• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.2
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• pp.54-58
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• 2022

This study aimed to investigate the anti-stress function of Aucklandiae Radix (AR) and Cyperi Rhizoma (CR). The essential oils used in the experiment were extracted from AR and CR using Steam Distillation Extraction and Super critical CO₂ extraction. To observe the effects of sample administration, we measured feed intake, leukocytes, red blood cells, hemoglobin, platelets, serum serotonin content, immobility time, climbing time, and swimming time in mice subjected to chronic restraint stress as behavioral changes. The average body weight of all experimental groups increased than the average body weight of the control group. The immobility and climbing times of experimental groups A and B administered with supercritical extraction samples were shorter than those of the other experimental groups and the control group, and the swimming time was longer. The serotonin content in the blood of all experimental groups decreased compared to the normal group, and the serotonin content of the control group was increased. The authors suggest that Korean herbal medicines AR and CR may be utilized as anti-stress flavoring agents based on the above results.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.341-341
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• 2017

Abiotic stress adversely affects crop growth worldwide. Drought of the major abiotic stresses have the most significant impact on all of the crop. The main objective of this study was to assess the effects of drought stress on photochemical performance and vitality of maize (Zea mays L.). The photochemical characteristics were analyzed in the context of period of drought stress during the maize growth. Drought experiment was carried out for four weeks, thereafter, the drought treated maize was re-watered. The polyphasic OJIP fluorescence transient was used to evaluate the behavior of photosystem II (PSII) and photosystem I (PSI) during the entire experiment period. In drought stress, the performance Index (PI) level was reached earlier when compared to the controls. For the screening of drought stress tolerance the drought factor index (DFI) of each variety was calculated as follow DFI= log(A) + 2log(B). All the fourteen cultivars show DFI ranged from -0.69 to 0.30, meaning less useful in selection of drought tolerant cultivars. PI and electron transport flux values of fourteen cultivars were to indicate reduction of photosynthetic performance during the early vegetative stage under drought stress. In conclusion, DFI and energy flux parameters can be used as photochemical and physiological index.

• Journal of Korean Academy of Nursing
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• v.22 no.2
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• pp.238-247
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• 1992

Immigration of Koreans to the United States has increased since the 1960's. Adjusting to life in the United States produces a great deal of stress for immigrants. Despite better economic opportunites, many see the U.S. culture as threatening to their family and cultural values. Differences in culture, language, expectations and social behavior can lead to misunderstandings between health care providers and clients. These misunderstanding can leaf to frustration on the part of each. The ultimate result of this is that often Korean-immigrants do not get their health care needs met and stress response symptoms can lead to disease if there is no appropriate care. To determine the health care needs and concerns of Korean-Americans, a health needs assessment is needed. Appropriate and adequate information about the health care needs of these individuals is important as it relates to American policy changes allowing greater numbers of immigrants to enter the U.S. The purpose of this prospective study was to describe Korean-American stress response. This study focused on the primary presenting problems for which subjects reported having sought care. These included a variety of stress-related symptoms, including peripheral manifestations, cardiopulmonary symptoms, central-neurological symptoms, gastrointestinal symptoms, muscle tension, habitual patterns, depression, anxiety, emotional irritability and cognitive disorganization. Of the 300 subjects who entered the study, 80% (N＝223) completed the questionnaire in full. Demographically, the percentage of females and males was 50% each and they ranged in age from 20 to 69 years. Ninety percent of the subjects were highly educated, 25% owners of business, 25% white collar professionals, 15% employed in sales or as skilled /unskilled labor, 30% had no occupation : and 5% were housewives or students. The SOS inventory is designed to quantify self-perception of behavioral, cognitive, and physiological components of the stress response. It consisted of 94 items divided into 10 subscales. The result of this study are as follows : The total mean 505 of all subjects (N＝223 was .8129 ; the mean 505 for male(N＝114) was .7665 and for females, (N＝108) .8594. The level of symptoms for central-neurologic and muscle tension was higher for than for males. The highest stress response of all subjects was emotional irritability symptoms(1.0644) : the lowest stress response of all subjects was peripheral manifestation symptoms.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.677-686
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• 2013

Edible canna is a productive starch source in some tropical and semitropical regions. In these regions, water deficit stress is one of factors that limit the crop yield. In the present study, we investigated seven physiological indexes and photosynthetic responses of three edible canna (Canna edulis Ker.) cultivars ('PLRF', 'Xingyu-1', and 'Xingyu-2') under 35 days drought stress. Our results indicated that drought treatment caused visible wilting symptoms in all cultivars, especially in 'Xingyu-1'. Coupled with the increase of wilting symptoms, relative water content (RWC) and chlorophyll content decreased progressively, malondialdehyde (MDA) content gradually increased, and key antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities increased first and then decreased in all three cultivars. The effect of water stress was more pronounced in 'Xingyu-1' than in 'PLRF' and 'Xingyu-2', and in lower leaves than in upper leaves. In addition, 35 days drought stress also significantly reduced the photosynthetic capacity. Consistent with antioxidant parameters, photosynthetic changes of 'Xingyu-2' were less than those of the other cultivars under water deficit stress. Drought stress caused a significant increase of water use efficiency (WUE) in 'Xingyu-2', but little in 'PLRF', and obvious decrease in 'Xingyu-1'. These results indicated that 'Xingyu-2' was more tolerant to drought stress than 'PLRF' and 'Xingyu-1' by maintaining lower lipid peroxidation and higher antioxidant enzyme activities.

