• Korean Journal of Veterinary Research
• /
• v.29 no.3
• /
• pp.245-251
• /
• 1989

In order to manifest the presence of Na-K pump and its property on the unfertilized egg membranes of mouse, membrane potential was recorded under the physiological condition (at $37^{\circ}C$ and 4mM $Ca^{2+}$). After an induction of superovulation, the fresh eggs with zona pellucida were collected from mouse oviduct. Transient hyperpolarization as pump action was recorded after the switch into the high potassium perfusate (15mM $K^+$) from K-free perfusate, and the difference between membrane potential observed just before the perfusion of high potassium solution and the maximal membrane potenlial during the perfusion of high potassium solution was regard as pump activities. The results observed were as follows, 1. Resting mombrane potential was depolarized under the treatment of $10^{-5}M$ ouabain. 2. Pump activities of the unfertilized mouse eggs were $-3.38{\pm}0.61mV$ ($Mean{\pm}SD$, n=6), recorded as transient hyperpolarization due to the electrogenic property. 3. Pump activities were blocked by both treatment of $10^{-5}M$ ouabain and perfusion of Nafree solution, while increased by high $Na^+$ (300mM) perfusion ($-7.45{\pm}0.75mV$, n =2). 4. Hyperpolarization due to pump activity was not altered by $Mn^{2+}$. 5. Above results confirm the presence of ouabain-sensitive Na-K pump, which affected the membrane potential directly, on the unfertilized egg membranes of mouse.

• The Journal of Korean Medicine
• /
• v.26 no.4
• /
• pp.162-167
• /
• 2005

According to the principle of ascending water and descending fire, water has the property of wetting downward, which is the opposite of fire, which has the property of blazing upward. Thus, they work differently according to their innate properties. Nature and the human body maintain harmony through the interaction of ascending water and descending fire. When applied to the human body, the heart and kidney are the center of this principle. In other words, the heart above is the fire and the kidney downward is water. When the heart-fire harmonizes downward, the kidney becomes warm, enabling genuine vital functions to be active. When the kidney yin moves upward, the heart receives the nourishing yin to harmonize nutrients and blood. Thereby, physiological functions become normal throughout the blood meridians. However, in the ascending kidneywater and descending heart-yang of the heart and the kidney, the liver and lung are the major functional organs. In other words, the liver through the dispersing and raising yang functions moves water, which is the vital essence of the kidney, upward. And the lung, through the astriction?clearing of the lung and descending Qi?dispersing functions, moves the heart-fire downward. These functions are deeply related with changing seasons; thus, these functions can be explained with the ascending kidney-water and descending heart-yang of the five viscera.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.3
• /
• pp.479-482
• /
• 2002

Jookrhyuk (Bambusae Caulis in Liqamen) is one of the most important ingredients for treating Diabetes mellitus. Various aspects of quality of Jookrhyuk produced in Korea depending on carbonization process were compared. The pH Value was the highest in Jinyoungsangsa Jookrhyuk(pH 3.17) among the three different Jookrhyuk samples. Damyangsan Jookrhyuk that contains high level of total sugars(8.0 Brix, %) show incresed level of tar contents, it exhibited positive realationship between total sugar and tar contents. Concentration of methanol and phenolic compounds which are related to toxicity were the highest in Damyangsan Jookrhyuk(1.34㎎/㎖, 3.84㎎/㎖ respectively). The harmful heavy elements were not found in the evaluated Jookrhyuk samples. In coclusion, it was suggested that Bambusae Caulis in Liqamen should be further puryfied for using as main oral medicines for treating Diabetes mellitus.

• KSBB Journal
• /
• v.16 no.4
• /
• pp.370-375
• /
• 2001

For the production of new exo-biopolymers from microorganisms, an exe-biopolymer producing bacterial strain was isolated from the composter used in composting of organic wastes. Bacteriological properties of this strain and physicochemical properties of producing exo-biopolymer were investigated. The isolated strain was identified as Enterobacter sp. through its morphological, cultural and physiological characteristics. The results of color reactions, CPC (cetyl pyridinium chloride) precipitation and infra red absorption spectral analysis indicated that this exo-biopolymer was presumed as an acidic polysaccharide with uronic acid. This polysaccharide was identified as hetero-polysaccharide consisting of galactose, mannose and galacturonic acid by gas chromatography, and the molecular weight of exopolysaccharide purified by gel chromatography were about 370,000 daltons. The polysaccharide solutions(0.50-2.0%, w/v) exhibited non-Newtonian flow behavior with pseudoplastic property and showed the ability of gel formation at above 1.5% (w/v) of polysaccharide concentration.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.5
• /
• pp.932-937
• /
• 2002

