• Title/Summary/Keyword: physiological stress

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Physiological Evaluation of Cloned Mini-pigs in a Transportable Isolator for the Study of Xenotransplantation (바이오장기 연구를 위한 이동식 아이솔레이터 내 복제 미니 돼지의 생리 활성 평가)

  • Kim, Hae-Sung;Jeon, Yu-Byeol;Kwak, Seong-Sung;Jeong, Seung-A;Jung, Eui-Man;Hyun, Sang-Hwan;Jeung, Eui-Bae
    • Journal of Embryo Transfer
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    • v.26 no.3
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    • pp.165-169
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    • 2011
  • The present study investigated the physiological evaluation of cloned mini-pigs in a transportable isolator. Transportable isolator was designed and manufactured by our research team for transporting gnotobiotic pig. Until now, no previous reports are available regarding the physiological activities and harmful effects when pigs were transported in this isolator. Five cloned mini-pigs of 1~2 year (s) old female with a body weight between 80~90 kg were used. The effects of transportable isolator on stress-related hormone, adrenocorticotrophic hormone (ACTH) and cortisol levels, and heart rate were evaluated. In addition, it was also examined the effects of transportable isolator on blood chemistry factors (alanine aminotransferase: ALT, aspartate aminotransferase: AST, blood urea nitrogen: BUN, glucose, and creatinine). Blood was sampled just before the beginning of transport (T0), at the end of transport (30min after the transport; T1), and 30 min after the end of transport (T2). At the same time, heart rate was also evaluated. As a result, heart rate had no significant (p>0.05) differences at the various-time points of study (T0, T1, T2). However, heart rate was slightly higher than normal range in T1 and T2. The ACTH level was higher than normal range. Whereas, the cortisol level was lower than normal range. There were no statistical significant differences both ACTH and cortisol level between different time groups. Also, there were no significant differences in blood chemistry factors. Therefore, our present study shows that transportable isolator has no harmful effect on stress and physiological condition in cloned mini-pigs.

Effects of Aromatherapy on Stress, Sleep, Nausea and Vomiting during Patient Controlled Analgesia Treatment of Patients with Hysterectomy (자가통증조절장치 (PCA) 사용자에서 향기흡입법이 자궁적출술 후 스트레스, 수면 및 오심과 구토에 미치는 효과)

  • Choi, JungHee;Kim, Yun Mi
    • Women's Health Nursing
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    • v.19 no.4
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    • pp.211-218
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    • 2013
  • Purpose: The purpose of study was to examine effects of aromatherapy on stress, sleep, nausea and vomiting of women after laparoscopic hysterectomy. Methods: The participants were 60 women who had laparoscopic hysterectomy: experiment group for aromatherapy (n=30) and control group for routine care (n=30). The experimental group received inhalation aromatherapy for 5 minutes, twice; the first was done right after the operation, the second was at 9 pm before sleep on the same day-while the control group had no inhalation. Data were collected from July to September, 2012 at G hospital. Results: The degree of psychological stress was not significantly different between two groups (t=-1.96, p=.054). Yet, there were significant differences between two groups for degree of physiological stress (t=-3.20, p=.002), the level of cortisol (t=-2.01, p=.049), the score of sleep status (t=2.47, p=.016), the score of sleep satisfaction (t=2.43, p=.018), and the score for nausea and vomiting (t=-2.58, p=.012). Conclusion: Inhalation aromatherapy using the mixed oil of lavender, mandarin, and marjoram was effective in decreasing the level of physiological stress, cortisol, and the score for nausea and vomiting, and also allowed the participants to have a better sleep. Therefore, inhalation aromatherapy could be effective in improving the quality of life of these women during recovery.

