• Animal cells and systems
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• 제6권1호
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• pp.13-18
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• 2002

Phylogenetic relationships of Korean Acidiella species were tested using mitochondrial 16S ribosomal RNA gene sequences. We used 16 published sequences as outgroup, and 10 new sequences for nine Korean Acidiella species as ingroup. The number of aligned sites was 1,281 bp, but 1,135 bp were used for the analysis after excluding sites with missing data or gaps. Among these 1,135 sites, 464 sites were variable and 340 were informative for parsimony analysis. Phylogenetic information was extracted from this data set using neighbor-joining, maximum likelihood and maximum parsimony methods and compared to a morphology-based phylogenetic hypothesis. Our molecular data suggest that: (1) the tribe Trypetini appears to be monophyletic even when the nine additional Acidiella species are added to our previous phylogenetic analysis; (2) all the Korean Acidiella species belong to the Trypeta group, but the genus Acidiella is not supported as monophyletic; (3) the close relationship of A. circumvaga, A. issikii, and A. sapporensis is supported; (4) the close relationship of A. pachypogon and two additional new Acidiella species is strongly supported; and (5) the possible presence of two or more cryptic species among the specimens previously identified as A. obscuripennis is suggested. Sequence data from the mitochondrial 16S rDNA allowed us to better understand the systematic status of Korean Acidiella species. They indicated that the current concept about the genus Acidiella is insufficient and needs to be refined further. This study also showed a few interesting relationships, that had not been recognized by morphological study alone. Based on this study, we were able to plan further experiments to analyze relationships within the Trypeta Group.

• Parasites, Hosts and Diseases
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• 제53권2호
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• pp.201-207
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• 2015

This study aimed to investigate the infection status, worm development, and phylogenetic characteristics of the intestinal trematode, Stellantchasmus falcatus. The metacercariae of S. falcatus were detected only in the half-beak (Dermogenus pusillus) out of the 4 fish species examined. Their prevalence was 90.0%, and the intensity of infection was 919 metacercariae on average. Worms were recovered from 33 (97.1%) of 34 chicks that were experimentally infected with 200 S. falcatus metacercariae each, and the average recovery rate was 43.0%. The body size and inner organs of S. falcatus quickly increased in the experimental chicks over days 1-2 post-infection (PI). In addition, ITS2 sequence data of this parasite were analyzed to examine the phylogenetic relationships with other trematodes using the UPGMA method. The results indicated that the ITS2 sequence data recorded from trematodes in the family Heterophyidae appeared to be monophyletic. This study concluded that D. pusillus serves as a compatible second intermediate host of S. falcatus in Thailand and that S. falcatus can develop rapidly in the experimental chicks. Data collected from this study can help to close the gap in knowledge regarding the epidemiology, biology, and phylogenetic characteristics of S. falcatus in Thailand.

• Animal Systematics, Evolution and Diversity
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• 제28권1호
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• pp.36-41
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• 2012

The larvae of Chaoborus are widely distributed in lakes, ponds, and reservoirs. These omnivorous Chaoborus larvae are crucial predators and play a role in structuring zooplankton communities, especially for small-sized prey. Larvae of Chaoborus are commonly known to produce predator-induced polyphenism in Daphnia sp. Nevertheless, their taxonomy and molecular phylogeny are very poorly understood. As a fundamental study for understanding the role of Chaoborus in predator-prey interactions in a freshwater ecosystem, the molecular identification and phylogenetic relationship of Chaoborus were analyzed in this study. A molecular comparison based on partial mitochondrial cytochrome oxidase I (COI) between species in Chaoborus was carried out for the identification of Chaoborus larvae collected from 2 localities in Korea. According to the results, the Chaoborus species examined here was identified as C. flavicans, which is a lake-dwelling species. Furthermore, partial mitochondrial genome including COI, COII, ATP6, ATP8, COIII, and ND3 were also newly sequenced from the species and concatenated 5 gene sequences excluding ATP8 with another 9 dipteran species were compared to examine phylogenetic relationships of C. flavicans. The results suggested that Chaoborus was more related to the Ceratopogonidae than to the Culicidae. Further analysis based on complete mitochondrial DNA sequences and nuclear gene sequences will provide a more robust validation of the phylogenetic relationships of Chaoborus within dipteran lineages.

• Mycobiology
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• 제46권3호
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• pp.192-204
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• 2018

The name Golovinomyces cynoglossi s. lat. is traditionally applied to a complex of morphologically similar powdery mildews on hosts of the plant family Boraginaceae. The current species-level taxonomy within this complex is ambiguous due to the lack of phylogenetic examinations. The present study applied phylogenetic methods to clarify the taxonomy of G. cynoglossi s. lat. Phylogenetic analysis of rDNA ITS sequences retrieved from Asian, European and North American specimens revealed that G. cynoglossi s. lat. collections from different hosts involved several species in five clearly separated lineages. Clade I consists primarily of Golovinomyces cynoglossi s. str. on Cynoglossum. Clade III consists of Golovinomyces sequences retrieved from the host genera Symphytum and Pulmonaria. The taxa within clade III are now assigned to G. asperifoliorum comb. nov. Clade V encompasses G. cynoglossi s. lat. on the host genera Bothriospermum, Buglossoides, Echium, Myosotis, and Trigonotis. The taxa within clade V are now assigned to G. asperifolii comb. nov. The species concerned in this study were lecto- and epitypified to stabilize their nomenclature.

