• Journal of Korean Biological Nursing Science
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• v.24 no.4
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• pp.227-234
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• 2022

Purpose: Smoking is a way of coping with anxiety and stress. This study aimed to identify the effects of forest-thermal combined therapy on anxiety and depression in smokers who desire to quit smoking. Methods: Thirty participants were included in the study, 15 in the experimental group and 15 in the control group. Those in the experimental group participated in a three-day forest-thermal combined therapy program. The program includes forest walks, meditation and thermal therapy in the charcoal kiln. Results: Before and after the program, physiological indicators such as cortisol, heart rate variability, and serotonin anxiety level using the state-trait anxiety inventory (STAI), and stress level using the psychosocial well-being index (PWI) were measured in both groups. The differences in STAI (p=.012) and PWI (p=.006) scores between the experimental and control groups were statistically significant. However, cortisol, heart rate variability, and serotonin were not significantly different between the two groups after the program. Conclusion: These results show that forest-thermal combination therapy effectively reduces anxiety and stress in smokers. It suggests that forest-thermal therapy can potentially increase smoking cessation rates.

• Food Science of Animal Resources
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• v.43 no.6
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• pp.1044-1054
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• 2023

Growing evidence indicates a crucial role of the gut microbiota in physiological functions. Gut-brain axis imbalance has also been associated with neuropsychiatric and neurodegenerative disorders. Studies have suggested that probiotics regulate the stress response and alleviate mood-related symptoms. In this study, we investigated the effects of the probiotic Lacticaseibacillus rhamnosus IDCC3201 (L3201) on the behavioral response and fecal metabolite content in an unpredictable chronic mild stress (UCMS) mouse model. Our study shows that chronic stress in mice for three weeks resulted in significant changes in behavior, including lower locomotor activity, higher levels of anxiety, and depressive-like symptoms, compared to the control group. Metabolomic analysis demonstrated that disrupted fecal metabolites associated with aminoacyl-tRNA biosynthesis and valine, leucine, and isoleucine biosynthesis by UCMS were restored with the administration of L3201. Oral administration of the L3201 ameliorated the observed changes and improved the behavioral alterations along with fecal metabolites, suggesting that probiotics play a neuroprotective role.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.734-738
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• 2006

The present study was conducted to determine the effect of cold stress on broiler performance and ascites susceptibility. Male chicks were obtained from a commercial strain of broiler breeders. The trial was divided into two treatments (control and cold stress groups). Ascites was induced in broiler chickens in the trial by exposing the chickens to low temperature (Ta) and by supplying a pelleted diet. The two experimental treatments consisted of: 1) Control group, $33.3^{\circ}C$ the $1^{st}$ wk, $30.2^{\circ}C$ the $2^{nd}$ wk, and $27.5^{\circ}C$ the $3^{rd}$ wk. 2) Cold stress group, $29.0^{\circ}C$ the $1^{st}$ wk, $26.4^{\circ}C$ the $2^{nd}$ wk, and $23.1^{\circ}C$ the $3^{rd}$ wk. From the end of the $3^{rd}$ wk all broilers were reared to 6 wk of age at a constant temperature of $21^{\circ}C$. There was significant difference in live BW during wk 1 to 5. The control group was consistently the heaviest; however, at 6 wk of age, both groups weighed the same. Body weight gain up to 3 wk was significantly decreased by cold stress. During wk 3 and 6 the chicks in the cold stress group had greater BW gain compared with the chicks in the control group. There were significant differences in mortality due to ascites between the groups. During wk 3 and 6 the cold stress group exhibited the most ascites mortality (9.52%) when compared with the control group (1.90%). At 5 wk of age cold stress condition caused significant changes in packed cell volume (PCV), hemoglobin (Hb) and red blood cell counts (RBC). Right ventricle weight was significantly heavier in the cold stress group than the control. There were also significant differences in right ventricle/total ventricle (RV/TV) ratios at 5 wk. the right ventricle/total ventricle ratios in the cold stress group was higher (0.25) than the control group (0.20). It was concluded that, fast growth and cold temperatures are the primary triggers for ascites during commercial broiler production.