Physiological Response to Salinity Stress of Japonica/Indica Lines Tolerant to Salt at Seedling Stage

  • Ko, Jong-Cheol;Lee, Kyu-Seong;Kim, Ki-Young;Choi, Weon-Young;Kim, Bo-Kyung;Shin, Woon-Cheol;Ko, Jae-Kwon;Yum, Song-Joong
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.391-398
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    • 2011
  • Physiological responses to salinity stress were evaluated in six rice genotypes differing in their tolerance to salinity at the seedling stage. Susceptible genotypes ('Dongjingbyeo', 'Hwayeongbyeo', and 'IR29') showed salt injury symptoms (mean 8.8) and higher visual score under salt stress than that of tolerant ones ('Pokkali', 'IR74009', and 'IR73571'). As salinity affects growth and physiological parameters, the six genotypes thus showed significant reduction because of salt stress. Tolerant Japonica/Indica bred lines ('IR74009', 'IR73571') showed lower reduction, 33.9%, 34.5%, and 50%, respectively, in plant seedling height, dry shoot weight and dry root weight than those of the susceptible Japonica varieties ('Dongjingbyeo', 'Hwayeongbyeo'), and the highest reduction under salt stress was observed in dry root weight, followed by dry shoot weight and seedling height, respectively. Shoot $Na^+$ concentration of IR74099 and IR73571 was lower than that of the susceptible varieties, 'Dongjinbyeo' and 'Hwayeongbyeo'. There were no significant differences among genotypes in root $Na^+$ concentration. Shoot $K^+$ concentration showed a reverse tendency compared to shoot $Na^+$ concentration. IR74009 and IR73571 had considerably lower ratio compared to 'Dongjinbyeo' and 'Hwayeongbyeo' in $Na^+/K^+$ ratio of their shoot and was not different the tolerant check, 'Pokkali'.

Physiological and Histological Changes of Overfeeding-induced Obese Rainbow Trout Oncorhynchus mykiss (사료 과다공급으로 유도된 비만 무지개송어(Oncorhynchus mykiss)의 생리·조직학적 변화)

  • Park, Jiyeon;Roh, Heyong Jin;Park, Junewoo;Jeong, Dahye;Lee, Mu Kun;Kim, Do-Hyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.5
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    • pp.688-696
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    • 2022
  • Obesity could cause immune-physiological disorders in fish. Yet, little is known about the impact of obesity on stress and histological responses. This study aimed to determine histological and physiological changes in and vulnerability of overfeeding-induced obese rainbow trout Oncorhynchus mykiss exposed to stress condition. Control, intermediate and overfed groups were fed at 1.5, 2.5 and 3.09% of their body weight per day, respectively, for eight weeks. Weight gain, body mass index, hepatosomatic index and serological parameters, and histology of liver were measured in five fish from each group at week 0, 2, 4, and 8. At week 8, 20 fish from each group were exposed to heat stress by increasing water temperature at a rate of 3℃ per day from 15 to 25℃ and maintaining the final temperature for 10 days. Overall, overfed fish showed significantly higher weight gain, body mass index, and serological parameters than those of fish in the other groups. Fish in the overfed and intermediate groups displayed multifocal infiltration of inflammatory cells in hepatic parenchyma. Mortality rate and serological parameters of fish in the overfed group exposed to heat stress were significantly higher than those of fish in the other groups, indicating increased vulnerability to environmental stress.

Effect of Samulanshintang(SA) on Stressed Rats (사물안신탕(四物安神湯)이 스트레스에 미치는 영향)

  • Lim, Seok-rhin
    • Journal of Haehwa Medicine
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    • v.10 no.1
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    • pp.489-494
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    • 2001
  • To determine the preventive effect of Samulanshintang (SA) on stress, we investigated the physiological change of rats which were applied immobilization stress. For immobilization stress, rats were placed in restrainer for 12 hours a day for 3 days. During application of stress, body weight of rats was measured. After sacrifice, 4 organs were taken for measurement of organ weight. Brain was homogenated and its catecholamine and serotonin contents were measured with HPLC. In our study, stress mainly induced increase of concentration of neurotransmitters in brain without other significant physical change of rats. SA inhibited stress induced changes of neurotransmitter content in brain.