The purpose of this research was to investigate the effect of Yonggak-san (YGS) on the immune reaction and apoptosis of leukemia cells. Administration of YGS(500 mg/kg) enhanced proliferation of splenocytes, thymocytes and mesenteric lymph node cells, and also YGS accelerated subpopulation of splenic Band T, thymic T and mesenteric lymph node-T lymphocytes, especially significantly increased CD4+-TH cells in BALB/c mice. YGS accelerated phagocytic activity and production of nitric oxide in peritoneal macrophages. YGS induced apoptosis of transplanted-L1210 cells in vivo, increased apoptotic cell death of cultured-L1210 and/or Molt4 human leukemia cells, decreased of mitochondrial transmembrane potential of both cells in vitro. These results suggest that YGS have an immune-regulatory effect and anti-cancer property.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.5
• /
• pp.1009-1015
• /
• 2002

The purpose of this research was to investigate the effect of Yonggak-san (YGS) on the anti-allergic reaction in vivo and in vitro. Administration of YGS(500 mg/kg) enhanced hemaggutination(HA)titer against SRBC. On the while, YGS inhibited hyaluronidase activity in vitro and passive cutaneous anaphylaxis reaction, lethal anaphylaxis and mortality induced by compound 48/80 in mice, YGS decreased Arthus reaction, acute hind paw edema induced by histamine. But YGS did not affect delayed type hypersensitivity induced by SRBC. These results suggest that YGS have anti-allergic action

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.25 no.6
• /
• pp.1039-1043
• /
• 2011

The purpose of this study is to investigate the modulatory effect of emodin on hydrogen peroxide production in human blastoma SH-SY5Y cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Emodin significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 5, 10, 25, and 50 uM in SH-SY5Y (P < 0.05) in dose dependent manner. These results suggest that emodin has neuroprotective property related with its inhibition of hydrogen peroxide production in PIC-induced neuronal cells.

• The Journal of Korea CHUNA Manual Medicine
• /
• v.4 no.1
• /
• pp.55-65
• /
• 2003

The purpose of this article was to contribute to the knowledge of physiological and pathological changes of aging skeletal muscles, and of therapic method. By aging there were changes of distribution of muscle fibers, the loss of muscle mass, the loss of the number of muscle fibers, the loss of glycolysis capacity, the decrease of the oxidative enzymes and muscle vascularization in the skeletal muscles. And as a pathological change, the exhaustive maximal exercise increased oxidative stress that led to oxidative damage which were shown to be implicated in promoting aging. The property of intensity and duration exercise is important not only in keeping human health and physical fitness from oxidative stress, but also for the maintenance of well-being and quality of life.

• 순환기질환의공학회:학술대회논문집
• /
• 2002.11a
• /
• pp.3-18
• /
• 2002

Hemorheology plays an important role in atherosclerosis. Hemorheologic properties of blood include whole blood viscosity, plasma viscosity, hemaocrit, RBC deformability and aggregation, and fibrinogen concentration in plasma. Blood flow is determine by three parameters (pressure, lumen diameter, and whole blood viscosity), whole blood viscosity is one of the key physiological variables. However, the significance of whole blood viscosity has not yet not been fully appreciated. Whole blood viscosity has a unique property, non-Newtonian shear-thinning characteristics, which is primarily due to the presence of RBCs. Hence, RBC deformability and aggregation directly affect the magnitude of blood viscosity, and any factors or diseases affecting RBC characteristics influence blood viscosity. Therefore, on can see that whole blood viscosity is the causal mechanism by which traditional risk factors such as hypertension, hyperlipidemia, smoking, exercise, obesity, age, and gender are related to atherogenesis. In this regard, we included whole blood viscosity in the three key determinants of injurious pulsatile flow that results in mechanical injury and protective adaptation in the arterial system. Because whole blood viscosity is a potential predictor of cardiovascular diseases, it should be measured in routine cardiovascular profiles. Incorporating whole blood viscosity measurements into a standard clinical protocol could improve our ability to identify patients at risk for cardiovascular disease and its complications.

• Korean Journal of Food Science and Technology
• /
• v.9 no.4
• /
• pp.295-305
• /
• 1977

The procedures for the Preparation of regulatory proteins of myofibrill were developed and postmortem changes in the regulatory proteins of myofibrill were investigated. Both the physiological property and molecular shape of ${\alpha}-actinin$ from pre-rigor muscle did not differ from those of ${\alpha}-actinin$ from post-rigor muscle. On the other hand, although tropomyosin of myofibril changed negligibly during the post-mortem storage of muscle, troponin of myofibril changed remarkably.