Browse > Article

Effect of Emodin on Hydrogen Peroxide Production in Polyinosinic-Polycytidylic acid-induced Human Neuroblastoma SH-SY5Y Cells  

Lee, Ji-Young (Department of Pathology, College of Oriental Medicine, Kyungwon University)
Kim, Young-Jin (Department of Pathology, College of Oriental Medicine, Kyungwon University)
Park, Wan-Su (Department of Pathology, College of Oriental Medicine, Kyungwon University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.6, 2011 , pp. 1039-1043 More about this Journal
Abstract
The purpose of this study is to investigate the modulatory effect of emodin on hydrogen peroxide production in human blastoma SH-SY5Y cells induced by the synthetic analog of double-stranded RNA [polyinosinic-polycytidylic acid]. Hydrogen peroxide production was measured by dihydrorhodamine 123 (DHR) assay. Emodin significantly inhibited the polyinosinic-polycytidylic acid (PIC)-induced production of hydrogen peroxide for 0.5, 2, 12, 18, and 24 hr incubation at the concentrations of 5, 10, 25, and 50 uM in SH-SY5Y (P < 0.05) in dose dependent manner. These results suggest that emodin has neuroprotective property related with its inhibition of hydrogen peroxide production in PIC-induced neuronal cells.
Keywords
emodin; neuronal cells; dsRNA; hydrogen peroxide; neuroprotective effect;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 송효인, 김광중. 베타아밀로이드로 유도된 신경세포사멸에 대한 지황(地黃) 및 지황식초(地黃食醋)의 보호효과. 동의생리병리학회지 21(1):190-198, 2007.
2 강태훈, 채수림, 최우영, 박 찬, 남재환, 주영란, 박근영, 조해월, 김인범. 생쥐 뇌에서 일본뇌염바이러스 감염에 의한 신경세포 자멸사 연구. Anatomy and Cell Biology. 38(3):259-264, 2005.
3 Richardson, M.P., Ayliffe, M.J., Helbert, M., Davies, E.G. A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitrobluetetrazolium test. J Immunol Methods. 219(1-2):187-193, 1998.   DOI
4 Jirapongsananuruk, O., Malech, H.L., Kuhns, D.B., Niemela, J.E., Brown, M.R., Anderson-Cohen, M., Fleisher, T.A. Diagnostic paradigm for evaluation of male patients with chronic granulomatous disease, based on the dihydrorhodamine 123 assay. J Allergy Clin Immunol. 111(2):374-379, 2003.   DOI   ScienceOn
5 Shankaran, H., Chrisler, W.B., Sontag, R.L., Weber, T.J. Inhibition of ERK oscillations by ionizing radiation and reactive oxygen species. Mol Carcinog. 50(6):424-432, 2011.   DOI   ScienceOn
6 Goodwin, A.C., Murray-Stewart, T.R., Casero, R.A. Jr. A simple assay for mammalian spermine oxidase: a polyamine catabolic enzyme implicated in drug response and disease. Methods Mol Biol. 720: 173-181, 2011.
7 전국한의과대학 공동교재편찬위원회. 본초학. 서울, 영림사, p 583, 584, 1991.
8 전국한의과대학 공동교재편찬위원회. 본초학. 서울, 영림사, p 242, 243, 1991.
9 천연물화학 교재편찬위원회. 천연물화학. 서울, 영림사, p 253, 254, 2004.
10 Sun, B., Lu, C., Zhou, G.P., Xing, C.Y. Suppression of Par-4 Protects Human Renal Proximal Tubule Cells from Apoptosis Induced by Oxidative Stress. Nephron Exp Nephrol. 117(3):e53-e61, 2010.
11 Graber, D.J., Park, P.J., Hickey, W.F., Harris, B.T. Synthetic triterpenoid CDDO derivatives modulate cytoprotective or immunological properties in astrocytes, neurons, and microglia. J Neuroimmune Pharmacol. 6(1):107-120, 2011   DOI   ScienceOn
12 Melillo, A.A., Bakshi, C.S., Melendez, J.A. Francisella tularensis antioxidants harness reactive oxygen species to restrict macrophage signaling and cytokine production. J Biol Chem. 285(36):27553-27560, 2010.   DOI
13 박완수. 黃芩 물추출물이 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향. 대한본초학회지 26(1):53-58, 2011.
14 Feng, Y., Huang, S.L., Dou, W., Zhang, S., Chen, J.H., Shen, Y., Shen, J.H., Leng, Y. Emodin, a natural product, selectively inhibits 11beta-hydroxysteroid dehydrogenase type 1 and ameliorates metabolic disorder in diet-induced obese mice. Br J Pharmacol. 161(1):113-126, 2010.   DOI   ScienceOn
15 Feng, Y., Huang, S.L., Dou, W., Zhang, S., Chen, J.H., Shen, Y., Shen, J.H., Leng, Y. Emodin, a natural product, selectively inhibits 11beta-hydroxysteroid dehydrogenase type 1 and ameliorates metabolic disorder in diet-induced obese mice. Br J Pharmacol. 161(1):113-126, 2010.   DOI   ScienceOn
16 Fila, L., Musil, J. Examination of exhaled breath condensate in cystic fibrosis. Cas Lek Cesk. 149(4):173-177, 2010.
17 박완수. 白蔘과 紅蔘이 포함된 理中湯의 마우스 대식세포 내 hydrogen peroxide 생성에 미치는 영향. 동의생리병리학회지 25(1):78-83, 2011.
18 이지영, 박완수. 紅蔘理中湯이 LPS로 유발된 마우스 대식세포 RAW 264.7의 nitric oxide 및 hydrogen peroxide 생성에 미치는 영향. 동의생리병리학회지 25(2):294-299, 2011.
19 이지영, 박완수. 白蔘이 포함된 理中湯약침액의 LPS로 유발된 마우스 대식세포의 nitric oxide 및 hydrogen peroxide 생성에 미치는 영향. 경락경혈학회지 28(1):61-69, 2011.
20 박완수. 黃芩 물추출물이 LPS로 유발된 마우스 대식세포의 hydrogen peroxide 생성에 미치는 영향. 경락경혈학회지 28(3):33-41, 2011.