Animal Systematics, Evolution and Diversity

The Korean Society of Systematic Zoology (한국동물분류학회)
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Molecular Taxonomy of a Phantom Midge Species (Chaoborus flavicans) in Korea

  • An, Hae-In (Department of Green Life Science, Sangmyung University) ;
  • Jung, Gil-A (Department of Green Life Science, Sangmyung University) ;
  • Kim, Chang-Bae (Department of Green Life Science, Sangmyung University)
  • Received : 2011.10.10
  • Accepted : 2011.12.26
  • Published : 2012.01.31
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Abstract

The larvae of Chaoborus are widely distributed in lakes, ponds, and reservoirs. These omnivorous Chaoborus larvae are crucial predators and play a role in structuring zooplankton communities, especially for small-sized prey. Larvae of Chaoborus are commonly known to produce predator-induced polyphenism in Daphnia sp. Nevertheless, their taxonomy and molecular phylogeny are very poorly understood. As a fundamental study for understanding the role of Chaoborus in predator-prey interactions in a freshwater ecosystem, the molecular identification and phylogenetic relationship of Chaoborus were analyzed in this study. A molecular comparison based on partial mitochondrial cytochrome oxidase I (COI) between species in Chaoborus was carried out for the identification of Chaoborus larvae collected from 2 localities in Korea. According to the results, the Chaoborus species examined here was identified as C. flavicans, which is a lake-dwelling species. Furthermore, partial mitochondrial genome including COI, COII, ATP6, ATP8, COIII, and ND3 were also newly sequenced from the species and concatenated 5 gene sequences excluding ATP8 with another 9 dipteran species were compared to examine phylogenetic relationships of C. flavicans. The results suggested that Chaoborus was more related to the Ceratopogonidae than to the Culicidae. Further analysis based on complete mitochondrial DNA sequences and nuclear gene sequences will provide a more robust validation of the phylogenetic relationships of Chaoborus within dipteran lineages.

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References

  1. Cameron SL, Lambkin CL, Barker SC, Whiting MF, 2007. A mitochondrial genome phylogeny of Diptera: whole genome sequence data accurately resolve relationships over broad timescales with high precision. Systematic Entomology, 32: 40-59. https://doi.org/10.1111/j.1365-3113.2006.00355.x
  2. Dupuis DD, Svensson JE, Taylor DJ, 2008. The cryptic origins of environment-indicating phantom midges (Chaoborus). Limnology and Oceanography, 53:236-243. https://doi.org/10.4319/lo.2008.53.1.0236
  3. Friedrich M, Tautz D, 1997. Evolution and phylogeny of the Diptera: a molecular phylogenetic analysis using 28S rDNA sequences. Systematic Biology, 46:674-698. https://doi.org/10.1093/sysbio/46.4.674
  4. Jeong GR, 2010. Seasonal and diel abundance and feeding patterns of Chaoborus flavicans in Sang-Chun reservoir. MS thesis, Ajou University, Suwon, Korea, pp. 1-44.
  5. Kambhampati S, Smith PT, 1995. PCR primers for the amplification of four insect mitochondrial gene fragments. Insect Molecular Biology, 4:233-236. https://doi.org/10.1111/j.1365-2583.1995.tb00028.x
  6. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG, 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23:2947-2948. https://doi.org/10.1093/bioinformatics/btm404
  7. Lee ES, Shin KS, Kim MS, Park H, Cho S, Kim CB, 2006. The mitochondrial genome of the smaller tea tortrix Adoxophyes honmai (Lepidoptera: Tortricidae). Gene, 373:52-57. https://doi.org/10.1016/j.gene.2006.01.003
  8. Miller BR, Crabtree MB, Savage HM, 1997. Phylogenetic relationships of the Culicomorpha inferred from 18S and 5.8S ribosomal DNA sequences (Diptera: Nematocera). Insect Molecular Biology, 6:105-114. https://doi.org/10.1111/j.1365-2583.1997.tb00078.x
  9. Nylander JAA, 2004. MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Uppsala.
  10. Ronquist F, Huelsenbeck JP, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19: 1572-1574. https://doi.org/10.1093/bioinformatics/btg180
  11. Saether OA, 2000. Phylogeny of Culicomorpha (Diptera). Systematic Entomology, 25:223-234. https://doi.org/10.1046/j.1365-3113.2000.00101.x
  12. Simon JC, Pfrender ME, Tollrian R, Tagu D, Colbourne JK, 2011. Genomics of environmentally induced phenotypes in 2 extremely plastic arthropods. Journal of Heredity, 102: 512-525. https://doi.org/10.1093/jhered/esr020
  13. Swofford, DL, 2003. $PAUP^{\ast}$: Phylogenetic Analysis Using Parsimony ($^{\ast}$and other methods). Sinauer Associates, Sunderland, MA.
  14. The Entomological Society of Korea and Korean Society of Applied Entomology, 1994. Checklist of insects from Korea. Konkuk University Press, Seoul, pp. 1-744.
  15. Tollrian R, Dodson SI, 1999. Inducible defenses in Cladocera: constraints, costs, and multipredator environments. In: The ecology and evolution of inducible defenses (Eds., Tollrian R, Harvell CD). Princeton University Press, Princeton, NJ, pp. 177-202.

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