• The Korean Journal of Physiology
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• v.8 no.2
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• pp.67-74
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• 1974

The object of this research is aimed to determine the activity of adenyl cyclase in both skeletal muscle sarcolemma and fat cell ghost of epididymal adipose tissue isolated from rats exposed to cold for various length of time in an attempt to evaluate whether the tissue sensitivity to catecholamine is increased when rats are exposed to cold for long periods of time Methods: a)Animals: Albino rats ranging in weight from 150 to 200 gm were used throughout this study. For experimental purposes, the rats are divided into two groups: experimental animals were place4 in a cold room at $4^{\circ}C$, controls being kept at $25^{\circ}C$. At the end of 2, 4, 6, 12, and 16 weeks. exposure to cold the rats were used to measure the adenyl cyclase activity. b) Isolation of plasma membrane from skeletal muscle and adipose tissue: The Plasma membrane of skeletal muscle from hind limbs of rats are prepared by the method employed by Rosenthal et at. and fat cell ghost of epididymal adipose tissue of rats by the method employed by Rodbell. c) Adenyl cyclase assay: Adenyl cyclase activity were measured by the method employed by Marinetti et al. Briefly, plasma membrane was incubated with $3^H-ATP$, various amount of noradrenaline and other incubation mixture at $37^{\circ}C$ for 20 minutes. After stopping the enzyme reaction by immersion in boiling water, carrier 3',5'-AMP was added to the system as a marker and $100\;{\mu}1$ aliquots of incubation mixture were pipetted on $20{\time}20$ Whatman No. 3 MM filter paper for one dimensional chromatography. The cyclic AMP spots were cut off and placed in counting vials containing 10ml of Bray's scintillation cocktail. Radioactivity was determined with a Packard Tri-Carb liquid scintillation counter. The enzyme activity is expressed as nanomoles of cyclic AMP produced per mg of membrane per hour. Result: 1. Average adenyl cyclase activity in the plasma membrane of skeletal muscle before and after noradrenaline administration was significantly higher in the cold-exposed rats as compared to the control. Continuous exposure to cold Produced an increased adenyl cyclase activity before and after noradrenaline administration. Adenyl cyclase activity reached peak levels at the 6 weeks exposure to told and level of adenyl cyclase activity remained high. Noradrenaline administration to the incubation medium induced a significant increase in adenyl cyclase activity and the degree of stimulation were proportional to the hormonal concentration But the rate of inclement in adenyl cyclase activity by noradreasline was the same in both groups. 2. Adenyl cyclase activity in fat cell ghost between cold exposed and control rats showed no significant differences before and after noradreualine administration. In summary, it can be concluded that cold adaptation give rise an increased activity of adenyl cyclase in plasma membrane of skeletal muscle in rats.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.136-143
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• 1995

Using a PEG- dextran two phase partition method, plasma and intracellular membrane separated from microsomal membrane of canola (Brassica napus) leaves have been fractionated by centrifugation. $K^{+}$- ATPase specific activity in the plasma membrane (U$_2$ phase) of plants grown at $25^{\circ}C$ and 1$0^{\circ}C$ were 6.6 and 4.6 times, respectively that of the microsomal membrane. Plasma membrane had a lower cytochrome- c- oxidase specific activity than the microsomal membrane or intracellular membrane, while intracellular membrane (L$_2$ phase) had a high cytochrome-c- oxidase but little $K^{+}$- ATPase specific activity. The plasma membrane of canola grown at 1$0^{\circ}C$ had higher 18:3 to 18:2 (linolenic to linoleic acid) ratio (29.2% ) and higher degree of unsaturation than that grown at $25^{\circ}C$ The double bond index of plasma membrane from canola grown at 1$0^{\circ}C$ increased by 8.9% relative to canola grown at $25^{\circ}C$. Similar, intracellular membrane increased by 19.7% at 1$0^{\circ}C$. Canola grown at 1$0^{\circ}C$ was lower in chlorophyll contents (17.3%) than that grown at $25^{\circ}C$. These changes in fatty acid unsaturation were attributable largely to change in Cl8 fatty acid, with major changes occurring in linolenic acid (18 :3) which might have a physiological role of membrane to adaptation on low temperature.ure.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.930-942
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• 2008

Following conclusions were derived from researching various consumption caused after surgical operation. The meaning of surgery in HyungSang medical point of view is a big flaw of the original configuration. Therefore meaning, the loss of container to store something. Changes of the body after surgery can be formation of stagnated blood and abnormal cyst of the removed part and entangled undigested food and stagnant blood. After surgery when the symptoms become chronic or the patients develop side effects as they age, consumption and aging process begins. HyungSang medical treatments of consumption caused after surgery are the followings. We should prevent aging of the framework by using appropriate medication to make up for faults in Gall Bladder, Bladder type and six meridian type by considering its form, color, pulse and symptoms. Stability of emotion that can endure various mental stimulation is needed. Prevent stagnation, indigestion, obstruction and numbness by smoothing up the constructive energy and the defensive energy in the operated part. The Heart and Kidney should be under control and Stomach and Spleen should be reinforced so that food intake is accelerated and produce vital substances and Ki and blood. Eum blood and Yang Ki should circulate around the body well. Examine daily life and if small changes in skin complexion, appetite, sleep and excretion occur, also difference in adaptation of external stimulation and mental health occurs immediate treatment and care is necessary. Treatment of side-effects caused from both aging process and post-surgery in HyungSang's view point is considered better than other ways of treatment and will contribute in expanding the field of medical science.

