• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.68-75
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• 1982

This study was carried out to promote percent survival of the walnut seedling grafting. The hardwood scions of the walnut were grafted on the nures seed-stock of the Juglans mandshurica Mat in an electric heating bed, then planted in field. The results obtained from the study were as follows : The optimum time of scion cpllection was from January to February. The best medium of the seed bed was sandy soil. The best grafting time was form the early to the 20the of the march. When the grafted seedling in the heating bed was trans-planted on filed 90percent of the seedlings was survived until autmn. The percent grafting on the elective heating bed was 90%. Crown gall occuring frequently in chestnut nurse graft was not appeared in juglans mandshurica Max grafted seedling of after outplanting. The grafted seedlings have not shown any physiological defects but developed normaly 3 years since grafting.

• Journal of Korean Society of Forest Science
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• v.90 no.3
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• pp.306-313
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• 2001

The objectives of this study were to understand the physiological responses and resistance of red pine trees to ozone exposure in relation to nitrogen and phosphorus fertilization. Potted one-year-old seedlings of Pinus densiflora S. et Z. were exposed in an open-top chamber(OTC) to ozone at concentration of 0.12ppm for 3 hours daily for eight weeks with or without N and P fertilization alone or in combination. The OTC had dimensions of 2.0m in height and 2.5m in diameter, and the air in a control chamber was filtered with activated charcoal to maintain the ozone concentration below 0.02ppm. After eight weeks of ozone exposure, none of the seedlings showed any symptoms of visible injury on leaves. The seedlings fertilized with N and P in a control chamber showed 22 to 95% increase in total dry weight, and similar fertilizer effect was also noticed in an ozone chamber. Ozone treatment did not decrease the total dry weight, but increased shoot/root ratio by 14.5%. Ozone treatment increased sucrose content in the leaves by 23%, but decreased sucrose content in roots by 20% regardless of N or P application. Starch content in the leaves was not affected by either ozone or fertilizer. However, starch content in the roots was decreased by 41% by ozone treatment. Chlorophyll content in the leaves was increased by 70% by N application, but was not affected by ozone treatment. Nitrogen and P fertilization stimulated net photosynthesis by 80% in a control chamber, but stimulatory effect of N and P on net photosynthesis was 22.3% less in an ozone chamber. Net photosynthesis of the seedlings with no fertilization was not affected by ozone treatment. Based on the observed interactions between N, P, and ozone, it was concluded that the stimulatory effect of fertilization on growth of Pinus densiflora would be decreased by ozone treatment, but fertilization would increase resistance to ozone by re-allocation of increased carbohydrates.

• The Korean Journal of Physiology
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• v.3 no.2
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• pp.9-16
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• 1969

