Browse > Article
http://dx.doi.org/10.7732/kjpr.2014.27.6.671

Phylogenetic Relationships in Korean Elaeagnus L. Based on nrDNA ITS Sequences  

Son, OGyeong (Department of Life Science, Yeungnam University)
Yoon, Chang Young (Department of Biotechnology, Shingyeong University)
Park, SeonJoo (Department of Life Science, Yeungnam University)
Publication Information
Korean Journal of Plant Resources / v.27, no.6, 2014 , pp. 671-679 More about this Journal
Abstract
Molecular phylogenetic analyses of Korean Elaeagnus L. were conducted using seven species, one variety, one forma and four outgroups to evaluate their relationships and phylogeny. The sequences of internal transcribed spacer regions in nuclear ribosomal DNA were employed to construct phylogenetic relationships using maximum parsimony (MP) and Bayesian analysis. Molecular phylogenetic analysis revealed that Korean Elaeagnus was a polyphyly. E. umbellata var. coreana formed a subclade with E. umbellata. Additionally, the genetic difference between E. submacrophylla and E. macrophylla was very low. Moreover, E. submacrophylla formed a branch from E. macrophylla, indicating that E. submacrophylla can be regarded as a variety. However, several populations of this species were not clustered as a single clade; therefore, further study should be conducted using other molecular markers. Although E. glabra f. oxyphylla was distinct in morphological characters of leaf shape with E. glabra. But E. glabra f. oxyphylla was formed one clade by molecular phylogenetic with E. glabra. Additionally, this study clearly demonstrated that E. pungens occurs in Korea, although it was previously reported near South Korea in Japan and China. According to the results of ITS regions analyses, it showed a resolution and to verify the relationship between interspecies of Korean Elaeagnus.
Keywords
Elaeagnus; Elaeagnaceae; ITS; Molecular phylogeny;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Chang, C.Y. 1983. Elaeagnus L.: Flora Reipublicae Popularis Sinicae, Science Press, Beijing, China. 52:1-60.
2 Ainouche, M.L. and R.J. Bayer. 1997. On the origins of the tetraploid Bromus species (section Bromus, Poaceae): insights from internal transcribed spacer sequences of nrDNA. Genome 40:730-743.   DOI
3 Baldwin, B.G. 1992. Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositae. Mol. Phylogent Evol. 1:3-16.   DOI   ScienceOn
4 Britton, N.L. and A. Brown. 1913. An Illustrated Flora of The Northern United States. Charles Scribner's Sons, New York, USA. 2:575.
5 Cronquist, A. 1981. An Intergrated System of Classification of Flowering Plants. Colurmbia Univ. Press. New York, USA. pp. 606-607.
6 Drummond, A.J., B. Ashton, S. Buston, M. Cheung, A. Cooper, C. Duran, M. Field, J. Heled, M. Kearse, R. Moir, S. Stones-Havas, S. Sturrock, T. Thierer and A. Wilson. 2011. Geneious v5.5, Available from http://www.geneious.com.
7 Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791.   DOI   ScienceOn
8 Fu, Y.C and X.J. Wang. 2007. Advances on chemical constituents and pharmacological activities from plants of Elaeagnus L. Qilu Pharmaceutical Affairs 26:232-233.
9 Hemsley, W.B. and F.B. Forbes. 1894. An enumeration of all the plants known form China (Index Florae Sinensis). Jour. Linn. Sco. Bot. 26:1-592.
10 Heywood, V.H., R.K. Brummitt, A. Culham and O. Seberg. 2007. Flowering plant families of the world. Kew Publishing. London, UK. pp. 135-136.
11 Kaplan, Z and J. Fehrer. 2007. Molecular evidence for a natural primary triple hybrid on plants revealed from direct sequencing. Ann. Bot. 99:1213-1222.   DOI   ScienceOn
12 Kim S.A., S.I. Oh and M.S. Lee. 2007. Antioxidative and cytotoxic effects of solvents fractions from Elaeagnus multiflora. Korean J. Food Nutr. 20:134-142 (in Korean).
13 Lee, D.B. 2006. Taxonomic Study on the Genus Elaeagnus in Korea. Department of Life Science, M.S Thesis, Hannam Univ., Korea (in Korean).
14 Kim, K.J. and R.K. Jansen. 1994. Comparisons of phylogenetic hypotheses among the different data sets in dwarf dadelion (krigia, Asteraceae): Additional information from internal transcribed spacer sequences of nuclear ribosomal DNA. Plant Syst. Evol. 190:157-185.   DOI   ScienceOn
15 Koh, J.E. 2005. A taxonomic study of the Korean Elaeagnus L.(Elaeagnaceae). Department of Life Science, M.S. Thesis, Sungkyunkwan Univ., Korea (in Korean).
16 Lee, C.H., J. Bae and J. Park. 2011. Pharmacognostical studies on the Korean folk medicien 'BoRiSuNaMu'. Kor. J. Pharmacogn. 42:1-5 (in Korean).