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Effects of Kudzu Leaf Extracts on Stress Reduction in Rats with Damaged Larynxes (후두 손상 유발시킨 랫드에 칡잎추출물을 투여하여 스트레스 경감효과에 미치는 영향)

  • Lee, Tae-Jong;Yea, Chun-Jung
    • Journal of Environmental Health Sciences
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    • v.38 no.5
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    • pp.431-437
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    • 2012
  • Objectives: This study aims to investigate the effects of voice disorders on changes in stress among people with damaged larynxes. To accomplish this, physiological changes and reductions in the stress of Sprague-Dawley rats whose larynx had been damaged were investigated after the laboratory animals were administered kudzu leaf extracts with sedative effects. Methods: In the experiment, a total of 24 rats were divided into four groups of six rats, including the normal group, control group, experimental group 1, and experimental group 2. After orally administering to the subjects a predetermined amount of the extract at a specific time (once per day over five weeks), changes in physiological functions, internal organ weight, cortisol, estrogen, and progesterone in the subjects were examined, and an immunological test was conducted on their brain tissues. Results: Statistical significance was seen in the experimental group as opposed to the control group and the results were similar to those of the normal group. Conclusions: In consideration of these results, it is deemed that there are severe effects on stress due to voice disorders and that the administration of kudzu leaf extracts results in improvement in stress.

Effect of Vibration Stress on the Oxygen Consumption, Ammonia Excretion and Blood Characteristics of the Cultured Eel, Anguilla japonica (뱀장어, Anguilla japonica의 산소소비, 암모니아 배설 및 혈액성상에 미치는 진동의 영향)

  • 이정열;허준욱
    • Journal of Aquaculture
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    • v.17 no.4
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    • pp.262-267
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    • 2004
  • Physiological responses (oxygen consumption, ammonia excretion, hemoglobin, red blood cell and white blood cell) of cultured eel, Anguilla japonica to vibration stress were studied in an indoor experimental system. Vibration of 76-93 dB (V) from an electric vibrator was provided in 15-minute intervals during daytime (0800-1800) over a ten day period. Oxygen consumption before the beginning of the experiment (0 day) was 83.9 mg $O_2$$.$kg$^{-1}$ ㆍhr$^{-1}$ . After 1, 5 and 10 days of stress respiration rate decreased by 37.5, 53.7 and 70.5%, respectively. Ammonia excretion showed a similar pattern to that of oxygen consumption. Ammonia excretion decreased by 80.1 % following 10 days of vibration stress. Blood hemoglobin concentration also decreased at 1, 3 and 10th day were 29.4% on day 1,83.9% on day 3 and 87.9% by day 10, while red blood cell counts at day 1 and day 10th were 59.8% and 84.7% lower than initial counts, respectively. The white blood cell count increased by 191.2% at day 7, dropping to 41.5% at day 10. Physiological activity was reduced by 50% following 3.4 days of vibration stress.

A study of Stress Reaction, Symptoms of Stress, Health Promoting Behavior, and Quality of Life in Korean Immigrant Middle Aged Women (미국 이민 중년여성의 스트레스 반응, 스트레스 증상, 건강증진 행위, 삶의 질에 관한 연구)

