fraction 17 to 25) and a Cu binding peptide peak at <1 kDa (Very low molecular weight ligand: VLMWL). Cu-binding peptide (Cu-BP) was observed to have an amino acid composition typical of phytochelations.

• Korean journal of applied entomology
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• v.31 no.2
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• pp.182-189
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• 1992

The influnces of 9 kinds of solvents on the toxicities against several insect species, phytotoxicity and solubility of compounds were evaluated by means of leaf dipping and spray methods. In case of the spray application, density and vapor pressure seemed to be a contributing factor to lethal toxicity against brown planthopper and diamond-back moth, respectively; the bigger the property of density and the smaller the vapor pressure gave the stronger toxicity. It appeared that the toxicity of solvents was not correlated with anyone of physical properties such as boiling point, dipole moment, dielectric constant, surface tension and viscosity. Spray treatment gave more toxicity to insects than leaf dipping treatment. Although dielectric constant and dipole moment seemed to be contributing factors to phytotoxic damage to rice seedling and bean plants, respectively, no general correlation between phytoxicity and the other physical properties was found. Leaf dipping application caused stronger phytotoxicity than spray application. It is concluded that 5% acetone solution may be most suitable to test chemicals because of its favorable solubility of compounds, lower toxicity to insects, and lower phytotoxicity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.2
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• pp.179-184
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• 1981

The study was conducted to know the differential growth responses of soybean cultivars as affected by various application stages of Metribuzin herbicide, at the University experimental farm of Chonnam Nat'l. Univ, Kwangjoo, 1980. Experimented soybean materials were six cultivars, as Kwanggyo, Dongbuktae, Bongui, Yukwoo 3, Suweon 85, and Suweon 86., . and Laid-out application stages were five as pre-sowing, Pre-emergence, Early Post-emergence, and Late Post-emergence treatment comparing with Control. Results obtained from the study were as follows: Among experimented soybean cultivars, no significantly tolerant cultivar was shown. Highly significant differences in phytotoxicities by Metribuzin treatments were detected among various application stages, and crop tolerances were recognized bigger in order as E. Post-emg. < L. Post-emg. $\ll$ Pre-emg. < Pre-sowing $\leq$ Control. The phytotoxic symptoms of all soybean cuitivars were severely recorded in order of Leaf No. /Plant < Plant height $\ll$ Branch No. /Plant. For the cultivation of most soybean cuitivars, the use of Metribuzin in Pre-sowing or Pre-emergence were recommended, and especially it is expected to follow of more detailed study on soil-incorporation method in Pre-sowing treatment, and rather the tank-mixture system of Metribuzin with others than the mono-application system for the safe-use and enlargement of weeding-spectrum.

• Korean Journal of Environmental Agriculture
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• v.7 no.1
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• pp.52-57
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• 1988

This study was carried out to investigate the specific activity fo peroxidase(POD) in conjunction with the phytotoxic effect of the tolerant soybean(Glycine max Merr.) and the susceptible crabgrass(Digitaria adscendens(L.) Scopol.) to acifluorfen. The POD activity increased during the growth of soybean and crabgrass seedlings. But after the foliar application of acifluorfen, the POD activity in the treated leaves was considerably higher than in controlled leaves, suggesting that soybean had higher tolerance to acifluorfen with growth. All the treated soybeans recovered from herbicidal injury in 14 days after treatment, while all the treated crabgrasses were dead with extreme necrosis and leaf burning. It was measured that the POD activity of crabgrass increased abruptly in 1 to 2 days after treatment and then significantly decreased in 5 days.

• The Korean Journal of Pesticide Science
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• v.6 no.3
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• pp.202-208
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• 2002

A phytotoxin was purified by repeated chromatography from liquid cultures of Fusarium oxysporum BG isolated from barnyardgrass. Its chemical structure was determined to be dehydrofusaric acid by mass and NMR spectral analyses. The substance showed a potent phytotoxic activity against growth of duckweed with a $EC_{50}$ value of $1.5{\mu}g/ml$. It also inhibited the root growth of barnyard millet, cress, barnyard grass, and rice cultivar 'Dongjin'. However, it had no inhibitory activity against seed germination of barnyard millet and cress, and the shoot growth of the four plant species.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.225-229
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• 2016

This study was conducted to investigate the herbicidal efficacy of up granule formula of benzobicyclon mixtures, benzobicyclon + azimsulfuron + oxaziclomefone up granule (BAO UG) and benzobicyclon + bensulfuron-methyl + fentrazamide up granule (BBF UG) to control annual and perennial weed species in a rice paddy field. Four annual weed species including Echinochloa crus-galli L. and three perennial weeds were dominated in the experimental field. Application of BAO UG and BBF UG was highly effective to control both annual and perennial weed species. No phytotoxic effect was observed in the rice based on the plant height, the number of tillers, and culm and panicle length. Finally, rice yield in the BAO UG and BBF UG treatment was as much as that in the hand weeding. This study indicates that self-dispersible floating formula of benzobicyclon mixtures can be applied to control both annual and perennial weed species in rice paddy fields and contribute to save labor costs.