• Development and Reproduction
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• v.13 no.1
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• pp.43-49
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• 2009

We examined the effects of the vibration stress on cortisol secretion and hematological characteristics in soft-shelled turtle, Pelodiscus sinensis. For the stressed group vibration of $45{\sim}78 dB(V)$ from electric vibrator applied for 30 min with 2-h intervals during daytime ($08:00{\sim}18:00$) up to 28 days. Using the blood samples collected from ten turtles held once a week after vibration stress, we measured hematocrit, hemoglobin, red blood cells, cortisol, glucose, lactic acid, osmolality, $Na^+,\;K^+,\;Cl^－$, aspartate aminotransferase (AST), and alanine aminotransferase (ALT). The results have showed that P. sinensis received vibration stress exhibit the 'typical' stress-induced physiological responses (cortisol, glucose, lactic acid, osmolality, ions, hematocrit and hemoglobin) induced by vibration stress. Our data suggested that chronic vibration stress caused substantial stress in the animal, and in particular, the persisting elevated levels of AST and ALT would be highly correlated with the adverse effects of the stress. The high hematological characteristics during entire experimental period showed that the P. sinensis could not adapt to chronic stimuli provoked by vibration stress.

• Journal of Ecology and Environment
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• v.36 no.4
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• pp.255-265
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• 2013

This study was conducted to determine the extent of drought resistance based on physiological responses of Calystegia soldanella under water deficit. In order to investigate the changes of plant growth, stomatal density, photosynthesis, chlorophyll fluorescence, the contents of chlorophyll and carotenoid, osmolality, total ion contents, the contents of carbohydrate and proline, C. soldanella was grown under well watered and drought stressed conditions for 12 days. In this study, water-deficit resulted in remarkable growth inhibition of C. soldanella. The effect of water-deficit on plant growth was associated with low osmotic potential of soil. On day 12 after drought treatment, dry weight, relative water contents, number and area of leaves and stem length were lower than those of control. The stomatal conductance and net photosynthetic rate were significantly reduced in water stressed plant to regulate inner water contents and $CO_2$ exchange through the stomatal pore. Chlorophyll fluorescence and chlorophyll contents were not different in comparison with the control, indicating that the efficiency of photosystem II was not affected by drought stress. This results could be explained that water-deficit in C. soldanella limits the photosynthetic rate and reduces the plant's ability to convert energy to biomass. A significant increase in total ion contents and osmolality was observed on day 7 and day 12. Accumulation of proline in leaves is associated with the osmotic adjustment in C. soldanella to soil water-deficit. Consequently, this increase in osmolality in water stressed plant can be a result in the increase of ion contents and proline.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.5
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• pp.848-855
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• 2020

Objective: Shearing is one of the practices that is applied periodically to fiber producing animals, which can also alter resistance of animals to high temperatures in especially summer months. This study aimed to investigate effects of shearing on some physiological and hormonal parameters in Akkaraman sheep during summer season. Methods: This study was carried out on 39 non-pregnant Akkaraman ewes (aged 1.5 years at the beginning of experiment). The 39 ewes were chosen randomly from the flock belonging to the Erciyes University and they were assigned to two groups as follows: i) group A (n = 20) designed as the control group, they were shorn and group B (n = 19) designed as the experimental group, they were unshorn. Prior to the shearing (-1 day) and on days 1, 7, 15, 30, 45, 60, 75, and 90 following the shearing, blood samples were taken from the vena jugularis of each sheep. Cortisol, β-endorphin, growth hormone (GH), thyroxine (T₄), triiodothyronine (T₃), and heat shock protein 70 (HSP-70) concentrations were determined using the enzyme immunoassay method. Body weight (BW), rectal temperature (RT), pulse rate (PR), and respiratory rate (RR) of each sheep were recorded at the same time. The data obtained were analyzed using two-way repeated measures analysis of variance. Results: Statistical analysis showed a significant effect of shearing×period interaction (p<0.01) and a significant effect of period (p<0.01) on BW, HSP-70, cortisol, T₄ and RT, PR, GH, β-endorphin, T₃, respectively. Also these analysis showed no significant effect of shearing×period interaction or period on RR. Conclusion: The results showed that the thermoregulation abilities of sheep were affected by shearing treatment and the shorn ewes were less affected by heat stress. In conclusion, based on the data of this study, shearing can be considered as a necessary management practice that requires protection for sheep from the effect of heat stress.

