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Phylogenetic Analysis of Oligotrophic Bacteria Found in Potable Groundwater  

Tomoyoshi Hashimoto (Department of Agro-Environmental Research, National Agricultural Research Center)
Publication Information
Korean Journal of Microbiology / v.38, no.4, 2002 , pp. 293-298 More about this Journal
Abstract
In order to investigate the ecological aspect of bacteria on groundwater, water samples were collected from various regions. Total of 318 strains were isolated from diluted nutrient broth (DNB) agar medium, and investigated their growth pattern on nutrient broth (NB) medium. As a result, all the isolated strains were divided into two groups, NB and DNB organisms. Growth of DNB organisms were suppressed in full strength NB medium but not in DNB medium, which were called oligotrophic bacteria in this study. Proportion of DNB organisms occurred in the frequency of 50-98% in potable groundwaters (CW, CJ, DPG, CJG1), however, it was 23,46% in polluted site (TJ, NPG1). One hundred and two strains were identified as oligotrophic bacteria and their phylogenetic characteristics were determined by using 16S rDNA sequencing. Based on the phylogenetic analysis, they were found to fall into three major phylogenetic groups: belonging to the Proteobacteria $\alpha$-(49 strains), $\beta$-(50 strains), $\gamma$ -(3 strains) subdivisions. The phylogenetic analysis suggested that microbial diversity of potable groundwater is more complex than that obtained in the past investigation.
Keywords
oligotrophic bacteria; phylogenetic analysis; potable groundwater; proteobacteria $\alpha$-; $\beta$-; $\gamma$-subdivision.;
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1 /
[] / 먹는물 수질기준 및 검사등에 관한 규칙(환경부령 제 65호, 1999.2.11) 제2조 별표 1.
2 Kogure, K. 1997. 病原細菌 Viable but Nonculturable (VBNC) State. Microbes and Environments. 12, 135-145
3 The neighbor-joining method: a new method for reconstructing phylogenetic trees /
[ Saitou, N.;M. Nei ] / Mol. Biol. Evol.
4 Viable but non-culturable Vibrio cholerae and related pathogens in the environment: implications for relase of genetically engineered microorganisms /
[ Colwell, R.R.;P.R. Brayton;D.J. Grimes;D.B. Roszak;S.A. Huq;L.M. Palmer ] / Bio. Technol.
5 Vibrios in the environment:viable but nonculturable vibrio cholerae /
[ Colwell, R.R.;S.A. Huq:I.K. Wachsmuth(ed.);P.A. Blake(ed.);O. Osvik(ed.) ] / Vibrio cholerae and cholerae: molecular to global perspectives
6 Biology of oligotrophic bacteria /
[ Kuznetsov, S.I.;G.A. Dubinnina;N.A. Lapteva ] / Ann. Rev. Microbiol.   ScienceOn
7 CLUSTALW; improving the sensitivity of progressive multiple sequence alignment through sequence weighting positions specific gap penalties and weight matrix choice /
[ Thomson, J.D.;D.G. Higgins;T.J. Gibson ] / Nucleic Acids Res   ScienceOn
8 Oligotrophic bacteria on organic debris and plant roots in paddy field soil /
[ Ohta, H.;T. Hattori ] / Soil Biol. Biochem.   ScienceOn
9 Microbiological safety of natural mineral water /
[ Leclerc, H.A. Moreau ] / FEMS Microbiol. Rev.   ScienceOn
10 Plate count of bacteria in soil on diluted nutrient broth as a culture medium /
[ Hattori, T. ] / Rep. Inst. Agric. Res.
11 형광현미경을 이용한 음용 지하수내 배양불능 세균의 관찰 및 정량적 평가 /
[ 김인기;染容孝;황경숙 ] / 한국미생물학회지   과학기술학회마을
12 Similarity analysis of rRNAs /
[ Johnson, J.L.;gerhardt, P.(ed.);R.G.E. Murray(ed.);W.A. Wood(ed.);N.R. Kring(ed.) ] / Methods for general and molecular bacteriology
13 A new method for enumeration of oligotrophic bacteria in lake water /
[ Ishida, Y.;H.Kadota ] / Arch. Hydrobiol.
14 Microbiological safety of drinking water /
[ Szewzyk, U.;R. Szewzyk;W. Manz;K.H. Schleifer ] / Annu. Rev. Microbiol.   ScienceOn
15 Advance in soil microbial ecology and biodiversity /
[ Hattori, T.;Samson, R.A.(ed.);Stalper, J.A.(ed.);Mei, D.(ed.);Stouthamer, A.H.(ed.) ] / Proceedings of the eighth international congress for culture collections
16 Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride /
[ Heng Zhu;Feng Qu;Li-Huang Zhu ] / Nucleic Acids Res.   ScienceOn
17 Oligotrophic bacteria in rendzina a forest soil /
[ Whang, K.;T. Hattori ] / Antonie van Leewenhek.   ScienceOn
18 病原細菌 Viable but Nonculturable (VBNC) State /
[ Kogure, K. ] / Microbes and Environments.
19 Starvation and miniaturization of heterotrophs, with special emphasis on maintenance of the starved viable state /
[ Morita, R.Y.;Fletcher M.(ed.);Floodagate GD(ed.) ] / Bacteria in their natural environment
20 16S/23S rRNA sequencing /
[ Lane, D.J.;Stackebrandt, E.(Ed.);M Goodfellow(Ed.) ] / Nucleic acid Techniques in bacterial systematics
21 Oligotrophy. Fast and famine existence /
[ Poindexter, J.S.:M. Alexander(ed.) ] / Advances in microbiol ecology