  • 한금선
    • Journal of Korean Academy of Nursing
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    • v.30 no.3
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    • pp.606-618
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    • 2000
  • The purpose of this study was to identify the level of Symptoms of Stress, Stress Reaction, Health Promoting Behavior, and Quality of Life in Korean Immigrant Middle Aged Women. The subjects of this study were 33 middle aged women who live in Seattle, Washington, U.S.A. Data collection was performed at the U.W from Oct. 1998 to May. 1999. Data collection time was one hour and data was collected through 4 types of questionnaires : SOS, Health Promoting Behaviors, Quality of Life and Demographic data form, and the Physiologic Stress Profile was collected by J&J I-410 biofeedback equipment. The data was analyzed by descriptive statistics and the pearson correlation coefficient using the SAS program. The results of this study are as follows: 1. The level of physiological stress reaction and stress symptoms showed high level and quality of life showed low in general. 2. The Stress Reaction and Symptoms of Stress showed significant negative correlation with health promoting behavior, quality of life in the middle aged women. 3. The health promoting behavior showed significant positive correlation with quality of life in the middle aged women. In conclusion, the physiological stress reaction, symptoms of stress, and health promoting behavior were major influencing factor to quality of life in Korean Immigrant Women. From the results of the study, the following recommendations are presented as follow: 1. It is suggested that the study for developing the health promotion program focused on stress self-regulation for Korean immigrant women. 2. It is suggested that the comparative study for Korean immigrant women and Women in Korea. 3. It is necessary to broaden the scope of nursing practice for middle aged healthy women, so nurses can include a health promotion program focused on stress self-regulating as part of nursing care.

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Comparison of Lifting and Lowering Activity based on Biomechanical, Physiological, Psychophysical Criteria (들기 작업과 내리기 작업의 생체역학적, 생리학적, 정신물리학적 기준치에 의한 비교)

  • Kim, Hong-Ki
    • Journal of the Ergonomics Society of Korea
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    • v.29 no.1
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    • pp.145-153
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    • 2010
  • Activity of lifting has been a major issue in many research area related in manual materials handling tasks. However, the opposite activity of lifting, lowering, has received much less attention. It is known that 52% of all box-handling tasks were lowering in nature. The difference in stress between lifting and lowering activity is not well understood. A simple assumption that these two activities are very similar has been established and widely used. However, this simple assumption may be questionable. The objective of this study was to compare a lifting activity and a lowering activity based on the three different ergonomic approaches; (1) biomechanical, (2) physiological, (3) psychophysical approach. It was found that the stress of lowering activity was from 65% to 93%, from 87% to 97%, and from 87% to 96% according to the biomechanical, physiological, and psychophysical point of view, respectively. It is concluded from the result of this study that the stress of lowering activity is lower than that of the lifting activity. The maximum compressive force on the lumbro-sacral joint (L5/S1) was 158% and 108% respectively, for lifting and lowering activity of which the work load is the 58% of Action Limit. It is suggested that the NIOSH AL and RWL and biomechanical criteria should be reconsidered especially for the low frequency of lifting activities.

Water Physiology of Panax ginseng III. Soil moisture, physiological disorder, diseases, insects and quality (인삼의 수분생리 III. 토양수분, 생리장해, 병해충과 품질)

  • Park, Hoon
    • Journal of Ginseng Research
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    • v.6 no.2
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    • pp.168-203
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    • 1982
  • Effects of soil moisture on growth of Panax ginseng, of various factors on soil moisture, and of moisture on nutrition, quality, physiological disorder, diseases and insect damage were reviewed. Optimum soil moisture was 32% of field capacity with sand during seed dehiscence, and 55-65% for plant growth in the fields. Optimum soil moisture content for growth was higher for aerial part than for root and higher for width than for length. Soil factors for high yield in ginseng fields appeared to be organic matter, silt, clay, agreggation, and porosity that contributed more to water holding capacity than rain fall did, and to drainage. Most practices for field preparation aimed to control soil moisture rather than nutrients and pathogens. Light intensity was a primary factor affecting soil moisture content through evaporation. Straw mulching was best for the increase of soil moisture especially in rear side of bed. Translocation to aerial part was inhibited by water stress in order of Mg, p, Ca, N an Mn while accelerated in order of Fe, Zn and K. Most physiological disorders(leaf yellowing, early leaf fall, papery leaf spot, root reddening, root scab, root cracking, root dormancy) and quality factors were mainly related to water stress. Most critical diseases were due to stress, excess and variation of soil water, and heavy rain fall. The role of water should be studied in multidiciplinary, especially in physiology and pathology.

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