• 제목/요약/키워드: phylogenetic relationship

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팽이버섯(Flammulina velutipes)의 Genome-wide SNP (Single Nucleotide Polymorphism)에 의한 계통 분석 (Genome-wide Single Nucleotide Polymorphism-based Assay for Phylogenetic Relationship of the Flammulina velutipes)

  • 우성이;김은선;한재구;장갑열;신평균;오연이;오민지;조성환;이정희;김경수;공원식
    • 한국균학회지
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    • 제43권4호
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    • pp.231-238
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    • 2015
  • 팽이버섯(Flammulina velutipes) 25품종의 유전체 재분석 데이터를 표준유전체(KACC42781)와 비교하여 genomewide single nucleotide polymorphism (SNP)를 선발하였다. 균주에 따른 mapping율의 차이는 균주간 변이를 반영하였으며, genome-wide SNP분포는 homozygous SNP, heterozygous SNP로 구분되었으며 모두 균주에 따른 변이가 크게 나타났다. 수집균주들 사이의 유연관계를 살펴보기 위해, 계통수를 그려본 결과, Group I은 F. velutipes var. 계통인 ASI 4062, 4148, 4195이 묶여지고, Group II는 ASI 4188 F. elastica, ASI 4190 F. fennae, ASI 4194 F. rossica의 다른 종이 별도의 그룹을 형성하였다. 그 외 F. velutipes 19개 계통은 같은 그룹으로 나타났으며 그 유전적 자리를 잘 반영하였다. 한편 백색 group과 갈색 group을 유연관계로 분석하고자 시도하였으나 색깔에 따른 group은 이루어지지 않았다. 한국 백색 품종인 ASI 4210, 4166, 4178과 일본 백색 품종인 ASI 4209, 4167을 분석한 결과 phylogenetic tree상에서 한국 백색 품종과 일본 백색 품종간의 유전적 상동성이 매우 높음을 확인할 수 있었다.

Phylogenetic analyses reveals two unrecognized species of Sparganium (Typhaceae) in the Korean Peninsula

  • Gil, Hee-Young;Ha, Young-Ho;Choi, Kyoung Su;Chang, Kae Sun;Choi, Kyung
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 추계학술대회
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    • pp.42-42
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    • 2018
  • Two unrecognized species of Sparganium of Korea were found during the our field expeditions and phylogenetic analyses of specimens deposited in the Herbarium of Korea National Arboretum (KH). S. coreanum H. $L{\acute{e}}v$. was first reported as a new species based on the specimen (Taquet, 2150) collected from Jeju Island. It has been recognized as synonym or infraspecific taxa of S. stoloniferum and S. eurycarpum or even never recognized recently. However, phylogenetic tree showed that S. coreanum is monophyletic and has sister relationship with S. eurycarpum. Furthermore, additional distribution localities were also found by herbarium survey. Morphological characteristics and distribution information of S. coreanum will be discussed. Another Sparganium species found from Mt. Daeam is occurring either as floating or emergent. Although we could not identify this species since lack of any flowers or fruits for two year surveys, phylogenetic analyses results showed that this species belong to the clade of S. glomeratum, which is distributed in high elevation lakes and marshes of Europe, Asia, and North America. Additional survey of morphology and report will be needed.

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Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences

  • Son, OGyeong;Yoon, Chang Young;Park, SeonJoo
    • 한국자원식물학회지
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    • 제27권6호
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    • pp.671-679
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    • 2014
  • Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.

Morphological Identification and Phylogenetic Analysis of Laelapin Mite Species (Acari: Mesostigmata: Laelapidae) from China

  • Yang, Huijuan;Yang, Zhihua;Dong, Wenge
    • Parasites, Hosts and Diseases
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    • 제60권4호
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    • pp.273-279
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    • 2022
  • Laelapinae mites are involved in transmission of microbial diseases between wildlife and humans, with an impact on public health. In this study, 5 mite members in the subfamily Laelapinae (laelapin mites; LM) were morphologically identified by light microscopy, and the phylogenetic relationship of LM was analyzed in combination with the sequence information of part of the LM cytochrome oxidase subunit I (cox1) gene. The morphological identification revealed that 5 mites belonged to the genera Laelaps and Haemolaelaps, respectively. Sequence analysis showed that the ratio of nonsynonymous mutation rate to synonymous mutation rate of LM was less than 1, indicating that the LM cox1 gene had undergone purifying selection. Phylogenetic analysis showed that the Laelapinae is a monophyletic group. The genera Haemolaelaps and Hyperlaelaps did not separated into distinct clades but clustered together with species of the genus Laelaps. Our morphological and molecular analyses to describe the phylogenetic relationships among different genera and species of Laelapinae provide a reference for the improvement and revision of the LM taxonomy system.