• Journal of Korean Society of Forest Science
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• v.89 no.4
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• pp.513-521
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• 2000

Red alder (Alnus rubra Bong.) seedlings inoculated with Frankia pure cultures were grown in a walk-in growth chamber for sixteen weeks. Half were inoculated with the spores of the ectomycorrhizal fungus Alpova diplophloeus (Zeller & Dodge) Trappe & Smith. The mycorrhizal seedlings were significantly larger than nonmycorrhizal plants in diameter, and nodule and shoot dry weight by 6 to 16% when their heights were very similar. The mycorrhizal effects on water relations of red alder seedlings were explored in a 30 hours water stress. Mycorrhizal and nonmycorrhizal seedlings did not significantly differ in leaf water potentials, $CO_2$ exchange rates or $N_2$-fixation rates during the drought. Our results suggests that A. diplophloeus mycorrhizas increased red alder seedling growth under well-watered conditions but do not affect water relations of the plant under water-stress.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.7
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• pp.859-865
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• 2011

A bio-check unit and health index were developed to provide information on personal health state with easily available noninvasive measurements and surveys. Four health indices were defined such as cardiovascular index, stress index, obesity index, and management index. Methods were developed to calculate health index scores from measured physiological signals and answer of survey questions. In order to evaluate effectiveness of the health indices, a clinical trial was conducted for 362 persons who visited general hospital for annual health inspection. The cardiovascular index showed a good correlation coefficient of 0.685 with the cardiovascular health graded by a medical doctor. The stress index showed a good correlation coefficient of 0.638 with the results of stress questionnaires being used in the public health center. Once the health index function is added in the bio-check unit, the unit may provide useful contents for personal health management.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.282-287
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• 1999

The object of this study was to determine the difference of the time course changes of transpiration, diffusion resistance and photosynthetic rate of rice at several different growth stages subjected to soil moisture stress (SMS) and recovery by irrigation. A japonica rice cultivar 'Dongjinbyeo', was grown under flooded condition in a plastic container filled with silty loam soil. At 5 main growth stages, the container was treated by SMS until initial wilting point (IWP) and then reirrigated. The duration of SMS until IWP were the longest, 13 days for tillering stage, and the shortest, 7 days for panicle initiation and meiosis stage. The transpiration rate rapidly decreased during SMS and the transpiration rate at IWP of the stressed plant showed 10∼20% compared with control, and the transpiration rate of stressed plant at most growth stages also recovered rapidly after irrigation and then reached 100% of control within a week. The shoot photosynthetic rate in all growth stages rapidly decreased by SMS, and the rates at IWP of stressed plants were de-creased nearly to 0%, beside the treatment at tillering stage. The recovery degree of photosynthetic rate by irrigation ranged from 20 to 90%, showed higher at early growth stages of SMS treatment than that of later stages. At all growth stages the leaf diffusion resistance of stressed plants was over 3 times that of the control resulting from a rapid increase at 3 to 5 days after draining for SMS, and showed quick recovery by irrigation within 3 days after drainage. The above physiological parameters changed in close relation with the decrease of the soil matric potential after SMS. These results indicate that at all main growth stages of rice plants the transpiration and photosynthesis reduction by stomatal closure reponded sensitively to the first stage of SMS closely related with decrease of soil water potential, while those recovery pattern and recovered degree by irrigation are little different by growth stage of rice.

• Journal of Aquaculture
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• v.12 no.3
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• pp.221-228
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• 1999

The effects of sparp changes of water temperature (WT) on the stree response and physiological change of the cultured olive flounder (Paralichthys olivaceus) were examined by manipulating WT (3 patterns) in a running seawater culture system. In the first group (Exp. I), the WT was decreased from 18$^{\circ}C$ to 11$^{\circ}C$ within 6 hours and increased back to the original WT quickly. WT was decreased from 2$0^{\circ}C$ to 11$^{\circ}C$ within 5 hours and main-tained at 11$^{\circ}C$ for 10 hours. and then increased to 2$0^{\circ}C$ in the second group (Exp. II). In the third group(Exp. III) WT was decreased to 11$^{\circ}C$ within 5 hours (type A) or 10 hrs. (type B). In Exp. I and III, the level of serum cortisol was increased from 2.5$\pm$0.3 ng/ml and 2.6$\pm$0.9 ng/ml to 13.6$\pm$3.0 ng/ml and 12.4$\pm$3.2ng/ml, respectively, with WT decrease. However, no consistent tendency in the change of serum glucose level was shown according to WT decrease. In Exp. III, the glucose level of fish in type A was decreased until 5 hours and increased at 7 hours, then decreased until 12 hours where as the glucose level in type B was decreased until 5 hours and stayed at the level of 15.7 mg/dl. The serum osmolality was reduced with WT decrease and the response of serum electrolytes in this experiment conflicted, and a tendency in total protein, AST and ALT was not found following WT decrease. In conclusion, olive flounder responded to the stress caused by WT decrease and acclimated to this condition when the lower temperature was maintained. But there was no stress response in the blood of olive flounder when WT was increased. On the other hand, the degree of stress response in olive flounder was various according to the range and gradient of WT change.