• Journal of Physiology & Pathology in Korean Medicine
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• v.18 no.2
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• pp.311-343
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• 2004

Various aspect of epidemics broke out continually from the middle of Joseon Dynasty due to the famine and drought caused by abnormal climate of the sixteenth century and the war. Thus the Dynasty performed sacrificial rites, isolated the patients and published plenty of medical books related epidemics in order to cure of the patients, and Heojun edited 『Byeokyeoksinbang』 as 'Dangdokyeok' broke out at Gwanbuk(關北) districts in 1613, Heojun explained the cause of Dangdokyeok as meteorology under the feudal conditions, and concluded Simhwa(心火) by fever toxin, Therefore he selected the method of puting out Simhwa by attack of fever toxin. In addition he presented emergency treatment that can maintain the airway by bleeding. To treat Dangdokyeok, Heojun presented lots of prescriptions so as Seungmagalgeuntang(升麻葛根湯), Cheongyeolhaedoksan(淸熱解毒散), Yeongyopaedok-san(連翹敗毒散), Bangpungtongsaongsan(防風通聖散), Jowiseunggitang(調胃升氣湯) and Hwangryeonhaedoktang(黃連解毒湯) etc. And he proposed Samdueum (三豆飮), Realgar(石雄黃) and so on to prevent infection from that. They presume from 120 to 150 years as the period of human adaptation to the first epidemics. Dangdokyeok put a large number of people to death at first, but it wasn't referred at the history any more after Byeokyeoksinbang. So we can say that the treatment of Heojun may be effective. Common cold and dyspeptic cold broke out in our country differently from 'Shanghan(傷寒)' in the China, so we had settled 'pestilence infectious epidemic disease(瘟疫)' while 'epidemic febrile disease(溫病)' of the China. Dangdokyeok of Heojun is similar to 'Scalet fever' belonging to 'virulent heat pathogen(溫毒)', 'newly epidemic febrile disease(新感溫病)'. As a cure of Dangdokyeok, the Korean medicine uses the treatment of removing fever state whereas the western medicine uses the antibiotics to kill the streptococcus. The symptoms of Dangdokyeok are remarkably similar to those of the Scarlatina, so this occupies a high position on the world history of medicine in aspects of the period and details of symptoms. These days we have the problems that the tolerance of antibiotics increases and disease of unknown cause is prevalent. It means the western medicine get to limits. So if we progress epidemiography based on Heojun's medicine, we may contribute to the world history of medicine.

• Journal of Korean Ophthalmic Optics Society
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• v.12 no.1
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• pp.23-33
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• 2007

It has been reported high concentrations of zinc and taurine in ocular tissue, especially the retina-choroid, and the presence of physiological levels of zinc and taurine in these tissues seem essential for their normal function. In addition, several studies have reported temperature as another effector to the visual sensitivity. But, in spite of many studies, there are still remained many questions about their function and correlation in visual adaptation system. The purpose of present study was to clarify these points using electroretinogram(ERG) recording and absorption spectra scanning, before and after zinc and taurine treatments and hypothermic-effect in bullfrog(Rana catesbeiana) which is one of the poikilothermal animal. The optimal zinc concentration used in this study was determined $10^{-4}M$ while the optimal taurine concentration was 10-5 M, and temperature change for hypothermic-effect went through '$25^{\circ}C {\rightarrow}0^{\circ}C{\rightarrow}25^{\circ}C$'. In ERG recording, it is obtained that dark-adapted threshold became elevated and b-wave amplitudes was increased with zinc and taurine treatment and hypothermic-effect. In absorption spectra scanning, there is distinct absorbance increment over the whole spectral range(400~750 nm) after zinc and taurine treatment and hypothermic-effect. Furthermore there are some synergism effects between zinc and taurine as well as between zinc and hypothermic-effect as a result of co-treatment, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.10
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• pp.1359-1366
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• 2000