It is well known that a number of -SH and -SS containing substances afford a certain measure of protection against radiation effects in many biological systems, and it is conceivable that inherent -SH levels in Ehrlich ascites tumour (ELD)cells may be of decisive improtance with respect to the development of cellular radiation injury. So far, little effort has been directed to elucidate the changes in levels of different -SH and -SS groups in ELD cells when the tumour-bearing whole animal was subjected to the sublethal dose of X-irradiation. The present study was designed to bring some lights in the possible changes of and relationship between various sulfhydryl levels, such as P-SH, NP-SH and NP-SS, as well as the content of protein and cell volume of ELD cells, after subjecting the ELD mice to 1,200 r of X-irradiation. The animals used in this experiment were all mixed bred mice of $20{\sim}25\;gm$ in body weight (approximately 2 months old) irrespective of sex. 12 mice in one experiment were inoculated intraperitoneally with 0.2 ml of ascites tumour cells $(2{\times}10^6\;cells)$, and on the 7th day of the tumour growth, they were X-irradiated with 1,200 r, using the conventional X-ray machine under the following conditions: 200 Kv at 15 mA, 0.5 mm Cu filter, target-skin distance: 50 cm. Radiation dose was measured with the the Philip integrating dosimeter. At 24, 36, 48 and 60 hours after the X-irradiation, the mice were killed by cervical dislocation, and the tumours were taken out. Freshly withdrawn ascites tumours were placed in ice, and immediately the cell concentration was measured with the Coulter Cell Counter (Model B), and the hematocrit of the tumour cells were also determined. Cell volume was thus calculated by the cell concentration and hematocrit value. P-SH content of ELD cells was measured potentiometrically according to the method of Calcutt & Doxey, and NP-SH and NP-SS contents were measured spectrophotometrically by the method described by Ellman. Protein content of ELD cells was determined with the Folin phenol reagent by Lowry et al. Altogether, 48 experimental mice were used, and 12 mice with the only exception of X-irradiation were used as the control. Results obtained indicate that the contents of all the cellular sulfhydryl groups as well as cell volume and protein content of the ELD cells increase significantly as time progresses after the sub-lethal X-ray dose of 1,200 r was given and that all the increase is in a lineal fashion. The regression lines of the relative values, (i. e., taking each control value as 1) of all the values obtained, and the regression lines of cell volume, protein and NP-SH are identical, whereas those of NP-SS and P-SH appear to be widely seperated. However, the difference of those two lines (NP-SS & P-SH) were found to be not significant statistically (p>0.05). Therefore, it can be concluded from the above results that all the values examined increase in a lineal fashion with no statistically significant difference among them. Also, with the radiation dose of 1,200 r, the ELD cell becomes enlarged and swollen progressively up to 60 hours post-irradiation and it becomes more than two times of the original normal size at 60 hours after the irradiation, and up to this stage, it seems apparent that the cell division has been slow due to the X-irradiation applied in this experiment. It is well understandable that the contents of NP-SH, NP-SS, P-SH and protein of the ELD cells increase in parallel with the increase of the cell volume by the X-ray does used, but it also seems interesting to note that all the cellular substances tested show no appreciable difference in the pattern of increase.

• The Korean Journal of Physiology
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• v.18 no.2
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• pp.163-169
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• 1984

The effect of vanadate on the optimum pH of Na-K-ATPase was investigated. The results were as follows: 1) The optimum PH of Na-K-ATPase was shifted from PH 7.4 to 6.8 at 10 mM K by $5{\times}10^{-6}M$ vanadate. 2) The ratio of Na-K-ATPase activity at pH 6.8 and 7.4 increased with increasing vanadate concentration. 3) Inspite of the presence of $5{\times}10^{-6}M$ vanadate Na-K-ATPase activity at pH 7.4 was higher than that at pH 6.8 below 50 mM $Na^+$, and the ratio of Na-K-ATPase activity at pH 7,4 and 6.8 was higher than that of the control. 4) Na-K-ATPase activity at pH 7.4 was higher than that at pH 6.8 below 7mM $K^+$. 5) Optimum pH of Na-K-ATPase activity was shifted from pH 7.4 to 6.8 by $10^{-5}M$ vanadate at 5 mM $K^+$. 6) $K^+$-pNPPase activity increased with lowering of pH, and the degree of inhibition of $K^+$-pNPPase activity by $10^{-7}$M vanadate was decreased with lowering of pH. These results suggest that vanadate shifts the optimum pH of Na-K-ATPase activity to more acidic PH than PH 7.4. This effect may not be caused by the decrease in the inhibitory potency of vanadate itself to Na-K-ATPase by the change of medium pH, but mainly by the alteration of Na-and K-binding site, which appears in the presence of vanadate only.