• Safety and Health at Work
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• v.2 no.2
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• pp.169-175
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• 2011

Objectives: The questionnaire of occupational stress index (OSI) has been popular in the workplace, and it has been tailored for bus drivers in Taiwan. Nevertheless, its outcomes for participants are based on self-evaluations, thus validation by their physiological stress biomarker is warranted and this is the main goal of this study. Methods: A cross-sectional study of sixty-three city bus drivers and fifty-four supporting staffs for comparison was conducted. Questionnaire surveys, 24-hour urine cortisol testing, and blood draws for dehydroepiandrosterone-sulfate (DHEA-S) testing were performed. The measured concentrations of these biological measures were logarithmically transformed before the statistical analysis where various scores of stressor factors, moderators, and stress effects of each OSI domain were analyzed by applying multiple linear regression models. Results: For drivers, the elevated 24-hour urine cortisol level was associated with a worker's relationship with their supervisor and any life change events in the most recent 3 months. The DHEA-S level was higher in drivers of younger age as well as drivers with more concerns relating to their salary and bonuses. Non-drivers showed no association between any stressor or satisfaction and urine cortisol and blood DHEA-S levels. Conclusion: Measurements of biomarkers may offer additional stress evaluations with OSI questionnaires for bus drivers. Increased DHEA-S and cortisol levels may result from stressors like income security. Prevention efforts towards occupational stress and life events and health promotional efforts for aged driver were important anti-stress remedies.

• Molecules and Cells
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• v.45 no.7
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• pp.454-464
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• 2022

DJ-1 is one of the causative genes of early-onset familial Parkinson's disease (PD). As a result, DJ-1 influences the pathogenesis of sporadic PD. DJ-1 has various physiological functions that converge to control the levels of intracellular reactive oxygen species (ROS). Based on genetic analyses that sought to investigate novel antioxidant DJ-1 downstream genes, pyruvate dehydrogenase (PDH) kinase (PDK) was demonstrated to increase survival rates and decrease dopaminergic (DA) neuron loss in DJ-1 mutant flies under oxidative stress. PDK phosphorylates and inhibits the PDH complex (PDC), subsequently downregulating glucose metabolism in the mitochondria, which is a major source of intracellular ROS. A loss-of-function mutation in PDK was not found to have a significant effect on fly development and reproduction, but severely ameliorated oxidative stress resistance. Thus, PDK plays a critical role in the protection against oxidative stress. Loss of PDH phosphatase (PDP), which dephosphorylates and activates PDH, was also shown to protect DJ-1 mutants from oxidative stress, ultimately supporting our findings. Further genetic analyses suggested that DJ-1 controls PDK expression through hypoxia-inducible factor 1 (HIF-1), a transcriptional regulator of the adaptive response to hypoxia and oxidative stress. Furthermore, CPI-613, an inhibitor of PDH, protected DJ-1 null flies from oxidative stress, suggesting that the genetic and pharmacological inhibition of PDH may be a novel treatment strategy for PD associated with DJ-1 dysfunction.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.1
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• pp.56-61
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• 2023

The germination process is critical for plant growth and development and it is largely affected by environmental stress, especially salinity. Recently, hydrogen sulfide (H₂S) is well known to act as a signaling molecule in a defense mechanism against stress conditions but poorly understood regulating seed germination. In this study, the effects of NaHS (the H₂S donor) pretreatment on various biochemical (hydrogen peroxide (H₂O₂) content and amylase and protease activity) and physiological properties (germination rate) during seed germination of oilseed rape (Brassica napus L. cv. Mosa) were examined under salt stress. The seed germination and seedling growth of oilseed rape were inhibited by NaCl treatment but it was alleviated by NaHS pretreatment. The NaCl treatment increased H₂O₂ content leading to oxidative stress, but NaHS pre-treatments maintained much lower levels of H₂O₂ in germinating seeds under salt stress. Amylase activity, a starch degradation enzyme, significantly increased over 2-fold in control, NaHS pretreatment, and NaHS pretreatment under NaCl during seed germination compared to NaCl treatment. Protease activity was highly induced in NaHS-pretreated seeds compared to NaCl treatment, accompanied by a decrease in protein content. These results indicate that NaHS pretreatment could improve seed germination under salt stress conditions by decreasing H₂O₂ accumulation and activating the degradation of protein and starch to support seedling growth.