Study on Phylogenetic Relationship Between Wild Japanese Quails in the Weishan Lake Area and Domestic Quails

  • Chang, G.B.;Chang, H.;Zhen, H.L.;Liu, X.P.;Sun, W.;Geng, R.Q.;Yu, Y.M.;Wang, S.C.;Geng, S.M.;Liu, X.L.;Qin, G.Q.;Shen, W.
    • Asian-Australasian Journal of Animal Sciences
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    • 제14권5호
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    • pp.603-607
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    • 2001
  • This paper is based on the 36 wild Japanese quails which migrated to and settled in the Weishan Lake area. The gene frequency of 10 loci encoding the enzymes in viscera and muscle was detected. After collecting the same data about 20 quail colonies in China and other countries, it clusters the 21 quail populations by fuzzy cluster analysis. The study indicates that the wild Japanese quail in the Weishan Lake area is closer to domestic quail for phylogenetic system than wild Japanese quails in Japanese Islands. The paper supports the thesis that the quail domestication area should be further studied.

한국산 살치속(Hemiculter) 어류의 분자계통분류학적 위치 (Molecular Phylogenetic Status of Korean Hemiculter Species)

  • 김맹진;이재성;송춘복
    • 한국수산과학회지
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    • 제47권1호
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    • pp.72-78
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    • 2014
  • We investigated the molecular phylogeny and genetic differences among local populations of Korean Hemiculter fishes based on their mitochondrial cytochrome b gene sequences. Our results indicated that Hemiculter leucisculus populations in China were clearly divided into two groups. The first group (Group 1) included the populations of the Yangtze River and its surrounding areas (including the Qiantangjiang, Lingjiang, Jiulongjiang, and Minjiang rivers); the second group (Group 2) contained local populations from southern China (including the Nanliujiang, Zhujiang, Wanquanhe, Qianjiang, and Nandujiang rivers). The Korean Hemiculter eigenmanni differed in its cytochrome b gene sequence by 0.6-1.0% from the Chinese H. leucisculus (Group 1), which inhabited the Yangtze River and its surrounding areas, suggesting they were phylogenetically close and likely to be the same species. The Korean H. leucisculus differed from the Chinese H. leucisculus (both Groups 1 and 2) by 8.1-9.5%, indicating a very distant phylogenetic relationship; however, the Korean H. leucisculus differed from Hemiculter bleekeri by only 0.5-0.7%, showing intraspecific nucleotide differences. We conclude that the taxonomic relationship between the Korean H. leucisculus and H. bleekeri requires further investigation using type specimens.

핵(18S rDNA)과 엽록체 유전자(atpB) 분석을 통한 Staurastrum속과 Staurodesmus속 (Zygnematophyceae, Streptophyta)의 분자 계통학적 연구 (Molecular Phylogeny of the Genera Staurastrum and Staurodesmus (Zygnematophyceae, Streptophyta) Based on Nuclear (18S rDNA) and Chloroplast Gene (atpB) Sequences)

  • 문병렬;이옥민
    • ALGAE
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    • 제22권1호
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    • pp.1-10
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    • 2007
  • To gain insights into the phylogenetic relationships of genus Staurastrum and Staurodesmus, we analyzed nuclearencoded small subunit rDNA of 82 strains, and chloroplast atpB gene sequences of 44 strains belonging to three genera (Staurastrum, Staurodesmus, Cosmarium). Excluding the Staurastrum muticum and S. orbiculare, forty five strains of genus Staurastrum formed a well supported clade. It was shown that with no cell wall sculpture and processes, these two species have a strong phylogenetic relationship with genus Staurodesmus. Therefore, it is strongly recommended to transfer Staurastrum without processes and cell wall sculpture into Staurodesmus. S. obsoletus is a taxa that is transferred from Cosmarium. But, from this study, it has shown a phylogenetic relationship with Cosmarium. Therefore, this species is strongly recommended to transfer back to Cosmarium instead of Staurodesmus. As it was studied before, genus Staurastrum has shown monophyletic. Since the genus taurodesmus groups with Cosmarium, they were shown to be polyphyletic.

Evolutionary history of the monospecific Compsopogon genus (Compsopogonales, Rhodophyta)

  • Nan, Fangru;Feng, Jia;Lv, Junping;Liu, Qi;Xie, Shulian
    • ALGAE
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    • 제31권4호
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    • pp.303-315
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    • 2016
  • Compsopogon specimens collected in China were examined based on morphology and DNA sequences. Five molecular markers from different genome compartments including rbcL, COI, 18S rDNA, psbA, and UPA were identified and used to construct a phylogenetic relationship. Phylogenetic analyses indicated that two different morphological types from China clustered into an independent clade with Compsopogon specimens when compared to other global samples. The Compsopogon clade exhibited robust support values, revealing the affiliation of the samples to Compsopogon caeruleus. Although the samples were distributed in a close geographical area, unexpected sequence divergences between the Chinese samples implied that they were introduced by different dispersal events and from varied origins. It was speculated that Compsopogon originated in North America, a portion of the Laurentia landmass situated in the Rodinia supercontinent at approximately 573.89-1,701.50 million years ago during the Proterozoic era.Although Compsopogonhad evolved for a rather long time, genetic conservation had limited its variability and rate of evolution, resulting in the current monospecific global distribution. Additional global specimens and sequence information were required to increase our understanding of the evolutionary history of this ancient red algal lineage.