• The Korean Journal of Physiology
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• v.18 no.2
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• pp.125-137
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• 1984

The effects of external $Ca^{2+}$ ana the inhibition of Na-pump on vanadate-induced contraction in isolated human and rat uterine smooth muscle were studied and the following results were observed. 1) Vanadate induced contraction in rat uterine muscle and showed maximal contraction at concentration of $5{\times}10^{-4}$M, and the contractile response to vanadate was more sensitive in human than rat uterine muscle. 2) Vanadate-induced contraction was not completely inhibited by $Ca^{2+}$ removal from PSS and the response to $Ca^{2+}$ removal was more sensitive in human than rat uterine muscle. 3) Vanadate-induced contraction decreased with increasing concentration of verapamil, but even in the presence of $3{\times}10^{-5}M$ verapamil which inhibited 100 K-induced contraction completely. about 40% of maximal contraction remained, and its amplitude was similar to that of contraction in $Ca^{2+}$-free solution. 4) Vanadate-induced contraction was increased by the inhibition of Na-pump and this increase also could be observed in the presence of $3{\times}10^{-5}M$ verapamil. 5) After pretreatment with $Ca^{2+}$-free PSS containing ouabain Vanadate-induced contraction was not increased, but the contractile response of these tissues to the addition of external $Ca^{2+}$ was remarkably increased in the presence of vanadate. 6) $3{\times}10^{-5}$M verapamil inhibited vanadate-induced $Ca^{45}$ influx completely, but after pretreatment with ouabain vanadate could induce remarkable $Ca^{45}$ influx even in the presence of verapmil. 7) With increasing the time of pretreatment with ouabain or $K^+$-free solution, the degree of increase in contraction by vanadate was more remarkable. 8) $10^{-4}M$ papaverine stowed a considerable inhibition of the increase in the vanadate-induced contraction by pretreatment with ouabain. 9) Acetylcholine-induced contraction increased with lengthening the duration of Na-pump inhibition even in the presence of verapamil. Considering above results it seems that the uterine muscle of human is more sensitive to vanadate than that of rat, and both internal and external $Ca^{2+}$ is utilized in vanadate·induced contraction. In the case of Na-pump inhibition several smooth muscle contracting agents seems to induce $Ca^{2+}$ influx which is not inhibited by verapamil. This $Ca^{2+}$ influx seems to be inhibited by papaverine and to be associated with membrane potential, although its precise characteristics is not certain.

• The Korean Journal of Physiology
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• v.4 no.1
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• pp.29-46
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• 1970

Measurement and analysis of the physical status (height, body weight, breast girth, sitting height. length of leg, length of thigh, thigh girth, crural length, length of arm, brachial length, antebrachial girth and skinfold thickness), physical types and the growth were made to the 360 Korean middle and high school boys aged between 12 and 17 years in Taegu City. The physical status was evaluated and expressed as dispersion and the Physical type as percentage of each status to height, and the growth was analysed by the growth formula. The results are as follows; 1) The increase of the volumes of Physical status was slowest between 12 and 13 years and fastest between 13 and 14 years in general. 2) The increase of the volumes of thigh girth and antebrachial girth showed a linear pattern until 16 years. 3) The coefficient of variation was largest in skinfold thickness $(16.3{\sim}28.4%)$ followed by body weight $(10.0{\sim}14.3%)$, antebrachial girth $(4.8{\sim}19.60%)$ and length of thigh$(6.3{\sim}13.6%)$. The coefficients of variation in all the other status were similar $(4{\sim}7%)$. 4)The physical indices of body weight, breast girth, sitting height, length of thigh, thigh girth, antebrachial girth and skinfold thickness increased as age increased while the others decreased except the brachial length, which showed no significant change. 5) Ratio of growth quantity was largest in body weight followed by skinfold thickness, and the others were all similar. 6) Growth rate and specific growth rate decreased in the all the status analysed as age increased except in the skinfold thickness in which an increase was noted. 7) Growth gradient was increased along the increase of age in breast girth, sitting height, crural length, brachial length and in skinfold thickness. However a decrease was observed in the other status except in the body weight which was decreased until 15 years of age and increased thereafter.