• Journal of Microbiology
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• 제35권2호
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• pp.79-86
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• 1997

For the phylogenetic study of the genus Trichaptum, nuclear ribosomal DNA sequences from eight strains of four Trichaptium species were examined. Phylogenetic trees were constructed using molecular data on 18 rDNA and 5.8S rDNA and thei ITSs. Parsimony analyses of the Trichaptum species showed that T. biforme and T. laricinum made a monophyletic group respectively, suggesting that each species is phylogenetically independent. However, T. abietum represented a polyphyletic group and T. fusco-violaceum formed a polytomous group, suggesting that these species could be in the process of evolutionary differentiation. Examination of base substitutions of the 18S rRNA gene reveals that the C-T transition is most predominant and that there is a stronger transition bias between closely related organisms rather than between distantly related ones.

• ALGAE
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• 제17권2호
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• pp.75-81
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• 2002

In this study the length of ITS2 from four species of the Ulvaceae in Jeju Island varied between 167 and 203 bp. The resuits of this investigation showed that two genus, Ulva and Enteromorpha are grouped in a monophyletic assemblage with 100% bootstrap support in all phylogenetic trees. However, a thorough eamination of these characters from representatives does not provided a way to identify any unique morphological features of clasdes in this tree. This study reveals that Ulva conglobata and Ulva pertusa belong to one clade in the phylogenetic tree with the samples from Jeju Island, Korea.

• Mycobiology
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• 제35권3호
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• pp.117-123
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• 2007

Seventy-two lichen specimens of Cetrelia collected in South Korea since 2003 were examined by both phenotypic and phylogenetic analyses. The phenotypic analysis was based on morphological and chemical characters, and the phylogenetic analysis was based on nrDNA ITS sequences. The result suggested that the presence and absence of isidia, soredia, lobules and medullar reaction C+ or C- are the important characters in the taxonomy of this genus. Four species of Cetrelia, C. chicitae, C. braunsiana, C. japonica, and C. pseudolivetorum have been identified in this study. Description of each species is presented with morphological and chemical characters. A key to the Cetrelia species is also presented.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.70-70
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• 2022

Corchoropsis Siebold & Zucc. was traditionally included in Tiliaceae, however, several morphological characters and molecular phylogenetic studies supported that it was the member of Dombeyoideae (Malvaceae). It is used as compost, medicinal, and ornamental herbs. Out of total three species, two species and one variety, grow in Korea. Although it is an East Asian endemic genus, plastome data has not been defined. Here, we characterize the plastome sequences of C. tomentosa (160,093 bp) and C. tomentosa var. psilocarpa (160,724 bp). Corchoropsis encodes 78 protein-coding genes, 30 tRNAs, and four rRNAs with a pseudogene inf A. Phylogenetic study revealed that Tilioideae is sister of Dombeyoideae. This study will contribute to define the genome structures, phylogenetic, and evolutionary studies of the genus Corchoropsis.

• Mycobiology
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• 제51권4호
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• pp.210-215
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• 2023

The aim of this study was to characterize a new fungal species, Acremonium conglutinatum, isolated from air samples collected in Wando, South Korea. Phylogenetic analysis based on the internal transcribed spacer and large subunit regions revealed its unique position within the genus Acremonium. The isolated strain displayed distinct morphological characteristics, including ellipsoid or bent-ellipsoid conidia formed in clusters on the phialides. These features differentiate the new species from closely related species within the genus. This study describes the morphological and molecular characteristics of A. conglutinatum and emphasizes its phylogenetic relationships with other Acremonium spp. The identification of this novel species contributes to our understanding of the diversity and ecological role of Acremonium.

• 미생물학회지
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• 제38권4호
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• pp.293-298
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• 2002

다양한 농도의 영양배지(nutrient broth, NB; diluted nutrient broth, DNB)를 이용하여 음용 지하수중의 세균수를 계수한 결과, 통상농도의 NB배지에 비해 저영양배지인 DNB배지에서 2～50배 이상 높은 계수치를 나타내었다. 이와 같은 결과로부터 지하수중에는 NB배지에서는 중식이 현저히 저해되고 DNB배지에서만 중식이 가능한 저영양세균(oligotrophic bacteria)이 다수 분포하고 있음이라 판단되어 DNB배지로부터 184균주의 저영양세균을 분리하였다. 본 연구에서는 시판 음용수(CW, CJ )와 광천수(DPG, CJG1)에서 분리된 저영양세균 102 균주의 165 rDNA 염기서열을 결정하여 계통분류학적 특성을 검토한 결과 세 개의 주요한 계통군: Proteobacteria $\alpha$-subdivision (49 균주), $\beta$-subdivision (50 균주), $\gamma$-subdivision (3 균주)으로 분류되었다. Proteobacteria $\alpha$-subdivision에는 Afipia, Blastobacter, Bradyrhizobium, Caulobacter, Phenylobacterium, Rhizobioum, Sphingomonas가 포함되었으며, $\beta$-subdivision에는 Acidovorax, Azonexus, Ferribacterium, Janthinobacterium, Leptothrix, Polaromonas, Variovorax가 포함되었고, $\gamma$-subdivision에는 Rhodanobacter 등 다양한 계통군이 확인되었다.