• Journal of Korean Academy of Nursing
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• v.28 no.4
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• pp.869-880
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• 1998

In order to investigate of the effects of rapid rotating shift work on physiological stress, the activities of urinary Na$^{+}$, $K^{+}$, Cl$^{[-10]}$ were measured in 14 rotational shift nurses, during day shifts(8AM-4PM, n=4), evening shifts(4PM-l2MN, n=5), and night shifts(12MN-8AM, n=5) in hospital twenty students attending nursing college a used as a control group. Urine specimens were collected in 30 minutes before and after work on the second day of shift work. In day shift nurses, Na$^{+}$ activity was 137mM at 8AM and increased to 206mM at 4PM, whereas $K^{+}$ activity was 42mM at 8AM and no significant change at 4PM. Cl$^{[-10]}$ activity was changed from 234mM to 344mM at 4PM at 8AM. In the evening shift, Na$^{+}$ activity was 117mM at 4PM and 140mM at 12MN, $K^{+}$ activity was 22mM and 32mM, respectively. Cl$^{[-10]}$ activity was 169mM and changed to 270mM. During the night shift, Na$^{+}$ activity was 128mM at 12MN and changed to 161mM at 8AM, $K^{+}$ activity was 42mM at 12MN and 8AM, and Cl$^{[-10]}$ activity was from 303mM and changed to 355mM. In general, the urinary ion activities seemed to increase after work, however there were no significant changes in ion activities except the Na$^{+}$ increase in day shift. The mean of the activities of $K^{+}$ and Cl$^{[-10]}$ before and after work during the day and night shift were significantly higher than those in control group (P＜0.05). $K^{+}$ activities were also higher than that of evening shift(P＜0.05). However, there was no difference in Na$^{+}$ activity among the control group and three shifts. There was a significant relationship among urinary Na$^{+}$, Cl$^{[-10]}$ and $K^{+}$ in the control group and rotating shift nurses except between Na$^{+}$ and $K^{+}$ in shift. The relationship between Na$^{+}$ and Cl$^{[-10]}$ was low in shift work and there was no significant relationship between Na$^{+}$ and $K^{+}$ in shift, suggesting that the active regulation $K^{+}$ and/or Na$^{+}$ in response to stress upon the shift work disruped the ratio of urinary Na$^{+}$ to $K^{+}$ and also lowered the relationship between $K^{+}$ and Cl$^{[-10]}$ . These results suggest that nurses working the day shift were overloaded and under stress, and the night shift interfered with the physiological rhythm of the nurses.red with the physiological rhythm of the nurses.

• Korean journal of applied entomology
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• v.51 no.3
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• pp.223-230
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• 2012

Some high frequency sounds alter physiological processes of the beet armyworm, Spodoptera exigua. This study investigated the effect of ultrasound (${\geq}$ 20 kHz) on larval feeding, pupal development, and adult mating behavior of S. exigua. Ultrasound suppressed feeding behavior of fifth instar larvae, and 30 or 45 kHz treatment inhibited more than 50% of feeding activity. Larvae treated with ultrasound exhibited alterations in major nutrient compositions in the hemolymph plasma. Plasma protein levels decreased with an increase in ultrasound frequency. In contrast, sugar levels increased with an increase in ultrasound frequency. Lipid levels increased with an increase in ultrasound frequency up to 30 kHz and then decreased at treatments > 30 kHz. Hemocytes, the fat body, and epidermis expressed three heat shock proteins and apolipophorin III. Ultrasound treatment markedly inhibited expression of some stress-related genes. Ultrasound treatment also inhibited S. exigua pupal development by extending the pupal developmental period and preventing adult emergence. Last, ultrasound treatment significantly inhibited adult mating behavior, which resulted in a significant decrease in female fecundity. These results show that ultrasound is a physiological stress to S. exigua.

• Journal of Korean Society of Forest Science
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• v.104 no.2
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• pp.277-284
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• 2015

This study aims to evaluate the impact of forest therapy on neuro-cognitive, psychosocial, and physiological aspect of adolescent internet addiction risk group. We have classified potential and high risk user group as internet addiction risk group according to the criteria of Korean Internet Addiction Proneness Scale(K Scale). Based on the results of k-scale from the adolescents in metropolitan area from May to July 2013, 25 people were selected as Internet addiction risk group. We have randomized 13 participants joining forest therapy camp and 12 participants not joining one, and analyzed the change of the two groups with Continuous Performance, Kimberly S. Young, Connor-Davidson Resilience, Relationship Change Scale, heart rate variability and cortisol. Statistically significant changes were observedd in neuro-cognitive, psychosocial, and physiological variables, Through this study, we can consider that the therapy healing may relieve the level of internet addiction and can be an alternative to control emotional stability and impulsive behavior.