• The Korean Journal of Physiology
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• v.16 no.2
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• pp.195-207
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• 1982

To determine the influence of vegetarian diet on serum lipoprotein, cholesterol and protein levels, 45 young Buddhist nuns (age: $20{\sim}34$ years) and 29 female students(age: $20{\sim}22$ years) were examined. Daily caloric intakes were 1,945 Kcal for the Buddhist nuns and 1,815 Kcal for the students. The ratio of% calorie of carbohydrate: protein: fat from total calories in the Buddhist nuns was 84:11:5 and that in the students was 70:15:15. The Buddhist nuns had significantly higher carbohydrate intake but markedly lower lipid intakes than the students. Anthropometric measurement showed that the Buddhist nuns had significantly higher values of body weight, skin-fold thickness, body surface area and obesity index than the students. Both systolic and diastolic pressures of the Buddhist nuns and students were similar. Serum levels of total lipid, cholesterol and proteins in the Buddhist nuns were not different from those of the students. However, when comparing the levels of high density lipoprotein (HDL), very low density lipoprotein (VLDL) and low density lipoprotein (LDL) fractions, the Buddhist nuns had lower level of HDL but significantly higher LDL levels than the students. Furthermore, the Buddhist nuns had significantly lower levels of serum HDL-cholesterol but significantly higher LDL-cholesterol levels. There were significant correlations between LDL and LDL cholesterol (r=0.40), VLDL and VLDL-cholesterol(r=0.85), HDL and HDL-cholesterol(r=0.45), total serum lipid and total cholesterol (r=0.66) and total serum cholesterol and LDL(r=0.79). On the other hand, values of both serum total protein, and fractions of serum proteins were similar in the Buddhist nuns and students(ratio of albumin: ${\alpha}_{1}-:\;{\alpha}_{2}-:\;{\beta}-:\;{\gamma}-$globulins=55:3:10:13:19). Hematocrit and hemogloblin levels were similar in the Buddhist nuns and students. Above results suggest that vegetarian diets of the Buddhist nuns produced alterations in the metabolism of the lipoproteins and cholesterol.

• The Korean Journal of Physiology
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• v.16 no.2
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• pp.187-193
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• 1982

The relationship between water and electrolyte metabolism, and dietary intake were studied in 45 healthy Buddhist nuns who were vegetarians aged 20-34, and 28 nursing students aged 20-22 who stayed at the dormitory of Kyungpook Medical School in the Fall, 1981. The Buddhist nuns obtained significantly higher carbohydrate and total caloric intakes but significantly lower protein and lipid intakes than the female students. The Buddhist nuns excreted significantly higher urine output($1,697{\pm}68\;ml/day$, p<0. 05) and lower osmolality ($616{\pm}18\;mOsm/kg\;H_2O$, p<0.05) than the students ($1,505{\pm}67\;ml/day$ and $688{\pm}36\;mOsm/kg\;H_2O$). However, both groups excreted similar amounts of urinary $Na^+$, $K^+$ and total osmolar contents. Free water clearance of the Buddhist nuns was higher(p<0.05) than that of the students but the osmolar clearance was about the same in the two groups. Daily urine output showed good correlation with Na output (r=0.76) and osmolar clearance but not with free water clearance. Both groups showed similar values of plasma concentration of creatinine, daily excretion of creatinine and clearance. Urinary excretion of urea for Buddhist nuns was 6.4 g/day, and was significantly higher than that of the students (5.1g/day).