An experiment was carried out to study plasma levels of hormones and metabolites of crossbred Holstein cattle during late pregnancy (28 days pre partum), early lactation (30 days post partum), mid-lactation (120 days post partum) and late lactation (210 days post partum). Two breed types of Holstein $Friesian{\times}Red$ Sindhi (50:50 = 50%HF) and Holstein $Friesian{\times}Red$ Sindhi (87.5:12.5 = 87.5%HF) were divided into four groups of four animals each. Two groups of each breed were fed with either rice straw treated with 5% urea or pangola hay (Digitaria decumbens) as the source of roughage throughout the experiments. There were a substantial increases in the mean levels of total triiodothyronine ($T_3$), insulin and glucagon at the onset of lactation, and maintained in a high levels during lactation advance for all groups of experiments. The mean levels of prolactin and thyroxine ($T_4$) were not significantly different among groups of animals, but the plasma cortisol concentration was slightly higher in both groups of 50%HF in comparison with those of 87.5%HF animals. The mean levels of plasma growth hormone (GH) of both groups of 87.5%HF animals feeding on either hay or urea treated rice straw markedly rose in the early period of lactation and markedly reduced in mid- and late lactation. These changes were accompanied with changes of milk yield. In contrast to 50%HF animals, plasma GH levels were considerably higher in the late pregnant period than in the early period of lactation and it remained constant as its value at the early lactation throughout the experimental period. The high levels of both plasma progesterone and estradiol concentration significantly declined after parturition and remained low through lactating period. The plasma glucose level in the 50%HF animals feeding on either hay or urea treated rice straw was higher than the 87.5%HF animals in all periods of experiments. Changes in plasma FFA levels of both types of crossbred animals were depended on the endocrine status during late pregnancy and lactation. The levels of plasma FFA of 50%HF animals were significantly higher (p<0.05) than those of 87.5%HF animals during late pregnancy. Both plasma ${\beta}$-hydroxybutyrate and lactate concentrations were not affected by feeding on either hay or urea treated rice straw during late pregnancy and lactation. These data demonstrate that there were no differences in the physiological performances in the same crossbred animals fed either hay or urea treated rice straw. The 87.5%HF animal has the genetic potential for a high milk yield and homeorhetic adaptation for mammary function differed from 50%HF animals during periods of lactation. Altering lactation persistency in 87.5%HF is regulated mainly by chronically acting growth hormones through the period of lactation.

• Korean Journal of Microbiology
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• v.53 no.3
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• pp.149-155
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• 2017

The nitrogen phosphotransferase (PTS) system is a regulatory cascade present in most Proteobacteria, where it controls different functions. The nitrogen PTS is usually composed of $EI^{Ntr}$ (encoded by the ptsP gene), NPr (encoded by the ptsO gene), and $EIIA^{Ntr}$ (encoded by the ptsN gene). While $EIIA^{Ntr}$ plays a role in a variety of cellular processes, such as potassium homeostasis, regulation of ppGpp accumulation, nitrogen and carbon metabolisms, and regulation of ABC transporters, little information is available for a physiological role of NPr. A recent study showed that dephosphorylated NPr affects adaptation to envelope stresses in Escherichia coli. In this study, we provide another phenotype related to NPr. The ptsP mutant showed a filamentation phenotype. The filamentation phenotype of the ptsP mutant was recovered by additional deletion of the ptsO gene, but not by additional deletion of the ptsN gene, suggesting that an increased level of dephosphorylated NPr in the ptsP mutant renders cells the filamentous growth. This idea was confirmed by the fact that cells with increased levels of dephosphorylated NPr shows the filamentation phenotype. Additionally, we showed that cell size of E. coli increases with incremental dephosphorylated NPr concentrations. These results suggested that dephosphorylated NPr induces morphological change of E. coli.

• Proceedings of the Korean Environmental Health Society Conference
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• 2003.06a
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• pp.157-157
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• 2003

To evaluate an effect of pathological liver damage on the cyclohexane metabolism, rats were pretreated with 50% $CCl_4$ dissolved in olive oil (0.1$\mell$/100g body weight) 10 or 17 times intraperitoneally at intervals of every other day. On the basis of liver function and histological findings, the animals pretreated with $CCl_4$ 10 times were identified as acutely liver damaged ones and the animals pretreated with $CCl_4$ 17 times were identified as severly liver damaged ones, with fibrosis, biliary abnormality and mild injury both in the kidneys and the lungs. To these liver damaged animals, cyclohexane (a single dose of 1.56g/kg body weight, i.p.) was administrated at 48 hours after the last injection of $CCl_4$. The rats were sacrificed at 4 or 8 hours after injection of cyclohexane. The cyclohexane metabolites; cyclohexanol (CH-ol), cyclohexane-1, 2-diol (CH-1, 2-diol), cyclohexane-l, 4-diol (CH-1, 4-diol), and their glucuronyl conjugates and cyclohexanone (CH-one) were detected in the urine of cyclohexane treated rats. After cyclohexane treatment, the serum levels of CH-ol and CH-one were remarkably increased at 4 hours and then decreased at 8 hours in normal group. Whereas in liver damaged rats, these cyclohexane metabolites were higher at 8 hours than at 4 hours. The excretion rate of cyclohexane metabolites from serum into urine was more decreased in liver damaged animals than normal group, with the levels of excretion rate being lower in $CCl_4$ 17 times injected animals than 10 times injected ones. However, it was interesting that the urinary concentration of cyclohexane metabolites was generally more increased in liver damaged animals than normal ones, and the increasing rate was higher in $CCl_4$ 17 times injected rats than 10 times injected ones. And liver damaged rats, especially $CCl_4$ 17 times treated ones, had an enhanced ability of glucuronyl conjugation to cyclohexanol analogues compared with normal group. Futhermore, CH-1, 2 and 1, 4-diol were all conjugated with glucuronic acid in $CCl_4$ 17 times injected animals. In conclusion, the metabolic rate of cyclohexane was unexpectably accelerated and it may be caused by physiological adaptation of adjacent intact hepatocyte in damaged liver.