17 Lee, J.H. and W. Seo. 2011. Determination of phytochemical contents and biological activities from the fruits of Elaeagnus multiflora. J. Food Sci Nutr. 16:29-36   과학기술학회마을   DOI   ScienceOn
18 Lee, S.T. 2007. Elaeagnus: In Park, C.W. (ed.), The Genera of Vascular Plants of Korea, Flora of Korea Editorial Committee, Seoul, Korea (in Korean).
19 Lee, T.B. 1980. Illustrated Flora of Korea. Hyangmunsa, Publishing Co., Seoul, Korea. pp. 561-562 (in Korean).
20 Lee, W.T. 1996. Lineamenta Florae Koreae. Academy Publishing Co., Seoul, Korea. pp. 713-716 (in Korean).
21 Linnaeus, C. 1753. Species Plantarum. p. 121.
22 Loockerman, D.J. and R.K. Jansen. 1996. The use of herbarium material for DNA studies: In Stussey, T.J. and S. Sohmer (eds.), Sampling the Green World, Columbia Univ. Press, New York, USA. pp. 205-220.
23 Nakai, T. 1952. Elaeagnus. In A Synoptical Sketch of Korean Flora. Bull. Natl. Sci. Mus. Tokyo, Japan. pp. 31-75.
24 Mcneill, J., F.R. Rarrie, W.R. Buck, V. Demoulin, W. Greuter, D.L. Hawksworth, P.S. Herendeen, S. Knapp, K. Marhold, J. Prado, W.F. Prudhomme Van Reine, G.F. Smith and J.H. Wiersema. 2012. International Code of Botanical Nemenclature for Algae, Fungi and Plant (Melbourne Code). A. R. G. Gantner Verlag, Rugell, Liechtenstein. pp. 57-58.
25 Mori, T. 1921. An Enumertion of Plants Hitherto Known from Corea. Gov. Chos. pp. 259-260.
26 Nakai, T. 1911. Elaeagnus. Flora Koreana. Vol. II. J. Coll. Sci. Imp. Univ. Tokyo, Japan. 31:178-179.
27 Ohba, H. 2003. Flora of Japan. Vol. IIc. Kodansha. Tokyo, Japan. pp. 152-158.
28 Ohwi, J. 1984. Elaeagnus. In Flora off Japan. Smithsonian Institution. Washington, USA. pp. 646-648.
29 Palibin, J. 1900. Conspectus florae. Pars. secunda. trudy Imo. S. -Peterburgsk. Bot. Sada. 18:186-187.
30 Servettaz, C. 1908. Note preliminaire sur la Systematique des Elaeagnacees, Bull. de l'Herb. Boiss. 2. serie Tome VIII. pp. 381-394.
31 Ronquist, F., Huelsenbeck. 2003. MrBayes3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572-1574   DOI   ScienceOn
32 Schlechtendal DFL. 1857. Elaeagnaceae. Prodromus Systematis Naturalis Regni Vegetabilis 14:606-616.
33 Schlechtendal DFL. 1860. Elaeagnacearum in Candollei prodromo (Vol. XIV) expositarum adumbrates. Linnaea 30:304-386.
34 Servettaz, C. 1909. Monographie des Elaeagnacees in Beihefte Botanischen Centralbatt 25:1-128.
35 Wang, Y., P. Zhao, Y. Wang and Y. Zhang. 2006. Nutritional composition of wild Elaeagnus angustifolia fruits. Journal of Gansu Agricultural Univ. China. 41:6-28.
36 Sun, M. and Q. Lin. 2010. A revision of Elaeagnus L. (Elaeagnaceae) in mainland China. J. Syst. Evol. 48:356-390.   DOI
37 Sun, X. Chen, R. Ma, C. Li, Q. Wang and S. Ge. 2002. Molecular phylogenetics of Hippophae L.(Elaeagnaceae) based on the internal transcribed spacer (ITS) sequences of nrDNA. Plant Syst. Evol. 235:121-134.   DOI   ScienceOn
38 Swofford, D.L. 2003. $PAUP^*$: Phylogenetic Analysis Using Parsimony (*andothermethods). Version.4. Sinauer Associates, Sunderland, Massachusetts, USA.
39 White, T.J., T. Birns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics: In Gelfand, D., J. Sninsky and T. White (eds.), PCR Protocols: A guide to methods and applications. Academic Press, San Diego, USA. pp. 315-322.
40 Yoo K.P. and S.J. Park. 2012. A phlogenetic study of Korean Carpesium L. based on nrDNA ITS sequences. J. Korean Plant Res. 25:96-104 (in Korean).   DOI
41 Ki, K.R. 2004. Contribution of trichome morphology to the taxonomy of Korean Elaeagnus species. Department of Life Science, M.S. Thesis, Sungkyunkwan Univ., Korea (in Korean).
42 Nakai, T. 1916. Elaeagnus Japoniae, Coreae et Formosae. Bot. Mag. Tokyo, Japan. 30:72-76.   DOI