• The Korean Journal of Physiology
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• v.13 no.1_2
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• pp.1-12
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• 1979

To evaluate the present status of physical fittness of Korean long distance runners, body fat, pulmonary functions, maximal oxygen intake and oxygen debt were measured in 5 elite marathoners (A group), 6 college student runners (B group) and 3 middle school student runners (C group). After laboratory tests, full course marathon running was performed in 2 elite marathoners during which their heart rates were monitored continuously. The results are summerized as follows: 1) Total body fat in all three groups are in the range of 13-15% of their body weight. 2) In all three groups, average values of various pulmonary functions were within the normal limits, but those of tidal volume were higher and respiratory rate were lower in comparison to normal values. These phenomena may represent respiratory adaptations against training. The average resting oxygen consumptions in A,B and C were $322{\pm}23$, $278{\pm}14$ and $287{\pm}16$m1/min, respectively. 3) In all three groups, resting blood pressures were in the normal range, but the resting heart rate was slightly lower in groups A $(56{\pm}3\;beats/min)$ and B $(64{\pm}2\;beats/min)$ and higher in group C $(82{\pm}9\;beats/min)$ in comparison to normal values. These changes in cardiovascular functions in marathoners may also represent adaptive phenomena. 4) During treadmill running the minute ventilation and oxygen consumption of the runners increased lineally with work load in all three groups. When the oxygen consumption was related to heart rate, it appeared to be a exponential function of the heart rate in all three groups. 5) The average maximal heart rates during maximal work were $196{\pm}3$, $191{\pm}3$ and $196{\pm}5\;beats/min$ for groups A,B and C, respectively. Maximal oxygen intakes were $84.2{\pm}3.3\;ml/min/kg$ in group A, $65.2{\pm}1.1\;ml/min/kg$ in group B and $58.7{\pm}0.4\;ml/min/kg$ in group C. 6) In all three groups, oxygen debts and the rates of recovery of heart rate after treadmill running were lower than those of long ditsance runners reported previously. 7) The 40 km running time in 2 elite marathoners was recorded to be $2^{\circ}42'25'$, and their mean speed was 243 m/min (ranged 218 to 274 m/min). The heart rate appeared to increase lineally with running speed, and the total energy expenditure during 40 km running was approximately 1360.2 Calories. From these it can be speculated that if their heart rates were maintained at 166 beats/min during the full course of marathon running, their records would be arround $2^{\circ}15'$. Based on these results, we may suspect that a successful long distance running is, in part, dependent on the economical utilization of one's aerobic capacity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.3
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• pp.293-298
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• 2014

This study conducted experiments on the reclaimed land of Saemangeum located in Jeongrabuk-do in order to gain basic information about growth characteristics and yield ability according to soil salinity. Having soil excluding salt as a control group, this study adjusted the specimens' soil salinity to level 4 and then planted four varieties including Ilmichal Corn to investigate the growth or grain yield according to salinity. About the corn establishment rate according to soil salinity, over 97% up to $3.2dS\;m^{-1}$ was established, and then, it was reduced gradually according to the increase of concentration. According to the salt concentration of soil more required growth duration from seeding to heading comparing to non-treatment salt was delayed, at $1.6dS\;m^{-1}$, two days were delayed, at $3.2dS\;m^{-1}$, four to six days were delayed differently by varieties, and at $4.8dS\;m^{-1}$, six to 10 days were delayed. About the plant fresh weight according to soil salinity, Chalok 4 and Eolrukchal indicated 93%~97% or so compared with the salt-free one at $1.6dS\;m^{-1}$, and Chalok No. 4 showed 79% at the salinity of $3.2dS\;m^{-1}$, too, and it was a relatively higher growth percentage than those of the other varieties. In terms of dried seed weight according to soil salinity, compared with the corns cultivated in the control group, averagely 12.1% was lowered at the time of cultivation at $1.6dS\;m^{-1}$, and $3.2dS\;m^{-1}$ 40% was lowered, and about 70% was lowered at $4.8dS\;m^{-1}$. According to the result of examining the point of time that dried seed start to reduce due to soil salinity with the regression equation, soil salinity which starts the reduction of grain weight is $1.67dS\;m^{-1}{\sim}2.18dS\;m^{-1}$, and it differs by varieties, and EC of 50% that the yield reduces in half is $2.96dS\;m^{-1}{\sim}4.45dS\;m^{-1}$. And the degree of influence on each of the growth factors according to soil salinity is founded to be in the order of establishment rate