• The Korean Journal of Ecology
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• v.22 no.3
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• pp.139-144
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• 1999

In order to clarify ecological survival strategy of Rumex crispus community dominating under contaminated area of lower region of Kumho riverside including Chimsangyo (CS), Paldalgyo (PD), Talseochon (TS) and Kumhogyo (KH), we analyzed the content of heavy metals and inorganic matter and vegetative growth. R. crispus showed rapid formation of community by high growth rate, high T/R ratio and showed maximum T/R ratio at the contaminated area Talseochon. Nitrogen and phosphorus contents in R. crispus showed high value in shoot than that of root. T/R ratio of nitrogen and phosphorus showed 3.1∼3.6 and 1.5∼4.5 for the early growth stage, and 6.7∼17.3 and 3.9∼8.3 for the late one, respectively. The absorbed heavy metals by riot were translocated to shoot, the heavy metal content in shoot higher than those in root of Cu, Zn, Fe, and Pb for 3.6, 1.7, 1.5 and 4.8 times, respectively. Distribution ratio of the heavy metals in each organ showed 61∼85% and 15∼39% for shoot and root, respectively. R. crispus accumulated heavy metals in the order of Fe>Zn>Cu>Pb in shoot, and showed maximum values of Cu, Zn, Fe and Pb for 89.7, 376.6, 2946.1 and 13.2 ㎍/g dw, respectively at Talseochon in April. A physiological and morphological characteristics of R. crispus showed thickened leaf, increased water content above 80% and rapid growth of shoot. R. crispus showed ecological adaptation to the contaminated area by transportation of heavy metals and inorganic matter to shoot, and by accumulation of Ca ion in root.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.7-7
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• 2017

Unfavorable weather and soil conditions reduce rice yield and land and water productivity, aggravating existing encounters of poverty and food insecurity. These conditions are foreseen to worsen with climate change and with the unceasing irrational human practices that progressively debilitate productivity despite global appeals for more food. Our understanding of plant responses to abiotic stresses is advancing and is complex, involving numerous critical processes - each controlled by several genetic factors. Knowledge of the physiological and molecular mechanisms involved in signaling, response and adaptation, and in some cases the genes involved, is advancing. Moreover, the genetic diversity being unveiled within cultivated rice and its wild relatives is providing ample resources for trait and gene discovery, and this is being scouted for rice improvement using modern genomics and molecular tools. Development of stress tolerant varieties is now being fast-tracked through the use of DNA markers and advanced breeding strategies. Large numbers of drought, submergence and salt tolerant varieties were commercialized over recent years in South and Southeast Asia and more recently in Africa. These varieties are making significant changes in less favorable areas, transforming lives of smallholder farmers - progress considered incredulous in the past. The stress tolerant varieties are providing assurance to farmers to invest in better management of their crops and the ability to adjust their cropping systems for even higher productivity and more income, sparking changes analogous to that of the first green revolution, which previously benefited only favorable irrigated and rainfed areas. New breeding tools using markers for multiple stresses made it possible to develop more resilient, higher yielding varieties to replace the aging and obsolete varieties still dominating these areas. Varieties with multiple stress tolerances are now becoming available, providing even better security for farmers and lessening their production risks even in areas affected by complex and overlapping stresses. The progress made in these less favorable areas triggered numerous favorable changes at the national and regional levels in several countries in Asia, including adjusting breeding and dissemination strategies to accelerate outreach and enabling changes at higher policy levels, creating a positive environment for faster progress. Exploiting the potential of these less productive areas for food production is inevitable, to meet the escalating global needs for more food and sustained production systems, at times when national resources are shrinking while demand for food is mounting. However, the success in these areas requires concerted efforts to make use of existing genetic resources for crop improvement and establishing effective evaluation networks, seed production systems, and seed delivery systems to ensure faster outreach and transformation.